Nucleic acids and proteins from cenarchaeum symbiosum

ABSTRACT

The present application relates to nucleic acids and polypeptides from  Cenarchaeum symbiosum.  Methods of making the polypeptides and antibodies against the polypeptides are also described.

RELATED APPLICATIONS

[0001] The present application is a divisional of co-pending U.S. patent application Ser. No. 09/408,020, filed Sep. 29, 1999, which claims priority from U.S. Provisional Patent Application Serial No. 60/102,294, filed Sep. 29, 1998, the disclosure of which is incorporated herein by reference in its entirety.

BACKGROUND OF THE INVENTION

[0002] The identification and characterization of organisms which inhabit a diverse range of ecosystems leads to a greater understanding of the operation of such ecosystems. In addition, because the physiology of such organisms is adapted to function in the particular habitat which the organism inhabits, the enzymes which carry out the organism's physiological processes may possess characteristics which provide advantages when they are utilized in therapeutic procedures, industrial applications, or research applications. Furthermore, by determining the sequences of these organisms' genes, insight into their biochemical pathways and processes may be gained without the necessity of culturing the organisms in the laboratory, thereby enabling the physiological characterization of organisms which are recalcitrant to growth in the laboratory. Molecular phylogenetic surveys have recently revealed an ecologically widespread Crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, so their phenotypic and genotypic characteristics remain largely unknown. In order to characterize the physiology of these archaea, to develop methodological approaches for characterizing uncultivated microorganisms and identifying their presence in a sample, and to identify enzymes produced by these archae which may be useful in therapeutic, industrial, or laboratory applications, genomic analyses of the non-thermophilic crenarchaeote Cenarchaeum symbiosum was undertaken.

[0003] Non-thermophilic Crenarchaeota are one of the more abundant, widespread and frequently recovered prokaryotic groups revealed by molecular phylogenetic approaches. These microorganisms were originally detected in high abundance in temperate ocean waters and polar seas. (DeLong, E. F. 1992. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89, 5685-5689; DeLong, E. F et al. 1994. High abundance of Archaea in Antarctic marine picoplankton. Nature 371, 695-697; Fuhrman, J. A., et al. Davis. 1992. Novel major archaebacterial group from marine plankton. Nature 356, 148-149; Massana, R., et al. 1997. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Appl. Env. Microb. 63, 50-56; McInerney, J. O. et al. 1995. Recovery and phylogenetic analysis of novel archaeal rRNA sequences from a deep-sea deposit feeder. Appl. Env. Microb. 61, 1646-1648; Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246) Representatives have now been reported in terrestrial environments and freshwater lake sediments, indicating a widespread distribution. (Bintrim, S. B. et al. 1997. Molecular phylogeny of Archaea from soil. Proc. Natl. Acad. Sci. USA 94, 277-282; Jurgens, G. et al. 1997. Novel group within the kingdom Crenarchaeota from boreal forest soil. Appl. Env. Mircob. 63, 803-80515, Kudo, Y. et al 1997. Peculiar archaea found in Japanese paddy soils. Biosc. Biotech. Biochem. 61, 917-920; Ueda, et al. 1995. Molecular phylogenetic analysis of a soil microbial community. Eur. J. Soil Sci. 46, 415-421; Hershberger, K. L. et al. 1996. Wide diversity of Crenarchaeota. Nature 384, 420; MacGregor, B. J. 1997. Crenarchaeota in Lake Michigan sediment. Appl. Env. Microb. 63, 1178-1181 et al; Schleper, C. et al. 1997. Recovery of crenarchaeotal ribosomal DNA sequences from freshwater-lake sediments. Appl. Env. Microb. 63, 321-323) The ecological distribution of these organisms was initially surprising, since their closest cultivated relatives are all thermophilic or hyperthermophilic. No representative of this new archaeal group has yet been obtained in pure culture, so the phenotypic and metabolic properties of these organisms, as well as their impact on the environment and global nutrient cycling, remain unknown. Since growth temperature and habitat characteristics vary so widely between non-thermophilic and the hyperthermophilic Creanarchaeota, these groups are likely to differ greatly with respect to their specific physiology and metabolism.

[0004] To gain a better perspective on the genetic and physiological characteristics of non-thermophilic crenarchaeotes, a genomic study of Cenarchaeum symbiosum was begun. This archaeon lives in specific association with the marine sponge Axinella mexicana off the coast of California, allowing access to relatively large amounts of biomass from this species. (Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246) The approach taken herein differs in several respects from now standard genomic characterization of cultivated organisms, and also from comparable studies of uncultivated obligate parasites or symbionts. C. symbiosum has not been completely physically separated from the tissues of its metazoan host. Therefore, its genetic material needs to be identified within the context of complex genomic libraries that contain significant amounts of eucaryotic DNA, as well as DNA derived from members of Bacteria.

[0005] Molecular phylogenetic surveys of mixed microbial populations have revealed the existence of many new lineages undetected by classical microbiological approaches. (DeLong, E. F. 1997. Marine microbial diversity: the tip of the iceberg. Tibtech 15, 2-9.; Pace, N. R. 1997. A molecular view of microbial diversity and the biosphere. Science 276, 734-740) Furthermore, quantitative rRNA hybridization experiments demonstrate that some of these novel prokaryotic groups represent major components of natural microbial communities. These molecular phylogenetic approaches have altered current views of microbial diversity and ecology, and have demonstrated that traditional cultivation techniques may recover only a small, skewed fraction of naturally occurring microbes. However, phylogenetic identification using single gene sequences provides a limited perspective on other biological properties, particularly for novel lineages only distantly related to cultivated and characterized organisms. Consequently, additional approaches are necessary to better characterize ecologically abundant and potentially biotechnologically useful microorganisms, many of which resist cultivation attempts.

SUMMARY OF THE INVENTION

[0006] One embodiment of the present invention is an isolated, purified, or enriched nucleic acid comprising a sequence selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2, the sequences complementary to SEQ ID NO: 1 and SEQ ID NO: 2, fragments comprising at least 10 consecutive nucleotides of SEQ ID NO: 1 and SEQ ID NO: 2, and fragments comprising at least 10 consecutive nucleotides of the sequences complementary to SEQ ID NO: 1 and SEQ ID NO: 2. One aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of high stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of moderate stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of low stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 70% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 99% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters.

[0007] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOs: 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79 and the sequences complementary thereto. One aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of high stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of moderate stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of low stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 70% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 99% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters.

[0008] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid comprising at least 10 consecutive bases of a sequence selected from the group consisting of SEQ ID NOs: 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79 and the sequences complementary thereto. One aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 70% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters.

[0009] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOs: 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73, 77 and the sequences complementary thereto. One aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of high stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of moderate stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid capable of hybridizing to the nucleic acid of this embodiment under conditions of low stringency. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 70% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 99% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters.

[0010] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid comprising at least 10 consecutive bases of a sequence selected from the group consisting of SEQ ID NOs: 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73, 77 and the sequences complementary thereto. One aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 70% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters. Another aspect of the present invention is an isolated, purified, or enriched nucleic acid having at least 99% homology to the nucleic acid of this embodiment as determined by analysis with BLASTN version 2.0 with the default parameters.

[0011] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid encoding a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80.

[0012] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid encoding a polypeptide comprising at least 10 consecutive amino acids of a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80.

[0013] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid encoding a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0014] Another embodiment of the present invention is an isolated, purified, or enriched nucleic acid encoding a polypeptide comprising at least 10 consecutive amino acids of a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0015] Another embodiment of the present invention is an isolated or purified polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80. Another aspect of the present invention is an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment. Another aspect of the present invention is an isolated or purified polypeptide having at least 70% homology to the polypeptide of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is an isolated or purified polypeptide having at least 99% homology to the polypeptide of this emobdiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is an isolated or purified polypeptide having at least 70% homology to an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is an isolated or purified polypeptide having at least 99% homology to the polypeptide of to an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters.

[0016] Another aspect of the present invention is an isolated or purified polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. One aspect of the present invention is an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment. Another aspect of the present invention is an isolated or purified polypeptide having at least 70% homology to the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is an isolated or purified polypeptide having at least 99% homology to the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is An isolated or purified polypeptide having at least 70% homology to an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters. Another aspect of the present invention is an isolated or purified polypeptide having at least 99% homology to an isolated or purified polypeptide comprising at least 10 consecutive amino acids of the polypeptides of this embodiment as determined by analysis with FASTA version 3.0t78 with the default parameters.

[0017] Another embodiment of the present invention is an isolated or purified antibody capable of specifically binding to a polypeptide comprising a sequence selected from the group consisting of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80.

[0018] Another embodiment of the present invention is an isolated or purified antibody capable of specifically binding to a polypeptide comprising at least 10 consecutive amino acids of one of the polypeptides of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80.

[0019] Another embodiment of the present invention is an isolated or purified antibody capable of specifically binding to a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0020] Another embodiment of the present invention is an isolated or purified antibody capable of specifically binding to a polypeptide comprising at least 10 consecutive amino acids of one of the polypeptides of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0021] Another embodiment of the present invention is a method of making a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.

[0022] Another embodiment of the present invention is a method of making a polypeptide comprising at least 10 amino acids of a sequence selected from the group consisting of the sequences of SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.

[0023] Another embodiment of the present invention is a method of making a polypeptide having a sequence selected from the group consisting of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.

[0024] Another embodiment of the present invention is a method of making a polypeptide comprising at least 10 amino acids of a sequence selected from the group consisting of the sequences of SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising introducing a nucleic acid encoding said polypeptide, said nucleic acid being operably linked to a promoter, into a host cell.

[0025] Another embodiment of the present i method of generating a variant comprising obtaining a nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, the sequences complementary to the sequences of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, fragments comprising at least 30 consecutive nucleotides of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, and fragments comprising at least 30 consecutive nucleotides of the sequences complementary to SEQ ID NOS. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and changing one or more nucleotides in said sequence to another nucleotide, deleting one or more nucleotides in said sequence, or adding one or more nucleotides to said sequence. In one aspect of the present invention, the method further comprises the step of testing the enzymatic properties of a translation product of said variant.

[0026] Another embodiment of the present invention is a computer readable medium having stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0027] Another embodiment of the present invention is a computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. In one aspect of the present invention, the computer system further comprises a sequence comparer and a data storage device having reference sequences stored thereon. For example, the sequence comparer may comprise a computer program which indicates polymorphisms. In another aspect of the present invention is the computer system of this embodiment further comprises an identifier which identifies features in said sequence.

[0028] Another embodiment of the present invention is a method for comparing a first sequence to a reference sequence wherein said first sequence is selected from the group consisting of a nucleic acid code of SEQID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising the steps of reading said first sequence and said reference sequence through use of a computer program which compares sequences; and determining differences between said first sequence and said reference sequence with said computer program. In one aspect of the present invention, the step of determining differences between the first sequence and the reference sequence comprises identifying polymorphisms.

[0029] Another embodiment of the present invention is a method for identifying a feature in a sequence selected from the group consisting of a nucleic acid code of SEQID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising the steps of reading said sequence through the use of a computer program which identifies features in sequences and identifying features in said sequence with said computer program.

BRIEF DESCRIPTION OF THE DRAWINGS

[0030]FIG. 1 shows the locations of coding regions, the %G-C. and the %DNA identity between the approximately 28 Kb of common sequence in fosmids 101G10 and 60A5.

[0031]FIG. 2 shows the sequences surrounding the TATA boxes of several promoters from Cenarchaeum symbiosum and the distances from the TATA boxes to the initiation codons in these sequences.

[0032]FIG. 3 is a block diagram of an exemplary computer system.

[0033]FIG. 4 is a flow diagram illustrating one embodiment of a process 200 for comparing a new nucleotide or protein sequence with a database of sequences in order to determine the homology levels between the new sequence and the sequences in the database.

[0034]FIG. 5 is a flow diagram illustrating one embodiment of a process 250 in a computer for determining whether two sequences are homologous.

[0035]FIG. 6 is a flow diagram illustrating one embodiment of an identifier process for detecting the presence of a feature in a sequence.

DEFINITIONS

[0036] The term “gene” means the segment of DNA involved in producing a polypeptide chain; it includes regions preceding and following the coding region (leader and trailer) as well as, where applicable, intervening sequences (introns) between individual coding segments (exons).

[0037] As used herein, the term “isolated” means that the material is removed from its original environment (e.g., the natural environment if it is naturally occurring). For example, a naturally-occurring polynucleotide or polypeptide present in a living animal is not isolated, but the same polynucleotide or polypeptide, separated from some or all of the coexisting materials in the natural system, is isolated. Such polynucleotides could be part of a vector and/or such polynucleotides or polypeptides could be part of a composition, and still be isolated in that such vector or composition is not part of its natural environment.

[0038] As used herein, the term “purified” does not require absolute purity; rather, it is intended as a relative definition. Individual nucleic acids obtained from a library have been conventionally purified to electrophoretic homogeneity. The sequences obtained from these clones could not be obtained directly either from the library or from total human DNA. The purified nucleic acids of the present invention have been purified from the remainder of the genomic DNA in the organism by at least 10⁴-10⁶ fold. However, the term “purified” also includes nucleic acids which have been purified from the remainder of the genomic DNA or from other sequences in a library or other environment by at least one order of magnitude, preferably two or three orders, and more preferably four or five orders of magnitude.

[0039] As used herein, the term “recombinant” means that the nucleic acid is adjacent to “backbone” nucleic acid to which it is not adjacent in its natural environment. Additionally, to be “enriched” the nucleic acids will represent 5% or more of the number of nucleic acid inserts in a population of nucleic acid backbone molecules. Backbone molecules according to the present invention include nucleic acids such as expression vectors, self-replicating nucleic acids, viruses, integrating nucleic acids, and other vectors or nucleic acids used to maintain or manipulate a nucleic acid insert of interest. Preferably, the enriched nucleic acids represent 15% or more of the number of nucleic acid inserts in the population of recombinant backbone molecules. More preferably, the enriched nucleic acids represent 50% or more of the number of nucleic acid inserts in the population of recombinant backbone molecules. In a highly preferred embodiment, the enriched nucleic acids represent 90% or more of the number of nucleic acid inserts in the population of recombinant backbone molecules.

[0040] A promoter sequence is “operably linked to” a coding sequence when RNA polymerase which initiates transcription at the promoter will transcribe the coding sequence into mRNA.

[0041] “Recombinant” polypeptides or proteins refer to polypeptides or proteins produced by recombinant DNA techniques; i.e., produced from cells transformed by an exogenous DNA construct encoding the desired polypeptide or protein. “Synthetic” polypeptides or protein are those prepared by chemical synthesis.

[0042] A DNA “coding sequence” or a “nucleotide sequence encoding” a particular polypeptide or protein, is a DNA sequence which is transcribed and translated into a polypeptide or protein when placed under the control of appropriate regulatory sequences.

[0043] “Plasmids” are designated by a lower case p preceded and/or followed by capital letters and/or numbers. The starting plasmids herein are either commercially available, publicly available on an unrestricted basis, or can be constructed from available plasmids in accord with published procedures. In addition, equivalent plasmids to those described herein are known in the art and will be apparent to the ordinarily skilled artisan.

[0044] “Digestion” of DNA refers to catalytic cleavage of the DNA with a restriction enzyme that acts only at certain sequences in the DNA. The various restriction enzymes used herein are commercially available and their reaction conditions, cofactors and other requirements were used as would be known to the ordinarily skilled artisan. For analytical purposes, typically 1 μg of plasmid or DNA fragment is used with about 2 units of enzyme in about 20 μl of buffer solution. For the purpose of isolating DNA fragments for plasmid construction, typically 5 to 50 μg of DNA are digested with 20 to 250 units of enzyme in a larger volume. Appropriate buffers and substrate amounts for particular restriction enzymes are specified by the manufacturer. Incubation times of about 1 hour at 37° C. are ordinarily used, but may vary in accordance with the supplier's instructions. After digestion the gel electrophoresis may be performed to isolate the desired fragment.

[0045] “Oligonucleotide” refers to either a single stranded polydeoxynucleotide or two complementary polydeoxynucleotide strands which may be chemically synthesized. Such synthetic oligonucleotides have no 5′ phosphate and thus will not ligate to another oligonucleotide without adding a phosphate with an ATP in the presence of a kinase. A synthetic oligonucleotide will ligate to a fragment that has not been dephosphorylated.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0046] In order to begin the characterization of Cenarchaeum symbiosum, a large region of the C. symbiosum genome was sequenced. In particular, two overlapping C. symbiosum-derived fosmid inserts of approximately 42 kb and 33 kb were sequenced. The sequences of the two fosmid inserts revealed that there are at least two major variants or strains of C. symbiosum that coexist inside the sponge tissues of a single sponge. This complexity of the C. symbiosum population was not detected in initial studies based solely on direct sequencing of PCR amplified SSU genes. (Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246) This natural variation would also have been lost upon isolation of a pure culture.

[0047] The Cenarchaeum symbiosum sequences obtained from the two fosmids containing overlapping genomic inserts are provided in the accompanying sequence listing and are identified as SEQ ID NO: 1 and SEQ ID NO: 2. The two fosmid sequences were not entirely identical in their overlapping portions but instead contained differences. Upon further investigation, it was discovered that the two fosmid sequences were derived from two different, but closely related, strains of Cenarchaeum symbiosum (called variant A and variant B) which may simultaneously inhabit a single sponge.

[0048] Within the sequences of the fosmid inserts, numerous open reading frames encoding polypeptides having homology to known proteins, as well as open reading frames encoding proteins which do not exhibit homology to known proteins, were identified. Homology was determined using the program FASTA with the default parameters. The polypeptides encoded by these sequences are identified in the accompanying sequence listing as SEQ ID NOs: 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76 and 80 (polypeptides with homology to known proteins) and SEQ ID NOs: 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74 and 78 (polypeptides without homology to known proteins). In addition, sequences encoding the 16S rRNA, the 23S rRNA and a tyrosine tRNAs were also identified.

[0049] One aspect of the present invention is an isolated, purified, or enriched nucleic acid comprising one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 the sequences complementary thereto, or a fragment comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases of one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto. The isolated, purified or enriched nucleic acids may comprise DNA, including cDNA, genomic DNA, and synthetic DNA. The DNA may be double-stranded or single-stranded, and if single stranded may be the coding strand or non-coding (anti-sense) strand. Alternatively, the isolated, purified or enriched nucleic acids may comprise RNA.

[0050] As discussed in more detail below, the isolated, purified, or enriched nucleic acids of one of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 may be used to prepare one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids of one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80.

[0051] Accordingly, another aspect of the present invention is an isolated, purified, or enriched nucleic acid which encodes one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids of one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80. The coding sequences of these nucleic acids may be identical to one of the coding sequences of one of the nucleic acids of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or a fragment thereof or may be different coding sequences which encode one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids of one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 as a result of the redundancy or degeneracy of the genetic code. The genetic code is well known to those of skill in the art and can be obtained, for example, on page 214 of B. Lewin, Genes VI, Oxford University Press, 1997, the disclosure of which is incorporated herein by reference.

[0052] The isolated, purified, or enriched nucleic acid which encodes one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, and 80 may include, but is not limited to: only the coding sequence of one of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79; the coding sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 and additional coding sequences, such as leader sequences or proprotein sequences; or the coding sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 and non-coding sequences, such as introns or non-coding sequences 5′ and/or 3′ of the coding sequence. Thus, as used herein, the term “polynucleotide encoding a polypeptide” encompasses a polynucleotide which includes only coding sequence for the polypeptide as well as a polynucleotide which includes additional coding and/or non-coding sequence.

[0053] Alternatively, the nucleic acid sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 may be mutagenized using conventional techniques, such as site directed mutagenesis, or other techniques familiar to those skilled in the art, to introduce silent changes into the polynucleotides of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79. As used herein, “silent changes” include, for example, changes which do not alter the amino acid sequence encoded by the polynucleotide. Such changes may be desirable in order to increase the level of the polypeptide produced by host cells containing a vector encoding the polypeptide by introducing codons or codon pairs which occur frequently in the host organism.

[0054] The present invention also relates to polynucleotides which have nucleotide changes which result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80. Such nucleotide changes may be introduced using techniques such as site directed mutagenesis, random chemical mutagenesis, exonuclease III deletion, and other recombinant DNA techniques. Alternatively, such nucleotide changes may be naturally occurring allelic variants which are isolated by identifying nucleic acids which specifically hybridize to probes comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases of one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto to nucleic acids from Cenarchaeum symbiosum or related organisms under conditions of high, moderate, or low strigency as provided herein.

[0055] The isolated, purified, or enriched nucleic acids of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79, the sequences complementary thereto, or a fragment comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases of one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto may also be used as probes to identify the presence of Cenarchaeum symbiosum in a biological sample. In such procedures, a biological sample potentially harboring Cenarchaeum symbiosum is obtained and nucleic acids are obtained from the sample. The nucleic acids are contacted with the probe under conditions which permit the probe to specifically hybridize to any complementary sequences from Cenarchaeum symbiosum which are present therein.

[0056] Where necessary, conditions which permit the probe to specifically hybridize to complementary sequences from Cenarchaeum symbiosum may be determined by placing the probe in contact with complementary sequences from Cenarchaeum symbiosum as well as control sequences which are not from Cenarchaeum symbiosis. In some analyses, the control sequences may be from organisms related to Cenarchaeum symbiosum. Alternatively, the control sequences may be from organisms which are not related to Cenarchaeum symbiosum. Hybridization conditions, such as the salt concentration of the hybridization buffer, the formamide concentration of the hybridization buffer, or the hybridization temperature, may be varied to identify conditions which allow the probe to hybridize specifically to nucleic acids from Cenarchaeum symbiosum.

[0057] If the sample contains nucleic acids from Cenarchaeum symbiosum, specific hybridization of the probe to the nucleic acids from Cenarchaeum symbiosum is then detected. Hybridization may be detected by labeling the probe with a detectable agent such as a radioactive isotope, a fluorescent dye or an enzyme capable of catalyzing the formation of a detectable product.

[0058] Many methods for using the labeled probes to detect the presence of nucleic acids from Cenarchaeum symbiosum in a sample are familiar to those skilled in the art. These include Southern Blots, Northern Blots, colony hybridization procedures, and dot blots. Protocols for each of these procedures are provided in Ausubel et al. Current Protocols in Molecular Biology, John Wiley 503 Sons, Inc. 1997 and Sambrook et al., Molecular Cloning: A Laboratory Manual 2d Ed., Cold Spring Harbor Laboratory Press, 1989, the entire disclosures of which are incorporated herein by reference.

[0059] Alternatively, more than one probe (at least one of which is capable of specifically hybridizing to any complementary sequences from Cenarchaeum symbiosum which are present in the nucleic acid sample), may be used in an amplification reaction to determine whether the nucleic acid sample contains nucleic acids from Cenarchaeum symbiosum. Preferably, the probes comprise oligonucleotides. In one embodiment, the amplification reaction may comprise a PCR reaction. PCR protocols are described in Ausubel and Sambrook, supra. Alternatively, the amplification may comprise a ligase chain reaction, 3SR, or strand displacement reaction. (See Barany, F., “The Ligase Chain Reaction in a PCR World”, PCR Methods and Applications 1:5-16 (1991); E. Fahy et al., “Self-sustained Sequence Replication (3SR): An Isothermal Transcription-based Amplification System Alternative to PCR”, PCR Methods and Applications 1:25-33 (1991); and Walker G. T. et al., “Strand Displacement Amplification-an Isothermal in vitro DNA Amplification Technique, Nucleic Acid Research 20:1691-1696 (1992) the disclosures of which are incorporated herein by reference in their entireties). In such procedures, the nucleic acids in the sample are contacted with the probes, the amplification reaction is performed, and any resulting amplification product is detected. The amplification product may be detected by performing gel electrophoresis on the reaction products and staining the gel with an interculator such as ethidium bromide. Alternatively, one or more of the probes may be labeled with a radioactive isotope and the presence of a radioactive amplification product may be detected by autoradiography after gel electrophoresis.

[0060] Probes derived from sequences near the ends of the sequences of SEQ ID Nos: 1 and 2 may also be used in chromosome walking procedures to identify clones containing genomic sequences located adjacent to the sequences of SEQ ID Nos: 1 and 2. Such methods allow the isolation of genes which encode additional proteins expressed in Cenarchaeum symbiosum and facilitate the further physiological characterization of the organism.

[0061] Another aspect of the present invention is a method for determining whether a sample contains variant A and/or variant B of Cenarchaeum symbiosum. In such procedures, a sample potentially harboring variant A and/or variant B Cenarchaeum symbiosum is obtained and nucleic acids are obtained from the sample. The nucleic acids are contacted with the probe under conditions which permit the probe to specifically hybridize to any complementary sequences from variant A or variant B of Cenarchaeum symbiosum which are present therein. Preferably, the probe comprises a sequence having one or more nucleotides which differ between variant A and variant B. Conditions in which the probe specifically hybridizes to nucleic acids from one of the variants but not to nucleic acids from the other variant may be determined by contacting the probe with its corresponding sequence from variant A and variant B and varying the hybridization conditions, such as the salt concentration of the hybridization buffer, the formamide concentration of the buffer, or the hybridization temperature, to identify conditions in which the probe hybridizes to the corresponding sequence from one variant but not to the corresponding sequence from the other variant. Hybridization of the probe to nucleic acids from the Cenarchaeum symbiosum variant is then detected using any of the procedures described above.

[0062] The isolated, purified or enriched nucleic acids of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79, the sequences complementary thereto, or a fragment comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases of one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto may be used as probes to identify and isolate cDNAs encoding the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80. In such procedures, a cDNA library is constructed from a sample containing Cenarchaeum symbiosum. The cDNA library is then contacted with a probe comprising a coding sequence, or a fragment of a coding sequence, encoding one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or a fragment thereof under conditions which permit the probe to specifically hybridize to sequences complementary thereto. cDNAs which hybridize to the probe are then detected and isolated. Procedures for preparing and identifying cDNAs are disclosed in Ausubel et al. Current Protocols in Molecular Biology, John Wiley 503 Sons, Inc. 1997 and Sambrook et al., Molecular Cloning: A Laboratory Manual 2d Ed., Cold Spring Harbor Laboratory Press, 1989, the disclosures of which are incorporated herein by reference.

[0063] The isolated, purified, or enriched nucleic acids of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79, the sequences complementary thereto, or a fragment comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases of one of the sequences of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto may be used as probes to identify and isolate related nucleic acids. In some embodiments, the related nucleic acids may be cDNAs or genomic DNAs from organisms other than Cenarchaeum symbiosum. For example, the other organisms may be organisms which are related to Cenarchaeum symbiosum. In such procedures, a nucleic acid sample containing nucleic acids from the related organism, such as a cDNA or genomic DNA library from the related organism, is contacted with the probe under conditions which permit the probe to specifically hybridize to related sequences. Hybridization of the probe to nucleic acids from the related organism is then detected using any of the methods described above.

[0064] Hybridization may be carried out under conditions of low stringency, moderate stringency or high stringency. As an example of nucleic acid hybridization, a polymer membrane containing immobilized denatured nucleic acids is first prehybridized for 30 minutes at 45° C. in a solution consisting of 0.9 M NaCl, 50 mM NaH₂PO₄, pH 7.0, 5.0 mM Na₂EDTA, 0.5% SDS, 10× Denhardt's, and 0.5 mg/ml polyriboadenylic acid. Approximately 2×10⁷ cpm (specific activity 4-9×10⁸ cpm/ug) of ³²P end-labeled oligonucleotide probe are then added to the solution. After 12-16 hours of incubation, the membrane is washed for 30 minutes at room temperature in 1× SET (150 mM NaCl, 20 mM Tris hydrochloride, pH 7.8, 1 mM Na₂EDTA) containing 0.5% SDS, followed by a 30 minute wash in fresh 1× SET at Tm-10° C. for the oligonucleotide probe. The membrane is then exposed to auto-radiographic film for detection of hybridization signals.

[0065] By varying the stringency of the hybridization conditions used to identify nucleic acids, such as cDNAs or genomic DNAs, which hybridize to the detectable probe, nucleic acids having different levels of homology to the probe can be identified and isolated. Stringency may be varied by conducting the hybridization at varying temperatures below the melting temperatures of the probes. The melting temperature of the probe may be calculated using the following formulas:

[0066] For probes between 14 and 70 nucleotides in length the melting temperature (Tm) is calculated using the formula: Tm=81.5+16.6(log [Na+])+0.41(fraction G+C)−(600/N) where N is the length of the probe.

[0067] If the hybridization is carried out in a solution containing formamide, the melting temperature may be calculated using the equation Tm=81.5+16.6(log [Na+])+0.41(fraction G+C)−(0.63% formamide)−(600/N) where N is the length of the probe.

[0068] Prehybridization may be carried out in 6×SSC, 5× Denhardt's reagent, 0.5% SDS, 100 μg denatured fragmented salmon sperm DNA or 6×SSC, 5× Denhardt's reagent, 0.5% SDS, 100 μg denatured fragmented salmon sperm DNA, 50% formamide. The formulas for SSC and Denhardt's solutions are listed in Sambrook et al., supra.

[0069] Hybridization is conducted by adding the detectable probe to the prehybridization solutions listed above. Where the probe comprises double stranded DNA, it is denatured before addition to the hybridization solution. The filter is contacted with the hybridization solution for a sufficient period of time to allow the probe to hybridize to cDNAs or genomic DNAs containing sequences complementary thereto or homologous thereto. For probes over 200 nucleotides in length, the hybridization may be carried out at 15-25° C. below the Tm. For shorter probes, such as oligonucleotide probes, the hybridization may be conducted at 5-10° C. below the Tm. Preferably, for hybridizations in 6×SSC, the hybridization is conducted at approximately 68° C. Preferably, for hybridizations in 50% formamide containing solutions, the hybridization is conducted at approximately 42° C.

[0070] All of the foregoing hybridizations would be considered to be under conditions of high stringency.

[0071] Following hybridization, the filter is washed in 2×SSC, 0.1% SDS at room temperature for 15 minutes. The filter is then washed with 0.1×SSC, 0.5% SDS at room temperature for 30 minutes to 1 hour. Thereafter, the solution is washed at the hybridization temperature in 0.1×SSC, 0.5% SDS. A final wash is conducted in 0.1×SSC at room temperature.

[0072] Nucleic acids which have hybridized to the probe are identified by autoradiography or other conventional techniques.

[0073] The above procedure may be modified to identify nucleic acids having decreasing levels of homology to the probe sequence. For example, to obtain nucleic acids of decreasing homology to the detectable probe, less stringent conditions may be used. For example, the hybridization temperature may be decreased in increments of 5° C. from 68° C. to 42° C. in a hybridization buffer having a Na+ concentration of approximately 1M. Following hybridization, the filter may be washed with 2×SSC, 0.5% SDS at the temperature of hybridization. These conditions are considered to be “moderate” conditions above 50° C. and “low” conditions below 50° C. A specific example of “moderate” hybridization conditions is when the above hybridization is conducted at 55° C. A specific example of “low stringency” hybridization conditions is when the above hybridization is conducted at 45° C.

[0074] Alternatively, the hybridization may be carried out in buffers, such as 6×SSC, containing formamide at a temperature of 42° C. In this case, the concentration of formamide in the hybridization buffer may be reduced in 5% increments from 50% to 0% to identify clones having decreasing levels of homology to the probe. Following hybridization, the filter may be washed with 6×SSC, 0.5% SDS at 50° C. These conditions are considered to be “moderate” conditions above 25% formamide and “low” conditions below 25% formamide. A specific example of “moderate” hybridization conditions is when the above hybridization is conducted at 30% formamide. A specific example of “low stringency” hybridization conditions is when the above hybridization is conducted at 10% formamide.

[0075] Nucleic acids which have hybridized to the probe are identified by autoradiography.

[0076] For example, the preceding methods may be used to isolate nucleic acids having a sequence with at least 97%, at least 95%, at least 90%, at least 85%, at least 80%, or at least 70% homology to a nucleic acid sequence selected from the group consisting of one of the sequences of SEQ ID NOS. 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79, fragments comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive bases thereof, and the sequences complementary thereto. Homology may be measured using BLASTN version 2.0 with the default parameters. For example, the homologous polynucleotides may have a coding sequence which is a naturally occurring allelic variant of one of the coding sequences described herein. Such allelic variants may have a substitution, deletion or addition of one or more nucleotides when compared to the nucleic acids of SEQ ID NOs: 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77 and 79 or the sequences complementary thereto.

[0077] Additionally, the above procedures may be used to isolate nucleic acids which encode polypeptides having at least 99%, 95%, at least 90%, at least 85%, at least 80%, or at least 70% homology to a polypeptide having the sequence of one of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof as determined using the FASTA version 3.0t78 algorithm with the default parameters.

[0078] Another aspect of the present invention is an isolated or purified polypeptide comprising the sequence of one of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof. As discussed above, such polypeptides may be obtained by inserting a nucleic acid encoding the polypeptide into a vector such that the coding sequence is operably linked to a sequence capable of driving the expression of the encoded polypeptide in a suitable host cell. For example, the expression vector may comprise a promoter, a ribosome binding site for translation initiation and a transcription terminator. The vector may also include appropriate sequences for amplifying expression.

[0079] Promoters suitable for expressing the polypeptide or fragment thereof in bacteria include the E. coli. lac or trp promoters, the lacI promoter, the lacZ promoter, the T3 promoter, the T7 promoter, the gpt promoter, the lambda P_(R) promoter, the lambda P_(L) promoter the trp promoter, promoters from operons encoding glycolytic enzymes such as 3-phosphoglycerate kinase (PGK), and the acid phosphatase promoter. Fungal promoters include the α factor promoter. Eukaryotic promoters include the CMV immediate early promoter, the HSV thymidine kinase promoter, heat shock promoters, the early and late SV40 promoter, LTRs from retroviruses, and the mouse metallothionein-I promoter. Other promoters known to control expression of genes in prokaryotic or eukaryotic cells or their viruses may also be used.

[0080] Mammalian expression vectors may also comprise an origin of replication, any necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5′ flanking nontranscribed sequences. In some embodiments, DNA sequences derived from the SV40 splice and polyadenylation sites may be used to provide the required nontranscribed genetic elements.

[0081] Vectors for expressing the polypeptide or fragment thereof in eukaryotic cells may also contain enhancers to increase expression levels. Enhancers are cis-acting elements of DNA, usually from about 10 to about 300 bp in length that act on a promoter to increase its transcription. Examples include the SV40 enhancer on the late side of the replication origin bp 100 to 270, the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and the adenovirus enhancers.

[0082] In addition, the expression vectors preferably contain one or more selectable marker genes to permit selection of host cells containing the vector. Such selectable markers include genes encoding dihydrofolate reductase or genes conferring neomycin resistance for eukaryotic cell culture, genes conferring tetracycline or ampicillin resistance in E. coli, and the S. cerevisiae TRP1 gene.

[0083] In some embodiments, the nucleic acid encoding one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof is assembled in appropriate phase with a leader sequence capable of directing secretion of the translated polypeptide or fragment thereof. Optionally, the nucleic acid can encode a fusion polypeptide in which one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof is fused to heterologous peptides or polypeptides, such as N-terminal identification peptides which impart desired characteristics, such as increased stability or simplified purification.

[0084] The appropriate DNA sequence may be inserted into the vector by a variety of procedures. In general, the DNA sequence is ligated to the desired position in the vector following digestion of the insert and the vector with appropriate restriction endonucleases. Alternatively, blunt ends in both the insert and the vector may be ligated. A variety of cloning techniques are disclosed in Ausubel et al. Current Protocols in Molecular Biology, John Wiley 503 Sons, Inc. 1997 and Sambrook et al., Molecular Cloning: A Laboratory Manual 2d Ed., Cold Spring Harbor Laboratory Press, 1989, the entire disclosures of which are incorporated herein by reference. Such procedures and others are deemed to be within the scope of those skilled in the art.

[0085] The vector may be, for example, in the form of a plasmid, a viral particle, or a phage. Other vectors include chromosomal, nonchromosomal and synthetic DNA sequences, derivatives of SV40; bacterial plasmids, phage DNA, baculovirus, yeast plasmids, vectors derived from combinations of plasmids and phage DNA, viral DNA such as vaccinia, adenovirus, fowl pox virus, and pseudorabies. A variety of cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described by Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., (1989), the disclosure of which is hereby incorporated by reference.

[0086] Particular bacterial vectors which may be used include the commercially available plasmids comprising genetic elements of the well known cloning vector pBR322 (ATCC 37017), pKK223-3 (Pharmacia Fine Chemicals, Uppsala, Sweden), GEM1 (Promega Biotec, Madison, Wis., USA) pQE70, pQE60, pQE-9 (Qiagen), pD10, psiX174 pBluescript II KS, pNH8A, pNH16a, pNH18A, pNH46A (Stratagene), ptrc99a, pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia), pKK232-8 and pCM7. Particular eukaryotic vectors include pSV2CAT, pOG44, pXT1, pSG (Stratagene) pSVK3, pBPV, pMSG, and pSVL (Pharmacia). However, any other vector may be used as long as it is replicable and viable in the host cell.

[0087] The host cell may be any of the host cells familiar to those skilled in the art, including prokaryotic cells, eukaryotic cells, mammalian cells, insect cells, or plant cells. As representative examples of appropriate hosts, there may be mentioned: bacterial cells, such as E. coli, Streptomyces, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus, fungal cells, such as yeast, insect cells such as Drosophila S2 and Spodoptera Sf9 animal cells such as CHO, COS or Bowes melanoma, and adenoviruses. The selection of an appropriate host is within the abilities of those skilled in the art.

[0088] The vector may be introduced into the host cells using any of a variety of techniques, including transformation, transfection, transduction, viral infection, gene guns, or Ti-mediated gene transfer. Particular methods include calcium phosphate transfection, DEAE-Dextran mediated transfection, lipofection, or electroporation (Davis, L., Dibner, M., Battey, I., Basic Methods in Molecular Biology, (1986)).

[0089] Where appropriate, the engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants or amplifying the genes of the present invention. Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter may be induced by appropriate means (e.g., temperature shift or chemical induction) and the cells may be cultured for an additional period to allow them to produce the desired polypeptide or fragment thereof.

[0090] Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract is retained for further purification. Microbial cells employed for expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents. Such methods are well known to those skilled in the art. The expressed polypeptide or fragment thereof can be recovered and purified from recombinant cell cultures by methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography and lectin chromatography. Protein refolding steps can be used, as necessary, in completing configuration of the polypeptide. If desired, high performance liquid chromatography (HPLC) can be employed for final purification steps.

[0091] Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts (described by Gluzman, Cell, 23:175 (1981), and other cell lines capable of expressing proteins from a compatible vector, such as the C127, 3T3, CHO, HeLa and BHK cell lines.

[0092] The constructs in host cells can be used in a conventional manner to produce the gene product encoded by the recombinant sequence. Depending upon the host employed in a recombinant production procedure, the polypeptides produced by host cells containing the vector may be glycosylated or may be non-glycosylated. Polypeptides of the invention may or may not also include an initial methionine amino acid residue.

[0093] Alternatively, the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof can be synthetically produced by conventional peptide synthesizers. In other embodiments, fragments or portions of the polypeptides may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, the fragments may be employed as intermediates for producing the full-length polypeptides.

[0094] Cell-free translation systems can also be employed to produce one of the polypeptides of SEQ ID Nos: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof using mRNAs transcribed from a DNA construct comprising a promoter operably linked to a nucleic acid encoding the polypeptide or fragment thereof. In some embodiments, the DNA construct may be linearized prior to conducting an in vitro transcription reaction. The transcribed mRNA is then incubated with an appropriate cell-free translation extract, such as a rabbit reticulocyte extract, to produce the desired polypeptide or fragment thereof.

[0095] The present invention also relates to variants of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof. The term “variant” includes derivatives or analogs of these polypeptides. In particular, the variants may differ in amino acid sequence from the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 by one or more substitutions, additions, deletions, fusions and truncations, which may be present in any combination.

[0096] The variants may be naturally occurring or created in vitro. In particular, such variants may be created using genetic engineering techniques such as site directed mutagenesis, random chemical mutagenesis, Exonuclease III deletion procedures, and standard cloning techniques. Alternatively, such variants, fragments, analogs, or derivatives may be created using chemical synthesis or modification procedures.

[0097] Other methods of making variants are also familiar to those skilled in the art. These include procedures in which nucleic acid sequences obtained from natural isolates are modified to generate nucleic acids which encode polypeptides having characteristics which enhance their value in industrial or laboratory applications. In such procedures, a large number of variant sequences having one or more nucleotide differences with respect to the sequence obtained from the natural isolate are generated and characterized. Preferably, these nucleotide differences result in amino acid changes with respect to the polypeptides encoded by the nucleic acids from the natural isolates.

[0098] For example, variants may be created using error prone PCR. In error prone PCR, PCR is performed under conditions where the copying fidelity of the DNA polymerase is low, such that a high rate of point mutations is obtained along the entire length of the PCR product. Error prone PCR is described in Leung, D. W., et al., Technique, 1:11-15 (19 89) and Caldwell, R. C. & Joyce G. F., PCR Methods Applic., 2:28-33 (1992), the disclosure of which is incorporated herein by reference in its entirety. Briefly, in such procedures, nucleic acids to be mutagenized are mixed with PCR primers, reaction buffer, MgCl₂, MnCl₂, Taq polymerase and an appropriate concentration of dNTPs for achieving a high rate of point mutation along the entire length of the PCR product. For example, the reaction may be performed using 20 fmoles of nucleic acid to be mutagenized, 30 pmole of each PCR primer, a reaction buffer comprising 50 mM KCl, 10 mM Tris HCl (pH 8.3) and 0.01% gelatin, 7 mM MgCl₂, 0.5 mM MnCl₂, 5 units of Taq polymerase, 0.2 mM dGTP, 0.2 mM dATP, 1 mM dCTP, and 1 mM dTTP. PCR may be performed for 30 cycles of 94° C. for 1 min, 45° C. for 1 min, and 72° C. for 1 min. However, it will be appreciated that these parameters may be varied as appropriate. The mutagenized nucleic acids are cloned into an appropriate vector and the activities of the polypeptides encoded by the mutagenized nucleic acids is evaluated.

[0099] Variants may also be created using oligonucleotide directed mutagenesis to generate site-specific mutations in any cloned DNA segment of interest. Oligonucleotide mutagenesis is described in Reidhaar-Olson, J. F. & Sauer, R. T., et al., Science, 241:53-57 (1988), the disclosure of which is incorporated herein by reference in its entirety. Briefly, in such procedures a plurality of double stranded oligonucleotides bearing one or more mutations to be introduced into the cloned DNA are synthesized and inserted into the cloned DNA to be mutagenized. Clones containing the mutagenized DNA are recovered and the activities of the polypeptides they encode are assessed.

[0100] Another method for generating variants is assembly PCR. Assembly PCR involves the assembly of a PCR product from a mixture of small DNA fragments. A large number of different PCR reactions occur in parallel in the same vial, with the products of one reaction priming the products of another reaction. Assembly PCR is described in U.S. patent application Ser. No. 08/677,112, filed Jul. 9, 1997 and U.S. patent application Ser. No. 08/942,504, filed Oct. 31, 1997, the disclosures of which are incorporated herein by reference in their entireties.

[0101] Still another method of generating variants is sexual PCR mutagenesis. In sexual PCR mutagenesis, forced homologous recombination occurs between DNA molecules of different but highly related DNA sequence in vitro, as a result of random fragmentation of the DNA molecule based on sequence homology, followed by fixation of the crossover by primer extension in a PCR reaction. Sexual PCR mutagenesis is described in Stemmer, W. P., PNAS, USA, 91:10747-10751 (1994), the disclosure of which is incorporated herein by reference. Briefly, in such procedures a plurality of nucleic acids to be recombined are digested with DNAse to generate fragments having an average size of 50-200 nucleotides. Fragments of the desired average size are purified and resuspended in a PCR mixture. PCR is conducted under conditions which facilitate recombination between the nucleic acid fragments. For example, PCR may be performed by resuspending the purified fragments at a concentration of 10-30 ng/μl in a solution of 0.2 mM of each dNTP, 2.2 mM MgCl2, 50 mM KCL, 10 mM Tris HCl, pH 9.0, and 0.1% Triton X-100. 2.5 units of Taq polymerase per 100 μl of reaction mixture is added and PCR is performed using the following regime: 94° C. for 60 seconds, 94° C. for 30 seconds, 50-55° C. for 30 seconds, 72° C. for 30 seconds (30-45 times) and 72° C. for 5 minutes. However, it will be appreciated that these parameters may be varied as appropriate. In some embodiments, oligonucleotides may be included in the PCR reactions. In other embodiments, the Klenow fragment of DNA polymerase I may be used in a first set of PCR reactions and Taq polymerase may be used in a subsequent set of PCR reactions. Recombinant sequences are isolated and the activities of the polypeptides they encode are assessed.

[0102] Variants may also be created by in vivo mutagenesis. In some embodiments, random mutations in a sequence of interest are generated by propagating the sequence of interest in a bacterial strain, such as an E. coli strain, which carries mutations in one or more of the DNA repair pathways. Such “mutator” strains have a higher random mutation rate than that of a wild-type parent. Propagating the DNA in one of these strains will eventually generate random mutations within the DNA. Mutator strains suitable for use for in vivo mutagenesis are described in PCT Published Application WO 91/16427, the disclosure of which is incorporated herein by reference in its entirety.

[0103] Variants may also be generated using cassette mutagenesis. In cassette mutagenesis a small region of a double stranded DNA molecule is replaced with a synthetic oligonucleotide “cassette” that differs from the native sequence. The oligonucleotide often contains completely and/or partially randomized native sequence.

[0104] Recursive ensemble mutagenesis may also be used to generate variants. Recursive ensemble mutagenesis is an algorithm for protein engineering (protein mutagenesis) developed to produce diverse populations of phenotypically related mutants whose members differ in amino acid sequence. This method uses a feedback mechanism to control successive rounds of combinatorial cassette mutagenesis. Recursive ensemble mutagenesis is described in Arkin, A. P. and Youvan, D. C., PNAS, USA, 89:7811-7815 (1992), the disclosure of which is incorporated herein by reference in its entirety.

[0105] In some embodiments, variants are created using exponential ensemble mutagenesis. Exponential ensemble mutagenesis is a process for generating combinatorial libraries with a high percentage of unique and functional mutants, wherein small groups of residues are randomized in parallel to identify, at each altered position, amino acids which lead to functional proteins. Exponential ensemble mutagenesis is described in Delegrave, S. and Youvan, D. C., Biotechnology Research, 11:1548-1552 (1993), the disclosure of which incorporated herein by reference in its entirety. Random and site-directed mutagenesis are described in Arnold, F. H., Current Opinion in Biotechnology, 4:450-455 (1993), the disclosure of which is incorporated herein by reference in its entirety.

[0106] In some embodiments, the variants are created using shuffling procedures wherein portions of a plurality of nucleic acids which encode distinct polypeptides are fused together to create chimeric nucleic acid sequences which encode chimeric polypeptides. Shuffling procedures are described in U.S. patent application Ser. No. 08/677,112, filed Jul. 9, 1996, U.S. patent application Ser. No. 08/942,504, filed Oct. 31, 1997, U.S. Pat. No. 5,939,250, issued Aug. 17, 1999, and U.S. patent application Ser. No. 09/375,605, filed Aug. 17, 1999, the disclosures of which are incorporated herein by reference in their entireties.

[0107] The variants of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 may be (i) variants in which one or more of the amino acid residues of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 are substituted with a conserved or non-conserved amino acid residue (preferably a conserved amino acid residue) and such substituted amino acid residue may or may not be one encoded by the genetic code.

[0108] Conservative substitutions are those that substitute a given amino acid in a polypeptide by another amino acid of like characteristics. Typically seen as conservative substitutions are the following replacements: replacements of an aliphatic amino acid such as Ala, Val, Leu and Ile with another aliphatic amino acid; replacement of a Ser with a Thr or vice versa; replacement of an acidic residue such as Asp and Glu with another acidic residue; replacement of a residue bearing an amide group, such as Asn and Gln, with another residue bearing an amide group; exchange of a basic residue such as Lys and Arg with another basic residue; and replacement of an aromatic residue such as Phe, Tyr with another aromatic residue.

[0109] Other variants are those in which one or more of the amino acid residues of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 includes a substituent group.

[0110] Still other variants are those in which the polypeptide is associated with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol).

[0111] Additional variants are those in which additional amino acids are fused to the polypeptide, such as a leader sequence, a secretory sequence, a proprotein sequence or a sequence which facilitates purification, enrichment, or stabilization of the polypeptide.

[0112] In some embodiments, the fragments, derivatives and analogs retain the same biological function or activity as the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80. In other embodiments, the fragment, derivative, or analog includes a proprotein, such that the fragment, derivative, or analog can be activated by cleavage of the proprotein portion to produce an active polypeptide.

[0113] Another aspect of the present invention are polypeptides or fragments thereof which have at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, or more than 95% homology to one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or a fragment comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof. Homology may be determined using a program, such as FASTA version 3.0t78 with the default parameters, which aligns the polypeptides or fragments being compared and determines the extent of amino acid identity or similarity between them. It will be appreciated that amino acid “homology” includes conservative amino acid substitutions such as those described above.

[0114] The polypeptides or fragments having homology to one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or a fragment comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof may be obtained by isolating the nucleic acids encoding them using the techniques described above.

[0115] Alternatively, the homologous polypeptides or fragments may be obtained through biochemical enrichment or purification procedures. The sequence of potentially homologous polypeptides or fragments may be determined by proteolytic digestion, gel electrophoresis and/or microsequencing. The sequence of the prospective homologous polypeptide or fragment can be compared to one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or a fragment comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof using a program such as FASTA version 3.0t78 with the default parameters.

[0116] The polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof invention may be used in a variety of applications. For example, the polypeptides or fragments thereof may be used to catalyze biochemical reactions. In particular, the polypeptides of SEQ ID NOs: 14 and 46, which have homology to glutamate semialdehyde amino transferase, or fragments thereof, may be used to catalyze the synthesis of 5-aminolevulinate from S-4-amino-5-oxopentanoate. The polypeptides of SEQ ID NOs: 26 and 58, which have homology to triose phosphate isomerase, or fragments thereof, may be used to catalyze the synthesis of glycerone phosphate from D-glyceraldehyde 3-phosphate. The polypeptides of SEQ ID NOs: 32 and 64, which have homology to dCMP deaminase, or fragments thereof, may be used to catalyze the reaction of deoxyctidine and water to produce deoxyuridine and ammonia. The polypeptides of SEQ ID NOs: 38 and 72, which have homology to the MenA protein, or fragments thereof, may be used to catalyze the synthesis of menaquinone. The polypeptide of SEQ ID NO: 80, which has homology to glucose-1-dehydrogenase, may be used to catalyze the synthesis of D-glucono-1,5-lacctone from D-glucose.

[0117] The polypeptide of SEQ ID NO: 10, which has homology to lysyl tRNA synthetase, or fragments thereof, may be used to identify compounds capable of specifically inhibiting the growth of Cenarchaeum symbiosis, since tRNA synthetases are attractive targets for agents which inhibit growth.

[0118] Agents which specifically inhibit the activity of the lysyl tRNA synthetase from Cenarchaeum symbiosum may be identified using a variety of methods known to those skilled in the art. For example, a plurality of agents may be generated using combinatorial chemistry or recombinant DNA libraries encoding a large number of short peptides. The lysyl tRNA synthetases from Cenarchaeum symbiosum and control organisms are contacted with the agents and those agents which bind to the lysyl tRNA synthetase from Cenarchaeum symbiosum but not to the enzyme from the control organisms are identified. Cenarchaeum symbiosum is then contacted with the identified agents to determine which agents inhibit the organism's growth.

[0119] The polypeptides of SEQ ID NOs: 28 and 60, which have homology to the TATA box binding protein, or fragments thereof, may be used to identify promoters in nucleic acids from Cenarchaeum symbiosis. In such procedures, the polypeptide or fragment thereof is allowed to contact the nucleic acid and binding of the polypeptide or fragment thereof to the nucleic acid is detected. Binding may be detected by performing a gel shift analysis, a nuclease protection analysis, or by detecting the retention of the nucleic acid on a column matrix having the TATA box binding protein, or a fragment thereof, affixed thereto.

[0120] Compounds which specifically inhibit the binding of the TATA box binding protein of Cenarchaeutm symbiosis to promoters may also be used to inhibit growth of the organism. Such compounds may be identified as described above.

[0121] Similarly, agents which specifically inhibit the activity of the polypeptides of SEQ ID NOs: 34 and 66, which have homology to RNA helicase, may be used to inhibit the growth of Cenarchaeum symbiosis. Such agents may be identified as described above.

[0122] The polypeptides of SEQ ID NOs: 30 and 62, which have homology to DNA polymerase I, or fragments thereof, may be used to insert a detectable label into a nucleic acid or to generate blunt ends on nucleic acids which have been digested with a restriction endonuclease.

[0123] The polypeptides of SEQ ID NOs: 42 and 76, which have homology to site specific DNA methyltranseferases, or fragments thereof, may be used in procedures in which it is desirable to protect nucleic acid sequences from digestion with restriction endonucleases. For example, a nucleic acid sequence having one or more restriction sites therein may be treated with the polypeptides of SEQ ID NOs: 42 or 76 prior to the addition of linkers to the nucleic acid. Thereafter, the linkers may be digested with the restriction enzyme, while the sites in the remainder of the nucleic acid are protected from digestion.

[0124] The polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80, or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof, may also be used to generate antibodies which bind specifically to the polypeptides or fragments. The resulting antibodies may be used to determine whether a biological sample contains Cenarchaeum symbiosum. In such procedures, a biological sample is contacted with an antibody capable of specifically binding to one of the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof. The ability of the biological sample to bind to the antibody is then determined. For example, binding may be determined by labeling the antibody with a detectable label such as a fluorescent agent, an enzymatic label, or a radioisotope. Alternatively, binding of the antibody to the sample may be detected using a secondary antibody having such a detectable label thereon. A variety of assay protocols which may be used to detect the presence of Cenarchaeum symbiosum in a sample are familiar to those skilled in the art. Particular assays include ELISA assays, sandwich assays, radioimmunoassays, and Western Blots.

[0125] Polyclonal antibodies generated against the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof can be obtained by direct injection of the polypeptides into an animal or by administering the polypeptides to an animal, preferably a nonhuman. The antibody so obtained will then bind the polypeptide itself. In this manner, even a sequence encoding only a fragment of the polypeptide can be used to generate antibodies which may bind to the whole native polypeptide. Such antibodies can then be used to isolate the polypeptide from cells expressing that polypeptide.

[0126] For preparation of monoclonal antibodies, any technique which provides antibodies produced by continuous cell line cultures can be used. Examples include the hybridoma technique (Kohler and Milstein, 1975, Nature, 256:495-497, the disclosure of which is incorporated herein by reference), the trioma technique, the human B-cell hybridoma technique (Kozbor et al., 1983, Immunology Today 4:72, the disclosure of which is incorporated herein by reference), and the EBV-hybridoma technique (Cole, et al., 1985, in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., pp. 77-96, the disclosure of which is incorporated herein by reference).

[0127] Techniques described for the production of single chain antibodies (U.S. Pat. No. 4,946,778, the disclosure of which is incorporated herein by reference) can be adapted to produce single chain antibodies to the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof. Alternatively, transgenic mice may be used to express humanized antibodies to these polypeptides or fragments thereof.

[0128] Antibodies generated against the polypeptides of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74 76, 78, and 80 or fragments comprising at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids thereof may be used in screening for similar polypeptides from other organisms and samples. In such techniques, polypeptides from the organism are contacted with the antibody and those polypeptides which specifically bind the antibody are detected. Any of the procedures described above may be used to detect antibody binding. One such screening assay is described in “Methods for Measuring Cellulase Activities”, Methods in Enzymology, Vol 160, pp. 87-116, which is hereby incorporated by reference in its entirety.

[0129] As used herein the term “nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77” encompasses the nucleotide sequences of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, fragments of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, nucleotide sequences homologous to SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or homologous to fragments of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, and sequences complementary to all of the preceding sequences. The fragments include portions of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 comprising at least 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, 150, 200, 300, 400, or 500 consecutive nucleotides of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77. Preferably, the fragments are novel fragments. Homologous sequences and fragments of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 refer to a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80%, 75% or 70% homology to these sequences. Homology may be determined using any of the computer programs and parameters described herein, including BLASTN version 2.0 with the default parameters. Homologous sequences also include RNA sequences in which uridines replace the thymines in the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77. The homologous sequences may be obtained using any of the procedures described herein or may result from the correction of a sequencing error. It will be appreciated that the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 can be represented in the traditional single character format (See the inside back cover of Stryer, Lubert. Biochemistry, 3^(rd) edition. W. H Freeman & Co., New York.) or in any other format which records the identity of the nucleotides in a sequence.

[0130] As used herein the term “polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78” encompasses the polypeptide sequence of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 which are encoded by the extended cDNAs of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, polypeptide sequences homologous to the polypeptides of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78, or fragments of any of the preceding sequences. Homologous polypeptide sequences refer to a polypeptide sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80%, 75% or 70% homology to one of the polypeptide sequences of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. Homology may be determined using any of the computer programs and parameters described herein, including FASTA version 3.0t78 with the default parameters or with any modified parameters. The homologous sequences may be obtained using any of the procedures described herein or may result from the correction of a sequencing error. The polypeptide fragments comprise at least 5, 10, 15, 20, 25, 30, 35, 40, 50, 75, 100, or 150 consecutive amino acids of the polypeptides of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. Preferably, the fragments are novel fragments. It will be appreciated that the polypeptide codes of the SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 can be represented in the traditional single character format or three letter format (See the inside back cover of Starrier, Lubert. Biochemistry, 3^(rd) edition. W. H Freeman & Co., New York.) or in any other format which relates the identity of the polypeptides in a sequence.

[0131] It will be appreciated by those skilled in the art that the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 can be stored, recorded, and manipulated on any medium which can be read and accessed by a computer. As used herein, the words “recorded” and “stored” refer to a process for storing information on a computer medium. A skilled artisan can readily adopt any of the presently known methods for recording information on a computer readable medium to generate manufactures comprising one or more of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77, one or more of the polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, 15, or 20 nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77.

[0132] Another aspect of the invention is a computer readable medium having recorded thereon one or more of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, and 79. Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, or 15 of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, and 79.

[0133] Another aspect of the present invention is a computer readable medium having recorded thereon one or more of the nucleic acid codes of SEQ ID NOs. 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77. Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, or 15 of SEQ ID NOs. 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77.

[0134] Another aspect of the present invention is a computer readable medium having recorded thereon one or more of the polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. Another aspect of the present invention is a computer readable medium having recorded thereon one or more of the the polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80. Another aspect of the present invention is a computer readable medium having recorded thereon one or more of the the polypeptide codes of SEQ ID NOS. 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0135] Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, 15, or 20 polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, or 15 polypeptide codes of SEQ ID NOS. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, and 80. Another aspect of the present invention is a computer readable medium having recorded thereon at least 2, 5, 10, or 15 polypeptide codes of SEQ ID NOS. 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0136] Computer readable media include magnetically readable media, optically readable media, electronically readable media and magnetic/optical media. For example, the computer readable media may be a hard disk, a floppy disk, a magnetic tape, CD-ROM, Digital Versatile Disk (DVD), Random Access Memory (RAM), or Read Only Memory (ROM) as well as other types of other media known to those skilled in the art.

[0137] Embodiments of the present invention include systems, particularly computer systems which store and manipulate the sequence information described herein. One example of a computer system 100 is illustrated in block diagram form in FIG. 3. As used herein, “a computer system” refers to the hardware components, software components, and data storage components used to analyze the nucleotide sequences of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the sequences of the polypeptide codes of 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. The computer system 100 preferably includes a processor for processing, accessing and manipulating the sequence data. The processor 105 can be any well-known type of central processing unit, such as the Pentium III from Intel Corporation, or similar processor from Sun, Motorola, Compaq or International Business Machines.

[0138] Preferably, the computer system 100 is a general purpose system that comprises the processor 105 and one or more internal data storage components 110 for storing data, and one or more data retrieving devices for retrieving the data stored on the data storage components. A skilled artisan can readily appreciate that any one of the currently available computer systems are suitable.

[0139] In one particular embodiment, the computer system 100 includes a processor 105 connected to a bus which is connected to a main memory 115 (preferably implemented as RAM) and one or more internal data storage devices 110, such as a hard drive and/or other computer readable media having data recorded thereon. In some embodiments, the computer system 100 further includes one or more data retrieving device 118 for reading the data stored on the internal data storage devices 110.

[0140] The data retrieving device 118 may represent, for example, a floppy disk drive, a compact disk drive, a magnetic tape drive, etc. In some embodiments, the internal data storage device 110 is a removable computer readable medium such as a floppy disk, a compact disk, a magnetic tape, etc. containing control logic and/or data recorded thereon. The computer system 100 may advantageously include or be programmed by appropriate software for reading the control logic and/or the data from the data storage component once inserted in the data retrieving device.

[0141] The computer system 100 includes a display 120 which is used to display output to a computer user. It should also be noted that the computer system 100 can be linked to other computer systems 125 a-c in a network or wide area network to provide centralized access to the computer system 100.

[0142] Software for accessing and processing the nucleotide sequences of the nucleic acid codes ofSEQ ID Nos.1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 (such as search tools, compare tools, and modeling tools etc.) may reside in main memory 115 during execution.

[0143] In some embodiments, the computer system 100 may further comprise a sequence comparer for comparing the above-described nucleic acid codes of SEQ ID Nos. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 stored on a computer readable medium to reference nucleotide or polypeptide sequences stored on a computer readable medium. A “sequence comparer” refers to one or more programs which are implemented on the computer system 100 to compare a nucleotide sequence with other nucleotide sequences and/or compounds stored within the data storage means. For example, the sequence comparer may compare the nucleotide sequences of the nucleic acid codes of SEQ ID Nos. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 stored on a computer readable medium to reference sequences stored on a computer readable medium to identify homologies or structural motifs. Various sequence comparer programs identified elsewhere in this patent specification are particularly contemplated for use in this aspect of the invention. Protein and/or nucleic acid sequence homologies may be evaluated using any of the variety of sequence comparison algorithms and programs known in the art. Such algorithms and programs include, but are by no means limited to. TBLASTN, BLASTN, BLASTP, FASTA, TFASTA, and CLUSTALW (Pearson and Lipman, 1988, Proc. Natl. Acad. Sci. USA 85(8):2444-2448; Altschul et al., 1990, J. Mol. Biol. 215(3):403-410; Thompson et al., 1994, Nucleic Acids Res. 22(2):4673-4680; Higgins et al., 1996, Methods Enzymol. 266:383-402; Altschul et al., 1990, J. Mol. Biol. 215(3):403-410; Altschul et al., 1993, Nature Genetics 3:266-272).

[0144] In one embodiment, protein and nucleic acid sequence homologies are evaluated using the Basic Local Alignment Search Tool (“BLAST”) which is well known in the art (see, e.g., Karlin and Altschul, 1990, Proc. Natl. Acad. Sci. USA 87:2267-2268; Altschul et al., 1990, J Mol. Biol. 215:403-410; Altschul et al., 1993, Nature Genetics 3:266-272; Altschul et al, 1997, Nuc. Acids Res. 25:3389-3402). In particular, five specific BLAST programs are used to perform the following task:

[0145] (1) BLASTP and BLAST3 compare an amino acid query sequence against a protein sequence database;

[0146] (2) BLASTN compares a nucleotide query sequence against a nucleotide sequence database;

[0147] (3) BLASTX compares the six-frame conceptual translation products of a query nucleotide sequence (both strands) against a protein sequence database;

[0148] (4) TBLASTN compares a query protein sequence against a nucleotide sequence database translated in all six reading frames (both strands); and

[0149] (5) TBLASTX compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database.

[0150] The BLAST programs identify homologous sequences by identifying similar segments, which are referred to herein as “high-scoring segment pairs,” between a query amino or nucleic acid sequence and a test sequence which is preferably obtained from a protein or nucleic acid sequence database. High-scoring segment pairs are preferably identified (i.e., aligned) by means of a scoring matrix, many of which are known in the art. Preferably, the scoring matrix used is the BLOSUM62 matrix (Gonnet et al, 1992, Science 256:1443-1445. Henikoff and Henikoff, 1993, Proteins 17:49-61). Less preferably, the PAM or PAM250 matrices may also be used (see, e.g., Schwartz and Dayhoff, eds., 1978, Matrices for Detecting Distance Relationships: Atlas of Protein Sequence and Structure, Washington: National Biomedical Research Foundation). BLAST programs are accessible through the U.S. National Library of Medicine, e.g., at www.ncbi.nlm.nih.gov.

[0151] The BLAST programs evaluate the statistical significance of all high-scoring segment pairs identified, and preferably selects those segments which satisfy a user-specified threshold of significance, such as a user-specified percent homology. Preferably, the statistical significance of a high-scoring segment pair is evaluated using the statistical significance formula of Karlin (see, e.g., Karlin and Altschul, 1990, Proc. Natl. Acad. Sci. USA 87:2267-2268).

[0152] The parameters used with the above algorithms may be adapted depending on the sequence length and degree of homology studied. In some embodiments, the parameters may be the default parameters used by the algorithms in the absence of instructions from the user.

[0153]FIG. 4 is a flow diagram illustrating one embodiment of a process 200 for comparing a new nucleotide or protein sequence with a database of sequences in order to determine the homology levels between the new sequence and the sequences in the database. The database of sequences can be a private database stored within the computer system 100, or a public database such as GENBANK that is available through the Internet.

[0154] The process 200 begins at a start state 201 and then moves to a state 202 wherein the new sequence to be compared is stored to a memory in a computer system 100. As discussed above, the memory could be any type of memory, including RAM or an internal storage device.

[0155] The process 200 then moves to a state 204 wherein a database of sequences is opened for analysis and comparison. The process 200 then moves to a state 206 wherein the first sequence stored in the database is read into a memory on the computer. A comparison is then performed at a state 210 to determine if the first sequence is the same as the second sequence. It is important to note that this step is not limited to performing an exact comparison between the new sequence and the first sequence in the database. Well-known methods are known to those of skill in the art for comparing two nucleotide or protein sequences, even if they are not identical. For example, gaps can be introduced into one sequence in order to raise the homology level between the two tested sequences. The parameters that control whether gaps or other features are introduced into a sequence during comparison are normally entered by the user of the computer system.

[0156] Once a comparison of the two sequences has been performed at the state 210, a determination is made at a decision state 210 whether the two sequences are the same. Of course, the term “same” is not limited to sequences that are absolutely identical. Sequences that are within the homology parameters entered by the user will be marked as “same” in the process 200.

[0157] If a determination is made that the two sequences are the same, the process 200 moves to a state 214 wherein the name of the sequence from the database is displayed to the user. This state notifies the user that the sequence with the displayed name fulfills the homology constraints that were entered. Once the name of the stored sequence is displayed to the user, the process 200 moves to a decision state 218 wherein a determination is made whether more sequences exist in the database. If no more sequences exist in the database, then the process 200 terminates at an end state 220. However, if more sequences do exist in the database, then the process 200 moves to a state 224 wherein a pointer is moved to the next sequence in the database so that it can be compared to the new sequence. In this manner, the new sequence is aligned and compared with every sequence in the database.

[0158] It should be noted that if a determination had been made at the decision state 212 that the sequences were not homologous, then the process 200 would move immediately to the decision state 218 in order to determine if any other sequences were available in the database for comparison.

[0159] Accordingly, one aspect of the present invention is a computer system comprising a processor, a data storage device having stored thereon a nucleic acid code of SEQ ID Nos. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78, a data storage device having retrievably stored thereon reference nucleotide sequences or polypeptide sequences to be compared to the nucleic acid code of SEQ ID Nos.1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 and a sequence comparer for conducting the comparison. The sequence comparer may indicate a homology level between the sequences compared or identify structural motifs in the above described nucleic acid code of SEQ ID Nos. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 or it may identify structural motifs in sequences which are compared to these nucleic acid codes and polypeptide codes. In some embodiments, the data storage device may have stored thereon the sequences of at least 2, 5, 10, 15, 20, 25, 30 or 40 or more of the nucleic acid codes of SEQ ID Nos. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0160] Another aspect of the present invention is a method for determining the level of homology between a nucleic acid code of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 and a reference nucleotide sequence or polypeptide sequence, comprising the steps of reading the nucleic acid code or the polypeptide code and the reference nucleotide or polypeptide sequence through the use of a computer program which determines homology levels and determining homology between the nucleic acid code or polypeptide code and the reference nucleotide or polypeptide sequence with the computer program. The computer program may be any of a number of computer programs for determining homology levels, including those specifically enumerated herein, including BLAST2N or BLASTN with the default parameters or with any modified parameters. The method may be implemented using the computer systems described above. The method may also be performed by reading at least 2, 5, 10, 15, 20, 25, 30 or 40 or more of the above described nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 through use of the computer program and determining homology between the nucleic acid codes or polypeptide codes and reference nucleotide sequences or polypeptide sequences.

[0161]FIG. 5 is a flow diagram illustrating one embodiment of a process 250 in a computer for determining whether two sequences are homologous. The process 250 begins at a start state 252 and then moves to a state 254 wherein a first sequence to be compared is stored to a memory. The second sequence to be compared is then stored to a memory at a state 256. The process 250 then moves to a state 260 wherein the first character in the first sequence is read and then to a state 262 wherein the first character of the second sequence is read. It should be understood that if the sequence is a nucleotide sequence, then the character would normally be either A, T, C, G or U. If the sequence is a protein sequence, then it is preferably in the single letter amino acid code so that the first and sequence sequences can be easily compared.

[0162] A determination is then made at a decision state 264 whether the two characters are the same. If they are the same, then the process 250 moves to a state 268 wherein the next characters in the first and second sequences are read. A determination is then made whether the next characters are the same. If they are, then the process 250 continues this loop until two characters are not the same. If a determination is made that the next two characters are not the same, the process 250 moves to a decision state 274 to determine whether there are any more characters either sequence to read.

[0163] If there aren't any more characters to read, then the process 250 moves to a state 276 wherein the level of homology between the first and second sequences is displayed to the user. The level of homology is determined by calculating the proportion of characters between the sequences that were the same out of the total number of sequences in the first sequence. Thus, if every character in a first 100 nucleotide sequence aligned with a every character in a second sequence, the homology level would be 100%.

[0164] Alternatively, the computer program may be a computer program which compares the nucleotide sequences of the nucleic acid codes of the present invention, to reference nucleotide sequences in order to determine whether the nucleic acid code of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 differs from a reference nucleic acid sequence at one or more positions. Optionally such a program records the length and identity of inserted, deleted or substituted nucleotides with respect to the sequence of either the reference polynucleotide or the nucleic acid code of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77. In one embodiment, the computer program may be a program which determines whether the nucleotide sequences of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 contain a single nucleotide polymorphism (SNP) with respect to a reference nucleotide sequence.

[0165] Accordingly, another aspect of the present invention is a method for determining whether a nucleic acid code of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 differs at one or more nucleotides from a reference nucleotide sequence comprising the steps of reading the nucleic acid code and the reference nucleotide sequence through use of a computer program which identifies differences between nucleic acid sequences and identifying differences between the nucleic acid code and the reference nucleotide sequence with the computer program. In some embodiments, the computer program is a program which identifies single nucleotide polymorphisms. The method may be implemented by the computer systems described above and the method illustrated in FIG. 6. The method may also be performed by reading at least 2, 5, 10, 15, 20, 25, 30, or 40 of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and the reference nucleotide sequences through the use of the computer program and identifying differences between the nucleic acid codes and the reference nucleotide sequences with the computer program.

[0166] In other embodiments the computer based system may further comprise an identifier for identifying features within the nucleotide sequences of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0167] An “identifier” refers to one or more programs which identifies certain features within the above-described nucleotide sequences of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. In one embodiment, the identifier may comprise a program which identifies an open reading frame in the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77.

[0168]FIG. 7 is a flow diagram illustrating one embodiment of an identifier process 300 for detecting the presence of a feature in a sequence. The process 300 begins at a start state 302 and then moves to a state 304 wherein a first sequence that is to be checked for features is stored to a memory 115 in the computer system 100. The process 300 then moves to a state 306 wherein a database of sequence features is opened. Such a database would include a list of each feature's attributes along with the name of the feature. For example, a feature name could be “Initiation Codon” and the attribute would be “ATG”. Another example would be the feature name “TAATAA Box” and the feature attribute would be “TAATAA”. An example of such a database is produced by the University of Wisconsin Genetics Computer Group (www.gcg.com). Alternatively, the features may be structural polypeptide motifs such as alpha helices, beta sheets, or functional polypeptide motifs such as enzymatic active sites, helix-turn-helix motifs or other motifs known to those skilled in the art.

[0169] Once the database of features is opened at the state 306, the process 300 moves to a state 308 wherein the first feature is read from the database. A comparison of the attribute of the first feature with the first sequence is then made at a state 310. A determination is then made at a decision state 316 whether the attribute of the feature was found in the first sequence. If the attribute was found, then the process 300 moves to a state 318 wherein the name of the found feature is displayed to the user.

[0170] The process 300 then moves to a decision state 320 wherein a determination is made whether move features exist in the database. If no more features do exist, then the process 300 terminates at an end state 324. However, if more features do exist in the database, then the process 300 reads the next sequence feature at a state 326 and loops back to the state 310 wherein the attribute of the next feature is compared against the first sequence.

[0171] It should be noted, that if the feature attribute is not found in the first sequence at the decision state 316, the process 300 moves directly to the decision state 320 in order to determine if any more features exist in the database.

[0172] Accordingly, another aspect of the present invention is a method of identifying a feature within the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising reading the nucleic acid code(s) or polypeptide code(s) through the use of a computer program which identifies features therein and identifying features within the nucleic acid code(s) with the computer program. In one embodiment, computer program comprises a computer program which identifies open reading frames. The method may be performed by reading a single sequence or at least 2, 5, 10, 15, 20, 25, 30, or 40 of the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 through the use of the computer program and identifying features within the nucleic acid codes or polypeptide codes with the computer program.

[0173] The nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 may be stored and manipulated in a variety of data processor programs in a variety of formats. For example, the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 may be stored as text in a word processing file, such as MicrosoftWORD or WORDPERFECT or as an ASCII file in a variety of database programs familiar to those of skill in the art, such as DB2, SYBASE, or ORACLE. In addition, many computer programs and databases may be used as sequence comparers, identifiers, or sources of reference nucleotide sequences or polypeptide sequences to be compared to the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78. The following list is intended not to limit the invention but to provide guidance to programs and databases which are useful with the nucleic acid codes of SEQ ID NOs. 1, 2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 or the polypeptide codes of SEQ ID NOs. 6, 10, 14, 26, 28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78.

[0174] The programs and databases which may be used include, but are not limited to: MacPattern (EMBL), DiscoveryBase (Molecular Applications Group), GeneMine (Molecular Applications Group), Look (Molecular Applications Group), MacLook (Molecular Applications Group), BLAST and BLAST2 (NCBI), BLASTN and BLASTX (Altschul et al, J. Mol. Biol. 215: 403 (1990)), FASTA (Pearson and Lipman, Proc. Natl. Acad. Sci. USA, 85: 2444 (1988)), FASTDB (Brutlag et al. Comp. App. Biosci. 6:237-245, 1990), Catalyst (Molecular Simulations Inc.), Catalyst/SHAPE (Molecular Simulations Inc.), Cerius².DBAccess (Molecular Simulations Inc.), HypoGen (Molecular Simulations Inc.), Insight II, (Molecular Simulations Inc.), Discover (Molecular Simulations Inc.), CHARMm (Molecular Simulations Inc.), Felix (Molecular Simulations Inc.), DelPhi, (Molecular Simulations Inc.), QuanteMM, (Molecular Simulations Inc.), Homology (Molecular Simulations Inc.), Modeler (Molecular Simulations Inc.), ISIS (Molecular Simulations Inc.), Quanta/Protein Design (Molecular Simulations Inc.), WebLab (Molecular Simulations Inc.), WebLab Diversity Explorer (Molecular Simulations Inc.), Gene Explorer (Molecular Simulations Inc.), SeqFold (Molecular Simulations Inc.), the MDL Available Chemicals Directory database, the MDL Drug Data Report data base, the Comprehensive Medicinal Chemistry database, Derwents's World Drug Index database, the BioByteMasterFile database, the Genbank database, and the Genseqn database. Many other programs and data bases would be apparent to one of skill in the art given the present disclosure.

[0175] Motifs which may be detected using the above programs include sequences encoding leucine zippers, helix-turn-helix motifs, glycosylation sites, ubiquitination sites, alpha helices, and beta sheets, signal sequences encoding signal peptides which direct the secretion of the encoded proteins, sequences implicated in transcription regulation such as homeoboxes, acidic stretches, enzymatic active sites, substrate binding sites, and enzymatic cleavage sites.

[0176] The present invention will be further described with reference to the following examples; however, it is to be understood that the present invention is not limited to such examples.

[0177] In order to begin the physiological characterization of Cenarchaeum symbiosum, it was necessary to obtain enriched preparations of Cenarchaeum symbiosum for use in the construction of genomic DNA libraries in fosmid based vectors. Genomic DNA libraries were constructed from two enriched preparations using the methods described in Example 1 below.

EXAMPLE 1 Enrichment of Cenarchaeum symbiosum Cells in Samples Obtained from Axinella Mexicana

[0178] Enriched preparations of Cenarchaeum symbiosum for use in the preparation of the first fosmid genomic DNA library were obtained essentially as described in Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246, the disclosure of which is incorporated herein by reference. Briefly, a small individual of A. mexicana was incubated in calcium- and magnesium-free artificial seawater (ASW) containing 0.25 mg/ml Pronase. The tissue was then homogenized and enriched for archaeal cells by differential centrifugation.

[0179] Enriched preparations of Cenarchaeum symbiosum for use in preparing the second fosmid genomic DNA library were obtained from a different sponge individual using the following improved enrichment procedure. A small individual of A. mexicana was incubated in calcium- and magnesium-free artificial seawater (460 mm NaCl, 11 mM KCl, 7 mM Na₂SO₄, 2 mM NaHCO₃) containing 0.25 mg/ml Pronase at room temperature for one hour. The sponge tissue was rinsed in artificial seawater and homogenized in a blender. Large particles and spicules were removed by low-speed centrifugation (4000 rpm, Sorvall GSA rotor at 4° C.). The supernatant was next centrifuged at 5000 rpm for 5 min. at 4° C. to remove large sponge cells, and the resulting supernatant was centrifuged at 10,000 rpm in a GSA rotor at 4° C. for 20 min. to collect the Cenarchaeum symbiosum cells. Following centrifugation, the recovered cell fraction containing Cenarchaeum symbiosum was further incubated for 1 hr at 4° C. in 10 mM Tris/HCl pH 8 and 200 mM EDTA. The cells were then pelleted and subsequently purified on a 15% Percoll (Sigma) cushion in artificial sea water centrifuged at 2500 rpm in a Beckman SS34 rotor. Archaeal cells banded in the light, upper fraction after centrifugation. This cell fraction was washed in ASW and resuspended in TE buffer (10 mM TrisHCl pH 8, 0.1 mM EDTA). The additional incubation step was found to increase the lysis of sponge cells, which resulted in an enhanced separation of archaeal and eukaryotic cells in the percoll gradient.

[0180] Quantitative hybridization experiments were performed as described in DeLong, E. F. 1992. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89, 5685-5689, the disclosure of which is incorporated herein by reference, using an oligonucleotide specific for archaea having the sequence GTGCTCCCCCGCCAATTCCT (SEQ ID NO: 115). These hybridization experiments indicated that 25% to 30% of the total rRNA from this fraction was derived from archaea.

[0181] The enriched cell preparations were then utilized to construct fosmid libraries as described in Example 2 below.

EXAMPLE 2 Construction of Fosmid Libraries

[0182] DNA was extracted from the enriched preparations of Example 1 and inserted into fosmids as described in Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246 and Stein, J. L. et al. 1996. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J. Bacteriol. 178, 591-599, the disclosures of which are incorporated herein by reference. A vertical cross section of sponge (0.5 g) was mechanically dissociated in 0.22 μm filtered, autoclaved seawater using a tissue homogenizer. Cell lysis was accomplished by incubating the dissociated cells in 1 mg of lysozyme per ml for 30 min. at 37° C. followed by an incubation for 30 min. at 55° C. with 0.5 mg of proteinase K per ml and 1% SDS. The tubes were finally placed in a boiling water bath for 60 sec to complete lysis. The protein fraction was removed with two extractions with phenol:chloroform:isoamyl alcohol (50:49:1), pH 8.0, followed by a chloroform:isoamyl alcohol (24:1) extraction. Nucleic acids were ethanol-precipitated and resuspended in TE buffer (10 mM Tris.HCl/ mM Na₂-EDTA, pH 8.0). Approximately 5 μg of DNA was purified by CsCl equilibrium density gradiant ultracentriguation on a Beckman Optima tabletop ultracentrifuge using a TLA100 rotor.

[0183] The genomic DNA obtained above was inserted into fosmids as follows. The genomic DNA was partially digested with Sau3AI (Promega) and treated with heat-labile phosphatase (HK phosphatase; Epicentre). The partially digested genomic DNA was ligated with pFOS (See U. J. Kim et al., Nucleic Acids Res. 20:1083-1085 (1992), the disclosure of which is incorporated herein by reference) which had previously been digested with AatII, phosphatase treated (HK phosphatase), and subsequently digested with BamHI. The ligation mixture was used for in vitro packaging with the Gigapack XL packaging system (Stratagene) selecting for DNA inserts of 35 to 45 kb. The phage particles were transfected into E. coli DH10B (Bethesda Research LaboratoriesP and the cells were spread onto LB plates supplemented with 12.5 μg/ml chloramphenicol.

EXAMPLE 3 Identification of Fosmids Containing the Cenarchaeum symbiosum rRNA Operon

[0184] The fosmid libraries constructed above were screened to identify clones containing the rRNA operon. PCR reactions were conducted on the library using primers known to amplify the rRNA operon.

[0185] The first fosmid library yielded seven unique clones, out of a total of 10,236 recombinant fosmids, which contained the Cenarchaeum symbiosum rRNA operon. The second fosmid library yielded eight unique clones, out of a total of 2100 recombinant fosmids, which contained the Cenarchaeum symbiosum rRNA operon.

[0186] The sequences of the 16S rRNA genes in each of the 15 fosmids containing the Cenarchaeum symbiosum rRNA operon were determined. The sequences of the small subunit rRNA genes of these 15 fosmids exhibited variations with respect to one another. Ten of the fosmids contained a small subunit rRNA gene having the sequence of the 16S rRNA gene in the insert of SEQ ID NO: 1, while the remaining fosmids contained a small subunit rRNA gene having the sequence of the 16S rRNA gene in the insert of SEQ ID NO: 2. As discussed in more detail below, the differences in the sequences of the rRNA genes may be used to determine whether a sample contains Cenarchaeum symbiosum variant A or Cenarchaeum symbiosum variant B.

[0187] In addition to determining the sequences of the rRNA genes, the sequences adjacent to the rRNA genes were also determined.

EXAMPLE 4 Fosmid Sequencing

[0188] Partial restriction enzyme digests were conducted on two purified fosmids, fosmid 101G10 (which contains the variant A sequence) and fosmid 60A5 (which contains the variant B sequence). The partially digested DNA was used to construct plasmid libraries containing inserts of 1-2 kb. The resulting plasmids were sequenced using Applied Biosystems (ABI, Foster City, Calif.) Prism Dye-terminator FS reaction mix. Direct sequencing from fosmids was used for gap filling and resequencing to ensure accuracy. Fosmid sequencing was performed by using DNA from a single 3 ml overnight culture purified on an Autogen 740 automated plasmid isolation system. Each reaction consisted of one preparation of DNA directly resuspended by the addition of 16 μl H₂O, 8 μl oligonucleotide primer (1.4 pmol/μl) and 16 μl ABI Prism Dye-terminator FS reaction mix. Cycle sequencing was performed with a 96° C. 3 min. preincubation followed by 25 cycles of the sequence 96° C. sec./50° C. sec./60° C. 4 min. and a 5 min. post-cycling incubation at 60° C. Sequencing reaction products were analyzed on ABI 377 Prism Sequencers.

[0189] The complete sequences of the Cenarchaeum symbiosum derived inserts in the two fosmids are provided in the accompanying sequence listing as SEQ ID NO: 1 (fosmid 101G10) and SEQ ID NO: 2 (fosmid 60A5). The insert of fosmid 101G10 (SEQ ID NO: 1, designated variant A) was 32,998 bp and was syntenic over ca. 28 kbp with the 42,432 bp insert of fosmid 60A5 (SEQ ID NO:2, designated variant B). Analysis of the common 28 kbp region is shown in FIG. 1.

[0190] Although the sequences of both fosmids could be aligned unambiguously over most of the overlapping region, four large insertion/deletions ranging in size from 142 bp to 1994 bp were identified between positions 20,500 and 25,800. The longest insertion contained a repetitive element of 1784 bp, that was found in the sequence of SEQ ID NO: 1 between menA and ORF05. It was composed of a 3-fold direct repeat of 575 bp (rep1 through 3 in FIG. 1), with repeats exhibiting only minor sequence variation (95.8% to 98.7% identity).

[0191] A segment of 56 bp at the start of this repeat was also found adjacent to the 3′ terminus of the third direct repeat. No obvious structural or sequence similarities to known repeats or mobile genetic elements from other organisms were identified within the repeat sequence. Its occurrence in only one variant and its relatively low G+C content relative to the rest of the fragment suggest that it may have been acquired by horizontal transfer from a different genetic context.

[0192] The sequenced regions contained several open reading frames or RNA encoding sequences. Some of the identified open reading frames encode proteins having homology to previously identified proteins. In particular, some of the open reading frames encode proteins involved in several metabolic pathways, providing insight into the physiology of Cenarchaeum symbiosum.

[0193] An open reading frame which encodes a protein having homology to glutamate semialdehyde aminotransferase (a protein involved in heme biosynthesis) was identified between nucleotides 7604-8908 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 23558-24682 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 45 and 13 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 46 and 14 respectively in the accompanying sequence listing. A gene encoding glutamate semialdehyde aminotransferase has also been detected in a rRNA operon containing genomic fragment of a planktonic marine crenarchaeote. (Stein, J. L. et al. 1996. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J. Bacteriol. 178, 591-599)

[0194] An open reading frame encoding a protein having homology to triose-phosphate isomerase was identified between 13944-14612 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 29655-30491 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 57 and 25 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 58 and 26 respectively in the accompanying sequence listing. This triosephosphate isomerase represents the first such protein sequence reported in a crenarchaeote, and shares known archaeal signature sequences and deletions which distinguish archaeal triosephosphate isomerase genes from their eucaryal and eubacterial homologues.

[0195] An open reading frame encoding a protein having homology to the TATA binding protein was identified between 14616-15164 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 30501-31049 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 59 and 27 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 60 and 28 respectively in the companying sequence listing. This TATA box-binding protein (TBP) is similar to other known archaeal TBP's and is N-terminally truncated with respect to the eukaryal homologs. It shares 49% amino acid similarity with TBP from Pyrococcus woesii.

[0196] An open reading frame encoding a protein having homology to DNA polymerase (a protein involved in DNA replication and repair) was identified between nucleotides 15488-18025 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 31371-33905 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 61 and 29 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 62 and 30 respectively in the accompanying sequence listing.

[0197] The DNA polymerase of Cenarchaeum symbiosum has a high degree of similarity to the crenarchaeal homologs from the extreme thermophiles Sulfolobus acidocaldarius and Pyrodictium occultum (54% and 53% resp.) and exhibits all conserved motifs of B-(a-)type DNA polymerases and 3′-5′-exonuclease motifs, both indicative of archaeal polymerases. A more detailed phylogenetic analysis and biochemical characterization of the C. symbiosum polymerase has been published elsewhere. (Schleper, C., et al. 1997. Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum. J. Bact. 179, 7803-7811)

[0198] An open reading frame which encodes a protein having homology to dCMP deaminase (a protein involved in pyrimidine synthesis) was identified between nucleotides 18022-18663 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 33902-34456 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 63 and 31 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 64 and 32 respectively in the accompanying sequence listing.

[0199] An open reading frame encoding a protein having homology to the ATP dependent RNA helicase (a protein involved in translation) was identified between nucleotides 18638-20149 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 34559-36067 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 65 and 33 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 66 and 34 respectively in the accompanying sequence listing. The identified ATP RNA helicase is highly similar in sequence to homologues found in the genomic sequences of three euryarchaeota (Bult, C., et al. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273, 1058-1073; Klenk, H. P. et al. 1997. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fullgidus. Nature 390, 364-370; Smith, D. R.et al. 1997. Complete genome sequence of Methanobacterium thermoautotrophicum delta H: functional analysis and comparative genomics. J. Bacteriol. 179, 7135-7155).

[0200] An open reading frame encoding a protein having homology to MenA (a protein involved in menaquinone biosynthesis) was identified between nucleotides 20956-21834 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 37404-38282 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 71 and 37 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 72 and 38 respectively in the accompanying sequence listing.

[0201] An open reading frame encoding a protein having homology to the site specific DNA methyltranseferase proteins involved in restriction/modification was identified between nucleotides 26378-27454 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 40563-41669 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 75 and 41 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 76 and 42 respectively in the accompanying sequence listing.

[0202] An open reading frame encoding a protein having homology to the histone Hl DNA binding protein was identified between nucleotides 10625-1134 of the insert from fosmid 60A5 (SEQ ID NO: 2). This open reading frame has been assigned SEQ ID No: 5 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 6 in the accompanying sequence listing.

[0203] An open reading frame encoding a protein having homology to lysyl tRNA synthetase was identified between nucleotides 13046-14620 of the insert from fosmid 60A5 (SEQ ID NO: 2). This open reading frame has been assigned SEQ ID No: 9 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 10 in the accompanying sequence listing.

[0204] A hypothetical open reading frame was identified between nucleotides 11478-13046 of the insert from fosmid 60A5 (SEQ ID NO: 2). This open reading frame has been assigned SEQ ID No: 7 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 8 in the accompanying sequence listing.

[0205] An open reading frame encoding a protein having homology to peptidylprolyl cis/trans isomerase (a chaperone) was identified between nucleotides 20156-20434 of the insert from fosmid 101G10 (SEQ ID NO: 1) on the strand complementary to that provided in the sequence listing. This open reading frame has been assigned SEQ ID No: 67 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 68 in the accompanying sequence listing.

[0206] An open reading frame encoding a protein having homology to glucose-1-dehydrogenase was identified between nucleotides 28065-29843 of the insert from fosmid 101G10 (SEQ ID NO: 1). This open reading frame has been assigned SEQ ID No: 79 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 80 in the accompanying sequence listing.

[0207] A hypothetical open reading frame designated Hypothetical 01 was identified between nucleotides 1358-2290 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 17329-18213 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 43 and 11 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 44 and 12 respectively in the accompanying sequence listing.

[0208] A hypothetical open reading frame designated Hypothetical 02 was identified between nucleotides 8961-9767 of the insert from fosmid 101G10 (SEQ ID NO: 1) between nucleotides 24913-25728 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 47 and 15 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 48 and 16 respectively in the accompanying sequence listing.

[0209] An open reading frame designated ORF 01 was identified between nucleotides 9772-10479 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 25732-26427 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 49 and 17 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 50 and 18 respectively in the accompanying sequence listing.

[0210] An open reading frame designated ORF 02 was identified between nucleotides 10545-10922 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 26504-26881 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 51 and 19 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 52 and 20 respectively in the accompanying sequence listing.

[0211] An open reading frame designated ORF 03 was identified between nucleotides 11382-11987 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 27337-27936 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 53 and 21 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 54 and 22 respectively in the accompanying sequence listing.

[0212] An open reading frame designated ORF 04 was identified between nucleotides 12916-13737 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 28822-29631 of the insert from fosmid 60A5 (SEQ ID NO: 2) on the strands complementary to the insert strands provided in SEQ ID NOs: 1 and 2. These open reading frames have been assigned SEQ ID NOs: 55 and 23 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 56 and 24 respectively in the accompanying sequence listing.

[0213] An open reading frame designated Hypothetical 03 was identified between nucleotides 20554-20955 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 37002-37403 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 69 and 35 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 70 and 36 respectively in the accompanying sequence listing.

[0214] An open reading frame designated ORF 05 was identified between nucleotides 25151-26377 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 39454-40572 of the insert from fosmid 60A5 (SEQ ID NO: 2). These open reading frames have been assigned SEQ ID NOs: 73 and 39 respectively in the accompanying sequence listing, while the polypeptides they encode have been assigned SEQ ID NOs: 74 and 40 respectively in the accompanying sequence listing.

[0215] An open reading frame encoding a protein with no homology to known proteins was identified between nucleotides 3-10421 of the insert from fosmid 60A5 (SEQ ID NO: 2). This open reading frame has been assigned SEQ ID No: 3 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 4 in the accompanying sequence listing.

[0216] An open reading frame designated ORF06 was identified between nucleotides 27535-28002 of the insert from fosmid 101G10 (SEQ ID NO: 1). This open reading frame has been assigned SEQ ID No: 77 in the accompanying sequence listing, while the polypeptide it encodes has been assigned SEQ ID No: 78 in the accompanying sequence listing.

[0217] A gene coding for tRNA^(TYr) was identified between nucleotides 12129-12251 of the insert from fosmid 101G10 (SEQ ID NO: 1) and between nucleotides 28058-28180 of the insert from fosmid 60A5 (SEQ ID NO:2). This tRNA contains a 45 bp intron in the vicinity of the anticodon loop.

[0218] Table 1 shows the level of homology between the open reading frames in the inserts from fosmid 101G10 and fosmid 60A5 at the nucleic acid level. Table 1 also shows the level of homology at the amino acid level between the polypeptides encoded by the insert from fosmid 101G10 and fosmid 60A5. Nucleic acid homology was calculated using BLASTN with the default parameters. Amino acid homology was calculated using FASTA with the parameters. As shown in Table 1 and FIG. 1, the protein coding regions were highly similar in both nucleic acid and deduced amino acid sequences.

[0219] Over the 28 kb common region in the 101G10 and 60A5 inserts, the inserts shared >99.2% identity in their ribosomal RNA genes, approximately 87.8% overall DNA identity, an average of 91.6% similarity in ORF amino acid sequence, and complete colinearity of protein encoding regions. As shown in Table 1, in protein coding regions the DNA identity of the two contigs ranged from 80.9% (triose phosphate isomerase) to 91.5% (Hypothetical 03). Within intergenic regions the identity dropped to 70-86%, and small insertions or deletions were found frequently. The high similarity in coding regions and upstream sequences aided in the identification of genes, start codons, and putative transcriptional promoter motifs (see below). Genes appear as densely packed in C. symbiosum as they are in other sequenced archaeal genomes (Bult, C., et al. 1996. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273, 1058-1073, Klenk, H. P. et al 1997. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature 390, 364-370; Smith, D. R., et al. 1997. Complete genome sequence of Methanobacterium thermoautotrophicum delta H: functional analysis and comparative genomics. J. Bacteriol. 179, 7135-7155).

[0220] The ribosomal RNA operon of Cenarchaeum symbiosum is composed of the genes for the 16S and 23S rRNAs separated by a spacer of 131 bp. This organization is typical of crenarchaeotes, and differs from rRNA operons of euryarchaeotes, which usually contain 5S RNA and tRNA genes. (Garrett, R. A. et al. 1991. Archaeal rRNA operons. TIBS 16, 22-26). The large subunit rRNA genes are located between nucleotides 2680-5674 of SEQ ID NO: 1 (fosmid 101G10) and between nucleotides 18645-21639 of SEQ ID NO: 2 (fosmid 60A5). The small subunit rRNA genes are located between nucleotides 5806-7278 of SEQ ID NO: 1 (on the opposite strand from that shown in the Sequence Listing, as indicated in FIG. 1) and between nucleotides 21771-23243 of SEQ ID NO: 2. The large and small subunit rRNA genes in the two fosmids were 99.2% and 99.3% identical, respectively.

[0221] As mentioned above, the sequences of the Cenarchaeum symbiosum derived inserts in fosmids 101G10 and 60A5 had a high degree of homology but were not completely identical. The sequence of the insert in fosmid 101G10 was designated variant A, while the sequence of the insert in fosmid 60A5 was designated variant B. Such sequence differences could arise if the fosmid inserts were derived from two closely related but distinct strains of Cenarchaeum symbiosum or, alternatively, the sequence differences could be due to cloning or sequencing artifacts. To confirm that the fosmid inserts were in fact derived from two closely related strains, portions of the inserts in a plurality of different fosmids were sequenced to determine whether they were identical to either of the inserts in fosmids 101G10 and 60A5, as would be the case if there were in fact two closely related strains of Cenarchaeum symbiosum.

[0222] In particular, the ribosomal RNA spacer regions of variant A and variant B contained 10 distinguishing signature nucleotides and the 16S rRNA genes of variant A and variant B contained two distinguishing nucleotides. Example 5 provides the results of a PCR based analysis of the 16S rRNA gene and the 16S-23S spacer region in 13 different fosmid inserts.

EXAMPLE 5 PCR Based Analysis of Fosmid Inserts to Determine Whether They Contain the Variant A or Variant B Sequences

[0223] Primers 21F and 459R-LSU (CTTTCCCTCACGGTA, SEQ ID NO: 116) were used to amplify the 16S-23S spacer region from the fosmids. The amplification products were sequenced using primer SP23rev (CTA TTG CCG TCT TTA CACC, SEQ ID NO: 11 7).

[0224] PCR reactions with two archaea-specific 16S rDNA primers (21F and 958R (DeLong, E. F. 1992. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89, 5685-5689, the disclosure of which is incorporated herein by reference), one of which was biotinylated, were used to amplify a 950 base pair (bp) fragment from the fosmids. The PCR products were purified and sequenced as described in Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246 with primer 519R 16S rDNA

[0225] The results of this analysis are shown in Table 2. As shown in Table 2, in samples obtained from several unique rRNA operon-containing fosmids, a sequence identical to either variant A (101G10) or variant B (60A5) was present.

[0226] The above methods may also be used to determine whether a biological sample contains variant A and/or variant B. In such procedures, nucleic acids are obtained from the biological sample, amplified using the above primers, and sequenced using the above oligonucleotide to determine whether the sample contains the variant A and/or the variant B sequence.

[0227] Similarly, the amplification reaction may be conducted using any primers which generate amplification products having sequences which differ between variant A and variant B. The amplification products may then be sequenced to determine whether they have the sequence of variant A and/or variant B. In some embodiment, the amplification reaction may be conducted under conditions in which the amplification primers specifically hybridize to one of the variants.

[0228] RFLP analyses were also be used to assess whether the fosmids contained the sequence of variant A or variant B as described in Example 6 below.

EXAMPLE 6 RFLP Based Analysis of Fosmids to Determine Whether They Contain the Variant A or Variant B Sequences

[0229] Primer set 21F (DeLong, E. F. 1992. Archaea in coastal marine environments. Proc. Natl. Acad Sci. 89, 5685-5689) and 459R-LSU for the amplification of 2.2 kbp of the ribosomal operon, primer set GSAT810F (GAATCCGCC CCCGACTATCTT, SEQ ID NO: 118) and 16S37REV (CATGGCTTAGTATCAATC SEQ ID NO: 119) for the amplification of the 16S RNA-GSAT region (2.2 kbp) and primer set Cenpol357F (ACITACAACGGI GACGAYTTTGA SEQ ID NO: 120) and Cenpol735R (CACCCCGAARTAGTTYTTYTT SEQ ID NO: 121) for an internal DNA polymerase fragment (of 1134 bp) were used in PCR reactions with 5 ng of purified fosmids. The PCR products were cut with TaqI and HpaII (16S-23S RNA), HaeIII and RsaI (GSAT-16S RNA) or HaeIII and AvaII (polymerase) and analyzed on 2% agarose gels.

[0230] The results are shown in Table 2. If the pattern did not exactly match but closely resembled the RFLP of either type A or B, it was assigned as a lower case letter (a or b, Table 2), meaning that at least 3 out of 4 or 3 out of 5 bands created by restriction digest appear identical in size to the ones from either type A or B. As shown in Table 2, RFLP patterns of the 1150 bp fragment covering the 5′-end of the GSAT gene and 16S gene and the internal fragment of 1134 bp from the DNA polymerase gene revealed that all fosmids analyzed could again be assigned to either the A or B type, although slight variations were also detected (lower case letters in Table 2), suggesting that both variants exhibit further microheterogeneity which is detectable in protein coding and intergenic regions.

[0231] The above methods may also be used to determine whether a biological sample contains variant A and/or variant B. In such procedures, nucleic acids are obtained from the biological sample, amplified using the above primers, and digested as described above to determine whether the sample contains the variant A and/or the variant B sequence. Similar analyses may also be performed using other portions of the sequences of SEQ ID NOs: 1 and 2 which are different from one another.

[0232] To further confirm the existence of two closely related strains of Cenarchaeum symbiosum, biological samples were obtained from several individual sponges and analyzed to determine whether the samples contained variant A and/or variant B. Example 7 below provides the results of a PCR analysis of the Cenarchaeum symbiosum 16S rRNA genes in samples obtained from several individual sponges in different locations and at different times.

EXAMPLE 7 Analysis of Samples from Individual Sponges

[0233] The 16S rRNA genes of variant A and variant B differ at positions 175 and 183.7 (E. coli numbering). PCR reactions with two archaea-specific 16S rDNA primers (21F and 958R (DeLong, E. F. 1992. Archaea in coastal marine environments. Proc. Natl. Acad. Sci. 89, 5685-5689, the disclosure of which is incorporated herein by reference), one of which was biotinylated, were used to amplify a 950 base pair (bp) fragment from total nucleic acids derived from several different sponge individuals. The PCR products were purified and sequenced as described in Preston, C. M. et al. 1996. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov. Proc. Natl. Acad. Sci. USA 93, 6241-6246 with primer 519R, the disclosure of which is incorporated herein by reference.

[0234] The amplification products were sequenced to determine whether they corresponded to variant A and/or variant B. The results are shown in Table 3. As shown in Table 3, in 15 out of 16 cases U/C ambiguities were found at the signature positions, indicating the presence of both variants in samples obtained from a single sponge (Table 3). Only one sponge (S4) yielded an unambiguous sequence identical to variant A, but variant B was detected in this individual by another criterion (see below).

[0235] Hybridization analyses were also used to determine whether individual sponges harbored variant A and/or variant B. The results of these analyses are provided in Example 8 below.

EXAMPLE 8 Hybridization Based Analysis of Samples Obtained from Axinella Mexicana to Determine Whether the Samples Contain Variant A and/or Variant B

[0236] Two oligonucleotides specific for each variant type were designed from the 23S rDNA gene sequences of fosmids 101G10 and 60A5. The probes differed in 3 positions and have the sequences ACACTTCAACTATTTCCTG (SEQ ID NO: 122 variant A) and ACACTTTGACTATTTCGTG (SEQ ID NO: 123, variant B). Nucleic acid samples from individual sponges (300 ng) and controls (fosmids 101G10 and 60A5, 50 ng each) were denatured, bound to nylon membranes (Hybond-N, Amersham), hybridized with the labeled probes (Massana, R. et al. 1997. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Appl. Env. Microb. 63, 50-56, the disclosure of which is incorporated herein by reference in its entirety) and washed at 41.5° C. Hybridization was analyzed by autoradiography.

[0237] The results are provided in Table 3. In the samples from the majority of host sponges examined, the presence of both 23S rRNA variants was observed, confirming that the specific association of C. symbiosum with its host typically involves the presence of both variants.

[0238] The data provide strong evidence that these genomic clones are derived from two very closely related, but distinct strains, as opposed to representing two ribosomal RNA operon regions originating from the same organism. This conclusion is consistent with the observation that all crenarchaeota characterized to date contain only one ribosomal RNA operon (Garrett, R. A. et al. 1991. Archaeal rRNA operons. TIBS 16, 22-26).

[0239] The high conservation between the inserts in fosmid 101G10 and fosmid 60A5 was not entirely confined to coding regions but also extended into adjacent upstream sequences. Due to this upstream similarity, and also because the average G+C content of the sequences was relatively high, it was possible to readily identify prospective transcriptional (A+T rich) promoter elements. A motif corresponding to the consensus of the archaeal TATA-box-like element (C/T-T-T-A-T/A-A) (Hain, J. et al. 1992. Elements of an archaeal promoter defined by mutational analysis. Nucl. Acids. Res. 20, 5423-5428) was identified upstream of nearly all genes (FIG. 2). The exceptions were the genes encoding MenA and DNA polymerase which are located immediately downstream of other ORFs and may therefore be transcribed as polycistronic mRNAs. In vivo and in vitro studies in other archaea have shown that initiation of transcription occurs consistently 24 to 28 bp downstream from the central T of this motif (Hain, J et al. 1992. Elements of an archaeal promoter defined by mutational analysis. Nucl Acids. Res. 20, 5423-5428; Palmer, J. R. and Daniels, C. J. 1995. In vivo definition of an archaeal promoter. J. Bacteriol. 177 1844-1849). For twelve of the protein encoding genes, the promoter element was found 25 to 30 bp upstream of the ORF (FIG. 2), suggesting that transcriptional initiation occurs in close proximity to, or directly at, the translational start codon.

[0240] A similar observation has been made for 30 of the predicted 100 strong and medium promoters from 156 kbp sequence of Sulfolobus solfataricus (Sensen, C. W. et al. 1996. Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2. Molec. Microb. 22, 175-191). Transcription initiation at, or in close proximity to, the translational start codons has been mapped for some genes in Halobacterium salinarium (Brown, J. W. et al. 1989. Gene structure, organization, and expression in archaebacteria. CRC Crit. Rev. Microb. 16, 287-337) and S. solfataricus (Klenk, H. P., et al. 1993. Nucleotide sequence, transcription and phylogeny of the gene encoding the superoxide dismutase of Sulfolobus acidocaldarius. Biochim. Biophys. Acta 1174 95-98), and alternative mechanisms for initial mRNA-ribosome contact in Archaea have been hypothesized (Brown, J. W. et al. 1989. Gene structure, organization, and expression in archaebacteria. CRC Crit. Rev. Microb. 16, 287-337).

[0241] The promoters listed in FIG. 2, or fragments thereof, may be used in expression vectors or expression systems. In one embodiment, the promoters listed in FIG. 2 may be operably linked to coding regions and introduced into archaebacteria, and in particular Cenarchaeum symbiosum, to express the encoded gene product in the archaebacterial cells.

[0242] Alternatively, the promoters listed in FIG. 2 may be operably linked to coding regions and introduced into host cells which are not normally capable of directing transcription from archaebacterial promoters. In addition, genes encoding the proteins required for transcription from these promoters are also introduced into the host cells. The genes encoding these transcription factors may be on the same vector as the promoter from Cenarchaeum symbiosum or on a different vector. In some embodiments, the genes encoding these transcription factors are linked to an inducible promoter. Expression of the transcription factors is induced when it is desired to express the proteins which are operably linked to the promoter from Cenarchaeum symbiosum.

[0243] Although this invention has been described in terms of certain preferred embodiments, other embodiments which will be apparent to those of ordinary skill in the art in view of the disclosure herein are also within the scope of this invention. Accordingly, the scope of the invention is intended to be defined only by reference to the appended claims. All documents cited herein are incorporated herein by reference in their entirety. TABLE 1 Comparison of Overlapping Coding Sequences from Fosmid 101G10 and Fosmid 60A5 Gene Functional % Identity Name¹ Category Nucleotide Amino Acid Hypothetical 01 unknown 81.4 76.6 23S translation 99.16 16S translation 99.3 GSAT heme biosynthesis 83.2 83.8 Hypothetical 02 unknown 83.4 81.4 ORF 01 unknown 83.3 85.7 ORF 02 unknown 89.9 95.2 ORF 03 unknown 87.9 86.7 tRNA^(tyr) translation 99.2 ORF 04 unknown 87.8 88.1 TIM glycolysis 80.9 83.3 TBP transcription 83.4 86.3 DNA polymerase replication/repair 89.0 93.9 dCMP deaminase pyrimidine synthesis 85.7 89.8 RNA helicase (ATP translation 86.1 92.2 dependent) PPI chaperone 88.4 92.5 Hypothetical 03 unknown 91.5 92.4 MenA menaquinone 86 89.4 biosynthesis ORF 05 unknown 87.5 90.6 Methylase restriction/modification 86.4 87.5

[0244] TABLE 2 Analysis of Polymorphism at Four Distinct Loci in Different Fosmids 16S-23S 16S-GSAT*³ DNA Pol*³ Fosmid 16S RNA*¹ spacer*² HaeIII RsaI HaeIII AvaII 101G10 A A A A A A 60A5 B B B B B B 15A5 B B — — b b 43H4 A — — — A A 60H6 A A — — a/b B 69H2 A — — — A A 87F4 B — — — b a/b C1H5 A A A A C4H1 A A A A C4H9 A A A A A B C7D4 A A A A A A C8B8 B B B B B b C15A3 A A A A C17D2 B — b B B b C20B5 A A a a/b

[0245] TABLE 3 Detection of C. symbiosium Variants in Natural Populations of A. mexicana Variations in 23S rRNA Variation in 16S Hybridization A. mexicana Individual rDNA Positions** Variant or Isolated DNA Source* 175 183.7 Type A Variant Type B fosmid 101G10 from s12 U U + − fosmid 60A5 from s12 C C − + s12 Y Y + + s1 — — + + s2 — — + + s3 Y Y + + s4 U U + w s5 Y Y — — s6 Y Y + + s7 — — + w s8 Y Y + + s9 Y Y + w s10 — — + + s11 Y Y + + s13 — — + + s14 — — + w s16 — — + + s17 — — − w s18 Y Y − w s19 — — + + s20 — — + + s21 — — + + s22 — — + + s23 — — + + s24 — — + + s25 — — + + s26 — — + + s27 — — + + s28 — — + + s29 — — + + s30 — — + + hs1 — — + + hs2 — — + + hs3 Y Y + w hs4 Y Y + w hs5 Y Y + + hh1 — — w w hh2 Y Y + + hh3 Y Y + + Aq1 Y Y — — Aq2 Y Y — — Aq3 — — + +

[0246]

0 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 123 <210> SEQ ID NO 1 <211> LENGTH: 32998 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (7604)...(8908) <221> NAME/KEY: CDS <222> LOCATION: (8961)...(9767) <221> NAME/KEY: CDS <222> LOCATION: (10545)...(10922) <221> NAME/KEY: CDS <222> LOCATION: (13944)...(14612) <221> NAME/KEY: CDS <222> LOCATION: (18638)...(20149) <221> NAME/KEY: CDS <222> LOCATION: (20554)...(20955) <221> NAME/KEY: CDS <222> LOCATION: (20956)...(21834) <221> NAME/KEY: CDS <222> LOCATION: (25151)...(26377) <221> NAME/KEY: CDS <222> LOCATION: (27535)...(28002) <221> NAME/KEY: CDS <222> LOCATION: (28065)...(29483) <400> SEQUENCE: 1 gatccttgac ctctgcgctt attgcagcca tggactgacc ggccgtgcgg ggctaaataa 60 agctgaggcg ccgcctgcag gctctgctca gccgttgatt atacagtact cgcactcgca 120 ggtcttgctt tcgtcatctt ttgccctgca cttggcgtgc tggcccgact ggcactggac 180 gcagatctcg tctatgttgt ctatcgaggt gtactttttg gactggtcaa agccgaagcc 240 gccgtccttt ggtccaacca tgaagcggtc agggccgtta tgccttaatt atctttgccg 300 tcggggcggg gccgcttctg ccggcgggag ccccccttga ggccgctccc cggctcgttg 360 tcccacaggt gcatgctgcc cctgatcata aacgagccga ctatgattgc tacaagcccg 420 cccactatca gtataaacag cctagccacg ccccccattc tgcccatgcg tgtaatatgc 480 tgcacctgta aacaaacatt gcgcggggca tgggccgtcc ggacagacag aactgcccat 540 gagacaggtg cctgcgggcc ggtaagctac attaatttat caccccccac gggcgggccc 600 catgagcagg accaagagaa taatcatctg ggcatccata ctgggcgggg ggataatcta 660 ctttctcgtc cagggcgaga ttgccagaaa tgtattgtcc tgagaacaga agccatgcag 720 caactgcccg atcgtttttc aggtacctac tgggcttttt ggagttcgtt gatcaaaagc 780 ggtacaggta ttctatacat gccagtcttg gctggaaaaa taaattgaag atcggcggat 840 cctatctacg agcgcctgcc tgcttttcac ttgactagcc ggagtacttc gtctgcaatt 900 tctgtatagc taggcattta tgtagttgag atacatgtcc gcgggatccg tttcatagtc 960 tgaatcaaac accggatcat tccttctctt taattcctta aatgcctgat gcagttcaag 1020 caggacggtt acatcatcgc gtttgattat ccgttctgtt gtttcagctt tttgctcatt 1080 ctcatccatg attataggct acggtatttg actaaaaagg tttccatctt catgagtcgt 1140 gtgtttgccg tggaattacc taccggggga acataaaaaa atgagtcata aacagcgcac 1200 tgcatccacc gtggacccat gagacaacga gccggcagcg gtgcacagca ccgagtacaa 1260 ccccgcaatc gtcttatcca acgacctgcc ctatgaaaac tccagacgga tctcttccgg 1320 gcatccagta cccatgtatg tgagaattct gactatctta ccgtggtgtt ggcagtgtct 1380 caacgacggt attaccgagg tgattcatga taattttgtt gactggagta tactgaaaca 1440 tggtatttgc cgcatgaaat actgacagca ccagatcttg aaattcttgt tcatcttcca 1500 gatcagttac ttttaagcca atccttttac attcttctct cgatatgcgc cttccatgac 1560 tgtgatattt tttagaggaa gacattattt ctgatatttt ttttgacttt tttaccgctg 1620 cagactcgtt ctcaaacatg tatctagcca gccatttttg tacaagttcc acacttagct 1680 tctggctgct aatgcatttt tgaattagcc cgggaggata ttgccctaac agtggaagcc 1740 atgcgccgag cctccccggg tgtttttctg acaccgactg tgcttcttga aactcgctaa 1800 ttagaaactg tgcagacatt atgtgcatgc cggtcctggt tggaataata aattgggggt 1860 cggtgggacc tatcgatgag tgtttaccca ttaccaggca attcgatgag catgcaagca 1920 tcgcagctgc cgacatcgcg gcatatggga taatgatccg gacattttta aattttgcac 1980 gtatgtatga gacgattgct tcggtggact cgacggagcc ccccggactg tggagtatta 2040 aatctaattt cttagtcttt aaatcacgca tcatcctcat aaatccatac aggtcaccat 2100 ttgttatgag agcttcatta gacgtatgcg cttcgtccgt tatccagttg gtcgcataca 2160 gtattgtatc cctccccgaa tactgttgca attttgaaag atagtcgtga agtactacac 2220 caggtgcctg ctcaccgact gactccatta gtcgaagtac gtcgtttgct atttctgcgt 2280 aactaggcat ttatgtagtt gaaatgacta cccgcgggaa tcataccata gtctgtgtcg 2340 tatgacttgc cttttttctt catcaatttc tcatattcct catgcagttc gagcaggatg 2400 gccatgtcac tacgtttgtt cgtctgtttt gttgtctcgg gcttttggtc catattatta 2460 tccatgctag taaaggacta tgttccttta aaaaggttcg tgattttaat ttccaagtgt 2520 ttgcctcgca atttcctcca aggcacatga aaaacgggcc acaggcagag cacagcatcc 2580 gctggggacc catgaaataa gcccccggcg gtgcacagca tccgctgggg gctcaataaa 2640 aaaatgagtc atcatgcata gtctctatgt aaatggctga accggtgttt tggtcgatta 2700 gtaaaggctg gctcaccact cgccgaagct tgtgggatac accaccttcc tatcaacgca 2760 gtcttcttct gcgaaccttc atccgaagaa ggaatatctt gtctcgggat aggattcgtg 2820 cttagatgct ttcagcactt agcctagatg gcttagctgc ccggcctgcc ctgtcggaca 2880 accggtagac cagtggccac gcctctctgt tcctctcgta ctaagagcga cttcccctca 2940 gatattcgcg cttccatcag gcagaggccg acctgtctca cgacggtcta aacccagctc 3000 atgttccctt ttaataggcg agcagcctca cccttggccc ctgctgcagg accaggatag 3060 gaaaagccga catcgaggta ccaaaccgcg gggtcgatag gagctctcgc ccgcgacgag 3120 cctgttatcc ctggggtaat ttttctgtca cctccgggcc ccaatagtgg gcacacgaag 3180 gatcgctaag ccagactttc gtctatgaat tccgtgcgtt tggaaatcca ttcagtctag 3240 tttttggctt tgccctcttc agcggatttc tgacccgctt gaactaaact ttgggcccct 3300 ttgatatctt ttcaaagggg tgccgcccca gccgaactgc ccacctgcac atgtccccgg 3360 tcttcaccgg gtaagtggca ctgcaggaaa tgtctggtgt tacatcggcg tcccctgacg 3420 tcccaaagaa cgccaggaaa tgactcccag atacgctatg cactccctgc tataccacaa 3480 gcacaagctg cagtaaaact ccacggggtc ttctctcccc gatggaagat gatggactgt 3540 tcgtccacct tatgtggctt caccgggttg taggcgggga cagtggggct ctcgttgttc 3600 cattcatgca cgtcggaact tacccgacaa ggcatttggc taccttaaga gagtcagagt 3660 tactcccggc gttaaccggt ccttagctcg gttgaaccca agttttagat accggcaccg 3720 gccaggattc agcgactata catacccttt cgggctagca gtcgcctgtg tttttattaa 3780 acagtcgaaa cccccttgtc actgcaacct gctgccgcca ttcctcatga cagctgcagg 3840 catcccttat acctaagcta caggactaat ttgccgaatt ccctcgccat acggtatacc 3900 cgtagcacct tagtttacta aaccagcgca cctgtgtcgg atctgggtac gaacttgcag 3960 tttgctagcc gcacggtctt tcatggtctc ctggaatcgg gaaaactctg ctaacgcaaa 4020 gccactcccg cctcgggcct gttctcgtca ttacgacact cccaggccct cgaacggttc 4080 gacacgacga cggtcatgtt ccccctatcc ggaagcgaac catgcggttc aaacgctccc 4140 tgcaaggtac cagaatatta actggtttcc cattcggact actctgttga ggcagtcctt 4200 aggatcgact aactccaggc tgacgacgca ttgcctggaa acccttgcgc ttacggtggt 4260 gcggattctc accgcactat gctgttactg ccaccaggat ctgcaataga aatcggtcca 4320 caggacgtca ccgccctgct tcgtcccaat cactacgcca acctaccacg gtgcacctat 4380 cacggtgcac gtctggagta tcggtactct gctttagccc cgtccgtttt tgtggcgccc 4440 tcgctcggca ggtaagttgt tacacacttt ttgaaggata gctacttctg agcttacctc 4500 cctgctgtct tggcgatgac acgcactttg gcttgacact tagcagaaat ttggggacct 4560 taactccagt ctgggttaaa cccctctcgg tcgtgaacct tacgtcacac gaacccgtgt 4620 ccatgcttct gcgatgtgta tccgttcgga gtttgaatgg atggtgagga atctcttccc 4680 cgcgccaccc tatcagtgct ctaccggaaa caccatctcc acatagcacg ccctgcgaga 4740 cgcttcggtt ggaactagca agcgccagtc tagattggtt tttgacccct attcccaagt 4800 cacacaaacg agttgcacgt cagaactgct gcagacctcc agtgggcttt cgcccacctt 4860 catcttgctc aggaatagat cgactggctt ctagccttac cgccatgact taacgcactt 4920 tcacacgctt ctcctcacaa tgctgcgaga attcggtttc ccttcggcta cgcctttcta 4980 ggcttaacct cgccatgaca gcaagctccc tggcccgtgt ttcgagacgg aacgcatgac 5040 actgacgaca tgagctccgg actttcagct ccattgctgg aacctccggt ccgaaaaaat 5100 cgtctttcat gccatgcacg tctgtaagca ataggtttca tgcacttttc accccccttc 5160 cggggtactt ttcagctttc cctcacggta ctagtacact atcggtcttg agagatattt 5220 agcctttgat gctactttca ccaatcttcg ctgcccactg ccaaggacaa ctactcgggt 5280 gctggccctg ccccattcca cttcgtctag gggggtatca ccctctaagc cggaacattt 5340 cagaacactt caactatttc ctggggccat tgcgccgcac caaaacacca catctcggcc 5400 gcgttaccgc ggcagattca gtttgggctc tttccttttc gatcgcctct acttgggaaa 5460 tctctattga tttctcttcc tcgtggtact aagatgcttc aattcccacg gttcgacctc 5520 cgcttgcgcg gagtatacag gattcctatt cggaaatctc gggatcaacg ggtgcgtgca 5580 cctaccccga gcttatcgca gcttgccacg tccttcttct ctcctcaagc ctagcaatcc 5640 tcctattgcc gtctttacac cggcatattc agccacatat tacacgacta tgcatgatga 5700 tcatcgcagt ccccagggga gggggccgct acatccttca tacaccactt gcgtggtgca 5760 ttgcaccatg caaagatcat gtgcattctg ttcaaaccag tttctaagga ggtgatccga 5820 ccgcaggttc ccctacggtc accttgttac gacttttccc ttgtcgctta cctcaagttc 5880 gataacgcca attagacgtc acctcactaa aagcaaactt caatgaaacg acgggcggtg 5940 tgtgcaagga gcagggacgt attcactgcg cggtaatgac gcgcggttac tagggattcc 6000 agattcgtga gggcgagttg cagccctcag tcataactgt ggtagcgttt ggggattacc 6060 tcctcctttc ggatatggaa cccattgtca ctaccattgc agcccgcgtg tggccccaga 6120 gtttcggggc atactgacct gccgtggccc tttccttcct ccgcattaac tgcggcggtc 6180 ccgctaattc gccccactgc tccggagagc aatggtggca actagaggca aggatctcgc 6240 tcgttacctg acttaacagg acatctcacg gcacgagctg gcgacggcca tgcaccacct 6300 ctcagcttgt ctggtagagt cttcagcttg accttcacac tgctgtctct ccgggtaaga 6360 tttctggcgt tgactccaat tgaaccgcag gcttcacccc ttgtggtgct cccccgccaa 6420 ttcctttaag tatcatactt gcgtacgtac ttcccaggcg gcaaacttaa cggcttccct 6480 gcggcactgc actggctctt acgccaatgc atcactgagt ttgcattgtt tacagctggg 6540 actacccggg tatctaatcc ggtttgctcc cccagctttc atccctcacc gtcggacgtg 6600 ttctagtaga ccgccttcgc cacagggggt catcgataga tcagaggatt ttaccccttc 6660 ctaccgagta ccgtctacct ctcccactcc ctagccgtgc agtatttccg gcagcctatg 6720 cgttgagcgc atagatttaa ccgaaaactt acacggcagg ctacggatgc tttaggccca 6780 ataatcctcc tgaccacttg aggtgctggt tttaccgcgg cggctgacac cagaacttgc 6840 ccacccctta ttcgccggtg gttttaagac cggtaaaaga tttctttagc agaaaacact 6900 cggattaacc ttgtcgtgct ttcgcacatt gcaaagtttt ctcgcctgct gcgccccata 6960 gggcctgggt ccgtgtctca gtacccatct ccgggcctct cctctcagag cccgtatctg 7020 ttatagcctt ggtgggccat tacctcacca acaagctgat agaccgcagt cccatcctac 7080 ggcgataaat catttgggcc acaaaccatt ccaggcatag tggcctatcg gatattattc 7140 tcagtttccc gaggttatcc ccgtccatag gttagattga ctacgtgtta ctgagccgtc 7200 tgccttgtat tgctacaatg actcgcatgg cttagtatca atccgatagc agtcaggtcc 7260 ggcaggatca accggattca taattggatt attttttttt tgttaagtac gcttgtactt 7320 ttggaattga acagaatgca cataatcttc acatctcaga tatgaccctt cgatcatacc 7380 ctcattctgt gtgcgtaact ggaggccagc gaatcacaat atggtacaat accatgcatt 7440 catcgcaagc gccgctcttg cgtcacgtac gatcggatcg gcccgtccat gggcatataa 7500 accatcgccg atttccgccc ccggcagccc cgatcagggg ccggatctgc ctgtatgatg 7560 gcgatccgcc ctgattaaat tatgggggga gcggcctgct gccgtggatc tggaacgcga 7620 gtacagggca aagaccggcg gctcggcccg gatctttgcc aggtcgaaaa agtaccacgt 7680 cggcggggtc agccacaaca taaggttcta cgagccgtat ccgtttgtga caaggtccgc 7740 gagcggcaag cacctcgtcg acgtggacgg gaacaagtat gtagactact ggatggggca 7800 ctggagcctg atactggggc acgcgccggc gccagtcagg tcggcagtag aggggcagct 7860 tcgccgcggc tggatccacg ggaccgtcaa cgagcagacg atgaatctct cggagataat 7920 acgcggcgcg gtaagcgtgg cagaaaagac aaggtacgtc acgtcgggga cggaggccgt 7980 catgtatgcg gcaaggctgg cgcgcgcgca tacgggcaga aaaataatag caaaggcgga 8040 cggcggctgg cacgggtacg cgtcggggct gctcaagtcg gtcaactggc cgtatgatgt 8100 gcccgagagc ggggggctcg tcgacgaaga gcactctata tccattccgt acaacgatct 8160 tgaaggttcc ctggatgttc ttgggcgcgc aggcgacgac ttggcatgcg tgataatcga 8220 gccgctgctg ggcggcggcg gctgcatacc ggcggatgag gactatctgc gcggcataca 8280 ggagtttgtg cattcaaggg gcgcgctgct tgtcctcgac gagatagtga cagggttccg 8340 gtttaggttt ggctgcgcgt atgctgcagc agggctggac cccgatatag tggcgctcgg 8400 caagatagtc gggggcggat tccccatagg ggtgatatgc ggcaaggacg aggtgatgga 8460 aatctccaac actatatcgc atgcaaagtc cgacagggcg tacatcggcg gcggcacatt 8520 ctctgcaaac cccgccacga tgacagcggg cgcggcagcg ctcggggagc tcaaaaagag 8580 aaagggcaca atatacccga ggataaactc catgggggac gacgcaaggg acaagctctc 8640 aaagatattt gggaacaggg tatccgtgac cggaaggggc tcgctgttca tgactcactt 8700 tgttcaagat ggcgccggca gggtctcaaa tgctgcagat gcggcagcct gcgatgttga 8760 gctgctgcac aggtaccacc tggacatgat cacccgggac ggcatattct ttctgccggg 8820 caagctgggg gccatatcgg cggcgcactc aaaggccgac ctcaagacca tgtattccgc 8880 atcagagcgc tttgcagaag gcctatgagg tatagcgccg gaggaaactt tgattatacg 8940 ggcgtgctgc cccggggccc atgatactct tcggcaagag cgaccccgcc gagctggtgc 9000 gccaggcgga cctcctgtgc agcaagaacc agttcagggc ggcaataggc ctgtacggga 9060 aaatcctcaa ggacgacccg cagaacaggg gcgtcctgca caaaaagggg ctggcccaga 9120 acagggcaaa aaagtactct gatgcgatca cgtgctttga ccggctgctc gagcttgaca 9180 acaaggacgc gcccgcgtac aacaacaagg ccatagccca ggccgagctc ggagacacgg 9240 catccgcgct ggaaaactac ggcagggcca tcgaggccga cccgcggtac gcgccggcgc 9300 gcttcaacag ggccgtgctg ctcgacaggc tgggcgagca tgaggaggcg ctgccggacc 9360 tcgacagggc agccgagctg gaccgacgca agccgaaccc gaggttctac aaggggatag 9420 tgctcggcaa gatgggcagg cacgaagagg cgctggcctg cttcaagggc gtgtgcaaga 9480 ggcatcccgg ccacgccgac tcacagttcc acgtggggat agagcttacc gagcttggca 9540 ggcacgccga ggccctcggg gagcttgcat cactgcccgc ggagcaccgc gagaacgcca 9600 atgtattgta tgccagggcg cgcagcctct cgggccttgg cagggaggac gaatccatag 9660 cgcacctgca aaaggcggcc aaaaaagatt ccaagacgat aaaaaagtgg gcccgcgcag 9720 aaaaggcctt tgacggaata cgggacgatc ccggttcaaa aagatagccg gctagaggat 9780 cttttttctt gccgcgtcaa tccgcatcat gcggaccttt tttttgggcc ccacaagtcg 9840 cgattcatag actggtacat agaccacctc caccgccttt gcggcaaact cctcccgcag 9900 gtcgcgcatg ccgtcaggcg ggggcccgcg cagcttctct tttagttttg agagcgcctc 9960 ttctgtctcc acctcggggc tccgcacatt ctctgacgca tcgagtatcc tccgcgggta 10020 cggctccacc gcgccgggcc ccgtcttgta gggaaagtcc gtctcgccgc cgtgccggtc 10080 aaggcacatc atcccttctg attccgcaaa gacatgctct tctagctcga ggtcgaccct 10140 gttcttgccg agccctgccg agagcgtctt gtgtatgcgc gacttggacc ttatgggaaa 10200 gacgccgtcg cctagcacca cctcgatcac gttctggtcc accttgatcg ggtgaaccgc 10260 ctttctgaaa aaatccgcag agtacctggc ggagacccgg atcagcgcct cgtggaccag 10320 ctttacagaa tgcacatgga cgtcttcttt ccgcggggcc ctcataaggg ccctaaaggc 10380 acccgtcttc tttgcctcta tcatggcccg agccgactcc tcagtcatgg cgttccgcag 10440 gaccgccgtc ctggtctttc cagtcatccc ctgccgcacc ccgcataagg catactatac 10500 aacgcaaggc aaggtaataa tagcctgccg tctgtaacgg ccgtatgagg tcggagggca 10560 ggcccggata catcgaaaag ttcctaaaga gggcggacaa ggcgatagac aatgcagtcg 10620 agcagggcgt caagagggca gacgagatac tagatgacgc agtcgagctc ggcaagatca 10680 ccgtgggcga ggcgcaaaaa agaagcgatg tgctgctcaa gcaggccgag cgggagagca 10740 agcggctcaa gtcaaggggc gccaaaaagc tcgaaaaggg cataggggcg gcaaaaaaga 10800 tggcagccgg caagggcgac gcgctagaga ccctggcaaa gctcggcgag ctgagaaagg 10860 cggggatcat aacggagaag gagtttcgcg ccaagaaaaa gaagcttctc gcggagatct 10920 gacttgaagc cgctagacta tacccgggac ggctcgataa aggaggtcac aaagaggtgg 10980 tttataggca cgccgtccct tgtcgacctt gcaggcgagc tcggcatatc tgagagcaac 11040 atattccacg tgacatttcc cgacggcgca aagaccaccc tgcatacgca cgagggcggg 11100 cagctgctca tagtgacctc gggcaccggc agcatgtcaa tatttgaaaa gaccggcgga 11160 ggcgaggcgg aatttgcaat aaaagagaca gacaggatac cgctaaagca gggcagcatc 11220 cagtacatac ctgccggcgt gctcacgtgc acggcgcaac agacggcacc accctgtccc 11280 atatagcggt aaactacccg tcgccatcgg gaaaggagcc gtatacatta tggtatgaat 11340 ccgactttgc cagccgggtc accggcgtgc tgtaaattat attatttgag cctctccagt 11400 atcgacaggc ttacaaggtt ggtcatcgtt atccccttgc ggatcacttc ccttctagtc 11460 ttttcgcagt acttgttgac gctctggtgg ctcttttcgc tggatacctc gagcaccaca 11520 atgttcccgc tgtacgggaa ggcaaacttt ggcggaatcc tcgagcatac atccatgcgc 11580 cctattcccg ccctgcctat cttctccttt cttgccacta ccgacgccac ctcgcgtatg 11640 tcctcctcgg aatctccgta ttccagatag tacatggata catagctcat cccgggggat 11700 tccctttcga atatctcctc gtccatgctg aataaatagg aagggccgcc cccgcggccc 11760 tccaccgcct ttatcatttt ggggccgttt ttgaaccttg ccagcaggta gtggctggcc 11820 tgctgctcaa agtacggctt gcttttggac gagaaagtgg cggtcacaaa gtacatctcg 11880 cctacgttcc tcgacgagga ccattcctcc ttgagctcta tcgaggcatg gtcgtgcgtg 11940 tatggcgtgc aggcatatcc ccccggggag gcctccgtct tggacatgca tgggatccgc 12000 ggaaccggtt aatatctagt tccatgccgc cttggggggc gggggccccg cctgtggccg 12060 gccccggggc aggcgtgcgt ggatccatgc gatagttatt taaaactagg atgccgatca 12120 cggatcgtcc caagctagct cagcctggta gagcttccgg ctgtagatgt cggccttggc 12180 tgaccgtata acagcatatc aggcatacag agaccgggtt gtcgaaggtt caattccttc 12240 gcttgggacc acataaaact gccgcgggta caccgcgcat gccgctgcgc agtgcatgca 12300 atgtgcccag tttgcccgcg ccgtgaaaga tggaattctg tccgtgcact gccgcatata 12360 tgccgcggcg cgcctgcatg ttgtgccctg ctcgtacgcg caaatgtcag gagctgccgc 12420 gccaaaagac ggcgcgttca ctgccgcgca tatgccgcgg ctgcatgctg tgccctgcct 12480 atacacggaa agatcaggag ctgccgcgcc agaacactgc gcggcgcgtg cgccgcgcgg 12540 cagggccgcg cccgtccgcc gcatcgcgcg accgggacct ctgccgctcc agcaatgtat 12600 cgagcgccga gtcgtcgact agagtgcgcg ccggcaggcc gcctggcgtc ggcacgccct 12660 gcatccccat ggcccggcgc atctcatcgt tctccctccg gagccggctc tccttctcat 12720 caagcctgct gctcatcctg tcgagaaaca tcacatccga gttgtataga tccctgcgct 12780 gctccatcat gcacagtatg tggcgcaatc gggactggtc gcatattccg gatgccatga 12840 gctccatgac cccgtctttt gtgtgcccat tctgattccc cccgggccgc cttgcggccc 12900 cgcgcatccc ggacctcatc gccgcttcct caggtattcc cggactatcc tgttggcaag 12960 ccgggtctcg tctgtcccct cgcgctcggc cagcctggag agctttcttg cgccgttctt 13020 gcccagctct attggtatct ttttcttgat gcccaccttg cgcatcctct ttagtattat 13080 cttgtggccg gagcgggggc tctgggcaag caacctcagg tagatccgcc tcgacggcct 13140 gtcgagcttt gctatctttg ataccacctt gagcgcctgg gatatggtcg gcaccgcctg 13200 gtagagcctt gtcgcctcgt cccgggatat ggtgcccggt accatcgcct tgatcttgtc 13260 cggtacgccc gcaaagccgt ggtatttctt gaacgtgggc atcgacatgc cgagcttttt 13320 tgcggcctcg gattttgtcg tctgctcggc caggaacttg catgcgtctg caagctcccg 13380 cgggctcatc tggagacggt gcaggttctc tacaaccgat gcggcctttg catcatccag 13440 gccgtactct gtatccttgg ttatcaccag aaacttggac ttttttgcgc ccaggtactt 13500 gagggccgca agccggtggt gccccgatat gaggaggtac agccccctgc cgcccctctg 13560 tatgacgggc gggttctgca gcccctctga tctgatcgac tttgcgatat cccgcacccg 13620 ggacctgtcc agcctccttg cctgcgcctc cttccacaca tgcacatttt tgaggggcac 13680 ctcgcggagg gtctgcttta tcttgggctt gtagcgccga accaacgtac ttttcaagat 13740 gcggatcctt gttaactgtg tttggtaagt ttatcacaac aattaggtta gatagagctg 13800 ttcccacgcg gcaatcccct gtatacgcac gcaaatccgc gcatactccc ccgggaggcg 13860 ttctggggcc ccggggctca cgagcccgga acctggggtg ccccgcgggg gcgtcgatag 13920 aataaatacg cgcagggggc cccgtggcgc gatcgcccgt gctgataata aactgcaaaa 13980 actacaagga ggcggccggc ggcagaattg acagcctagc ggcggcagcc gccggggcgg 14040 ccgcaaaata cggcgtcagg atagctcttg ccccgccgca gcacctgctg ggcgcagtaa 14100 agggggaaga tcttacagtt ctggcgcagc atatagacga caagggggtt ggaagcacca 14160 caggatatgt cgtgccggag ctgctgggag aatccggcgt ctctggcgcg ctcatcaacc 14220 acagcgagca ccgcgtatca gctgaccagg tggcaagcct tgtgcccagg ctcaggggtc 14280 tggatatgat ctccgtggtc tgtgtaaagg attccgccga ggcggcaaat ctctcccggc 14340 accggcccga ctacatagct atcgagcctc ccgagctgat aggctcgggc aggtccgtct 14400 catcggagag gcccgagctg ataggggagg cagcagaggc catcaggggg gcggatggaa 14460 caaagctgct ctgcggggcg ggcataacat caggcgctga tgtgcgcaag gccctcgagc 14520 tcggctccaa ggggatcctc gtggcaagcg gggtggtaaa atcatcagac cccgctgcgg 14580 ccatagccga gctggcacag gccatgtcct gagtactagg cccccgcgtt attgaggcgc 14640 gtcagcaggt caaacgacga cctgcgcagc tcatccggcg acttggcgcc cgctatcacc 14700 atctttcccg acgcaaagac tagaaagctg cagctgtcca gccccttgag tatcatcccg 14760 ggaaacgacc cgggatcata tacagcgcca ggcatgcgcg acgatatcct gtctatggga 14820 acattcctac cggcatccac cgtggctaca atattgcgca cgacgggcct tatcttgcag 14880 tcgccggcag ccccgttgcg caccaggtgg agccgcgcct cgtgcagctg cccaaacgag 14940 gccctcacgg atctggcgcc gacggatatc atcttgccag aaatgaatac agtcaccctc 15000 ccctgcatgc cgggcgtctt tatgtagccg cacctgccgc cgtatacggc ctcatcatac 15060 atgcagcacg gcatggcggc catctttttt gcgctcaccc tttgtacaag gtctgatgtg 15120 ctgacgacat tgacgacccg gggccgcgtc cggggatcca gcattggtca ggatccgccc 15180 gtgcgctatt ttaaccgggg cccgggcggc cgcctcgcca tcttgtcata cttgcgcttc 15240 atcaaaatta cagtgaccat cagggttatg ccggccacgt tggtccctat tatgtagacg 15300 tcccatatgt gcaccccgta tgttatccag agcacagagc cagcccctat gaacatggtt 15360 agataccacg agacgtccct gaggctcttt gtcttgtacg ccttgattat ctggtgcacc 15420 catccggaga gtatcagtac gccgccggcc acggccacga catccagcag tgctatatcc 15480 acggtggtca tttgaaaaag aactgctcca ttccagtctg ctttggcttg cccagcatct 15540 cgtcaaagtc aaggcccatg gacgaggtga gctggtccag agtagactcc atgaactcta 15600 gatactttga cgtgtccacc tctcctgcct gggccatctc gacaggcttg acgcctgtct 15660 tgttcatcac ctttacgtac gatattatgt cgcctttttt gacctccctt gcgttctcga 15720 gcagtctggc cgcccgtatg tgctgcggga cggtctttac gtattcagag ggcgccttgc 15780 ttatcatcac attgaacgcc agatccgcca gcgggacccc cctctcctcc agcctcttcc 15840 cggatgccgc tatggccttt gagatcttta gctttgccga ttcaaactcg tcctcggtct 15900 gtacagccga cagtatgtcg agcagcgaat agaacagctc ctttatgaac gggggcgtgt 15960 gcgacttttt ccccgtcagg cccttgacgt cgaccttgcc ggactttgtc accccgaaat 16020 agtttttctt cctgttagat agcacgacat acctgtactc tttgtccacc tcgagctcga 16080 cgccgtgctc ctttttggcg tgctcgacta tatcatggat ctgccgctcc tctggattct 16140 ttatgaacag cgaatcggtg tccccgtaca gcacctttac gcccatctgc tcgcagtgcg 16200 atatggtctg catgatgata tagcgcccga ccgccgtggt ggcctcggcg gcaggcagaa 16260 agtacagcgg gaatatctcg gcgcccatca ccccgtagct tgcgtttagc accaccttga 16320 gggcctggct gatcacagta tactgctgcc gctgctcctc cgttatagac tggctctttg 16380 agaggctctt gtaatagttg acgcgcaggt cgcggagcga tcctattatc atcgatgtaa 16440 gcccgttgtt tttcgtgcat acccagtggt tggtatcggg gatggtgttc tttctgcatt 16500 cgggatgaac gcacctgacg gtctcgtacg agaggtttcg cacctttatt atgctaggat 16560 acaggcttgc aaaatccata actgtaacat caaagtgtat gccctcttca ggctcgacta 16620 cgagaccacc gcggaacttt ttgtccttga ttacggcgtc gttgcttacc tgttgagacc 16680 tcttttccag ctcgtccctg cggggtatca gcgcgttgcg ctgcctgtgc tcatagtaca 16740 gcaggctcct tatccactgc gagacgccca tgcgggacat atcatcgatg ggcatccggg 16800 caatcctgct ggtcaccacc aggaggtcca tcagtatctc gttcccaaag gtgctaagct 16860 ccagcgtcag gcgcgcgtca tgatagcaat agtttgcagt ctggtataga gtgagatccc 16920 cgagagacac gccataatcg accttgccct cgccgagcat cgccttggac acgctgttca 16980 gggagtaatc tgtatacttt gccgcaaatg catacagctg gaacgacctg ttcgagaagg 17040 tcctgtacag gtccagatgg acgccgtgcc ggagcgtggc cgaatcccgc atcatgtaca 17100 ggggtatgtc ggaatccgcc acgccgaggc gccgggcccg attgtacatg tacggcatgt 17160 caaagtcgtc cccgttgtat gtaagcacaa acgggtacga gcctattatt gctagcgcgt 17220 cgcggatcat gtccgcctcc ttgtcctcgt cgtagaacac cacctcgacc ccgggggtca 17280 catcgtttgc gccctcgtcc gcgccgctct tcaggacaag gacctttctg aggccgtcgg 17340 tggcggcaaa ccccactgct gtgaccctcc tgtccgagat cttggcatcg gggatcctgc 17400 cctcctctga atccacctcg atgtcaaagc tgaggcgcct tatccggggt atgggctggt 17460 tgagcaggtc cgcccacccc gctatgaact cgcggaactc tttcctgtcg gccatgccct 17520 cgtctatgag cttgtcccag agaaggctct tgagggccag ttttacctcg tcggatattg 17580 gcatgtcatg cggaatcacc tccccgcctg ataccgaata gtacctgccc actaccaggc 17640 ccgcgtcata cagatagttc tcgtaatact ttatgtcgga ttcccacgtg tctatcacgt 17700 ttctgatgct cttctccgag tgggtcccgc ctatcgcaag aggatcagag acggttatct 17760 tggagacggg cacctccttg tcggctatca ggtcgtgccg catgacctgc tctatcccga 17820 gcacgtcctc cctgccccca agaaagccga gctcggaggg cggcagcctc gtataacagt 17880 agggcttgtg ccccgtgttg tccgtccagt ggatgatctt ttgcgattcc gactcgtaga 17940 acttgaggac cacggccctt gcctggccat cataggttgc agatacaagc agtgacgggg 18000 gaatctcttc atcctgtgca gtcagcgcac cggcacctcc tttgttcccc cgggcatcct 18060 tgacgcccag tatgtgatct tctccttgtc catcatggtt atgctggccg atgcatcttt 18120 taccagcttg gaggcgttct ccggatatgt atccaggcag acaaaccgcc tgattcctat 18180 ggtcaccgcc atctttgtgc actctaaaca cggagagaac gtcgtataca tggtggcgtt 18240 gcctccccct gcgcctattc ccagtatcgc acagtgcatt atcgcgttgg cctctgcatg 18300 gttgcacagg caccggtcca ggccctcgcc tgagcggatc ttgccctcca tgcgctctat 18360 gcacctttcg cacccgccct cgaagcagtt ctttacgccg gggggcgtcc cgttgtatcc 18420 tgtggccagc tgcctgtgat ccctgactat gacggccccc acctttctga ccatgcagtt 18480 ggatcggagc tttgccagct ccgcctgcag catgaaatat tcgtcccagg acgggcgctc 18540 aaaaccgctc aacggccatg gtgccaccgc ccggcatatt atggtatatg ccccggtgta 18600 cgaaaccata aaacaacagg ccgcgtcagg gccgcgcgtg gagaccgcac acataacggg 18660 caaatacgta gagcccggcg ccgtcgagag gcgcgactac caggtgggcc ttgccgagca 18720 ggccatacgg gaaaactgca tagtggtgct gcctaccggc ctcggcaaga cggccgtggc 18780 cctgcaggtg atctcccact atttggacga aggcaggggg gctctcttcc ttgcgccgac 18840 aagggtgctg gtaaaccagc accgccagtt cctgggcagg gcccttacca tatccgatat 18900 taccctggtc acaggcgagg acaccgtccc gaggcgcaaa aaagcttggg gcggcagcgt 18960 gatctgcgcc acccccgaga taacaagaaa cgacatagcg cgcggaatgg tcccgctcga 19020 acagttcggc ctggttgtgt tcgacgaggc ccacagggcg gtgggcgact atgcctattc 19080 cgcaatagcg cgtgcagtgg gggagaactc tagaatgatc ggcatgactg cgacccttcc 19140 aagcgagagg gagaaagccg acgagataat gggcactctt ctctcaaaga gcatagcaca 19200 aaggaccgaa gacgacccgg atgtaaagcc ctacgtgcag gagaccgaaa ctgaatggat 19260 aaaggtggag ctgcccccgg agatgaagga gatccaaaag ctcctgaaga tggccctcga 19320 cgaaagatat gcggccctca agaggtgcgg ctatgatctc ggctcgaaca ggtcgctctc 19380 ggctctgctc cgccttcgca tggtcgttct aagcggcaac aggcgggcgg caaagccttt 19440 gtttactgcg atacgcatca catacgcgct caacatattc gaggcccacg gggtcacgcc 19500 gtttctaaag ttctgcgaga ggaccgtcaa gaaaaagggc gccggtgttg cagagctgtt 19560 cgaggaggac agaaacttta caggggccat ggcgcgcgca aaggcggcgc aggcagccgg 19620 catggagcat ccaaagatac caaagttgga agaggctgtg cgcggggcca aagggaaggc 19680 gctggtcttt acaagctaca gggactctgt cgatttaata cactcaaagc tgcaggctgc 19740 cgggataaac tcggggatcc tcataggaaa ggcgggagaa aagggcctca agcagaaaaa 19800 acaggtagag actgtcgcca agttccgcga cgggggatac gacgtgctcg tatctacaag 19860 agtgggcgag gagggcctcg acatatcgga ggtaaacctt gtggtattct atgacaatgt 19920 cccaagctcg ataaggtatg tgcagagaag gggcaggacc ggcaggaagg acgcgggcaa 19980 gctggtggta ctgatggcaa aggggactat agacgaggca tactactgga taggccggcg 20040 caagattact gccgccaggg gcatggggga caggatgaac aagtcgcttg cagcgggggg 20100 ccctgcgcca aaggcagccc caaaaaaggg gctcgagggc tatttctagg cgggcttatc 20160 ccaggcgctt tatcacgtgg tagccaaact cggattttac cggctcggat acctcgccta 20220 cctgcaggcg gaacgcggca tcctcaaacg gctttaccat cttgcccctg ccaaagtagc 20280 ccaagctgcc gtccctcttt gcgctgcccc cgtctatcga gagctccttt gccagctttc 20340 caaacttttc gcccgccttg aggcgctctt gcactgcgag cgcctcgccc tgctttttta 20400 ccagtatgtg cgagcacttt atcttgtccg ccatgcgcgc gcacattcca taccctgcta 20460 taacttctcg gtatgcaggg ccctgccggc cggcagatct tccgggcggg cccgccaagc 20520 tagactttta attgggatcc ggcggggcgg cgcatgtctt tgtattttac gataaagacg 20580 gccaacctgg ccctgcccga cgtggtaaag aggtacaacc acgtcctggc gtgcaagagc 20640 gaggtgatga gggccgagaa gcagatccag gtgtccatct cgtcgtcggg cggtctggac 20700 aagtacgcgg agctcaagca gcagttcaac tcgaggataa ccgagttcta ccgctcgata 20760 gaggagctgg agaagacggg cgtggtggtc aagagcatag acgaggggct cctggacttt 20820 cccgcaaagc gctttgggga cgacatctgg ctgtgctgga aggtgggcga gcgcgagatc 20880 aagttctggc atgaaaagga ctcggggttt gacggaagaa agcccataga ggtaagtgac 20940 gagtcactag tgtagatgct ctcctcctgg ctgcgcgtaa tacgcgtccg gttcctgctc 21000 gcgtcggtga tagccgtatc agcgggcctt gccctctcct ggtggcacgg ccacggaata 21060 gacgcgctca cagcggcact caccatggcc ggagtggccg ctcttcatgc aagcgtggac 21120 atgctcaacg actactggga ctacaagcgc ggcatagata cgagaaccaa gaggaccccg 21180 atgagcgggg ggacaggggt gctgccagag ggcctgctga gcccccgcca ggtgtaccgc 21240 gccggcatca tatcactggt gctcgggact gccgccggcg catactttgt gatcacaacg 21300 gggcccgtca tagctgcgat actcggcttt gcggtggtct cgatttactt ttactcgaca 21360 aggattgtgg actcgggcct ctccgaggtg ctcgtcgggg tcaagggggc gatgatcgtc 21420 cttggcgcct actacataca ggcgcccgag atcacgccgg ccgccctcct cgtcggcgcg 21480 gcagtggggg cgctgtcatc tgcggtcctc tttgtggcgt cgtttccgga ccacgacgca 21540 gacaaggagc gcggcagaaa aacgctggtg ataatactgg gcaaaaagag ggcctcgcgc 21600 atactctggg tctttccagc tgtggcgtat tcatccgtga tagcgggggt gattatccag 21660 gtgctgccag tgtactccct cgccatgctg cttgccgccc cccttgcggc aatatcggca 21720 aggggccttg ccaaagagta tgacggggac aggatcatac gggtcatgcg cggcacgctg 21780 cggttcagca ggactgcagg cgcgctgctg gtgctgggaa tactgcttgg ttgagtggaa 21840 ctagactcga gactgtgtaa gcataagatg ggcatgcgat caagtaccag aaccgataga 21900 attattctcc ataaaatcat ggaattccca caccccctga taaagatctg aagatctctg 21960 cccctctgac ggaccagtcc agacgaaagc gccatctcat caaaagggtc ggtatttgaa 22020 ttagtcacgt atgttgggga cgaacgtagt gaagtaccag cacaatctgt ccatcttcac 22080 ccagatcatg cgattctaac tgcaccatga gagtcaatcg ggtgtaaata aattgggatc 22140 acttattcta ctatcacgtt atcatctgtc atgtcaacga agatggtttg tataatctgc 22200 gggttcctaa tatctccaat gtcatcagaa gttacttctt tcgtttctcc atcccgcaga 22260 gtaacattgt gctgtcccat aggctgatag agcttctatg tatatgaaaa cttaccaact 22320 ttacagggaa ttggaagata aatcaagggt tgttgaataa gtcgactagg aggcagcata 22380 gtataatctc ctttgtacat tatgcgtaca tagccaccaa ccggttgtaa agcgacaccc 22440 tgatcaggat ttcccgcatg atgctccctg ccttcctggc ctgcacggac tcgccgaata 22500 tctttttgga cccctcaaac accatctcca cggatgtcca gcagtcgtat ccaatatcct 22560 tgttccactc gtcgtaccgg cccacctagg ccttgaccac gagctgcccg gcacggcagc 22620 ccgtgcccaa cctcgagttt atcctgatcg tggagatgaa catgtgtaac tgcagtcacg 22680 gaaaccttca gtttctggac agctatcttg ggtctagctt aaacatacgc aacgtattgg 22740 cagaattact agatttctat ggtcatgctt tcttctgtca ctctatcgaa cacggcacgt 22800 ataatgtgtg tgcgcttttc atctacaaga cttgagcagg ttattacttt tgattccccg 22860 caccgcatag taacgtcttg tatgtccatt atgaattaag acatctacgc gtataaaaac 22920 atagtattgt taccggggcg gggctacccc agggtagcat catccccctt gtacatcatg 22980 tgagcatggc cacaaaccgg ctgcaggcca acacctcgat caagatctac cgaatgatgt 23040 cccctccctt cctggcctgc acggcatcgc cgaatatctt tttgaacccc gcaaacacca 23100 tctccacgga tgtcctgcgg ccgtatccaa tatccttgtt ccactcgtcg taccggccca 23160 cctaggcctt gaccacgagc tgcccggcac ggcagcccgt gcccaacctc gagtttatcc 23220 tgatcgtgga gatgaacatg tgtaactgca gtcacggaaa ccttcagttt ctggacagct 23280 atcttgggtc tagcttaaac atacgcaacg tattggcaga attactagat ttctatggtc 23340 atgctttctt ctgtcactct atcgaacacg gcacgtataa tgtgtgtgcg cttttcatct 23400 acaagacttg agcaggttat tacttttgat tccccgcacc gcatagtaac gtcttgtatg 23460 tccattatga attaagacat ctacgcgtat aaaaacatag tattgttacc ggggcggggc 23520 taccccaggg tagcatcatc ccccttgtac atcatgtgag catggccaca aaccggctgc 23580 aggccaacac ctcgatcaag atctaccgaa tgatgtcccc tcccttcctg gcctgcacgg 23640 catcgccgaa tatctttttg aaccccgcaa acaccatctc cacggatgtc ctgcggccgt 23700 atccaatatc cttgtcccac tcgtcgtact ggcccacttg ggccttgacc acgaactgcc 23760 cggcacggca gcccgtgccc aacctcgagt ttatcctgat cgtggagatg aacatgtgga 23820 actgcaggca cggaaacctt cagtttctgg acagctatct tgggtctagc ttaaacatac 23880 gcaacgtatt ggcagaatta ctagatttct atggtcatgc tttcttctgt cactctatcg 23940 aacataaaac gtataatatg tgaacgcttt tcatctacaa gacttgtgga ggttattact 24000 tttgattccc cgcaccgtag agtaacgtct tgcatgtcca taagggatta agacatctac 24060 gcgtataaaa acatagtatt gttaccgggg cggggttacc ccagggtagc atcatccccc 24120 ttgtacatca tgtgagcatg gccacaaacc ggctgcaggc caacacctcg atcaagatct 24180 cccgaatgat gtcccctccc ttcctggcct gcacggcctc gccgaatatc tttttgaacc 24240 ccgcaaacac cgtccggttc cccgtaccag ttcggcatgc agacgtcttt aatcaggccg 24300 acaactctgc cgtagttgta ccctccaggt ttctccatta caaggccgga atcttttgat 24360 ccaaatattt ttcggcgcac gatggcgcct aattaggcag ggttagtcag caggctcaat 24420 caatatgcct attttactct tttacccacg gcttctacca cttttttaca cccattacaa 24480 atgaacctct gtggcccggt gtccatttta atggatgaat ccgtgctgtc ctcatgtgaa 24540 cacaacggac acttgaaagt gtacctgtat ggaaatgtcg ggggggcgtc cttttcaccc 24600 aagacttttt ctatgtatat tgtgcaacat ttggcatgat acaccattcc ccctttcttg 24660 gtataatgtt gagtgcccgc aacatccttc ttgcacagag cacataatcc gggattcgat 24720 tcgtgccact ccacacagga agtatggcat ttaaaatcag aataaggcat gatggagcct 24780 tctagattct tcatgcatat ccctcaaagc tttttcatgt ccatgttctc ccccaaatgc 24840 catattactt aactattctg ttgtgcactg ctgacaaggc cctgaagttt ccgagtactc 24900 aaacacctgt acttcacaac acagtgtaaa acctcggggg atcacaagga aaggagctcg 24960 gtccaaaacc aagaccgata tggatagaga aagagaatac acgccgcggc ttttggggat 25020 ttatccgcgg cgggttgggt gatatcaagt gccagctcag ggggcacgac atgccgcagg 25080 atgtgatgga gatcaaggga cggcaatata catggcactg catgccgcag gtgtggccat 25140 aacctgataa ttgaggcccg cggctgtgcc tacagcacgg gatattggcg cagaacgggg 25200 caatctcaca ctttgtaccc ttcatacaca taaatcccgc ttggatgtgc ggctgcgcat 25260 gatcagcggg catgccacgg ccgagggtac acagaggata gccgagatgt ccggcgcaca 25320 ccatgacaac tacaaggtgg tagacgggct gcacctctcc aacgtgggga tgggcaccta 25380 ccttggcgac gcggatgacg ccaccgacag ggccgtcaca gacgcggtca agaggtcaat 25440 caagtcgggg ataaacgtca tagataccgc gataaactac cgcctccaga gggccgagcg 25500 ttccgtgggc agggccgtta cagagctctc agaggagggg ctggtatcca gggaccagat 25560 attcatatcc acaaaggcgg gatacgtgac caacgattca gaggtctccc tcgacttttg 25620 ggagtatgta aaaaaggaat acgtcggtgg cggcgtcata cagtccgggg acatatcctc 25680 gggataccac tgcatgaagc ccgcgtatct agaggaccag ctaaagagaa gccttgcaaa 25740 catgaacgtc gactgcatag atcttgtcta cgtgcacaac ccggtggagg ggcagatcaa 25800 ggaccgcccc gtgccggaga tcctcgaggg gataggcgag gcctttgcca tgtacgagaa 25860 aatgcgggag gctggccgca taaggtatta cgggctcgcc acgtgggagt gcttccgggt 25920 cgcagagggc gacccgcaga gcatgcagct cgaagcagtg gtaaaaaagg ccaaggatgc 25980 cggcggggag aaccacggct ttaggttcat acagctgcca ttcaaccagt actttgacca 26040 ggcctacatg gtaaagaacc aggggacggg cggcggcaag tcatccatac tggaggcggc 26100 agccgcgctg gacattggcg tgttcacaag cgtcccgttc atgcagggca agctgctcga 26160 gcctggcctg ctgccggagt ttggcgggct ctcgcccgcc ctgcggtccc tgcagttcat 26220 caggtctaca ccgggagtgc ttgcccccct gccggggcac aagtccagcc tgcatacaga 26280 cgagaaccta aagatcatgg gcgtgccccc cattcctcct gacaagttcg gggagcttgt 26340 ggccagcctt acctcatggt cgcccggcca gaaatagtca gcgtgttccc tcgggcatta 26400 tctggtctag cacctttttt ggcagcttcg agtccgcaga atctttcaca ttgcgccggg 26460 tcctgtccac gttggcagga tacaggtcta ttcccgtaaa gcccctcttg aggcacgccg 26520 agactatccc cgttgtgccc cttcccgcaa acggatccag cacatagtcg ccctctcttg 26580 tggcaaactt tactatccgg gatacgaggt cttctgggaa caccgcaaag tgctcgttgc 26640 cgtggtgcgc ctttgtggat atctcccaga cgttcccagg gttcttgccc cgggggttgc 26700 atgctgcaaa tatcggatag tgctcgtggc cccctatccg cttgcgcgtc gcatgccttt 26760 tgaacttgcg gtagcatgtc gggcagtgct tttcggggtc atacccgtgg gcccgcgata 26820 tctcctcggt ggttggcagc tcgtcaaacg gcgtctcggg ggacgagccg tgtatcactg 26880 ctgcaatcct ccctatggct tcagggtccc tcctcccggg ggagaactgc agccggtcgc 26940 gctccggctt cctgttgacc ccgctcaggg cctcgttgcc ctggacgcgt atcgggtcta 27000 tgtcaaaggc gggggattcc gactttgata gcaccagcac aaactcgtac gcctgcgtaa 27060 ggttctgctt cgagctctgt gatagcgcgt ttttcttgta ccagactata tcctcttgaa 27120 agtggtaccc aaggtctaca agtctgagcg cgagccggtg cgggaccatc agcttccggc 27180 gccgcctccg ggtgtcgcct atcactatga agaggctgcc gtcgtcggta agcaggtcca 27240 tgcagctctt gaacacccct gccagctcct cgacgaactc atcaggcgtc ccctcctggc 27300 ccagctcgga gggatccgac ccgtactttc tgtgcccgta atacgggggg gaagtgacgg 27360 ccagcctgta cctgccgcgc tcgccattct tttttgcaag cctgggcagc accgcccggg 27420 catccccccg gataacctgg aaccggttgt tcatgttgcc tgccccacat caggctggac 27480 ggacagatct gcgcagcagg cgggccgcgc gatcctacgg gtcatacaca ttctatgcgg 27540 ctgccccggc gccgacttaa aatcgttgta ggatgcggcg ccgcagatgc attgcccgcc 27600 ttatacaccg cccgggatcg gccgccttgc agcacacgca gtataaacgg gggcccgggc 27660 ggcgcgtatc acatgtggat aaaggacgaa ttcctcggcc cgggcaacaa gatgaggctg 27720 ctctacctga tactgcccat ctatgggtat atctttctgg agtactatcc gttctttccc 27780 tggatggcca cctactggtg gtcagtagct ctcagccccc cgatagtgcc cacgcattat 27840 gccggggagg ccctggggcg gctgatcggg gatcacgtat tgtttggcat caccacaaag 27900 tacgtctatg cggcaatatg gctcggcatg gcccatggga taatcctgct ggcagggcgc 27960 ctccggggac ctaggcaggc gccacggacg ggcatcccat aggctctggg gcatccgcgg 28020 gtccccgcgg tccaattaaa tacagcaagg aacgggtagt ttcgttgaag ctgcaaggca 28080 agactgccgt gatcaccggc agtggtaccg ggatcgggct ggcggtggca aggaaatttg 28140 ccgagaacgg ggccagcgtg gtaatactcg gaaggagaaa ggagcccctc gatgaggcag 28200 cagcagagct caaaaagata gcggaatctg caggctgcgg ggcctcgatc aggatattcg 28260 ccggggtgga cgtggccgac gaatccgcga taacgaaaat gttcgacgag ctgtccagct 28320 caggtgtaac cgtggacata ctggtgaaca atgccggcgt gtcggggccc gtcacgtgct 28380 ttgccaacaa tgatctagaa gagttccgcg gggcagtcga catacacctg accggctcct 28440 tctggacatc gagggaggcc ctcaaggtca tgaaaaaggg ctccaagatt gtcaccatga 28500 ctacgttttt tgcagaagag aggccactcg agcagaggcc gtacaggttc cgcgacccgt 28560 atacaaccgc acagggcgca aagaacaggc tcgccgaggc gatgtcgtgg gatcttttag 28620 accgcgggat aacatcgata gcgaccaacc ccggccccgt ccattctgac aggatataca 28680 agacggtata cccgagggcg gcactcgagt ttgtcagggt ttcagggttt gaggacctgc 28740 agccagaaga agtcgaggtg gcaggcggca ggctaatcca cctgctcggc gcggacgacg 28800 atgcaagaaa aaaaggcata gcagaggccg cagagcactt tgccaagcta aagcccgtgg 28860 atcccgcaaa gctagaggcc acccttgatg ccctgctcgc aaagatcaag gggatagccg 28920 aaaagataca ggccaacact gcaaggatga taccagacgg ggagtttctc tcccaggacc 28980 aggtggccga gacggtactc gccctctgcg atgacaagat ggccaagacg gtaaacggcc 29040 gcgtaatccc cgccgacagg gtattctacc cggtaagggc gcatgtggcc aatgccgctc 29100 cgcgcgtgcc cccgcacgac tattccgggg gatgcgtcct attcatgata gatgcagcag 29160 acgacaggga tgtagaaagg gcgaccgccc tggcatccca tgtggaaagc cacgggggca 29220 cggcagtctg catagtctca gaagactcgc cccgcgcggc aaaggagatg atagcgtcaa 29280 agttccactc gcatgcgagc cacatagaca aggtagacga gataaacagg tggctgagcg 29340 ctgcatcaac aaagataggc cccatatctg cagtggtcca cctgtccggc aggatgccaa 29400 aatccggcag cctaatggat ctctccagaa aagaatggga cgcgctggtt gacaggttca 29460 tagggacgcc ggctgccgtc ctgcacaggt cgcttgagca ctttgcaccc ggcgggcgca 29520 aggacccccg tttgttcaag ggcaagagcg gcgtcatcgt gataataggc cccgacctgc 29580 ccgcggggaa aaaggcctcc ggcgccgaga gggcaagggc ggagatcttc cggggtgcgc 29640 tcaggccgct gacgactaca gtcaaccagg agctcagcga tgtgctaaag tcaaacgtgc 29700 gcctgtttac catccttccc ggcagggcgg acgggggcga gaccgatgat tcccgcatat 29760 ctgctgcaat cgactacttt ctgacccccg aggctgtctc gtccggcgag gtcatattct 29820 gcgtagacga gaacaggggc tagcccgcca cagcgtccca gaagagtttt gctgccacta 29880 caagtatgac cactgccgcc agtatgcgca ggcccctctc cctcaggttc agcgagagct 29940 tggcgcccag caggcccccc gcaaacgcgc cagacgagag cagcagtgca tggtaaaagt 30000 cagtgtgccc cagcagcgta tgggtgacca tgccggtaaa tgccacaaac atcaggacca 30060 gctgcgcggt gggcgcggcc ctccacatgc tcatgcccat tatcgccacc atgagcggga 30120 caaatacaag gcccccgcct atcccaaaga agctcgatat tatccccgcg aaaaagctgg 30180 ccgctatgga tagcagcagg acggtcaggt gggagcgccg ctgcccctcc ccaatcctgc 30240 tgctgagcag caggtatgca gcagatccca ccaggacgat cccaaagaac aatctgaaga 30300 tatcaggcgt tgctgcggcg gagaatagcg cccctagaac ggtaccgggc agcgccagca 30360 ggcccagcgt cagccccgtc cggtagtcta tcctcttctg cctggcatat gacgcggtgg 30420 ccgccgacgc gctgctaaat gccgcaaaga ggctgctgct ggctgcagcc gtcggtgaaa 30480 agcccataaa ggtcagcacg ggaaccacca cgaacccgcc gccaagcccc accatcgagc 30540 cgattacccc ggctgccagg ccaagcagcg gcagccatac ctcctccatg acaacctgcg 30600 gcactgctgt tatgtataat accgtttttg ggggcgagga aacggatatc atgcctagaa 30660 gccgtctgtg ctatccctgc cgacccccaa gccccaccca cttcacactc atctagtaag 30720 caaatcttgc accaagccgg atattttcca tggttttggc aataaattca ataaacagta 30780 cgttcggtca tactgcatgg caaaagagac catagggaac cgcccagtcg atatcatgaa 30840 ggagggcaac gaggtcaaga tagtattcca ccccattcta aaaggggcaa aacaccccga 30900 cgcggccgta ttctcgataa aactgtcgaa aaaagattta gagatcatca ggaatgcttt 30960 ctaacatact gtaaaatctc aaaaacatac tgcaacgtgc gatttatata cgggaaatgc 31020 gatcgcgata tagtgacaaa atataaccga tccgctagtg aaagcatggg atcggagacc 31080 tatgggtttg atgccatacg aggcatgcaa gctaaccacg aatattacgt taccatatgt 31140 cctctaaaga ttattcccaa gctcttcata ttcaacgagt atgagctccc ggcaaagctc 31200 agagctcaaa ggacactccg aaaatctaga attccaaccc tcaaggacta catactaagc 31260 aatcctgacg agtacatatt ctcgtccctc gctgcatcgg tggatgggcg catgaagttt 31320 atcccagccc cgcatctggg gccagatggt aaaatgggca gactccacat agacatgtcc 31380 tccaagctaa taatcaacga cggacaacac cgccgcaagg caatagaggc agccttgctc 31440 gagaagccgg atctgggcaa tgagtcaatt tcagttgtat tttttgagga tcgagggctt 31500 aaacgctgtc agcaaatgtt ttccgatctg aacaaaaatg ctgtcaaacc atccaagtca 31560 ctcaatatac tgtatgacaa caggaatcca ttttctcgtt ttatagtgga catggtagat 31620 gagatagatg ttttccggga cagggtagag ctggaaaaga ccaccatagg gaaaaatgcc 31680 aaggaagcat ttactcttgg gggattgtct gatgcaacaa tgagactgtt cggcaaaaaa 31740 tccttgtcgc gacccagcaa ggaacaaaag ggactcataa aggagttctg gaaatgcgtc 31800 tcagccaaca tgcaagaatg gggacggtta gtagacggcg aaatgtcggc agacgagctg 31860 cgtgcaaact atgtcaatgg ccataccaac tgccttaatt cactagggga ggtaggccga 31920 acagtaatca agcagcatcc agaatcgtgg aaaagaaagc tttcctctct gtctcggatt 31980 gactggtcca gggaaaacga ggtgtgggag ggcaatctta tacagggtaa gaagatggtg 32040 aggaccacca tcggaataat gctcggggct ggcgtcatac ttcgggaatg cagcatacga 32100 gttcctgaag agattgagag gtatgagaaa tgacatctgt gtttgacaag cggacgactg 32160 acagcatata cgacgaggta cgctcagtat acctcaacga tgcacgccct tggatccttg 32220 ggtttagtgg cggaaaagac tcgacatgca tggtacagat tgtatggaaa gccctctcgg 32280 aactacctgc agacaagctg gacaaaaaga tctacatagt gtcgtcggac accttggtag 32340 agtccccaca gatagtggag cggctgacca agtcacttga cagcatagag aaggcggcaa 32400 aagaggccca tattccaata tcgaccaacc tactgcggcc tccgattact gacacattct 32460 gggtccggat actgggcatg gggtaccctg cccccacctc catgttcaga tggtgcactg 32520 atatgctcaa aatagcaaac gctgacaggt tcatcaaaga gagagtctcc gagtatgggg 32580 aggtcatagt tttgctgggc acccgcaaga gcgagagcgc cacccggcag caggtaatga 32640 atctgctgga gatagagaat agcgttctaa gccaccataa aaaattcgca cagacctacg 32700 tgtatacccc cctggtggac tttgaggcgg aggacgtatg gaactacctg ctccagaata 32760 agaatccatg gggcgataac aaccgagacc tacttgccct gtatcaggat gccaatgcgg 32820 cagagtgccc tcttgtggtg gacaccagca cgccatcctg cggaggtggc aggttcggct 32880 gctggacgtg cacggtggta gacaagcaaa agtctctgga cagcatgatt gaaaacggtc 32940 atgaatggat ggaaccgctg gcagaattgc gccacattct aaagcagaca caggatcc 32998 <210> SEQ ID NO 2 <211> LENGTH: 42432 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (3)...(10421) <221> NAME/KEY: CDS <222> LOCATION: (10625)...(11434) <221> NAME/KEY: CDS <222> LOCATION: (11478)...(13046) <221> NAME/KEY: CDS <222> LOCATION: (13046)...(14620) <221> NAME/KEY: CDS <222> LOCATION: (23558)...(24862) <221> NAME/KEY: CDS <222> LOCATION: (24913)...(25728) <221> NAME/KEY: CDS <222> LOCATION: (26504)...(26881) <221> NAME/KEY: CDS <222> LOCATION: (29655)...(30491) <221> NAME/KEY: CDS <222> LOCATION: (34559)...(36067) <221> NAME/KEY: CDS <222> LOCATION: (37002)...(37403) <221> NAME/KEY: CDS <222> LOCATION: (37404)...(38282) <221> NAME/KEY: CDS <222> LOCATION: (39454)...(40572) <400> SEQUENCE: 2 ggatccccgc gccgccagga gagggcagcc ttggcggggt ggcaatatcc gacgacggga 60 ggtacatgta cgcaatcggc agggatctgc tcacagtata ccggtataca atgaacccgc 120 cccatgacat agcctcggcc gcgctcggtg cgcagtcatt ttctctgcct ggcggcatca 180 gccccgcccc cggcgcgccg accggccttg acatctcgga tgacggccgc cacctgtacg 240 tcccggacga aaacggcgtc gtgtacaggt ttgatctgga aagcccgtac aggctagacg 300 gcggcacgtt tggctcttct gtttatgtgg gatccgacgt tgccgcgccc cgcggcgtat 360 acgtggcgcc gggcggcagc ctcatgctgg tctcggatag tgcagacggc accatccaca 420 ggtacgagct ggcaagcccg tacgagccgg cgggcgcggc aaacagggga tcattcgacg 480 tgtcggatat ggacggctcg cctgtcgggg cggggtttgc gggcggcctg cacatgtatg 540 tcgcgggaaa cgacaccgga agggtctacc agtatccggc gggcacgcac cagatacagg 600 aggcagccgc agggccgcgg ctgctctcgg ccgtcctgga caaagacgga accctgaggg 660 cggcctttga cggcacggta gacgcgggat ccgtgcagcc cgggatgatc accatcaggg 720 acggccatgg ctccaacacg ggaatacccc ttttgcttgc cgggggtgcc gcggactctg 780 atgtcatgac atttgtggtc cccgagaaag acagggcaga ggctgccgca tacggggacc 840 agtcgctgca tgttcccgcc gcggcgctgg cggggactgg cggcgggccg tttgtgcccg 900 acttttccgg gggctcgctg ctggcgtccc tgtaccggca cgagcggccg ttccagggcg 960 aggagatggc acggacggag agatccgaca ggtacgcgct tactgtaact gcaggcggga 1020 gtcagatgca tgtgggcggc gccggcggaa acatcacctg gtacgatctt ggcacgcccc 1080 atgacataac gaccggcgtc cgcgcgggat ccgacatcct gccggcgtat ccatccgcgg 1140 gcagaaacgt ggtgccgtca ataacgggca ttgccttctc ggatgacggc atgcggttgt 1200 ttgcagcaaa ccggggcgac cgcattccaa tgtaccagct ggacagcccg tacgacatag 1260 ggagcgccag cctcgaggga accctgttta cggggttcca gtcgggcatt gcattctcgg 1320 atgacggcac gcgcatgttt gccgccctgc tcaccgagaa tgccatacgg cagtacgacc 1380 tggagggccc ctatgacata cgcggggcgg gcaatgcggg ccagtacgac ctggacatcc 1440 cgctgcaccc aggactgctg ttcctgctga cctcgggggt gcacttttcg cccgacggga 1500 cgaggatgtt cgtcggcgag gggatatcag atgcggagga tgccaacgcg aacagggatg 1560 tcaacgtcaa cctgtggcac aggtttgatc tctccacgcc gtttgatgtg ctcacggcgg 1620 agcgcgtgga cacgtacgag tacagcacgg ggccggcagg cgatctcgag gacctctccc 1680 tgtcccctga cggccgcaga ttgtacaccc tgtcgagcga gagggtaagc tcaagcgagt 1740 atacaatcac ccgggcccag tactggctgc cagaaccgta cgacgtgacg ccgccgtacc 1800 atgtgccgtc attcaacgca agccaggggg gcaacctggc agacccctac gggatggcct 1860 tctcgcccga cgggaccagg ctgctggtca cggggcacgg gcagacgaat gcaaagctgt 1920 tccacctgaa tccgcccttt gatgtgggca cggccgtgtt ccacgaccac ggcaggttcc 1980 gccccggggg gcccgcaagc gagatcgagg cgtcggggat atccctgtct gccgacggct 2040 ccaggatgtt tctctccgac cgcggccgcg gggccatcag ccagtacacg ctggttgcgc 2100 cctttgatgt ggagtttgcg tcggatgtgt ccgcggatgg gcagctcgac gttggcgccc 2160 aggatgcgct tcccggcggg cttgccttct cgcccggggg gacgaggcta ttcatggtgg 2220 gaggcatgga caggtcagtt cacatgtatt ccctgaatac gccgtttgac ctgggcgggg 2280 cagagcatgc ggcgtcgttt ggcgtggggg acagggtctc ggatcccctc ggcatcgcct 2340 ttgggaacgg ggggactaaa atgctaatag ccgatacgac aggctttgtg cacgggtacg 2400 accttggcgc cccgtacgat atctcgggcc ccgcgtacag cggcatattt gacgccggcg 2460 gcagcatccg ggacgtggcc gtcggcgggg ggtccatgtt catactcgag ggggagacgg 2520 accgggtgta tgagcaccgc cccggcatat acccggtggt ctcagcactg gacgggccgg 2580 cgctggtctc tgctgcagca gatgcaaggg tgggtgcggc cgaggtgctc tttgatcgcg 2640 cggtggatgt tggcgggata gaccccgggg gggtccgcat agtggatgca gcaggccccc 2700 tgcccggcgt ggtgatctcg gatgccgtca taccaggcga ggatcccggc gtggccaggt 2760 tcagcctgtc ggacgcggag gtccttgccg tgtccgggta tgccgagccg agtctggtct 2820 ttggaaggca tgcggtgccg ggcgcggcag gcggcacatt tccctcccag ataggcaacg 2880 ccacggagct tgtgggatcg attccgaatc cgaccctgga ttttgggacg accctgacgg 2940 gggcggcatt ctcggcggac gggacggtgg tatttctctc agacggcccc accggcaggg 3000 tgtacccgta ttcactgaat atcccctttg acatatcgtc tgcggcgcct gggggctttg 3060 taatcgtgcc cgtcggagtc tcggacattg cgttttctgc cgacgggcgg aacatgctag 3120 tcgcggacga aaccggggga atacacaggt acctggcccg cagcccgtac gagataggca 3180 cggatttcat caaatcatcc ctgggtgagt ttgtcgagac attctcggcg gcgccccgcg 3240 tgcaggatct tgccggcatc gccttttcgc acgacggcat gatcatgctt gcggccggcg 3300 gctcggggtc tgtgcaccgg tactcgctgc catccccgta tgcagtatcg ggggccaaat 3360 acgaggagac ggcgatgatt ggcgggagcc cgtcggggct ggagttctcg tccgacggcc 3420 tgaggatgtt tgttcccgat gcgggctcgg agacggcggc agtctacggc cttgccgccc 3480 cctacgggat tggcgaggcg gagccgctgc cgccgctgtt cctgggggta ggggcagaag 3540 aggccacgct ctcgcctgac ggcaggcaca tcctagttcc cggcaggccc ggcctgtccc 3600 agtactcgct gttctcgacg aatcttgagc tgtgcgcgga gccccggggc attgacgggg 3660 gatcgtgcga agatgggata tacgcctttg agagtccggg caggggcgag ggcgtatcgc 3720 ttgccgcctc gataacggcg gcagacgggc caggaattgg cgagctgcac gggtttgcag 3780 gcccgccgat gccggcgcct gtcatggagc aggtcacact ggattcgcgg gagggcacac 3840 tcagggtcag gctggacagg acagtggacg tcgacacggt gcgcccctat aagatgtggg 3900 tggaggattc agacggcagc cagacaaccc tggcaaattc aacactgttg aatgccgaaa 3960 actcgaacat tctgctcttc aggctggatg atgcggccgc aggcaaaata tccgggtata 4020 catcccccgt gtttcgcacg tggtcgtcgc cgttcctggg cacagacgga gccaccaggc 4080 cccatacgct gggctttgga gacgtgcgcc ttgcggatat atacgatgca tccggggatg 4140 tcccgtcgcc gtcgggcatt gagttttcag atgacggcat gaggatgttc gttacgggga 4200 tcggcacgcc aggcatcaac atattcacac tgtccgcccc ctttgacata acattgccga 4260 agcattccgg ctcaaccaac ataggcggcc tgtccgtgtc tgatctggca tttgcaaaca 4320 atgggaacag cctcacggtg ctcgatgtgg acggggtgtt gcgcgtctac gcccttgggg 4380 acgattacaa tgtggtcacc ggaaccaccc agaagtttag gattacgctc gataccacac 4440 agggcatacc caattccatt tacacatctc cggacggcct gtcacagttt gtggcatatg 4500 atgacaggat tgacttgtac gtgcttggca gcccaaacga catatcgtcg acaaccgaga 4560 taatcccgta ttcgctgcca aggccggacc cgccaaccgg catggacttt acgccagacg 4620 ggcgcaggat gttcctgtcc accgagaacg ggatagacca gtacctgctt tcagaaccgt 4680 ttgcagtcac cacgtcggta tttttgcgca cgatccccat tgacggaggg gcggagggaa 4740 tacggtttgt agacaacgga aggggcctgt ttgtgccggg cgccgacggc atcatccaga 4800 ggcacgagct catctacccg tacggggcca gcacgtcgtt gttggagacc gtcagggacg 4860 gcgtgacgga cggcggtccg ggcgagaacc cggccgccgg agagatccgc cttgcgggca 4920 cattcaatgc atccgataat gtacagtcgc cgtcgggcat tgagttttca ggcgacggca 4980 cggggatgtt tgttaccggg tttggggccg cgggcgtgaa tgaattctcc ctgtccgccc 5040 cctttgatac aaccctcccg gtgcatgtgg aattgcacga tataggcggc cagccggcag 5100 ttgatctggc gtttgcagaa gatggcagga ccctcctgtt gctggccgcg gatggaacac 5160 tggatttcta cagccttgcc ggtgatgcct atgatatagg ggaagcatcc cgtacttttc 5220 aagtgccgtt tgaggatgcc gcgggtgctg tgcccggcgc cttttaccag cctccggatg 5280 gctcgtctat tattgccgca tttgacggca ggattgacca gtatgtggtg atccccttcg 5340 agttcgtgtc atatccactg acaaggcccg gcacgcccac agggattgac tttgcgccag 5400 acgggcgctg gatgttcctg tccaccgaga acgggataga ccagtacctg ctgtcgatcc 5460 cctttgacgt gcgcagcctg acgtatacgg gaaccattcc agtagacggg gtggagggaa 5520 tgcagtttgc ggacaacggc agggcactgt ttttggcgga cagtgaaggc ttgatttaca 5580 attatgacct ggaggacccg tatgctctgg atggcaacac aatttccgtg gaattctcgt 5640 ttgacggtag cgtgatgtat gtgctggagt acgacacaaa aagggtggtc tcgtacgagt 5700 tggagtttcc ctttgacgta tcgagcagaa cacgtgcaga cacgctggac ataccacaaa 5760 ttgactcacc aagacacgtt gcagtctcga tgcccggcaa ccacctgtac ataacaaact 5820 cggtgtttgg ggaagatgac accatacact cctatggaat atctaacaat gacatatcgt 5880 cggcatcata catcggcgag gaaggcatcc cggaacccgt gataaacggg attgactttt 5940 ccaacaacgg ccgccgcatg tttctgattg ggggcaacgg gttcgactac caggtgatac 6000 atgactacat gctaggcaca agatacgaca tatccagcag gagcctgctt gatacatatg 6060 ccattccagg gccggttgtt tttcccgcgg gccttgattt ctcgtttgac aggctgtcca 6120 tgtttataat aagcaccgcc ggttcggtat acaggtacgg cctggacgat ccgttcatag 6180 ttgaaacaat ggactatcag gagtctttcc ggctgcccgt accatcagcg gctgataatt 6240 caatatcgga tctggcattc ggcagcagcg gcctgaatgc cgtaatatcg cacgaggggc 6300 tcgacaccct gtacagcttt gtactggaca tcccgtatgg ggccgaattg gatattgaca 6360 ggcttgagct tccgctggtg ggggttccga cgggattcga gttctcggac aacgggcgcc 6420 agttgtacat tggcgcgttt cgtgactctc aatcctcgcc aggcaccctg cctgcgggcc 6480 tgcagcgcta tgagcttggc ataccatatg acctggcttc ggctgtattt gcgcagtccc 6540 tgggaatatt cgattttcct cccttcaacg gcatgcgggc caatggcagc ttggcaggat 6600 tacatgtgcc gcccgatgga agcatcctgt tcagggccgg aaatgccgaa agaaccgtaa 6660 tcagctatga catggacagc catgatttgg atacattatc attcagggaa tcattcaaac 6720 cagatgtcgg acagtcgaca cccaacataa gggacatgga catatccccg gacggcatgt 6780 tcctctacct gcttcaaggc gatgttctgg acatgtacaa ccttacagat agttattcgc 6840 ttgatgcccc ggcatatgcg ggtaccctgg atttggaacc ggaggatgta atacccaggg 6900 ggatttcatt ctcacgggat ggcacgagtc tgtttatgac aggcgaagac gtggaccaca 6960 ttcacgaata tgcattgaat gaaccatggg acatacgcaa tgccatactt gcaggctccc 7020 tgtccataag cgcagtgaat ggtgcaccgc gggggctgga tatatcggag gatggcacaa 7080 ctgcacatac tatgcgcggg cgtgactttg acacggggcc cgcatccctg gtaaaccaca 7140 tattgccagg ccaatattcc ctgctgacgg atgcgccggc gtttgcatac cccgtggagg 7200 aggagggtgc accgggggat cttgcattct ccgatgacgg catgcgcatg ttcgtggcgg 7260 gcgtaaacaa ccatttaaga cagtacaacc tgctgtcgcc gtatgacact gaaaatgcag 7320 aacatttcat ctcgacggat ctgctgactg cggacagggg ccccacgggt cttgtatttt 7380 cagatgagaa cgactttttc agcacaggcg ccagggccca atttgtgcgc cagtttacga 7440 caaaccgccc gtacgacgca tccacaataa cactgagtga caacggactg tacaaggtga 7500 gcgtggacgg cctgccgtcc ggcatacggt ttacccccga cggcatgaag atgttcatat 7560 cgggccagga gacggccatg atataccagt attccctgcc gtccccgtat gacacatccg 7620 gggcggtcag ggacagggtt gagatagtcg cagggctctt tagaaatgca ggtttgtccg 7680 tcgggttgaa cgagcccagt ccttccggct ttgacttttc ggaggacgga atggagctgt 7740 acgtgacggg gtcgggcctt gttcacaggt atttcctgcc atcgccatac ggcctcgaag 7800 atgcagcgta cgggggcagc ttccacacgt tcagggagag cacgccgctg ggagtggtgg 7860 tgcgggggga tgccatgttt gtggccgggg acagtactga ttccatattg aaatattccc 7920 tgaacgcaca acctgtcggc aacataaccc atgccgatac gcgcgccggg attgccgaca 7980 gggcggagat cgtgtttggg gcaatggcag atacgcgcgc cgagattctc gacggcgccg 8040 atgtagttca taagagtgtg aaaattgacg tattcccaat atcggagggc ataacagtgg 8100 gcagggcact ttatccagag gacgccgcca tacttgatga cggcgcgaat gccacgcata 8160 atagggttgt aatcattgtt cacgacataa cagaaggcga tgcgccgtcc atacatgatg 8220 agccgattgc cgtggggatt tacgccctcg gccctatgga tacaatcgcc gtggttgatc 8280 tccaccgcct ggccgtatcc gcatccttgt ccgggggtga ttccccgtcg gcctcagatg 8340 catccggagt agtggccgag agccgcagaa acgcggtgga caggcctggc gtggaagagc 8400 gcataggaca tggtgtatcc ctggaggcgg ccgacaggcc tgccgtcgac aacatgatgg 8460 atacggatag tgccggcgtg tacgaccgca gtccggacga cgggcccgcc gtatccgaca 8520 ggtccgcgct ggggcttgcc cggatggcag ccgacaggcc tgcagtcgat gacatgatgg 8580 atacggatag tgccggcgtg tacgaccgca gcccggacga cgggcccgcc atatccgaca 8640 ggtccgcgct ggggcttgcc cggatggcag ccgacaggcc tgcagtcgac gacatgatgg 8700 atacgggcag tgccggcgtg tacgaccgca gcccggacga cgggcccgcc atatccgaca 8760 ggtccgcgct ggggcttgcc cggatggcag ccgacaggcc tgcagtcgat gacatgatgg 8820 atacgggcag tgagagcacg agcaggcttg gaccggttga caggccagaa atagtcgagc 8880 gccacagcct ggccgcgtct gtatacctgt ccgggggcga ttccccgtcg gtcgcagacg 8940 gtcatgatgt ggagtccgag ggccgcagag acggggggga caggcctggc atcgacgagc 9000 gtatagtcat caagatctcg tacagccgcg gcgcagccga tgcgcccaga gtggaggatg 9060 caatggagac ttccggcgtg accgcgtaca gccgcggcgc agccgatgcg cccagagtgg 9120 aggatgcaat ggagacttcc ggcgtgaccg tccccaggcg cagtaccatg gacgcgccca 9180 cagtggccga tgaccacagc ctggcccgga ccgcatccat atccgaaggc gattccccga 9240 catttgcaga ggcgcgccgc gcggataccg ttggggatat agacgaggtg gacgcgccca 9300 cagtggccga tgaccacagt ctggcccggg ccgcatccat atccgaaggc gattccccga 9360 catttgcaga ggtgcgccgc gcggataccg ttggggatat agacgaggtg gacgcgcccg 9420 ccgtggccga gaggctcctg gcagtcctcg gcctgcaggc ccctgattcg ccgggagtgt 9480 gggatactgt aggaatagat cactcggaga tttcaggcga tcctgtgccg gagccaagag 9540 tagtgcccag gggcggtggc ggtgggggag gcggttcttc gaaccgcggc cttgaaccgc 9600 atggcggcgg gtatgagatt gactttgagt tccgcataga cggcaggctg gtgctcttca 9660 atgggacaga cgtgctagcc gaatccggca aggacctgct catccgtccg gtgttccggc 9720 cggaggggag tttcaacata tttgatatgg aggtgttgtt taccgccccc ggcggggaga 9780 tatcgactgc ctactacaac agggctggaa tcctcatggg gattgactgc ggcgagctga 9840 ttatgaccga tacgacgtat tcatgcgaca tgctggacat attcggagat gagatatacc 9900 atgtggagag gcttgacgca ttcaacggca tggtcatctc cttggacggc cccctcgacg 9960 ggacggtcag tgtatcgctt cgtgacaacc acggcatccc gctggcgcag catcggctgc 10020 ataaatacga gattttgatt ttggacgccg ctgaaaacag acccctgtca gtctcgacgg 10080 accccaagcc cgtggaggat ccatcgcccg tgcagcatat agagtccctc cagatggatc 10140 cggagcccgt ggagtccgag cccctcccga tggactccga gcccgtggag gatctggaac 10200 ctgtgcagca tctagagtcc ctcccgatgg accccgagcc cgtggaggat ctggaacctg 10260 tgcagcatct cgagcccgtg cagggatccc cgcccgtgca gggagggccg gagtccgtgg 10320 agtcaggcat agcatacacg ctatggcagt tcctttcagg actgctggat gccctgggtc 10380 ttgccgaccc ggatgtcgga tctgtccaaa aaacgtcctg atgcgttcaa aaagccggcc 10440 ctgcccccgt gtgcgcggcc atgcttcaac atgatgggtt tgaagggacc agcccgcggt 10500 cctgccggca tccaattccc gagatcctgt tacgtgcatc ggccatccct gtacgctgcc 10560 acatggttac tttgtgtgat catttccggg cagagatcaa gtcattgatt ggaaaactta 10620 aatcatgcat gggatcgagg gcggccgggg agatatgtcg gagaattttg tggcgttttg 10680 cgtggcgtgc gccaggggag tcacaaagga cgagatgaag tatgtagacg ggagggtctt 10740 ccacaaagag tgccatgcaa ggcacggcgg gcagatccgc ttccccaacc cagaggtcga 10800 gcagcgcgtg gccgagctga aggtggacct gatacagatg agaaaccagc tggccgagat 10860 gaacagggcg tcgggggacg gaggggtgca ttccagcgcc acctctgcgg ccgaggccga 10920 gcagcacagg gccgagctaa aggtacagct ggtgcagatg agaaaccagc tggccgagat 10980 gaacaggaag gcccccggaa agccggcacg gaaaaaggcc gcaggcaaga ctgcacggag 11040 aaagagcggc aagaagacgg tgcgcaggaa gaccggcaag aggactgccg gtaagaaggc 11100 cggggcgcgg aggaagacta cggtcaagag gacggcgcgg aggaagacca cggcaaagaa 11160 ggcagccggc agaaaggccg gggcgcgcag aaaggccaca gtcaagagga cggtgcacaa 11220 aaagattgga gtgcggagga agactacggc aaggaggacg gccggtaaga gtacggtgcg 11280 caggaagagc acagtcaaga ggacggtgca caggaagacc ggcaagaagg cagtagtacg 11340 caggaagagc acagtcaaga ggacggcacg gaggccggcc ggcagaaaga cccccggaag 11400 ggccgcgcgc agggccggcg caaagaggcg ctagcctgct gattaggaat ttaaggcggg 11460 cgccgggcag caggtaaatg cagtcgcttg gacggctaga cgaggcgtgc gcggagatat 11520 cgcgcagcct gcttgaatac gagtccccca ccgccggtga tgtccggacg gagatcagaa 11580 gggcatgcac aaagtactcg ctccggagga tcccaaagaa ccgcgagata ctggccaccg 11640 ccaggggtca ggactttgac aggctgcgcc ccctgctgct caaaaagccc gtaaagaccg 11700 catccggggt ggccgtgata gcagtcatgc ccatgccgta cgcgtgcccc cacggcagat 11760 gcacatactg ccccggcggg gaggcgtcga acacacccaa cagctatacc ggcggcgagc 11820 ccatagcggc gggcgccatg aacagcgggt acgacccgga agagcaggtc cgcgcgggtc 11880 tggcccggct gcgcgcgcac ggccacgatg tagccaagct ggagatagta atagtgggcg 11940 gcacattcct gttcatgccg caggagtacc aggagtggtt cgtcaagtcc tgttatgacg 12000 cgctcaacgg gtccgcttcc gcggggatgg aggaggccaa gcaccgaaat gaaactgccg 12060 tgcacagaaa cgtgggcctc accatagaga ccaagccgga ctattgcagg acagagcatg 12120 tggacgcgat gctcggcttt ggggccacgc gcgtggagat aggcgtgcag agcctccggg 12180 aggaggtcta cttgagggtc aaccgggggc acggctacca ggatgtgaca gagtcgtttg 12240 ccgccgccag ggatgcaggc tacaaggtgg ctgcccacat gatgccagga ctcccggggg 12300 ccaccccgga aggcgacatc gaggatctgc gcatgctgtt tgaggatccc gcgctcaggc 12360 cggacatgct caaggtgtac cccgcgctag tagtaagggg cacccccatg tatgaggagt 12420 attcgagggg cgagtattcc ccgtatacgg aagaggaggt catccgggtg ctctccgagg 12480 ccaaggcgcg cgtgcccagg tgggcgagga taatgcgcgt gcagcgcgag atacaccccg 12540 acgagatagt ggccgggccg aggagcggca acctccgcca gctggtgcac aagaggctcc 12600 aagagcaggg ccgccgatgc cgctgcatac ggtgcaggga ggcggggctc gcggggagga 12660 ccgtgccgca gaagctccgt attgacaggg cggactattc ggcctcgggg gggagagaat 12720 cgtttatctc gcttgtagac ggggatgatg ccatctatgg ctttgtgcgc ctgcgcaagc 12780 cctccggagc agcacacagg ccggaggtca caccggaatc ctgcataata cgcgagctgc 12840 acgtatacgg caggtcgctt ggcctcggcg agaggggcgg catacagcac tcgggtctag 12900 gcagaaggct cgtctcagaa gcagagtctg ccgcccgtga gcttggcgcg ggcaggctcc 12960 ttgtgataag cgccgtcggg acaaggggtt actatcgcag gctcggatat tcacgcacgg 13020 gcccctacat ggggaaggtg ctctgatgga gacgataggc cgcggcacct ggatagacaa 13080 gctggcgcat gaactggtag agcgcgaaga ggccctcggc cgggatacag agatgataaa 13140 cgtcgagagc ggccttggcg cgtccgggat accccacatg gggagcctcg gggatgcagt 13200 cagggcgtac ggcgtggggc tcgccgtcgg cgacatgggg cacagcttcc ggctcatagc 13260 gtactttgac gacctcgacg ggctccgcaa ggtccccgag ggcatgccat cctcgctaga 13320 agagcacata gcccgtcccg tctcggcgat acccgacccc tacgggtgcc acgattccta 13380 cggcatgcac atgagcggcc tgctgctaga ggggctcgac gcactgggca tagagtatga 13440 ctttaggcgg gcaagggaca cgtaccgcga cggcctgctc gcagaacaga tccacaggat 13500 actatcgaac agctcggtaa taggggagaa gatagccgag atggtgggcc aggaaaagtt 13560 tcgcagcagc ctgccgtact ttgcagtctg tgaacagtgc gggaagatgt acacggccga 13620 gtccgttgaa tacctggcag acagccgcaa ggtgcggtac aggtgcggcg acgccgaggt 13680 aggcggaaga aagatcgccg gctgcgggca cgagggcgag gcggacacgg gcggagccgg 13740 cggcaagctc gcctggaagg tggagtttgc cgcaaggtgg caggcgtttg atgtacgctt 13800 tgaggcatac ggcaaggaca tcatggactc tgtaaggata aacgactggg tctccgacga 13860 gatactatcc agcccgcacc cccaccatac aaggtacgag atgttcctcg acaagggcgg 13920 caaaaagata tcaaagtcgt caggaaacgt ggtcacgccg cagaaatggc tcaggtacgg 13980 caccccccag tcgatactgc tcctcatgta caagcgcatc acgggggcgc gggagcttgg 14040 cctcgaggat gtgccatccc tgatggacga gtacggcgat cttcagcgcg agtactttgc 14100 gggagggggc aggggcggga aagcccgcga ggccaagaac agggggctat tcgagtatac 14160 gaacctgctg gaggcacagg aggggccgcg gccgcatgcg ggctaccggc tgctagtcga 14220 gctctccagg ctgttcaggg agaataggac cgagcgcgtc acaaaaaagc tcgtcgagta 14280 cggggtaatt gacgggccct cgcccgggat cgagcggctc atagcactgg ccggaaacta 14340 tgcagacgac atgtattctg ccgagagaac agaggtggag cttgacgggg ccacaagggg 14400 ggccctctcg gagctggcag aaatgctcgg ttccgccccg gagggcggac tgcaggatgt 14460 catatacggc gtggccaagt cccacggggt gcccccgcgc gactttttca aggcgctgta 14520 caggataata ctggatgcat ccagcgggcc gaggataggc cccttcatag aggacatagg 14580 cagggagaag gtggcaggta tgatacgggg gcgcctctga tggtccacga cgtacacaac 14640 cggcggcgga gcggcggctt tttcctgata atactgggcg ccctcatctt catcacggcc 14700 ccgctgtacc tctcagattc gccggagctt ggggcggcag ccatagcctt tggctttgtc 14760 gtgggcgggg cggggttcta tctcaacttt atcaaaaaga aatcctaggg ggcccggccc 14820 aagcatttta tggcaagccc ctgtgggagc acccatggga ttgttcagga aaaagagccc 14880 cgaagaatcc gagccggggc cggacgagcc cgggcccgag gcggagatgg aaaaggtgag 14940 ggcggacctt gccggcgtgc agagagacgt ggtcaaaaag tattccgagc tgacggccct 15000 ctcagagaag ctcgagaggg taaagacaga gtatgattcc accgtgggct cgctgatgtc 15060 cgagagaaag gggctcgccg aggcgaaaaa agagtccgca tcgctcgagg aggcgcgcgc 15120 aggcctcgcc gaagaagtcg agcagaagag ggcaaaactc gaccaggcgg agattgacct 15180 tggggataga aagggcagga tagaggagct cgaccgcgcc cacgcggctc tcgccgggat 15240 aaaggaggag tcagacaggg gccgcgccga gctgcacgag atcaagcgga agatcctcga 15300 gtcacagggc gcgctcgaca gggccaggga cgcgcaggcc aaggcggaag cggagctgca 15360 caattccgcc gaggggctca agtccgcccg ggacgaggcc ggaaagctct cccaggagcg 15420 cgacaatata cgggccgaga tagatcttgc aaaaaaggag ctaaaggtgg tccggggcca 15480 gatggagtcg tcccccgagg ggggcgccga aaagcacgtg gtcgaggccg caagcgcgat 15540 ggtctcgtcg ctcacccaga ggctggctgc cgccgagagg gagcttggcg tggtaaagaa 15600 ggtattggag agggagagaa gggggcgcgg tcaggattcc gaatagatct ttttccactt 15660 gctcttcatt tcttcctcgc tcattcccag atcgtacagg ggtgatgtga tatcgcggat 15720 ttcatccacc gttatcttta ccacggaaga tatcccgctc tttatgccgg tctttttaaa 15780 atgggacgcc atcgtatcga actcttcgcc tgaatcgagc acggcggcag tgccggtaaa 15840 gcggtagccc tttctgagca gcgggtctat aacgttgatc tctatggcgg ggttggcgcg 15900 caggttctcg acggtattgg gtgaccttat gttggcaaac gccaggtgcc cctcgtccca 15960 tacgatcaca gagcccttgg gcgagaggtt cggcttgttg tccggggtta cagtggccac 16020 aaagcccatc ttgatgcggt tgacgtgatc ccttacctct gttccgatgg tcgccatggg 16080 ctatccggtc atgcggggtt aaataaagct ggggtgcagc cgcagacact cagccgttga 16140 ttatacagta ctcgcactcg caggtcttgc tctcgtcatc ttttgccctg cattttgcgt 16200 gctggcccga ctggcactgg acgcagatct cgtctatgtt gtctatcgag gtgtatttct 16260 tggactggtc aaagccgaag ccaccgtcct ttggtccaac catacagcgg ttatgccgcc 16320 gtaccttaat tatctttgcc gtcggggcgg ggccgcttct gccggcggga gccccccttg 16380 aggccgcccc ccggctcgtt gtcccacaga tgcatgctgc cccggatcat aaacgagccg 16440 actatgatcg ccacaagccc gcccaccacc agtatgaaca gccttgccag gcccgccaac 16500 ccgcccatgc gtgtaatatg cggcacccgt aaacaaacat tgcgcgaaga aacaggggta 16560 ttccaggggc caaggcccgc aagctacatt aatttatcag tccggagggg cgggccccat 16620 gagcagagtc aaggcgatag ccatctgggt ggccatgata ggcggaggca tagccttctt 16680 tttcgcctcg gactatgttg tagaagtaat gaggtaaacg gggaaaacag gatccaatac 16740 ggtttcttat cctccctgtc catggcggcc ccgttcatat aaagtcccaa cgttcaatgt 16800 gccagatacg atggtccctc acaggggaat ttggactgtc ccgtgatgtt ggtggtttag 16860 gaaacgcatt acctaggcat ttatgtagtt gaggtgcacg tccgagggat ctgtttcatt 16920 atctggatca aataatggaa cttttctctt cattaatttc ctacattccg cctgcagttc 16980 aagcatgatg gttacatcat cacgttcgat tatccgttct gttgtttcgg ttttttgctc 17040 actcccatcc atgccaatat acgacggcat ttgtctaaaa aggtttccat cttcaagagt 17100 cgtgtgtttg ccgtgcaatt acctcccagg ggagcataaa aaataagcca taaacgatgc 17160 acagcatccg ccatggacct atgaaaaaac aagtcgtcag tggcgcacag tatccgatac 17220 aacctctcaa tcgtgtcatc cggcaactct gcaccatgga aactccggac gatcgctatt 17280 ctatacatgc tggttatcgt acctgtgaga atcctaatca tcctacggct atgttgatgg 17340 tttgctgata actgaattac cgaggtgatt cgcgatgatt ttgttgattg gagtaaattg 17400 agacaggata tccgctgcat gagataccga caatgtcaga tcctgaaatt cttggtcggc 17460 ttctagatca gttattttca aaccgatgcc cctacactcc tctcgcgata tgtatctccc 17520 atgactgtaa tattttccag gagaagctaa cattccagat attttttttg atttttctgc 17580 cgcatcagac tcgccagcaa acatgtggtc ttccagccat ttttgtacaa gcacttcagc 17640 tagtttctgg ctgctaatgc atttttgaac cagtccagga ggatattgtc ctaacaatgg 17700 aagccatgcg ccaagcctgc ccggatgttt ttcagatacc acctgcactt cttgcaactc 17760 gtcaattaga agctgtgcag acattatttg catgccaatc ttggttggga aaataaattg 17820 gggatcagcg ggtcctatag acgagtgttt gcccattacc agggaatttg atgcgcaagc 17880 aagcatcgag gctgctgaca ttgcggcata cggtatgatg acccgaatat catcatattt 17940 cgcatgaagg tatgtgacaa tcgattctgc agactcggca gaacctccag gactgtggag 18000 tatcagatcc aattttttag tctttaaatc acgcatcatc ctcataaatc catacaggtc 18060 accatttgtt atgagagctt cattaggcgt atgcggttcg tccgtcatcc agttggtcgc 18120 atatagaatt gtgtccctcc ccgaatactg ttgcaatttt gaaagatagt cgtgaagtac 18180 cacaccaggt gcctgctcac cggctgactc cattagtcga agtactttgt ttgatatttc 18240 tgcgtagcta ggcatttatg tagttgaagt ggctgccggc gggaattaac ccatagtctg 18300 aatcgtatga cttgcctttt ttcttcatca atttctcata tacctcatgc aggtctagca 18360 ggatggccat gtcactgcgc ttgcttggta attttgttgt ctcgggcttt tggtcgatat 18420 tcttctccat gccgataaaa gtcggtgttt ctttaaaaac gttcatgatt ttaattttcg 18480 agtctttgcc gtgcaatttc ctccagagat cataaaaaat gagtcataaa cggtgcacag 18540 catccgctgt ggacccatga aatgggcccc cggcggtgca cagcatccgc tgggggctca 18600 ataaaaaaaa tgagtcatca tgcatagtct ctatgtaaat ggctgaaccg gtgttttggt 18660 cgattagtaa aggctggctc tccactcgcc gaagcttgtg ggttacacca ccttcctatc 18720 aacgcagtct tcttctgcga accttcatcc gaagaaggaa tatcttgtct cgggatagga 18780 ttcgtgctta gatgctttca gcacttagcc tagatggctt agctgcccgg cctgccctgt 18840 cggacaaccg gtagaccagt ggccacgcct ctctgttcct ctcgtactag gagcgacttc 18900 ccctcagata ttcgcgcttc catcaggcag aggccgacct gtctcacgac ggtctaaacc 18960 cagctcatgt tcccttttaa taggcgagca gcctcaccct tggcccctgc tgcaggacca 19020 ggataggaaa agccgacatc gaggtaccaa accgcggggt cgataggagc tctcgcccgc 19080 gacgagcctg ttatccctgg ggtaattttt ctgtcacctc cgggccccaa tagtgggcac 19140 acgaaggatc gctaagccag actttcgtct atgaattccg tgcgtttgga aatccattca 19200 gtctagtttt tggctttgcc ctcttcagcg gatttctgac ccgcttgaac taaactttgg 19260 gcccctttga tatcttttca aaggggtgcc gccccagccg aactgcccac ctgcacgtgt 19320 ccccggtctt caccgggtaa gtggcactgc aggaaatgtc tggtgttaca tcggcgtccc 19380 ctgacgtccc aaagaacgcc aggaaaagac tcccagatac gctatgcact ccctgctata 19440 ccacaagcac aagctgcagt aaaactccac ggggtcttct ctccccgatg gaagatgatg 19500 gactgttcgt ccaccttatg tggcttcacc gggttgtagg cggggacagt ggggctctcg 19560 ttgttccatt catgcacgtc ggaacttacc cgacaaggca tttggctacc ttaagagagt 19620 cagagttact cccggcgtta accggtcctt agctcggttg aacccaagtt ttagataccg 19680 gcaccggcca ggattcagcg actatacata ccctttcggg ctagcagtcg cctgtgtttt 19740 tattaaacag tcgaaacccc cttgtcactg caacctgctg ccgccattcc tcatgacagc 19800 tgcaggcatc ccttatacct aagctacagg actaatttgc cgaattccct cgccatacgg 19860 tatacccgta gcaccttagt ttactaaacc agcgcacctg tgtcggatct gggtacgaac 19920 ttgcagtttg ctagccgcac ggtctttcat ggtctcctgg agtcggggga actctgctaa 19980 cgcaaagcca ctcccgcctc gggcctgttc tcgtcattac gacactccca ggccctcgaa 20040 cggttcgaca cgacgacggt catgctcccc ctatccggaa gcgaaccatg cggttcaaat 20100 gctccctgca aggtaccaga atattaactg gtttcccatt cggactactc tgttgaggca 20160 gcccttagga tcgactaact ccaggctgac gacgcattgc ctggaaaccc ttgcgctttc 20220 ggtggtgcgg attctcaccg cactatgctg ttactgccgc caggatctgc aatagaaatc 20280 ggtccacagg acgtcaccgc cctgcttcgt cccaatcact acgccaacct accacggtgc 20340 acctgccacg gtgcacgtcc ggagtatcgg tactctgctt tagccccgtc cgtttttgtg 20400 gcgccctcgc tcggcaggta agttgttaca cactttttga aggatagcta cttctgagct 20460 tacctccctg ctgtcttggc gacgacacgc actttggctt gacacttagc agaaatttgg 20520 ggaccttaac tccagtctgg gttaaacccc tctcggtcgt gaaccttacg tcacacgaac 20580 ccgtgtccat gcttctgcga tgtgtatccg ttcggagttt gaatggatgg tgaggaatct 20640 cttccccgcg ccaccctatc agtgctctac cggaaacacc atctccacat agcacgccct 20700 gcgagacgct tcggttggaa ctagcaagcg ccagtctaga ttggtttttg acccctattc 20760 ccaagtcaca caaacgagtt gcacgtcaga actgctgcag acctccagtg ggctttcgcc 20820 caccttcatc ttgctcagga atagatcgac tggcttctag ccttaccgcc atgactcaac 20880 gcactttcac acgcttctcc tcacaatgct gcgagaattc ggtttccctt cggctacgcc 20940 tttctaggct taacctcgcc atgacagcaa gctccctggc ccgtgtttcg agacggaacg 21000 cacgacactg acgacatgag ctccggactt ttagctccat tgctggaacc tccggtccga 21060 aaaaatcgtc tttcatgccg tgcacgtctg taagcaatag gtttcatgca cttttcaccc 21120 cccttccggg gtacttttca gctttccctc acggtactag tacactatcg gtcttgagag 21180 atatttagcc tttgatgcta ctttcaccaa tcttcgctgc ccactgccaa ggacaactac 21240 tcgggtgctg gccctgcccc attccacttc gtctaggggg gtatcaccct ctaagccgga 21300 acatttcaga acactttgac tatttcgtgg ggccattgcg ccgcaccaaa acaccacatc 21360 tcggccgcgt taccgcggca gattcagttt gggctctttc cttttcgatc gcctctactt 21420 gggaaatctc tattgatttc tcttcctcgt ggtactaaga tgcttcaatt cccacggttc 21480 gacctccgct tgcgcggagt atacaggatt cctattcgga aatctcggga tcaacgggtg 21540 cgtgcaccta ccccgagctt atcgcagctt gccacgtcct tcctctctcc tcaagcctag 21600 caatcctcct attgccgtct ttacaccggc atattcagcc acatattaca cgactatgca 21660 tgatgatcat cgcggtcccc aggggagggg cccgctacat ccttcatacg ccactttcgt 21720 gacgcattgc accatgtgaa gatatgtgca ccccgttcaa accagtttct aaggaggtga 21780 tccgaccgca ggttccccta cggtcacctt gttacgactt ttcccttgtc gcttacctca 21840 agttcgataa cgccaattag acgtcacctc actaaaagca aacttcaatg aaacgacggg 21900 cggtgtgtgc aaggagcagg gacgtattca ctgcgcggta atgacgcgcg gttactaggg 21960 attccagatt cgtgagggcg agttgcagcc ctcagtcata actgtggtag cgtttgggga 22020 ttacctcctc ctttcggata tggaacccat tgtcactacc attgcagccc gcgtgtggcc 22080 ccagagtttc ggggcatact gacctgccgt ggccctttcc ttcctccgca ttaactgcgg 22140 cggtcccgct aattcgcccc actgctcctg agagcaatgg tggcaactag aggcaaggat 22200 ctcgctcgtt acctgactta acaggacatc tcacggcacg agctggcgac ggccatgcac 22260 cacctctcag cttgtctggt aaagtcttca gcttgacctt cacactgctg tctctccggg 22320 taagatttca ggcgttgact ccaattgaac cgcaggcttc accccttgtg gtgctccccc 22380 gccaattcct ttaagtttca tacttgcgta cgtacttccc aggcggcaaa cttaacggct 22440 tccctgcggc actgcactgg ctcttacgcc aatgcatcac cgagtttgca ttgtttacag 22500 ctgggactac ccgggtatct aatccggttt gctcccccag ctttcatccc tcaccgtcgg 22560 acgtgttcta gtagaccgcc ttcgccacag ggggtcatca atagatcaaa ggattttacc 22620 ccttcctact gagtaccgtc tacctctccc actccctagc cgtgcagtat ttccggcagc 22680 ctatgcgttg agcgcataga tttaaccgaa aacttacacg gcaggctacg gatgctttag 22740 gcccaataat cctcctgacc acttgaggtg ctggttttac cgcggcggct gacaccagaa 22800 cttgcccacc ccttattcgc cggtggtttt aagaccggta aaagatttct ttagcagaaa 22860 acactcggat taaccttgtc gtgctttcgc acattgcaaa gttttctcgc ctgctgcgcc 22920 ccatagggcc tgggtccgtg tctcagtacc catctccggg cctctcctct cagagcccgt 22980 atctgttata gccttggtgg gccattacct caccaacaag ctgatagacc gcagtcccat 23040 cctacggcga taaatcattt gggccacaaa ccattccagg catggtggcc tatcgggtat 23100 tattctcagt ttcccgaggt tatccccgtc cataggttag attgactacg tgttactgag 23160 ccgtctgcct tgtattgcta caatgactcg catggcttag tatcaatccg atagcagtca 23220 ggtccggcag gatcaaccgg attcttattt ggattatttt ttttttcaaa gtacgcctgt 23280 acttttggaa ttgaacggaa tgcacataat cttcacatct cagatatgac ccttcgatca 23340 gatcctcatt ctgtgtgcgt aactggaggc ctgcgaatca caaaatggta caataccatg 23400 gcttcatcgc aagcgccgct cttgcgtcac gtacgatcgg atcgccttgt ccatgggcat 23460 ataaaccatc gccggtttcc gggcccgatc ggacccttga tcggcccgcg gggggcgatc 23520 cggcctcatt aaattacggg gggtacaacc tgctgccgtg gatctagagc gcgagtacag 23580 ggcaaagacc aggggctcgg cggggatatt tgcccggtcg agaaggtacc atgtaggggg 23640 ggtcagccac aacataaggt actatgagcc gtacccgttt gttacaaggt cggcgcgcgg 23700 caagcacctt gtggacgtcg acgggaacaa gtataccgac tattggatgg ggcactggag 23760 cctgatactc ggccacgcgc cggcgcaagt aaggtcggca gtggaggggc agctgcgccg 23820 cggctggata cacgggaccg caaacgagcc caccatgcgg ctctcggaga tcatacgcgg 23880 ggcggtaaag gcggcagaga agataaggta tgttacatcc ggcacggagg ccgtcatgta 23940 tgcggcaagg atggcgcgcg cacgcacggg aaaaaaagtg atagcaaagg tcgacggcgg 24000 ctggcacgga tacgcgtcgg ggctgctaaa gtcggtcaac tggccgtacg atgtgcccga 24060 gagcgggggg ctcgtcgacg aggagcacac cgtgtccatc ccgtacaaca atctggaggg 24120 atccctggag gcgctaaggc gcgcaggggg cgaccttgca tgtgtcatag tcgagccgat 24180 gcttggcggc ggcggctgca taccggcaga accggactat ctccgcggca tacaggagtt 24240 tgtgcattcg aagggtgcac tgttcattct cgacgagata gtcacggggt tccggttcga 24300 ctttggctgc gcgtacaaga aaatggggct ggaccccgac gtggtggcgc tgggaaagat 24360 agtcgggggc ggattcccca taggtgtggt gtgcggcaag gacgaggtga tgtgcatctc 24420 cgataccggc gcgcatgcaa gaaccgagag ggcgtacatt ggcggcggca ccttttctgc 24480 aaaccccgcg acgatgactg cgggtgccgc ggcactcggt gcactcaggg agagaagggg 24540 cacactatac cccagaataa actccatggg ggacgacgca agggcgcggc tctcgaggat 24600 attcgacggc agggttgcag tgaccggcag gggctcgctg ttcatgacgc actttacacc 24660 ggatggggcc cgcaggatat ccagcgcggc agatgctgcc gcctgcgatg tgcatctgct 24720 gcacaggtac cacctggaca tgattacaag ggacggcata ttctttctgc caggcaagct 24780 gggggccata tctgccgccc actcaagggc ggaccttggg gccatgtatt cggcgtctga 24840 gcgctttgcg gggggactgt gagttatacc catgggaaac tttgattata cgggcgtaca 24900 ttcccggggc ccatgatact cttcggcaag agcgacccct ccgacctgct ccgccaggcc 24960 gatcttttgt gcagtgggaa caagtacaag gcggcagtgg gcctgtacag caggatactc 25020 aaggacgacc cgcagaacag gatggtcctg cagagaaagg gcctcgccct caacaggata 25080 agaaggtact ctgatgccat aacgtgcttt gatctgctgc tcgagctgga tgatggcgac 25140 gcgcctgcat acaacaacaa ggccatagcc caggccgagc tgggcgatac ggcatccgcc 25200 ctggagaact atggcagggc catcgaagcc agccccaggt acgcgccggc gtactttaac 25260 agggccgtcc tgctcgacag gctcggcgag cacgaagacg cgctgccgga cctcgacaag 25320 gcgacaaggc tggacaggga caaggccaac ccgaggttct acaaggggat agtcctggga 25380 aagatgggcc ggcatgcaga ggcgctgtcc tgcttcaagg aggtgtgcag ggcggaccac 25440 ggccacgccg actcacagtt ccacgtggcg atagaggtag ccgagctcgg caaacacgcc 25500 gaagccctcg gtgagcttgc ggcactgccc gcagagtacc gcgagaacgc aaacgttctc 25560 tacgcccggg cgcgcagcct cgccggcctg gacaggtacg acgagtccat tgcacacctg 25620 caaaaggccg ccagaaagga ctccaagaca ataaaaaagt gggcccgcgc cgagaaggcc 25680 tttgatcata tacgggatga tcccaggttc aaaaagatag ccgggtaaac cctacagcat 25740 cttttttctt gccgcgtcta tccgcattat ccggaccttt tttttgggcc ccacaagccg 25800 cgactcgtag acaggggcat acacttcttc gaccgatctg actgcaaact cctcccggag 25860 gtcgcgcatg ccgtcaggcg ggggcccgcg gagctttacc cggagttttt ccagcgccac 25920 cccggtgtct atctccgggc gccgcacatt ctccgacgaa tcaagcatgc gccgcgggta 25980 cggctcgacc gcgccggtcc ccgtcttgta gggaaagccg gtctccccgc cgtgccggtc 26040 aaggcacatc acgccctctg attccgcgta tacgtgctcc tcgagttcca gatcgaccct 26100 gttcttgccg cgcccggcgg acagggtctt gcgtatgcgc gactttgacc ttatcgggaa 26160 gacgccgtcg cccagcacca cctcgatcac gttctgatcc accttgatcg ggtgcacggc 26220 ccttctgaaa aagtcggccg agtaccgggc ggagacgcgg atgagcgcct cgtggacgag 26280 ccttacggaa tgtacatgga cgtcctcctt tgggggcgcc ctcatcatgg ccctgaatgg 26340 ccccgtcttt tttgcctcga tcatggcccg ggcggactgt tcagtcatgg cgttgcgcag 26400 gacgatgatc cttgtcttgt cagtcactcc cggcaggccc cctcatccgg catgccctgt 26460 acacacggca aggtaataat agcctgccgt ccgtacctgc cgtatgaggt cagaagagag 26520 gccgggtcac attgaaaagt tcctaaagag ggcggacaag gcgatcgaca gcgcggtcga 26580 gcagggcgtc aagagggccg acgagatact agacgatgca gtcgagctcg gcaagattac 26640 ggtgggcgag gcgcagagga ggagcgatgt gctgctcaaa caggccgagc gggagagcag 26700 gcggctcaag tccaagggcg ccaaaaagct cgaaaagggc ataggcgccg caaaaaagat 26760 ggcagcaggc aagggcgacg cgctcgagac gctcgcaaag ctcggcgagc tcagaaaggc 26820 ggggatcata acggagaaag agtttcgcgc caaaaagaaa aagctcctcg cagagatctg 26880 acatgaaggc cataatctac tcccgggacg gctccgcaaa ggaggtcaca aagaggtggt 26940 ttgtcggtac tccttcactg atgaaccttg caggcgacct tggcatgacc gagagtgaca 27000 tattccatgt gacatttccc gacggcgcca agacgaccct gcacacacac gaaggcgggc 27060 agctgctgat agtcacctcc ggaacgggca gcatgtcggt ctttgaaaag accggcggcg 27120 gggataccga ctttgcgata aaagagaccg accgcatacc gctaaaggag ggcagcatac 27180 agtacatacc ggcgggtaca ttgcacgtgc acggcgccat cgagggcacc accctctccc 27240 acatagcggt aaactatccc tccccgtcgg gaaaggagcc gtataccatc tggtacgaat 27300 ccgactttgc gaacagggtc accggcgtgc tataagctac tttagccgct ccagtatgga 27360 caggctcaca aggttggtca tcgttatccc cttgcggatg acctcgcgcc gcgtcttctc 27420 gcagtacttg ttgacgctct ggtagctctt ctcgctggag acctcgagga ctattatgtt 27480 cccgctgtac ggaaaggcaa actttggcgg caccctcgag cagacgtcca tgcgccctat 27540 tcccgccctg cctatcttct cctttctccc caccacggac gccacctcgc atatgtcctc 27600 ctcggaatcg ccgtactcca gatagtacac cgatacatag ctcaccccgg gggactccct 27660 ctcgaatatg tcctcgtcca tgctaaacac ataggatgcg ccctcccccc ggcccaccgc 27720 cttgatcatt ctgggggcgt ccttgaacct tgccagcagg tagtggttgg cctgcggtgc 27780 aaagtacggc tggctctcgg acgagaacgt ggcggtcaca aagtacatct cgcggacgtt 27840 cctcgacgag gcccacgcgt ccttgagctc gatcgaggcg tgatcgtgcg tgtatggcgt 27900 gcaggcatgc ccgcccgggg cccccgtctg ggacatgcat tgtacccgcg ggaccggtta 27960 ttatctagtt ccatgggggc gcagggcgcc gcccccgtgt ggcatgcgtg gatccatgcg 28020 atagttattt aaaactagga tgccgggcac ccgtcgtccc aagctagctc agcctggtag 28080 agcttccggc tgtagatgtc ggccttggct gaccgtataa cagcatatca ggcatacaga 28140 gaccgggttg tcgaaggttc aactccttcg cttgggacca cattataacg gctgccgcct 28200 catgcggctg tgcacggcat ccgtacacgt tccatgcacg ggtgccgcgg cgtgccatat 28260 gcatggatgg tgcatgtaca atgcacgggt gccgcgacgc actatacgca tggatggtgc 28320 actatagatg cggctaaatg tgcacggcag agccgcaggg cccgggccgc gtgcacctat 28380 attctgccct gtcccagggt caggagccgc gtcgccagaa cgatgtgcgg cgcccgcgcc 28440 gcacggcggg gccgccgggg gcgcacgaca ccgcatcgcc ggacctggcg ccatttctct 28500 ccagcagtgc gtcgagcgca gaatcgtcga ctagcgtgcg ggatggcagc ccgcccgggg 28560 taggcacgcc ctgcagctcc attgcccggc gcatctcctc gttctccatg cggaggcggc 28620 gctccttctc ctcgagcctg ctgctcatcc tgtcgaggaa cattacatct gaattgtaca 28680 gctccctttg ctgccccatc atgcagagga tatggcgcag ccttgacgta tcgcatatac 28740 cggatgccat gagctccctg accccgtcct ccgtgtggcc gatgtgccgg ccccccgccg 28800 atgccctgcg cacgccggac ctcatcgctg cttcctcagg tactcccgga ctatcctgtt 28860 ggcaagccgg gtctcgtcgg tgccctcgcg ctcggccacc cgggcaagct ttcttgcgcc 28920 gttcttgccg agctctatgg ggatcttctt cctgacgccc gtcttgcgca ccctctttag 28980 caggatcctg tggcccgagc gggggctctg ggctagcagc ctcaggtaga tccgcctcga 29040 cggccgatca agccttgata tgttcagcgc caccttgagc gcctgggaga cggtcgggac 29100 ggcctggtac agctttgtcg cctcgtcccg ggatatggtc ccggggacta gcgccttgat 29160 cttctccggc acgcccgcaa agccgtggta ctttttgaac gtgggcatcg acatgccgag 29220 cttccttgcg gcctcggcgc gggtcatctg ctcggcgaga aacctgcacg cgtcggcgag 29280 ctcccggggg ctcatctgca tccggtgcag gttctccacg accgatgccg cctttgcgtc 29340 ctccaggccg tactccgtat ccttggttat cacaagaaac ttggactttt ttgcgcccag 29400 atgctttagg gccgcaagcc tgtggttccc cgatatgagc aggtacagcc ccctgccgcc 29460 cctctgtatt acgggcgggt tctgcaggcc ctcggacctg atcgactttg caatctccct 29520 caccctggac ctgtccagcc tccttgcctg cgcgtccttc cacacgtgca cgttcttgag 29580 gggcacctcg cgtaggacct gctttatccg gggcttgtag cgccgagcca acgtactcta 29640 caagatacaa atccttgtta actgtgtttg gtaagtttat cacaacaatt aggttagata 29700 gagctgttcc cccgcaggcc cccgtgcacg tactctatcg cgcagccccc cgggggacag 29760 ccggaaccgg gggctgccgg ggcgggatcc cgggcgtcga tagaataaat acgcgcgggg 29820 ccgcggtgcg atcgcccgtg ctgataataa actgcaaaaa ctatgaggag gccgccggcg 29880 gcaggatccg cgggctggca gatgccgcgg ccggggctgc cgccaggtac ggcgtcagga 29940 tagcgatagc cccgccgcag cacctgctgg gcattatagc aggccgggat cttggcgtgc 30000 tggcccagca tgtcgacgac aaggggacgg ggagcaccac agggtatgtc gtcccggagc 30060 tgctaaaaca gtcgggggtc tccggggcca taatcaacca cagcgagcac cgcgtacccg 30120 cggaccaggt ggcgggcctg gtaccaaggc tcaggggcct tggcatggtc tcggtggtct 30180 gcgtcaggga tcccgccgag gccgccgatc tctcccggta ttgccccgac tacatagcga 30240 tagagcctcc cgagctgata ggttccggca ggtccgtctc gacagagagg ccccaggtca 30300 tacaagaggc cgcagaggcc atcagggggg ctggcggcgt aaagctgctc tgcggggcgg 30360 gcataacctc cggggcggac gtgcgcaggg ccctcgagct tggctccgag ggcattcttg 30420 tggcaagcgg ggtcgtaaag tcggcagacc ccgcaggggc catcggggag cttgcccggg 30480 ccatgtcctg acgcaccatt ctaggcgccc gcgccgttga ggcgcgccag caggtcaaac 30540 gacgacctgt atagctcgcc tggcgaccgg gcgcccgcta tcaccacctt tcccgacgca 30600 aacacaagga agctgcagct gcccagcccc tttagtatca tgccgggaaa cgaccccggg 30660 tcgtacaccg cgccgggtat ccgcgacgat atcctgtcta tgggaacagt ccgtcctgca 30720 tccactgtcg ccaccatatt gcgtacgacg ggccttgtac acccgccggc cgccgccccg 30780 ttccggaaca ggtgcagccg ggcctcgtgc agctgcgcaa acgatgccct cacggagctg 30840 gcgccgacgg atatcatctt gcccgagaga aacaccgtca cgcgcccccg catgccgggt 30900 gttttgatat agccgcacct gccgccgtat accgcctcgt cgtacatgca gcatggcatg 30960 gcggccatct tttttgcgcc caccctccgg cccaggtcgg cggtactcac aacgttgacc 31020 accctgggcc gtttccttgg atccagcatt gatccggtgc ccggccgcac tctattttaa 31080 ccggggcccg ggcggcagcc ccgcggccct gtcgtacctg cgctttagca gcattacggc 31140 ggccatcagc gccaccccgg ccacgttggt ccctattatg tagacgtccc atatgtgcac 31200 gccgtaggct atccagagca tggcccccgc gcctatcagc atggtcagat accacgatac 31260 atccctgagg ctctttgtcc tgtacgcctt gactatctgg tgcacccatc ccgacagtat 31320 cagcacgccg ccggcagccg ccacgatatc cagcagggcg atatccacgt tcatttgaaa 31380 aagaactgct ccatgccggt ctgctttggc ttgccgagta tctcgtcaaa gtcaaggccc 31440 atggacgagg tgagctggtc gagcgtcgac tccatgaact cgaggtactt tgacgtgtcc 31500 acctcgcctg cccgggccat ctccaccggc ttgacgccgg tcttgttcat cacctttacg 31560 tacgatatta tgtcgccctt tttgacctcc cttgcgttct ccagcagcct tgccgcccgt 31620 atgtgctgcg ggacggtctt gacatattcg gagggcgcct tgcttatcat cacattgaac 31680 gccaggtcca cgagggggat ctgcctctcc tcgagcctct tgccgcacgc ggcgatcgcc 31740 tttgagatcc tcatcttggc tgactcgaac tcgtcctcgc tctcgactcc tgagagtatg 31800 tcgagcagcg agtagaagag ctcctttatg aacgggggcg tgtgcgactt tttgcccgtc 31860 agccccttga cgtcgacctt gcctgcccgg gtcaccccga aatagttttt tttcctgttg 31920 gatagcacga catacctgta ctctttgtcc acttcgagct ccacaccgtg ctccttcttt 31980 gcatgctcga ctatctcgtg gatctgcctc tcttcgggat cctttatgaa cagagaatcg 32040 gtgtccccgt acagcaccct cactcccatc tgctcgcagt gcgatatcgt ctgcatgatg 32100 atatagcgcc cgacagcagt ggtggcctct gccgcgggta aaaagtacag cgggaatatc 32160 tcggcgccca tcacgccgta gcttgcgttg agcacgacct tgagggcctg gctgattacg 32220 gtatactgct gccgctgctc ctccgtaatg gatgtgctct ttgagaggct cttgtaatag 32280 ttgacgcgca ggtcccgcag cgagccgatt atcatcgatg tcaggccgtt gttttttgta 32340 catacccagt ggttggtatc ggggatggtg ttctttttgc attctgcatg cacgcaccgg 32400 acggtctcgt acgagaggtt cctcaccttt atgatactgg gatacaggct cgcaaagtcc 32460 atcaccgtaa catcaaagtg tatgccctct tcaggctcga cgacaaggcc cccgcggaac 32520 tttttatcct ttattaccgc gtcgttgctc acctcgcgcg acctgccctc cagctcgtcc 32580 ctccgcggta tgagcgcgtt tcgctgtctg tgctcatagt acagcaggct gcgtatccac 32640 tgcgagacgc ccatgcggga catgtcatcg atgggcatcc gggctattct gctggtcacc 32700 accagcaggt ccatgagtat ctcgttgcca aaggtgctaa gctcgagcgt caggcgcgcg 32760 tcgtgatagc aatagtttgc agtctggtat aaggtgagat cccccagttt gaccccatag 32820 tcgaccttgc cctcgccgag catcgccttt gtgacgctgt taagggaata gtccgtgtac 32880 tttgccgcaa aggcgtacag ctggaatgac ctgttcgaga aggtcctgta caggtccagg 32940 tggactccgt gccggagcgt ggcagaatcc cgcatcatgt acaaaggaat gtcagagtca 33000 gatactccga ggcgccgtgc cctgttgagc atgtacggca tgtcaaagtc gtcgccgttg 33060 tacgtcagaa caaacgggta cgagcctatt accgatagcg cgtcgcggat catgtcagct 33120 tccttgtcgt agaataccac ctcgacaccg ggggtcacgc cgttctcgcc ctcttctgcg 33180 ccgctcctca ggacgaatac ctgttttagg ccgtcggtgg cggcaaaccc caccgccgta 33240 accctcctgt cggatatctt ggggtcgggg atcctgccct cctctgaatc cacctcgata 33300 tcaaagctga ggcgccgtat cctgggtatg ggctggttga gcaggtccgc ccaccccgct 33360 atgaactcgc ggaactcttt tctgtccgcc atgccctcgt ctacaacctt gtcccagagg 33420 aggctcttga gggccagctt tacctcgtcg gatatgggca tgtcatgcgg gattaccttg 33480 ccgccggata ccgaatagta cctgcccacg accaggctct tgtcgtacag atagttctca 33540 tagtacttta tgtcggattc ccacgtgtcc atgatgttgc ggatgctctt ctccgagttg 33600 gtcccgccta tggcaagggg gtcggccaca gttatcttgg tgacgggcac atccttgtcg 33660 gctatcaggt cgtgccgcat gacctgctcc gttcctagca catcctccct gccttcaagc 33720 tccccaagct cggagggggg ctgcctcgta tagcagtagg gcttgtgccc cgtattgtcc 33780 gtccagtgta cgatcttttg tgattccggc tcgtaaaact tgaggacgac cgcccctgcc 33840 tggctgtcgt atgttgcaga taccagcagc gacgggggta tctctacggc atcttgcacc 33900 gtcaccgcac cggcacctcc ttgctgcctc cgggcatcct tgacgcccag tacgagatct 33960 tttccttgtc catcatggtt atctcggagg atgtctcttt taccagccgg gaggtgttct 34020 cggggtaggt atcaaggcag acaaaccgcc tgatccctat cgttacggcc atcttggtac 34080 actccagaca cggcgagaac gtggtgtaca tggtggcccc cccgcccccc gcgcctatcc 34140 cgagtatcgc acagtgcatt atagcgttgg cctctgcatg gttgcacagg caccggtcca 34200 gggcctcgcc tgacttgatc ctgccctcga tgcgctcggc acacctctcg cagccgccct 34260 cgtagcagtt cttgacgcca ggaggcgtcc cgttataccc tgtggcgagc tgccggtggt 34320 ccctcactat tacggccccc accttgcgga ctatacagtt ggatcggagc tttgcaagct 34380 ccgcctgcag catgaaatat tcatcccagg tagggcgctc aaagccgctc acgggcagcc 34440 tgcccccgcc cggcatatta tggtatatgc gggacggggc cgtccacccg cacccccgta 34500 tatggatctg cgatcagggg gtagaaacca taaaacaaca ggccgcggca gggcgcgcgt 34560 ggagactggg cacataacgg gcaggtacat cgagcccggt gccgtcgaga ggcgcgacta 34620 ccaggtgggc ctggcggaac aggccatacg ggagaactgt atcgtggtgc tcccgacggg 34680 cctcggcaag actgccgtcg ccctccaggt gatcgcccac tatctcgacg agggccgcgg 34740 ggcgctcttc cttgccccta caagggtcct ggtaaaccag caccgccagt tcctgggcag 34800 ggcccttacc atatccgata ttacactggt cacgggagag gacaccattc cccggcgcaa 34860 aaaggcgtgg ggaggcagcg tgatctgcgc cacgcccgag atagcaagaa atgatataga 34920 gcgcggcctg gtcccgctcg aacagttcgg cctggtcata ttcgacgagg cccacagggc 34980 ggtgggcgac tatgcctatt cttccatagc gcgggcggta ggggataact ccaggatggt 35040 gggcatgact gcgacgcttc ccagcgagag ggagaaggca gacgagataa tgggcaccct 35100 gctctccagg agcatagccc agaggacaga agacgacccg gacgtaaagc cctatgtaca 35160 ggagactgcc accgagtgga taaaggtgga tcttcccccc gagatgaagg agatacagag 35220 gctcctcaag ctggccctcg acgagaggta ttcctccctc aagaggtgcg ggtacgatct 35280 tggctcgaac aggtcgctct cggcgctgct ccggctgcgc atggtggtgc ttggcggcaa 35340 caggcgcgcg gccaagccgc tgttcactgc gatacgcata acgtacgcgc taaacatatt 35400 cgaggcgcac ggggtcacgc cctttctaaa gttctgcgag aggacctcca agaaaaaggg 35460 cgtcggcgtg gcggagctgt tcgaacagga ccggaacttt acaggggcca tcgcgcgcgc 35520 aaaggccgcg caggcggcag gcatggagca tcccaagata ccaaagctcg aggatgccgt 35580 ccgcggggcc cggggaaagg cgctggtctt tacgagctat cgtgattctg tcgacctcat 35640 acactcaaga ctcaaggcgg ccgggataaa ctcgggcatc ctgataggaa aggcgggaga 35700 aaagggccta aagcagagaa aacaggtgga gactgtggca aagttccgtg acggcgggta 35760 cgacgtgctg gtatcgacga gggtcggcga ggaggggctc gacatatcgg aggtcaacct 35820 ggtgatattc tatgacaatg tgccaagctc gatcaggtac gtgcagagga gggggagaac 35880 aggcagaaag gacgccggca ggctgatagt attgatggca aaggggacga tagacgaggc 35940 atactattgg attggtcggc gcaagatgag cgccgccaag ggcatgggtg agaggatgaa 36000 ccggtcgctg gcggcaggcg gggctgctgc caaggccgct ccaaagggac tcgaggggta 36060 cttttagccg aggcgcttta tcacgtgata gccaaactct gactttatgg gttccgagat 36120 ctcgcctatc tgcagccgga acgcggcctc ttcaaacggc tttaccatct ttcccctgcc 36180 aaagtagccg agactgccgt ccctctttgc actgcccccg tccatggaga gctcctttgc 36240 gagcctgcca aacttttccc cggccttgag gcgctctttt actgctagcg cctcgccctg 36300 tttttttacc agtatgtgcg agcactttat cttgtctgcc atgtgcgctg ctcctttgta 36360 ccctgctata acttgtcgtg ctgccggggc gcggtcatcc cgcagggcct gtattctgcc 36420 aggaactgtt aatccgcagg gactggtttc cccgtattat cctgtcatat acagggggga 36480 ttcggcggtc cacgtgtatt aacacctaaa gcagggataa acgtgtgaga acaagtgggc 36540 acccggaacg aatgttacgg catactggag gataaccaga taaaggaact agaacaaggc 36600 agcggcatcg acgtaccgtt gctggaccac gagggcagtc agttccattc caacagaata 36660 catcttacac tgggcaatat cgtatctccg cttgagcttg gtgacggcag tatgacaaac 36720 cccgggacgg acctgacacc atacgacgta aagtcaatag gcatggggcg caccataaag 36780 cgatatgcaa agtaccgttc tgaaggatcg caggccgtcc gcatggatgt catattcatg 36840 tcccgtgccg cctgggatga gatggataaa ggcaaggcat gaccggccgg ttttggggca 36900 tactgccggt atacagcggg aacaggcatt cagagacttt ggcggattcc gtgtgacccg 36960 ccccaagcta aacttttaat tgggatccgg cgagccggcg cgtgtcatcg tactttacca 37020 taaagaccgc caacctggcc ctgcccgacg tggtcaaaaa gtacaaccac gtcctggcat 37080 gcaagagcga ggtgatgagg gccgagaagc agatccagac gtccatctcc tcgtctagcg 37140 ggctcgacaa gtactcggag ctcaagcaac agttcaactc ccggataacc gagttctacc 37200 gctcgataga agagctggaa aagaccggtg cggtggtcaa gagcatagac gagggcctgc 37260 tggactttcc cgcaaagcgc tttggggacg acatctggct gtgctggaag acaggcgagc 37320 gcgagatcaa gttctggcat gaaaaggact ctggttttgg cggaagaaag cccatagagg 37380 taagtgacga gtcactagtg tagatgctct ccgcctggtt gcgcgtaata cgcgtccgct 37440 tcctgctcgc gtcggtgata gccgtctcgg cgggcctcgc cctctcctgg tggcacggcc 37500 acgaaataga cgcattctcc gccgcgctca ccatggccgg cgtggccgcg ctccacgcaa 37560 gcgtggacat gctcaacgat tattcggact acaagcgcgg catagatacc ataaccaaga 37620 ggaccccgat gagcggcgga acaggggtgc tgccagaagg cctgcttacc cccggccagg 37680 tgcaccgcgc cggcatcata tcgctggtcc tgggctctgc tgtcggcgcg tactttgtgg 37740 tcacaacggg gcccgtcata gccatgatac tcggctttgc cgtagtctcg atatactttt 37800 actcgacgag gattgtagac tcgggcctct ccgaggtctt tgtggccgtc aagggggcga 37860 tgatcgtcct tggcgcctac tacatacagg cgcccgagat aacgcctgcc gccgttctgg 37920 tgggggcggc cgtgggcgcc ctctcgtcgg cggtcctctt tgtggcgtcg tttccagacc 37980 acgatgcgga caagtcccgc ggcagaaaga cgcttgttat aatcctgggc aaggagaggg 38040 cctcgcggat cctctgggtg ttccccgcag tggcatactc gtccgttata acgggggtca 38100 tcctgcagtt cctgccggtg catgcactaa ccatgctgct tgcagccccc cttgcagtaa 38160 ttgcggcaaa aggccttgcc agggagtacg gcggggacgg gatcatacgg gtcatgcgcg 38220 gcacgctgcg gtttagcagg gttgcaggcg ccctgctggt gttgggcatt ctgttgggct 38280 gagtggagct aggttcgaga cgatgtaagc ttaaactagg tatgcaattg gatcacttag 38340 attctactaa cacgccatcg tcttttacat caacgaatat agttcgtata acctgcgagt 38400 tatcatcatc cctgacgttc tcgggagtta tttcttttga ttccccgtct tgtagaacaa 38460 cgctgtactt atccataatt aggtaaaact tctacatgta taaaaacgta ctatcttcac 38520 ggggccccgg caggcctttg ggatttttcg ctgcgtcaag tccgagtgtt ttcagggtgt 38580 gtgggtactc tgaaaaatcc cgggggcttt ccggcagggt acacaagaat agttaagtaa 38640 tgcagcattc aaagtaaaac atggacatga acaagctctg cgggatatgc atgaggagtc 38700 tggtgggtga gatcatgcct tatttccatt acaagtgcca taccgcctgt gtaaaatggc 38760 atgaatccaa tcccgggttg tgtgccctgt gcaaaaagga tgtcacgggg attaaacatt 38820 ttacaaagat agggggaatg gtataccatg aagactgttg tacaatattt gtggaaaaag 38880 tcctgggtca aaagaacaac cccccaaaat tcccgtatac gtacagtttt acatgtccgt 38940 cgtgctcaca cgaggacagc gtggatacgg ccgtcaagat ggacaccggg ccgcagagat 39000 tcatctgcaa cgggtgcaga aaaaaggtaa aagccacggg taaaaggata agataggcat 39060 cttgattggc cccgccgtat acaagatgaa atcatgcatt ctaaatacca gggcttgaga 39120 acgcaataaa accccggatg cctgccggaa gagtccggtc ataaaatccg gcccccgtac 39180 cggaggatct gtcggttgtg ggatggatat catccacttg ccattacatc acgccaattc 39240 gcgtgcagtc ctgcccgtgc ggcagggata caaagccatg atgcagaact ggaggcaccc 39300 atccggggaa actgcaggct gccccggaat ctaaaggtgc aaatggctgc agaacccccg 39360 gaccggcggg tcgcaccccg ggcgggatac caacgaacgg acccgcaccg tacacttcat 39420 acacataaat cccgcctgaa cggtcgtccg cgcatgatca gcgggcacgc cacggccgag 39480 ggtacacgca ggatagccga gatgtcgggc gcccatatcg acaactacaa gatggtcgac 39540 gggctgcacc tctccaacgt ggggatgggc acctaccttg gcgacgcgga tgacgccacc 39600 gacagggccg tcacggacgc agtcaagagg tccgtcaaaa caggcataaa cgtcatagat 39660 acggcgataa actaccgcct ccagagggcc gagcgctctg tcggcagggc cgtcacggag 39720 ctctcagaag aggggctcgt atcaagggac caaatattca tatcgacaaa ggcgggctat 39780 gtaacaaacg actccgaggt ctcgcttgac ttttgggagt atgtgaaaaa agagtacgtc 39840 gggggcggcg tgatccaggc aggcgacata tcctccggat accactgcat gaagcccgcc 39900 tatctagagg accagctgaa gaggagcctt gcaaacatgg gcctcgactg tatcgacctt 39960 gtctacgtgc acaaccccgt cgaggggcag atcaaggacc gccccatacc ggagatcctc 40020 gactgtatag gagaggcctt tgccatgtac gagaaggcaa gggaggatgg ccgcatcaga 40080 tactatgggc tcgccacgtg ggagtgcttt cgtgttgcag gggacaaccc gcagaatgtc 40140 cagctcgaag acgttgtaaa gaaggccaaa gacgcaggcg gggacaacca cggattcaag 40200 ttcatacagc tgcccttcaa ccagtacttt gaccaggctt acatgctaaa gaaccagacg 40260 gtggacggca gaaagctgtc catactggat gcggcagtat cccttggcgt cggtgtgttc 40320 acgagtgtcc cgttcatgca aggcaagctg ctcgagcctg gcctgctgcc ggagtttggc 40380 gggctctccc ccgccctgcg atccctgcag tttatcaggt ctacaccagg cgtgcttgcc 40440 cccctgccgg ggcacaactc agctgcgcat acagacgaga acctcaagat catgggcgtg 40500 ccccccatcc cgcctgacaa gttcggggag cttgtggcca gcctcacctc gtggtcgccc 40560 ggtcagaaat agccggtcag ctgcctctcg ggcattatct ggtcgagcac cttttttgag 40620 agccgtgaat cggcggaatc ctgcacgttg cgccgggccc ttgccacgtt ggcaggatac 40680 aggtctatcc cggtaaagcc cctcttgagg catgcagaga ctattcccgt ggtccccctg 40740 cctgcaaacg ggtccagcac gtaatcgccc tcttttgtgg caaactttac tatcctggat 40800 acaaggtctt ctgggaatac cgcaaagtgc tcgtttccat ggtgcgcctt tgtggatatc 40860 tcccagacgt tccccgggtt cttgccccgc gggttgcatg cggcaaatat cggatagtgc 40920 tcgtggcccc ctatcctctt gcgcgtcgca tgcctccgga acttgcgata gcacgtgggg 40980 cagtactttt cggggtcata gccgtgggcc catgatattt ccccggtggt tggcagctcg 41040 tcaaacggcg taccaggcgt tgagccgtgt atcacggctg caatcctccc tattgcctcg 41100 ggatccctct tcccgggggc gaactgcagc cggtcatttg cgggtttgct gtttatcccg 41160 ctcagggcct cgttgccctg gacgcgtatc gggtttatgt cataggcggg ggtatccgac 41220 tttgagagga ccagaacaaa ctcgtacgcc tgcgtcaggt tttgccgcga gctttgcgag 41280 atggcgtttc gcttgtacca gattatatcc tcctggaaat ggtacccaag atccaccagc 41340 cttagcgcga gccggtgcgg gaccatcagc ttgtggcgcc gcctcctggt atcacctatc 41400 actatgaaga ggctcccgtc gtctgttagc aggtccatgc agctcttgaa tactcctgcc 41460 agctcctcga tgaactcgtc tggcgtcttt tcctggccca gctcggaggg ctccgacccg 41520 tactttctgt gcccgtaata gggaggggat gttaccgcca gtctgtacct gccgcgctcg 41580 gctgtattct ttgccagccg cggcagcacc tcccgggcgt cgccctgcag tatctggaac 41640 ttttcactca agataggccc ccgtgccatc catctgcccc tgcgcgatcc gacaagtcgt 41700 attcatcttg taccgcggca cccgcgccgt cttaaaatct ttgtagctta taccggcgcg 41760 ccgcagatgc ggtacaatcc ctccggtgct cccgcgatcc ggcgcggtgc catcagccgc 41820 cccgtttccc cttccggggg ccccgccacc atacacgtgg tataaacaga ggccggacgg 41880 cgcggaccac atgtggataa aggacgagtt tcttggcaaa ggcaacaaga tgaggctgct 41940 ctacatcata ctgcccatct acgggtacct cttcctggag tactggccgt tcctgccctg 42000 gatggctaca ttctggtggt cggtggcgct cagcccccct attctcctga tgccttatgc 42060 cggggaggcc ataggtcagc tgatcggcgg gcatgtattg tttggagttg tcacaaagta 42120 tgtctatgcg gcagtatggc tgggcatggc acacgggata atcctcctga cagggcgcct 42180 cagggccagg gctggtaccc tgcgcgaatc ccccgcatag ccccggcagg gcccgttgtt 42240 ccggatggcc aaggccggcg catacatccc atgatgcata gaccgggggg acatgatcgc 42300 agcagatcgt tccatgccgc ccccgtacgc tctggggcgc acctagtcag ggcggggccc 42360 cccgcggtcc aattaaatac ggcaaggaac ggggggtctc gttgaaactg cagggcagga 42420 ctgccgtgat cc 42432 <210> SEQ ID NO 3 <211> LENGTH: 10419 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(10419) <400> SEQUENCE: 3 atc ccc gcg ccg cca gga gag ggc agc ctt ggc ggg gtg gca ata tcc 48 Met Pro Ala Pro Pro Gly Glu Gly Ser Leu Gly Gly Val Ala Ile Ser 1 5 10 15 gac gac ggg agg tac atg tac gca atc ggc agg gat ctg ctc aca gta 96 Asp Asp Gly Arg Tyr Met Tyr Ala Ile Gly Arg Asp Leu Leu Thr Val 20 25 30 tac cgg tat aca atg aac ccg ccc cat gac ata gcc tcg gcc gcg ctc 144 Tyr Arg Tyr Thr Met Asn Pro Pro His Asp Ile Ala Ser Ala Ala Leu 35 40 45 ggt gcg cag tca ttt tct ctg cct ggc ggc atc agc ccc gcc ccc ggc 192 Gly Ala Gln Ser Phe Ser Leu Pro Gly Gly Ile Ser Pro Ala Pro Gly 50 55 60 gcg ccg acc ggc ctt gac atc tcg gat gac ggc cgc cac ctg tac gtc 240 Ala Pro Thr Gly Leu Asp Ile Ser Asp Asp Gly Arg His Leu Tyr Val 65 70 75 80 ccg gac gaa aac ggc gtc gtg tac agg ttt gat ctg gaa agc ccg tac 288 Pro Asp Glu Asn Gly Val Val Tyr Arg Phe Asp Leu Glu Ser Pro Tyr 85 90 95 agg cta gac ggc ggc acg ttt ggc tct tct gtt tat gtg gga tcc gac 336 Arg Leu Asp Gly Gly Thr Phe Gly Ser Ser Val Tyr Val Gly Ser Asp 100 105 110 gtt gcc gcg ccc cgc ggc gta tac gtg gcg ccg ggc ggc agc ctc atg 384 Val Ala Ala Pro Arg Gly Val Tyr Val Ala Pro Gly Gly Ser Leu Met 115 120 125 ctg gtc tcg gat agt gca gac ggc acc atc cac agg tac gag ctg gca 432 Leu Val Ser Asp Ser Ala Asp Gly Thr Ile His Arg Tyr Glu Leu Ala 130 135 140 agc ccg tac gag ccg gcg ggc gcg gca aac agg gga tca ttc gac gtg 480 Ser Pro Tyr Glu Pro Ala Gly Ala Ala Asn Arg Gly Ser Phe Asp Val 145 150 155 160 tcg gat atg gac ggc tcg cct gtc ggg gcg ggg ttt gcg ggc ggc ctg 528 Ser Asp Met Asp Gly Ser Pro Val Gly Ala Gly Phe Ala Gly Gly Leu 165 170 175 cac atg tat gtc gcg gga aac gac acc gga agg gtc tac cag tat ccg 576 His Met Tyr Val Ala Gly Asn Asp Thr Gly Arg Val Tyr Gln Tyr Pro 180 185 190 gcg ggc acg cac cag ata cag gag gca gcc gca ggg ccg cgg ctg ctc 624 Ala Gly Thr His Gln Ile Gln Glu Ala Ala Ala Gly Pro Arg Leu Leu 195 200 205 tcg gcc gtc ctg gac aaa gac gga acc ctg agg gcg gcc ttt gac ggc 672 Ser Ala Val Leu Asp Lys Asp Gly Thr Leu Arg Ala Ala Phe Asp Gly 210 215 220 acg gta gac gcg gga tcc gtg cag ccc ggg atg atc acc atc agg gac 720 Thr Val Asp Ala Gly Ser Val Gln Pro Gly Met Ile Thr Ile Arg Asp 225 230 235 240 ggc cat ggc tcc aac acg gga ata ccc ctt ttg ctt gcc ggg ggt gcc 768 Gly His Gly Ser Asn Thr Gly Ile Pro Leu Leu Leu Ala Gly Gly Ala 245 250 255 gcg gac tct gat gtc atg aca ttt gtg gtc ccc gag aaa gac agg gca 816 Ala Asp Ser Asp Val Met Thr Phe Val Val Pro Glu Lys Asp Arg Ala 260 265 270 gag gct gcc gca tac ggg gac cag tcg ctg cat gtt ccc gcc gcg gcg 864 Glu Ala Ala Ala Tyr Gly Asp Gln Ser Leu His Val Pro Ala Ala Ala 275 280 285 ctg gcg ggg act ggc ggc ggg ccg ttt gtg ccc gac ttt tcc ggg ggc 912 Leu Ala Gly Thr Gly Gly Gly Pro Phe Val Pro Asp Phe Ser Gly Gly 290 295 300 tcg ctg ctg gcg tcc ctg tac cgg cac gag cgg ccg ttc cag ggc gag 960 Ser Leu Leu Ala Ser Leu Tyr Arg His Glu Arg Pro Phe Gln Gly Glu 305 310 315 320 gag atg gca cgg acg gag aga tcc gac agg tac gcg ctt act gta act 1008 Glu Met Ala Arg Thr Glu Arg Ser Asp Arg Tyr Ala Leu Thr Val Thr 325 330 335 gca ggc ggg agt cag atg cat gtg ggc ggc gcc ggc gga aac atc acc 1056 Ala Gly Gly Ser Gln Met His Val Gly Gly Ala Gly Gly Asn Ile Thr 340 345 350 tgg tac gat ctt ggc acg ccc cat gac ata acg acc ggc gtc cgc gcg 1104 Trp Tyr Asp Leu Gly Thr Pro His Asp Ile Thr Thr Gly Val Arg Ala 355 360 365 gga tcc gac atc ctg ccg gcg tat cca tcc gcg ggc aga aac gtg gtg 1152 Gly Ser Asp Ile Leu Pro Ala Tyr Pro Ser Ala Gly Arg Asn Val Val 370 375 380 ccg tca ata acg ggc att gcc ttc tcg gat gac ggc atg cgg ttg ttt 1200 Pro Ser Ile Thr Gly Ile Ala Phe Ser Asp Asp Gly Met Arg Leu Phe 385 390 395 400 gca gca aac cgg ggc gac cgc att cca atg tac cag ctg gac agc ccg 1248 Ala Ala Asn Arg Gly Asp Arg Ile Pro Met Tyr Gln Leu Asp Ser Pro 405 410 415 tac gac ata ggg agc gcc agc ctc gag gga acc ctg ttt acg ggg ttc 1296 Tyr Asp Ile Gly Ser Ala Ser Leu Glu Gly Thr Leu Phe Thr Gly Phe 420 425 430 cag tcg ggc att gca ttc tcg gat gac ggc acg cgc atg ttt gcc gcc 1344 Gln Ser Gly Ile Ala Phe Ser Asp Asp Gly Thr Arg Met Phe Ala Ala 435 440 445 ctg ctc acc gag aat gcc ata cgg cag tac gac ctg gag ggc ccc tat 1392 Leu Leu Thr Glu Asn Ala Ile Arg Gln Tyr Asp Leu Glu Gly Pro Tyr 450 455 460 gac ata cgc ggg gcg ggc aat gcg ggc cag tac gac ctg gac atc ccg 1440 Asp Ile Arg Gly Ala Gly Asn Ala Gly Gln Tyr Asp Leu Asp Ile Pro 465 470 475 480 ctg cac cca gga ctg ctg ttc ctg ctg acc tcg ggg gtg cac ttt tcg 1488 Leu His Pro Gly Leu Leu Phe Leu Leu Thr Ser Gly Val His Phe Ser 485 490 495 ccc gac ggg acg agg atg ttc gtc ggc gag ggg ata tca gat gcg gag 1536 Pro Asp Gly Thr Arg Met Phe Val Gly Glu Gly Ile Ser Asp Ala Glu 500 505 510 gat gcc aac gcg aac agg gat gtc aac gtc aac ctg tgg cac agg ttt 1584 Asp Ala Asn Ala Asn Arg Asp Val Asn Val Asn Leu Trp His Arg Phe 515 520 525 gat ctc tcc acg ccg ttt gat gtg ctc acg gcg gag cgc gtg gac acg 1632 Asp Leu Ser Thr Pro Phe Asp Val Leu Thr Ala Glu Arg Val Asp Thr 530 535 540 tac gag tac agc acg ggg ccg gca ggc gat ctc gag gac ctc tcc ctg 1680 Tyr Glu Tyr Ser Thr Gly Pro Ala Gly Asp Leu Glu Asp Leu Ser Leu 545 550 555 560 tcc cct gac ggc cgc aga ttg tac acc ctg tcg agc gag agg gta agc 1728 Ser Pro Asp Gly Arg Arg Leu Tyr Thr Leu Ser Ser Glu Arg Val Ser 565 570 575 tca agc gag tat aca atc acc cgg gcc cag tac tgg ctg cca gaa ccg 1776 Ser Ser Glu Tyr Thr Ile Thr Arg Ala Gln Tyr Trp Leu Pro Glu Pro 580 585 590 tac gac gtg acg ccg ccg tac cat gtg ccg tca ttc aac gca agc cag 1824 Tyr Asp Val Thr Pro Pro Tyr His Val Pro Ser Phe Asn Ala Ser Gln 595 600 605 ggg ggc aac ctg gca gac ccc tac ggg atg gcc ttc tcg ccc gac ggg 1872 Gly Gly Asn Leu Ala Asp Pro Tyr Gly Met Ala Phe Ser Pro Asp Gly 610 615 620 acc agg ctg ctg gtc acg ggg cac ggg cag acg aat gca aag ctg ttc 1920 Thr Arg Leu Leu Val Thr Gly His Gly Gln Thr Asn Ala Lys Leu Phe 625 630 635 640 cac ctg aat ccg ccc ttt gat gtg ggc acg gcc gtg ttc cac gac cac 1968 His Leu Asn Pro Pro Phe Asp Val Gly Thr Ala Val Phe His Asp His 645 650 655 ggc agg ttc cgc ccc ggg ggg ccc gca agc gag atc gag gcg tcg ggg 2016 Gly Arg Phe Arg Pro Gly Gly Pro Ala Ser Glu Ile Glu Ala Ser Gly 660 665 670 ata tcc ctg tct gcc gac ggc tcc agg atg ttt ctc tcc gac cgc ggc 2064 Ile Ser Leu Ser Ala Asp Gly Ser Arg Met Phe Leu Ser Asp Arg Gly 675 680 685 cgc ggg gcc atc agc cag tac acg ctg gtt gcg ccc ttt gat gtg gag 2112 Arg Gly Ala Ile Ser Gln Tyr Thr Leu Val Ala Pro Phe Asp Val Glu 690 695 700 ttt gcg tcg gat gtg tcc gcg gat ggg cag ctc gac gtt ggc gcc cag 2160 Phe Ala Ser Asp Val Ser Ala Asp Gly Gln Leu Asp Val Gly Ala Gln 705 710 715 720 gat gcg ctt ccc ggc ggg ctt gcc ttc tcg ccc ggg ggg acg agg cta 2208 Asp Ala Leu Pro Gly Gly Leu Ala Phe Ser Pro Gly Gly Thr Arg Leu 725 730 735 ttc atg gtg gga ggc atg gac agg tca gtt cac atg tat tcc ctg aat 2256 Phe Met Val Gly Gly Met Asp Arg Ser Val His Met Tyr Ser Leu Asn 740 745 750 acg ccg ttt gac ctg ggc ggg gca gag cat gcg gcg tcg ttt ggc gtg 2304 Thr Pro Phe Asp Leu Gly Gly Ala Glu His Ala Ala Ser Phe Gly Val 755 760 765 ggg gac agg gtc tcg gat ccc ctc ggc atc gcc ttt ggg aac ggg ggg 2352 Gly Asp Arg Val Ser Asp Pro Leu Gly Ile Ala Phe Gly Asn Gly Gly 770 775 780 act aaa atg cta ata gcc gat acg aca ggc ttt gtg cac ggg tac gac 2400 Thr Lys Met Leu Ile Ala Asp Thr Thr Gly Phe Val His Gly Tyr Asp 785 790 795 800 ctt ggc gcc ccg tac gat atc tcg ggc ccc gcg tac agc ggc ata ttt 2448 Leu Gly Ala Pro Tyr Asp Ile Ser Gly Pro Ala Tyr Ser Gly Ile Phe 805 810 815 gac gcc ggc ggc agc atc cgg gac gtg gcc gtc ggc ggg ggg tcc atg 2496 Asp Ala Gly Gly Ser Ile Arg Asp Val Ala Val Gly Gly Gly Ser Met 820 825 830 ttc ata ctc gag ggg gag acg gac cgg gtg tat gag cac cgc ccc ggc 2544 Phe Ile Leu Glu Gly Glu Thr Asp Arg Val Tyr Glu His Arg Pro Gly 835 840 845 ata tac ccg gtg gtc tca gca ctg gac ggg ccg gcg ctg gtc tct gct 2592 Ile Tyr Pro Val Val Ser Ala Leu Asp Gly Pro Ala Leu Val Ser Ala 850 855 860 gca gca gat gca agg gtg ggt gcg gcc gag gtg ctc ttt gat cgc gcg 2640 Ala Ala Asp Ala Arg Val Gly Ala Ala Glu Val Leu Phe Asp Arg Ala 865 870 875 880 gtg gat gtt ggc ggg ata gac ccc ggg ggg gtc cgc ata gtg gat gca 2688 Val Asp Val Gly Gly Ile Asp Pro Gly Gly Val Arg Ile Val Asp Ala 885 890 895 gca ggc ccc ctg ccc ggc gtg gtg atc tcg gat gcc gtc ata cca ggc 2736 Ala Gly Pro Leu Pro Gly Val Val Ile Ser Asp Ala Val Ile Pro Gly 900 905 910 gag gat ccc ggc gtg gcc agg ttc agc ctg tcg gac gcg gag gtc ctt 2784 Glu Asp Pro Gly Val Ala Arg Phe Ser Leu Ser Asp Ala Glu Val Leu 915 920 925 gcc gtg tcc ggg tat gcc gag ccg agt ctg gtc ttt gga agg cat gcg 2832 Ala Val Ser Gly Tyr Ala Glu Pro Ser Leu Val Phe Gly Arg His Ala 930 935 940 gtg ccg ggc gcg gca ggc ggc aca ttt ccc tcc cag ata ggc aac gcc 2880 Val Pro Gly Ala Ala Gly Gly Thr Phe Pro Ser Gln Ile Gly Asn Ala 945 950 955 960 acg gag ctt gtg gga tcg att ccg aat ccg acc ctg gat ttt ggg acg 2928 Thr Glu Leu Val Gly Ser Ile Pro Asn Pro Thr Leu Asp Phe Gly Thr 965 970 975 acc ctg acg ggg gcg gca ttc tcg gcg gac ggg acg gtg gta ttt ctc 2976 Thr Leu Thr Gly Ala Ala Phe Ser Ala Asp Gly Thr Val Val Phe Leu 980 985 990 tca gac ggc ccc acc ggc agg gtg tac ccg tat tca ctg aat atc ccc 3024 Ser Asp Gly Pro Thr Gly Arg Val Tyr Pro Tyr Ser Leu Asn Ile Pro 995 1000 1005 ttt gac ata tcg tct gcg gcg cct ggg ggc ttt gta atc gtg ccc gtc 3072 Phe Asp Ile Ser Ser Ala Ala Pro Gly Gly Phe Val Ile Val Pro Val 1010 1015 1020 gga gtc tcg gac att gcg ttt tct gcc gac ggg cgg aac atg cta gtc 3120 Gly Val Ser Asp Ile Ala Phe Ser Ala Asp Gly Arg Asn Met Leu Val 1025 1030 1035 1040 gcg gac gaa acc ggg gga ata cac agg tac ctg gcc cgc agc ccg tac 3168 Ala Asp Glu Thr Gly Gly Ile His Arg Tyr Leu Ala Arg Ser Pro Tyr 1045 1050 1055 gag ata ggc acg gat ttc atc aaa tca tcc ctg ggt gag ttt gtc gag 3216 Glu Ile Gly Thr Asp Phe Ile Lys Ser Ser Leu Gly Glu Phe Val Glu 1060 1065 1070 aca ttc tcg gcg gcg ccc cgc gtg cag gat ctt gcc ggc atc gcc ttt 3264 Thr Phe Ser Ala Ala Pro Arg Val Gln Asp Leu Ala Gly Ile Ala Phe 1075 1080 1085 tcg cac gac ggc atg atc atg ctt gcg gcc ggc ggc tcg ggg tct gtg 3312 Ser His Asp Gly Met Ile Met Leu Ala Ala Gly Gly Ser Gly Ser Val 1090 1095 1100 cac cgg tac tcg ctg cca tcc ccg tat gca gta tcg ggg gcc aaa tac 3360 His Arg Tyr Ser Leu Pro Ser Pro Tyr Ala Val Ser Gly Ala Lys Tyr 1105 1110 1115 1120 gag gag acg gcg atg att ggc ggg agc ccg tcg ggg ctg gag ttc tcg 3408 Glu Glu Thr Ala Met Ile Gly Gly Ser Pro Ser Gly Leu Glu Phe Ser 1125 1130 1135 tcc gac ggc ctg agg atg ttt gtt ccc gat gcg ggc tcg gag acg gcg 3456 Ser Asp Gly Leu Arg Met Phe Val Pro Asp Ala Gly Ser Glu Thr Ala 1140 1145 1150 gca gtc tac ggc ctt gcc gcc ccc tac ggg att ggc gag gcg gag ccg 3504 Ala Val Tyr Gly Leu Ala Ala Pro Tyr Gly Ile Gly Glu Ala Glu Pro 1155 1160 1165 ctg ccg ccg ctg ttc ctg ggg gta ggg gca gaa gag gcc acg ctc tcg 3552 Leu Pro Pro Leu Phe Leu Gly Val Gly Ala Glu Glu Ala Thr Leu Ser 1170 1175 1180 cct gac ggc agg cac atc cta gtt ccc ggc agg ccc ggc ctg tcc cag 3600 Pro Asp Gly Arg His Ile Leu Val Pro Gly Arg Pro Gly Leu Ser Gln 1185 1190 1195 1200 tac tcg ctg ttc tcg acg aat ctt gag ctg tgc gcg gag ccc cgg ggc 3648 Tyr Ser Leu Phe Ser Thr Asn Leu Glu Leu Cys Ala Glu Pro Arg Gly 1205 1210 1215 att gac ggg gga tcg tgc gaa gat ggg ata tac gcc ttt gag agt ccg 3696 Ile Asp Gly Gly Ser Cys Glu Asp Gly Ile Tyr Ala Phe Glu Ser Pro 1220 1225 1230 ggc agg ggc gag ggc gta tcg ctt gcc gcc tcg ata acg gcg gca gac 3744 Gly Arg Gly Glu Gly Val Ser Leu Ala Ala Ser Ile Thr Ala Ala Asp 1235 1240 1245 ggg cca gga att ggc gag ctg cac ggg ttt gca ggc ccg ccg atg ccg 3792 Gly Pro Gly Ile Gly Glu Leu His Gly Phe Ala Gly Pro Pro Met Pro 1250 1255 1260 gcg cct gtc atg gag cag gtc aca ctg gat tcg cgg gag ggc aca ctc 3840 Ala Pro Val Met Glu Gln Val Thr Leu Asp Ser Arg Glu Gly Thr Leu 1265 1270 1275 1280 agg gtc agg ctg gac agg aca gtg gac gtc gac acg gtg cgc ccc tat 3888 Arg Val Arg Leu Asp Arg Thr Val Asp Val Asp Thr Val Arg Pro Tyr 1285 1290 1295 aag atg tgg gtg gag gat tca gac ggc agc cag aca acc ctg gca aat 3936 Lys Met Trp Val Glu Asp Ser Asp Gly Ser Gln Thr Thr Leu Ala Asn 1300 1305 1310 tca aca ctg ttg aat gcc gaa aac tcg aac att ctg ctc ttc agg ctg 3984 Ser Thr Leu Leu Asn Ala Glu Asn Ser Asn Ile Leu Leu Phe Arg Leu 1315 1320 1325 gat gat gcg gcc gca ggc aaa ata tcc ggg tat aca tcc ccc gtg ttt 4032 Asp Asp Ala Ala Ala Gly Lys Ile Ser Gly Tyr Thr Ser Pro Val Phe 1330 1335 1340 cgc acg tgg tcg tcg ccg ttc ctg ggc aca gac gga gcc acc agg ccc 4080 Arg Thr Trp Ser Ser Pro Phe Leu Gly Thr Asp Gly Ala Thr Arg Pro 1345 1350 1355 1360 cat acg ctg ggc ttt gga gac gtg cgc ctt gcg gat ata tac gat gca 4128 His Thr Leu Gly Phe Gly Asp Val Arg Leu Ala Asp Ile Tyr Asp Ala 1365 1370 1375 tcc ggg gat gtc ccg tcg ccg tcg ggc att gag ttt tca gat gac ggc 4176 Ser Gly Asp Val Pro Ser Pro Ser Gly Ile Glu Phe Ser Asp Asp Gly 1380 1385 1390 atg agg atg ttc gtt acg ggg atc ggc acg cca ggc atc aac ata ttc 4224 Met Arg Met Phe Val Thr Gly Ile Gly Thr Pro Gly Ile Asn Ile Phe 1395 1400 1405 aca ctg tcc gcc ccc ttt gac ata aca ttg ccg aag cat tcc ggc tca 4272 Thr Leu Ser Ala Pro Phe Asp Ile Thr Leu Pro Lys His Ser Gly Ser 1410 1415 1420 acc aac ata ggc ggc ctg tcc gtg tct gat ctg gca ttt gca aac aat 4320 Thr Asn Ile Gly Gly Leu Ser Val Ser Asp Leu Ala Phe Ala Asn Asn 1425 1430 1435 1440 ggg aac agc ctc acg gtg ctc gat gtg gac ggg gtg ttg cgc gtc tac 4368 Gly Asn Ser Leu Thr Val Leu Asp Val Asp Gly Val Leu Arg Val Tyr 1445 1450 1455 gcc ctt ggg gac gat tac aat gtg gtc acc gga acc acc cag aag ttt 4416 Ala Leu Gly Asp Asp Tyr Asn Val Val Thr Gly Thr Thr Gln Lys Phe 1460 1465 1470 agg att acg ctc gat acc aca cag ggc ata ccc aat tcc att tac aca 4464 Arg Ile Thr Leu Asp Thr Thr Gln Gly Ile Pro Asn Ser Ile Tyr Thr 1475 1480 1485 tct ccg gac ggc ctg tca cag ttt gtg gca tat gat gac agg att gac 4512 Ser Pro Asp Gly Leu Ser Gln Phe Val Ala Tyr Asp Asp Arg Ile Asp 1490 1495 1500 ttg tac gtg ctt ggc agc cca aac gac ata tcg tcg aca acc gag ata 4560 Leu Tyr Val Leu Gly Ser Pro Asn Asp Ile Ser Ser Thr Thr Glu Ile 1505 1510 1515 1520 atc ccg tat tcg ctg cca agg ccg gac ccg cca acc ggc atg gac ttt 4608 Ile Pro Tyr Ser Leu Pro Arg Pro Asp Pro Pro Thr Gly Met Asp Phe 1525 1530 1535 acg cca gac ggg cgc agg atg ttc ctg tcc acc gag aac ggg ata gac 4656 Thr Pro Asp Gly Arg Arg Met Phe Leu Ser Thr Glu Asn Gly Ile Asp 1540 1545 1550 cag tac ctg ctt tca gaa ccg ttt gca gtc acc acg tcg gta ttt ttg 4704 Gln Tyr Leu Leu Ser Glu Pro Phe Ala Val Thr Thr Ser Val Phe Leu 1555 1560 1565 cgc acg atc ccc att gac gga ggg gcg gag gga ata cgg ttt gta gac 4752 Arg Thr Ile Pro Ile Asp Gly Gly Ala Glu Gly Ile Arg Phe Val Asp 1570 1575 1580 aac gga agg ggc ctg ttt gtg ccg ggc gcc gac ggc atc atc cag agg 4800 Asn Gly Arg Gly Leu Phe Val Pro Gly Ala Asp Gly Ile Ile Gln Arg 1585 1590 1595 1600 cac gag ctc atc tac ccg tac ggg gcc agc acg tcg ttg ttg gag acc 4848 His Glu Leu Ile Tyr Pro Tyr Gly Ala Ser Thr Ser Leu Leu Glu Thr 1605 1610 1615 gtc agg gac ggc gtg acg gac ggc ggt ccg ggc gag aac ccg gcc gcc 4896 Val Arg Asp Gly Val Thr Asp Gly Gly Pro Gly Glu Asn Pro Ala Ala 1620 1625 1630 gga gag atc cgc ctt gcg ggc aca ttc aat gca tcc gat aat gta cag 4944 Gly Glu Ile Arg Leu Ala Gly Thr Phe Asn Ala Ser Asp Asn Val Gln 1635 1640 1645 tcg ccg tcg ggc att gag ttt tca ggc gac ggc acg ggg atg ttt gtt 4992 Ser Pro Ser Gly Ile Glu Phe Ser Gly Asp Gly Thr Gly Met Phe Val 1650 1655 1660 acc ggg ttt ggg gcc gcg ggc gtg aat gaa ttc tcc ctg tcc gcc ccc 5040 Thr Gly Phe Gly Ala Ala Gly Val Asn Glu Phe Ser Leu Ser Ala Pro 1665 1670 1675 1680 ttt gat aca acc ctc ccg gtg cat gtg gaa ttg cac gat ata ggc ggc 5088 Phe Asp Thr Thr Leu Pro Val His Val Glu Leu His Asp Ile Gly Gly 1685 1690 1695 cag ccg gca gtt gat ctg gcg ttt gca gaa gat ggc agg acc ctc ctg 5136 Gln Pro Ala Val Asp Leu Ala Phe Ala Glu Asp Gly Arg Thr Leu Leu 1700 1705 1710 ttg ctg gcc gcg gat gga aca ctg gat ttc tac agc ctt gcc ggt gat 5184 Leu Leu Ala Ala Asp Gly Thr Leu Asp Phe Tyr Ser Leu Ala Gly Asp 1715 1720 1725 gcc tat gat ata ggg gaa gca tcc cgt act ttt caa gtg ccg ttt gag 5232 Ala Tyr Asp Ile Gly Glu Ala Ser Arg Thr Phe Gln Val Pro Phe Glu 1730 1735 1740 gat gcc gcg ggt gct gtg ccc ggc gcc ttt tac cag cct ccg gat ggc 5280 Asp Ala Ala Gly Ala Val Pro Gly Ala Phe Tyr Gln Pro Pro Asp Gly 1745 1750 1755 1760 tcg tct att att gcc gca ttt gac ggc agg att gac cag tat gtg gtg 5328 Ser Ser Ile Ile Ala Ala Phe Asp Gly Arg Ile Asp Gln Tyr Val Val 1765 1770 1775 atc ccc ttc gag ttc gtg tca tat cca ctg aca agg ccc ggc acg ccc 5376 Ile Pro Phe Glu Phe Val Ser Tyr Pro Leu Thr Arg Pro Gly Thr Pro 1780 1785 1790 aca ggg att gac ttt gcg cca gac ggg cgc tgg atg ttc ctg tcc acc 5424 Thr Gly Ile Asp Phe Ala Pro Asp Gly Arg Trp Met Phe Leu Ser Thr 1795 1800 1805 gag aac ggg ata gac cag tac ctg ctg tcg atc ccc ttt gac gtg cgc 5472 Glu Asn Gly Ile Asp Gln Tyr Leu Leu Ser Ile Pro Phe Asp Val Arg 1810 1815 1820 agc ctg acg tat acg gga acc att cca gta gac ggg gtg gag gga atg 5520 Ser Leu Thr Tyr Thr Gly Thr Ile Pro Val Asp Gly Val Glu Gly Met 1825 1830 1835 1840 cag ttt gcg gac aac ggc agg gca ctg ttt ttg gcg gac agt gaa ggc 5568 Gln Phe Ala Asp Asn Gly Arg Ala Leu Phe Leu Ala Asp Ser Glu Gly 1845 1850 1855 ttg att tac aat tat gac ctg gag gac ccg tat gct ctg gat ggc aac 5616 Leu Ile Tyr Asn Tyr Asp Leu Glu Asp Pro Tyr Ala Leu Asp Gly Asn 1860 1865 1870 aca att tcc gtg gaa ttc tcg ttt gac ggt agc gtg atg tat gtg ctg 5664 Thr Ile Ser Val Glu Phe Ser Phe Asp Gly Ser Val Met Tyr Val Leu 1875 1880 1885 gag tac gac aca aaa agg gtg gtc tcg tac gag ttg gag ttt ccc ttt 5712 Glu Tyr Asp Thr Lys Arg Val Val Ser Tyr Glu Leu Glu Phe Pro Phe 1890 1895 1900 gac gta tcg agc aga aca cgt gca gac acg ctg gac ata cca caa att 5760 Asp Val Ser Ser Arg Thr Arg Ala Asp Thr Leu Asp Ile Pro Gln Ile 1905 1910 1915 1920 gac tca cca aga cac gtt gca gtc tcg atg ccc ggc aac cac ctg tac 5808 Asp Ser Pro Arg His Val Ala Val Ser Met Pro Gly Asn His Leu Tyr 1925 1930 1935 ata aca aac tcg gtg ttt ggg gaa gat gac acc ata cac tcc tat gga 5856 Ile Thr Asn Ser Val Phe Gly Glu Asp Asp Thr Ile His Ser Tyr Gly 1940 1945 1950 ata tct aac aat gac ata tcg tcg gca tca tac atc ggc gag gaa ggc 5904 Ile Ser Asn Asn Asp Ile Ser Ser Ala Ser Tyr Ile Gly Glu Glu Gly 1955 1960 1965 atc ccg gaa ccc gtg ata aac ggg att gac ttt tcc aac aac ggc cgc 5952 Ile Pro Glu Pro Val Ile Asn Gly Ile Asp Phe Ser Asn Asn Gly Arg 1970 1975 1980 cgc atg ttt ctg att ggg ggc aac ggg ttc gac tac cag gtg ata cat 6000 Arg Met Phe Leu Ile Gly Gly Asn Gly Phe Asp Tyr Gln Val Ile His 1985 1990 1995 2000 gac tac atg cta ggc aca aga tac gac ata tcc agc agg agc ctg ctt 6048 Asp Tyr Met Leu Gly Thr Arg Tyr Asp Ile Ser Ser Arg Ser Leu Leu 2005 2010 2015 gat aca tat gcc att cca ggg ccg gtt gtt ttt ccc gcg ggc ctt gat 6096 Asp Thr Tyr Ala Ile Pro Gly Pro Val Val Phe Pro Ala Gly Leu Asp 2020 2025 2030 ttc tcg ttt gac agg ctg tcc atg ttt ata ata agc acc gcc ggt tcg 6144 Phe Ser Phe Asp Arg Leu Ser Met Phe Ile Ile Ser Thr Ala Gly Ser 2035 2040 2045 gta tac agg tac ggc ctg gac gat ccg ttc ata gtt gaa aca atg gac 6192 Val Tyr Arg Tyr Gly Leu Asp Asp Pro Phe Ile Val Glu Thr Met Asp 2050 2055 2060 tat cag gag tct ttc cgg ctg ccc gta cca tca gcg gct gat aat tca 6240 Tyr Gln Glu Ser Phe Arg Leu Pro Val Pro Ser Ala Ala Asp Asn Ser 2065 2070 2075 2080 ata tcg gat ctg gca ttc ggc agc agc ggc ctg aat gcc gta ata tcg 6288 Ile Ser Asp Leu Ala Phe Gly Ser Ser Gly Leu Asn Ala Val Ile Ser 2085 2090 2095 cac gag ggg ctc gac acc ctg tac agc ttt gta ctg gac atc ccg tat 6336 His Glu Gly Leu Asp Thr Leu Tyr Ser Phe Val Leu Asp Ile Pro Tyr 2100 2105 2110 ggg gcc gaa ttg gat att gac agg ctt gag ctt ccg ctg gtg ggg gtt 6384 Gly Ala Glu Leu Asp Ile Asp Arg Leu Glu Leu Pro Leu Val Gly Val 2115 2120 2125 ccg acg gga ttc gag ttc tcg gac aac ggg cgc cag ttg tac att ggc 6432 Pro Thr Gly Phe Glu Phe Ser Asp Asn Gly Arg Gln Leu Tyr Ile Gly 2130 2135 2140 gcg ttt cgt gac tct caa tcc tcg cca ggc acc ctg cct gcg ggc ctg 6480 Ala Phe Arg Asp Ser Gln Ser Ser Pro Gly Thr Leu Pro Ala Gly Leu 2145 2150 2155 2160 cag cgc tat gag ctt ggc ata cca tat gac ctg gct tcg gct gta ttt 6528 Gln Arg Tyr Glu Leu Gly Ile Pro Tyr Asp Leu Ala Ser Ala Val Phe 2165 2170 2175 gcg cag tcc ctg gga ata ttc gat ttt cct ccc ttc aac ggc atg cgg 6576 Ala Gln Ser Leu Gly Ile Phe Asp Phe Pro Pro Phe Asn Gly Met Arg 2180 2185 2190 gcc aat ggc agc ttg gca gga tta cat gtg ccg ccc gat gga agc atc 6624 Ala Asn Gly Ser Leu Ala Gly Leu His Val Pro Pro Asp Gly Ser Ile 2195 2200 2205 ctg ttc agg gcc gga aat gcc gaa aga acc gta atc agc tat gac atg 6672 Leu Phe Arg Ala Gly Asn Ala Glu Arg Thr Val Ile Ser Tyr Asp Met 2210 2215 2220 gac agc cat gat ttg gat aca tta tca ttc agg gaa tca ttc aaa cca 6720 Asp Ser His Asp Leu Asp Thr Leu Ser Phe Arg Glu Ser Phe Lys Pro 2225 2230 2235 2240 gat gtc gga cag tcg aca ccc aac ata agg gac atg gac ata tcc ccg 6768 Asp Val Gly Gln Ser Thr Pro Asn Ile Arg Asp Met Asp Ile Ser Pro 2245 2250 2255 gac ggc atg ttc ctc tac ctg ctt caa ggc gat gtt ctg gac atg tac 6816 Asp Gly Met Phe Leu Tyr Leu Leu Gln Gly Asp Val Leu Asp Met Tyr 2260 2265 2270 aac ctt aca gat agt tat tcg ctt gat gcc ccg gca tat gcg ggt acc 6864 Asn Leu Thr Asp Ser Tyr Ser Leu Asp Ala Pro Ala Tyr Ala Gly Thr 2275 2280 2285 ctg gat ttg gaa ccg gag gat gta ata ccc agg ggg att tca ttc tca 6912 Leu Asp Leu Glu Pro Glu Asp Val Ile Pro Arg Gly Ile Ser Phe Ser 2290 2295 2300 cgg gat ggc acg agt ctg ttt atg aca ggc gaa gac gtg gac cac att 6960 Arg Asp Gly Thr Ser Leu Phe Met Thr Gly Glu Asp Val Asp His Ile 2305 2310 2315 2320 cac gaa tat gca ttg aat gaa cca tgg gac ata cgc aat gcc ata ctt 7008 His Glu Tyr Ala Leu Asn Glu Pro Trp Asp Ile Arg Asn Ala Ile Leu 2325 2330 2335 gca ggc tcc ctg tcc ata agc gca gtg aat ggt gca ccg cgg ggg ctg 7056 Ala Gly Ser Leu Ser Ile Ser Ala Val Asn Gly Ala Pro Arg Gly Leu 2340 2345 2350 gat ata tcg gag gat ggc aca act gca cat act atg cgc ggg cgt gac 7104 Asp Ile Ser Glu Asp Gly Thr Thr Ala His Thr Met Arg Gly Arg Asp 2355 2360 2365 ttt gac acg ggg ccc gca tcc ctg gta aac cac ata ttg cca ggc caa 7152 Phe Asp Thr Gly Pro Ala Ser Leu Val Asn His Ile Leu Pro Gly Gln 2370 2375 2380 tat tcc ctg ctg acg gat gcg ccg gcg ttt gca tac ccc gtg gag gag 7200 Tyr Ser Leu Leu Thr Asp Ala Pro Ala Phe Ala Tyr Pro Val Glu Glu 2385 2390 2395 2400 gag ggt gca ccg ggg gat ctt gca ttc tcc gat gac ggc atg cgc atg 7248 Glu Gly Ala Pro Gly Asp Leu Ala Phe Ser Asp Asp Gly Met Arg Met 2405 2410 2415 ttc gtg gcg ggc gta aac aac cat tta aga cag tac aac ctg ctg tcg 7296 Phe Val Ala Gly Val Asn Asn His Leu Arg Gln Tyr Asn Leu Leu Ser 2420 2425 2430 ccg tat gac act gaa aat gca gaa cat ttc atc tcg acg gat ctg ctg 7344 Pro Tyr Asp Thr Glu Asn Ala Glu His Phe Ile Ser Thr Asp Leu Leu 2435 2440 2445 act gcg gac agg ggc ccc acg ggt ctt gta ttt tca gat gag aac gac 7392 Thr Ala Asp Arg Gly Pro Thr Gly Leu Val Phe Ser Asp Glu Asn Asp 2450 2455 2460 ttt ttc agc aca ggc gcc agg gcc caa ttt gtg cgc cag ttt acg aca 7440 Phe Phe Ser Thr Gly Ala Arg Ala Gln Phe Val Arg Gln Phe Thr Thr 2465 2470 2475 2480 aac cgc ccg tac gac gca tcc aca ata aca ctg agt gac aac gga ctg 7488 Asn Arg Pro Tyr Asp Ala Ser Thr Ile Thr Leu Ser Asp Asn Gly Leu 2485 2490 2495 tac aag gtg agc gtg gac ggc ctg ccg tcc ggc ata cgg ttt acc ccc 7536 Tyr Lys Val Ser Val Asp Gly Leu Pro Ser Gly Ile Arg Phe Thr Pro 2500 2505 2510 gac ggc atg aag atg ttc ata tcg ggc cag gag acg gcc atg ata tac 7584 Asp Gly Met Lys Met Phe Ile Ser Gly Gln Glu Thr Ala Met Ile Tyr 2515 2520 2525 cag tat tcc ctg ccg tcc ccg tat gac aca tcc ggg gcg gtc agg gac 7632 Gln Tyr Ser Leu Pro Ser Pro Tyr Asp Thr Ser Gly Ala Val Arg Asp 2530 2535 2540 agg gtt gag ata gtc gca ggg ctc ttt aga aat gca ggt ttg tcc gtc 7680 Arg Val Glu Ile Val Ala Gly Leu Phe Arg Asn Ala Gly Leu Ser Val 2545 2550 2555 2560 ggg ttg aac gag ccc agt cct tcc ggc ttt gac ttt tcg gag gac gga 7728 Gly Leu Asn Glu Pro Ser Pro Ser Gly Phe Asp Phe Ser Glu Asp Gly 2565 2570 2575 atg gag ctg tac gtg acg ggg tcg ggc ctt gtt cac agg tat ttc ctg 7776 Met Glu Leu Tyr Val Thr Gly Ser Gly Leu Val His Arg Tyr Phe Leu 2580 2585 2590 cca tcg cca tac ggc ctc gaa gat gca gcg tac ggg ggc agc ttc cac 7824 Pro Ser Pro Tyr Gly Leu Glu Asp Ala Ala Tyr Gly Gly Ser Phe His 2595 2600 2605 acg ttc agg gag agc acg ccg ctg gga gtg gtg gtg cgg ggg gat gcc 7872 Thr Phe Arg Glu Ser Thr Pro Leu Gly Val Val Val Arg Gly Asp Ala 2610 2615 2620 atg ttt gtg gcc ggg gac agt act gat tcc ata ttg aaa tat tcc ctg 7920 Met Phe Val Ala Gly Asp Ser Thr Asp Ser Ile Leu Lys Tyr Ser Leu 2625 2630 2635 2640 aac gca caa cct gtc ggc aac ata acc cat gcc gat acg cgc gcc ggg 7968 Asn Ala Gln Pro Val Gly Asn Ile Thr His Ala Asp Thr Arg Ala Gly 2645 2650 2655 att gcc gac agg gcg gag atc gtg ttt ggg gca atg gca gat acg cgc 8016 Ile Ala Asp Arg Ala Glu Ile Val Phe Gly Ala Met Ala Asp Thr Arg 2660 2665 2670 gcc gag att ctc gac ggc gcc gat gta gtt cat aag agt gtg aaa att 8064 Ala Glu Ile Leu Asp Gly Ala Asp Val Val His Lys Ser Val Lys Ile 2675 2680 2685 gac gta ttc cca ata tcg gag ggc ata aca gtg ggc agg gca ctt tat 8112 Asp Val Phe Pro Ile Ser Glu Gly Ile Thr Val Gly Arg Ala Leu Tyr 2690 2695 2700 cca gag gac gcc gcc ata ctt gat gac ggc gcg aat gcc acg cat aat 8160 Pro Glu Asp Ala Ala Ile Leu Asp Asp Gly Ala Asn Ala Thr His Asn 2705 2710 2715 2720 agg gtt gta atc att gtt cac gac ata aca gaa ggc gat gcg ccg tcc 8208 Arg Val Val Ile Ile Val His Asp Ile Thr Glu Gly Asp Ala Pro Ser 2725 2730 2735 ata cat gat gag ccg att gcc gtg ggg att tac gcc ctc ggc cct atg 8256 Ile His Asp Glu Pro Ile Ala Val Gly Ile Tyr Ala Leu Gly Pro Met 2740 2745 2750 gat aca atc gcc gtg gtt gat ctc cac cgc ctg gcc gta tcc gca tcc 8304 Asp Thr Ile Ala Val Val Asp Leu His Arg Leu Ala Val Ser Ala Ser 2755 2760 2765 ttg tcc ggg ggt gat tcc ccg tcg gcc tca gat gca tcc gga gta gtg 8352 Leu Ser Gly Gly Asp Ser Pro Ser Ala Ser Asp Ala Ser Gly Val Val 2770 2775 2780 gcc gag agc cgc aga aac gcg gtg gac agg cct ggc gtg gaa gag cgc 8400 Ala Glu Ser Arg Arg Asn Ala Val Asp Arg Pro Gly Val Glu Glu Arg 2785 2790 2795 2800 ata gga cat ggt gta tcc ctg gag gcg gcc gac agg cct gcc gtc gac 8448 Ile Gly His Gly Val Ser Leu Glu Ala Ala Asp Arg Pro Ala Val Asp 2805 2810 2815 aac atg atg gat acg gat agt gcc ggc gtg tac gac cgc agt ccg gac 8496 Asn Met Met Asp Thr Asp Ser Ala Gly Val Tyr Asp Arg Ser Pro Asp 2820 2825 2830 gac ggg ccc gcc gta tcc gac agg tcc gcg ctg ggg ctt gcc cgg atg 8544 Asp Gly Pro Ala Val Ser Asp Arg Ser Ala Leu Gly Leu Ala Arg Met 2835 2840 2845 gca gcc gac agg cct gca gtc gat gac atg atg gat acg gat agt gcc 8592 Ala Ala Asp Arg Pro Ala Val Asp Asp Met Met Asp Thr Asp Ser Ala 2850 2855 2860 ggc gtg tac gac cgc agc ccg gac gac ggg ccc gcc ata tcc gac agg 8640 Gly Val Tyr Asp Arg Ser Pro Asp Asp Gly Pro Ala Ile Ser Asp Arg 2865 2870 2875 2880 tcc gcg ctg ggg ctt gcc cgg atg gca gcc gac agg cct gca gtc gac 8688 Ser Ala Leu Gly Leu Ala Arg Met Ala Ala Asp Arg Pro Ala Val Asp 2885 2890 2895 gac atg atg gat acg ggc agt gcc ggc gtg tac gac cgc agc ccg gac 8736 Asp Met Met Asp Thr Gly Ser Ala Gly Val Tyr Asp Arg Ser Pro Asp 2900 2905 2910 gac ggg ccc gcc ata tcc gac agg tcc gcg ctg ggg ctt gcc cgg atg 8784 Asp Gly Pro Ala Ile Ser Asp Arg Ser Ala Leu Gly Leu Ala Arg Met 2915 2920 2925 gca gcc gac agg cct gca gtc gat gac atg atg gat acg ggc agt gag 8832 Ala Ala Asp Arg Pro Ala Val Asp Asp Met Met Asp Thr Gly Ser Glu 2930 2935 2940 agc acg agc agg ctt gga ccg gtt gac agg cca gaa ata gtc gag cgc 8880 Ser Thr Ser Arg Leu Gly Pro Val Asp Arg Pro Glu Ile Val Glu Arg 2945 2950 2955 2960 cac agc ctg gcc gcg tct gta tac ctg tcc ggg ggc gat tcc ccg tcg 8928 His Ser Leu Ala Ala Ser Val Tyr Leu Ser Gly Gly Asp Ser Pro Ser 2965 2970 2975 gtc gca gac ggt cat gat gtg gag tcc gag ggc cgc aga gac ggg ggg 8976 Val Ala Asp Gly His Asp Val Glu Ser Glu Gly Arg Arg Asp Gly Gly 2980 2985 2990 gac agg cct ggc atc gac gag cgt ata gtc atc aag atc tcg tac agc 9024 Asp Arg Pro Gly Ile Asp Glu Arg Ile Val Ile Lys Ile Ser Tyr Ser 2995 3000 3005 cgc ggc gca gcc gat gcg ccc aga gtg gag gat gca atg gag act tcc 9072 Arg Gly Ala Ala Asp Ala Pro Arg Val Glu Asp Ala Met Glu Thr Ser 3010 3015 3020 ggc gtg acc gcg tac agc cgc ggc gca gcc gat gcg ccc aga gtg gag 9120 Gly Val Thr Ala Tyr Ser Arg Gly Ala Ala Asp Ala Pro Arg Val Glu 3025 3030 3035 3040 gat gca atg gag act tcc ggc gtg acc gtc ccc agg cgc agt acc atg 9168 Asp Ala Met Glu Thr Ser Gly Val Thr Val Pro Arg Arg Ser Thr Met 3045 3050 3055 gac gcg ccc aca gtg gcc gat gac cac agc ctg gcc cgg acc gca tcc 9216 Asp Ala Pro Thr Val Ala Asp Asp His Ser Leu Ala Arg Thr Ala Ser 3060 3065 3070 ata tcc gaa ggc gat tcc ccg aca ttt gca gag gcg cgc cgc gcg gat 9264 Ile Ser Glu Gly Asp Ser Pro Thr Phe Ala Glu Ala Arg Arg Ala Asp 3075 3080 3085 acc gtt ggg gat ata gac gag gtg gac gcg ccc aca gtg gcc gat gac 9312 Thr Val Gly Asp Ile Asp Glu Val Asp Ala Pro Thr Val Ala Asp Asp 3090 3095 3100 cac agt ctg gcc cgg gcc gca tcc ata tcc gaa ggc gat tcc ccg aca 9360 His Ser Leu Ala Arg Ala Ala Ser Ile Ser Glu Gly Asp Ser Pro Thr 3105 3110 3115 3120 ttt gca gag gtg cgc cgc gcg gat acc gtt ggg gat ata gac gag gtg 9408 Phe Ala Glu Val Arg Arg Ala Asp Thr Val Gly Asp Ile Asp Glu Val 3125 3130 3135 gac gcg ccc gcc gtg gcc gag agg ctc ctg gca gtc ctc ggc ctg cag 9456 Asp Ala Pro Ala Val Ala Glu Arg Leu Leu Ala Val Leu Gly Leu Gln 3140 3145 3150 gcc cct gat tcg ccg gga gtg tgg gat act gta gga ata gat cac tcg 9504 Ala Pro Asp Ser Pro Gly Val Trp Asp Thr Val Gly Ile Asp His Ser 3155 3160 3165 gag att tca ggc gat cct gtg ccg gag cca aga gta gtg ccc agg ggc 9552 Glu Ile Ser Gly Asp Pro Val Pro Glu Pro Arg Val Val Pro Arg Gly 3170 3175 3180 ggt ggc ggt ggg gga ggc ggt tct tcg aac cgc ggc ctt gaa ccg cat 9600 Gly Gly Gly Gly Gly Gly Gly Ser Ser Asn Arg Gly Leu Glu Pro His 3185 3190 3195 3200 ggc ggc ggg tat gag att gac ttt gag ttc cgc ata gac ggc agg ctg 9648 Gly Gly Gly Tyr Glu Ile Asp Phe Glu Phe Arg Ile Asp Gly Arg Leu 3205 3210 3215 gtg ctc ttc aat ggg aca gac gtg cta gcc gaa tcc ggc aag gac ctg 9696 Val Leu Phe Asn Gly Thr Asp Val Leu Ala Glu Ser Gly Lys Asp Leu 3220 3225 3230 ctc atc cgt ccg gtg ttc cgg ccg gag ggg agt ttc aac ata ttt gat 9744 Leu Ile Arg Pro Val Phe Arg Pro Glu Gly Ser Phe Asn Ile Phe Asp 3235 3240 3245 atg gag gtg ttg ttt acc gcc ccc ggc ggg gag ata tcg act gcc tac 9792 Met Glu Val Leu Phe Thr Ala Pro Gly Gly Glu Ile Ser Thr Ala Tyr 3250 3255 3260 tac aac agg gct gga atc ctc atg ggg att gac tgc ggc gag ctg att 9840 Tyr Asn Arg Ala Gly Ile Leu Met Gly Ile Asp Cys Gly Glu Leu Ile 3265 3270 3275 3280 atg acc gat acg acg tat tca tgc gac atg ctg gac ata ttc gga gat 9888 Met Thr Asp Thr Thr Tyr Ser Cys Asp Met Leu Asp Ile Phe Gly Asp 3285 3290 3295 gag ata tac cat gtg gag agg ctt gac gca ttc aac ggc atg gtc atc 9936 Glu Ile Tyr His Val Glu Arg Leu Asp Ala Phe Asn Gly Met Val Ile 3300 3305 3310 tcc ttg gac ggc ccc ctc gac ggg acg gtc agt gta tcg ctt cgt gac 9984 Ser Leu Asp Gly Pro Leu Asp Gly Thr Val Ser Val Ser Leu Arg Asp 3315 3320 3325 aac cac ggc atc ccg ctg gcg cag cat cgg ctg cat aaa tac gag att 10032 Asn His Gly Ile Pro Leu Ala Gln His Arg Leu His Lys Tyr Glu Ile 3330 3335 3340 ttg att ttg gac gcc gct gaa aac aga ccc ctg tca gtc tcg acg gac 10080 Leu Ile Leu Asp Ala Ala Glu Asn Arg Pro Leu Ser Val Ser Thr Asp 3345 3350 3355 3360 ccc aag ccc gtg gag gat cca tcg ccc gtg cag cat ata gag tcc ctc 10128 Pro Lys Pro Val Glu Asp Pro Ser Pro Val Gln His Ile Glu Ser Leu 3365 3370 3375 cag atg gat ccg gag ccc gtg gag tcc gag ccc ctc ccg atg gac tcc 10176 Gln Met Asp Pro Glu Pro Val Glu Ser Glu Pro Leu Pro Met Asp Ser 3380 3385 3390 gag ccc gtg gag gat ctg gaa cct gtg cag cat cta gag tcc ctc ccg 10224 Glu Pro Val Glu Asp Leu Glu Pro Val Gln His Leu Glu Ser Leu Pro 3395 3400 3405 atg gac ccc gag ccc gtg gag gat ctg gaa cct gtg cag cat ctc gag 10272 Met Asp Pro Glu Pro Val Glu Asp Leu Glu Pro Val Gln His Leu Glu 3410 3415 3420 ccc gtg cag gga tcc ccg ccc gtg cag gga ggg ccg gag tcc gtg gag 10320 Pro Val Gln Gly Ser Pro Pro Val Gln Gly Gly Pro Glu Ser Val Glu 3425 3430 3435 3440 tca ggc ata gca tac acg cta tgg cag ttc ctt tca gga ctg ctg gat 10368 Ser Gly Ile Ala Tyr Thr Leu Trp Gln Phe Leu Ser Gly Leu Leu Asp 3445 3450 3455 gcc ctg ggt ctt gcc gac ccg gat gtc gga tct gtc caa aaa acg tcc 10416 Ala Leu Gly Leu Ala Asp Pro Asp Val Gly Ser Val Gln Lys Thr Ser 3460 3465 3470 tga 10419 <210> SEQ ID NO 4 <211> LENGTH: 3472 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 4 Met Pro Ala Pro Pro Gly Glu Gly Ser Leu Gly Gly Val Ala Ile Ser 1 5 10 15 Asp Asp Gly Arg Tyr Met Tyr Ala Ile Gly Arg Asp Leu Leu Thr Val 20 25 30 Tyr Arg Tyr Thr Met Asn Pro Pro His Asp Ile Ala Ser Ala Ala Leu 35 40 45 Gly Ala Gln Ser Phe Ser Leu Pro Gly Gly Ile Ser Pro Ala Pro Gly 50 55 60 Ala Pro Thr Gly Leu Asp Ile Ser Asp Asp Gly Arg His Leu Tyr Val 65 70 75 80 Pro Asp Glu Asn Gly Val Val Tyr Arg Phe Asp Leu Glu Ser Pro Tyr 85 90 95 Arg Leu Asp Gly Gly Thr Phe Gly Ser Ser Val Tyr Val Gly Ser Asp 100 105 110 Val Ala Ala Pro Arg Gly Val Tyr Val Ala Pro Gly Gly Ser Leu Met 115 120 125 Leu Val Ser Asp Ser Ala Asp Gly Thr Ile His Arg Tyr Glu Leu Ala 130 135 140 Ser Pro Tyr Glu Pro Ala Gly Ala Ala Asn Arg Gly Ser Phe Asp Val 145 150 155 160 Ser Asp Met Asp Gly Ser Pro Val Gly Ala Gly Phe Ala Gly Gly Leu 165 170 175 His Met Tyr Val Ala Gly Asn Asp Thr Gly Arg Val Tyr Gln Tyr Pro 180 185 190 Ala Gly Thr His Gln Ile Gln Glu Ala Ala Ala Gly Pro Arg Leu Leu 195 200 205 Ser Ala Val Leu Asp Lys Asp Gly Thr Leu Arg Ala Ala Phe Asp Gly 210 215 220 Thr Val Asp Ala Gly Ser Val Gln Pro Gly Met Ile Thr Ile Arg Asp 225 230 235 240 Gly His Gly Ser Asn Thr Gly Ile Pro Leu Leu Leu Ala Gly Gly Ala 245 250 255 Ala Asp Ser Asp Val Met Thr Phe Val Val Pro Glu Lys Asp Arg Ala 260 265 270 Glu Ala Ala Ala Tyr Gly Asp Gln Ser Leu His Val Pro Ala Ala Ala 275 280 285 Leu Ala Gly Thr Gly Gly Gly Pro Phe Val Pro Asp Phe Ser Gly Gly 290 295 300 Ser Leu Leu Ala Ser Leu Tyr Arg His Glu Arg Pro Phe Gln Gly Glu 305 310 315 320 Glu Met Ala Arg Thr Glu Arg Ser Asp Arg Tyr Ala Leu Thr Val Thr 325 330 335 Ala Gly Gly Ser Gln Met His Val Gly Gly Ala Gly Gly Asn Ile Thr 340 345 350 Trp Tyr Asp Leu Gly Thr Pro His Asp Ile Thr Thr Gly Val Arg Ala 355 360 365 Gly Ser Asp Ile Leu Pro Ala Tyr Pro Ser Ala Gly Arg Asn Val Val 370 375 380 Pro Ser Ile Thr Gly Ile Ala Phe Ser Asp Asp Gly Met Arg Leu Phe 385 390 395 400 Ala Ala Asn Arg Gly Asp Arg Ile Pro Met Tyr Gln Leu Asp Ser Pro 405 410 415 Tyr Asp Ile Gly Ser Ala Ser Leu Glu Gly Thr Leu Phe Thr Gly Phe 420 425 430 Gln Ser Gly Ile Ala Phe Ser Asp Asp Gly Thr Arg Met Phe Ala Ala 435 440 445 Leu Leu Thr Glu Asn Ala Ile Arg Gln Tyr Asp Leu Glu Gly Pro Tyr 450 455 460 Asp Ile Arg Gly Ala Gly Asn Ala Gly Gln Tyr Asp Leu Asp Ile Pro 465 470 475 480 Leu His Pro Gly Leu Leu Phe Leu Leu Thr Ser Gly Val His Phe Ser 485 490 495 Pro Asp Gly Thr Arg Met Phe Val Gly Glu Gly Ile Ser Asp Ala Glu 500 505 510 Asp Ala Asn Ala Asn Arg Asp Val Asn Val Asn Leu Trp His Arg Phe 515 520 525 Asp Leu Ser Thr Pro Phe Asp Val Leu Thr Ala Glu Arg Val Asp Thr 530 535 540 Tyr Glu Tyr Ser Thr Gly Pro Ala Gly Asp Leu Glu Asp Leu Ser Leu 545 550 555 560 Ser Pro Asp Gly Arg Arg Leu Tyr Thr Leu Ser Ser Glu Arg Val Ser 565 570 575 Ser Ser Glu Tyr Thr Ile Thr Arg Ala Gln Tyr Trp Leu Pro Glu Pro 580 585 590 Tyr Asp Val Thr Pro Pro Tyr His Val Pro Ser Phe Asn Ala Ser Gln 595 600 605 Gly Gly Asn Leu Ala Asp Pro Tyr Gly Met Ala Phe Ser Pro Asp Gly 610 615 620 Thr Arg Leu Leu Val Thr Gly His Gly Gln Thr Asn Ala Lys Leu Phe 625 630 635 640 His Leu Asn Pro Pro Phe Asp Val Gly Thr Ala Val Phe His Asp His 645 650 655 Gly Arg Phe Arg Pro Gly Gly Pro Ala Ser Glu Ile Glu Ala Ser Gly 660 665 670 Ile Ser Leu Ser Ala Asp Gly Ser Arg Met Phe Leu Ser Asp Arg Gly 675 680 685 Arg Gly Ala Ile Ser Gln Tyr Thr Leu Val Ala Pro Phe Asp Val Glu 690 695 700 Phe Ala Ser Asp Val Ser Ala Asp Gly Gln Leu Asp Val Gly Ala Gln 705 710 715 720 Asp Ala Leu Pro Gly Gly Leu Ala Phe Ser Pro Gly Gly Thr Arg Leu 725 730 735 Phe Met Val Gly Gly Met Asp Arg Ser Val His Met Tyr Ser Leu Asn 740 745 750 Thr Pro Phe Asp Leu Gly Gly Ala Glu His Ala Ala Ser Phe Gly Val 755 760 765 Gly Asp Arg Val Ser Asp Pro Leu Gly Ile Ala Phe Gly Asn Gly Gly 770 775 780 Thr Lys Met Leu Ile Ala Asp Thr Thr Gly Phe Val His Gly Tyr Asp 785 790 795 800 Leu Gly Ala Pro Tyr Asp Ile Ser Gly Pro Ala Tyr Ser Gly Ile Phe 805 810 815 Asp Ala Gly Gly Ser Ile Arg Asp Val Ala Val Gly Gly Gly Ser Met 820 825 830 Phe Ile Leu Glu Gly Glu Thr Asp Arg Val Tyr Glu His Arg Pro Gly 835 840 845 Ile Tyr Pro Val Val Ser Ala Leu Asp Gly Pro Ala Leu Val Ser Ala 850 855 860 Ala Ala Asp Ala Arg Val Gly Ala Ala Glu Val Leu Phe Asp Arg Ala 865 870 875 880 Val Asp Val Gly Gly Ile Asp Pro Gly Gly Val Arg Ile Val Asp Ala 885 890 895 Ala Gly Pro Leu Pro Gly Val Val Ile Ser Asp Ala Val Ile Pro Gly 900 905 910 Glu Asp Pro Gly Val Ala Arg Phe Ser Leu Ser Asp Ala Glu Val Leu 915 920 925 Ala Val Ser Gly Tyr Ala Glu Pro Ser Leu Val Phe Gly Arg His Ala 930 935 940 Val Pro Gly Ala Ala Gly Gly Thr Phe Pro Ser Gln Ile Gly Asn Ala 945 950 955 960 Thr Glu Leu Val Gly Ser Ile Pro Asn Pro Thr Leu Asp Phe Gly Thr 965 970 975 Thr Leu Thr Gly Ala Ala Phe Ser Ala Asp Gly Thr Val Val Phe Leu 980 985 990 Ser Asp Gly Pro Thr Gly Arg Val Tyr Pro Tyr Ser Leu Asn Ile Pro 995 1000 1005 Phe Asp Ile Ser Ser Ala Ala Pro Gly Gly Phe Val Ile Val Pro Val 1010 1015 1020 Gly Val Ser Asp Ile Ala Phe Ser Ala Asp Gly Arg Asn Met Leu Val 1025 1030 1035 1040 Ala Asp Glu Thr Gly Gly Ile His Arg Tyr Leu Ala Arg Ser Pro Tyr 1045 1050 1055 Glu Ile Gly Thr Asp Phe Ile Lys Ser Ser Leu Gly Glu Phe Val Glu 1060 1065 1070 Thr Phe Ser Ala Ala Pro Arg Val Gln Asp Leu Ala Gly Ile Ala Phe 1075 1080 1085 Ser His Asp Gly Met Ile Met Leu Ala Ala Gly Gly Ser Gly Ser Val 1090 1095 1100 His Arg Tyr Ser Leu Pro Ser Pro Tyr Ala Val Ser Gly Ala Lys Tyr 1105 1110 1115 1120 Glu Glu Thr Ala Met Ile Gly Gly Ser Pro Ser Gly Leu Glu Phe Ser 1125 1130 1135 Ser Asp Gly Leu Arg Met Phe Val Pro Asp Ala Gly Ser Glu Thr Ala 1140 1145 1150 Ala Val Tyr Gly Leu Ala Ala Pro Tyr Gly Ile Gly Glu Ala Glu Pro 1155 1160 1165 Leu Pro Pro Leu Phe Leu Gly Val Gly Ala Glu Glu Ala Thr Leu Ser 1170 1175 1180 Pro Asp Gly Arg His Ile Leu Val Pro Gly Arg Pro Gly Leu Ser Gln 1185 1190 1195 1200 Tyr Ser Leu Phe Ser Thr Asn Leu Glu Leu Cys Ala Glu Pro Arg Gly 1205 1210 1215 Ile Asp Gly Gly Ser Cys Glu Asp Gly Ile Tyr Ala Phe Glu Ser Pro 1220 1225 1230 Gly Arg Gly Glu Gly Val Ser Leu Ala Ala Ser Ile Thr Ala Ala Asp 1235 1240 1245 Gly Pro Gly Ile Gly Glu Leu His Gly Phe Ala Gly Pro Pro Met Pro 1250 1255 1260 Ala Pro Val Met Glu Gln Val Thr Leu Asp Ser Arg Glu Gly Thr Leu 1265 1270 1275 1280 Arg Val Arg Leu Asp Arg Thr Val Asp Val Asp Thr Val Arg Pro Tyr 1285 1290 1295 Lys Met Trp Val Glu Asp Ser Asp Gly Ser Gln Thr Thr Leu Ala Asn 1300 1305 1310 Ser Thr Leu Leu Asn Ala Glu Asn Ser Asn Ile Leu Leu Phe Arg Leu 1315 1320 1325 Asp Asp Ala Ala Ala Gly Lys Ile Ser Gly Tyr Thr Ser Pro Val Phe 1330 1335 1340 Arg Thr Trp Ser Ser Pro Phe Leu Gly Thr Asp Gly Ala Thr Arg Pro 1345 1350 1355 1360 His Thr Leu Gly Phe Gly Asp Val Arg Leu Ala Asp Ile Tyr Asp Ala 1365 1370 1375 Ser Gly Asp Val Pro Ser Pro Ser Gly Ile Glu Phe Ser Asp Asp Gly 1380 1385 1390 Met Arg Met Phe Val Thr Gly Ile Gly Thr Pro Gly Ile Asn Ile Phe 1395 1400 1405 Thr Leu Ser Ala Pro Phe Asp Ile Thr Leu Pro Lys His Ser Gly Ser 1410 1415 1420 Thr Asn Ile Gly Gly Leu Ser Val Ser Asp Leu Ala Phe Ala Asn Asn 1425 1430 1435 1440 Gly Asn Ser Leu Thr Val Leu Asp Val Asp Gly Val Leu Arg Val Tyr 1445 1450 1455 Ala Leu Gly Asp Asp Tyr Asn Val Val Thr Gly Thr Thr Gln Lys Phe 1460 1465 1470 Arg Ile Thr Leu Asp Thr Thr Gln Gly Ile Pro Asn Ser Ile Tyr Thr 1475 1480 1485 Ser Pro Asp Gly Leu Ser Gln Phe Val Ala Tyr Asp Asp Arg Ile Asp 1490 1495 1500 Leu Tyr Val Leu Gly Ser Pro Asn Asp Ile Ser Ser Thr Thr Glu Ile 1505 1510 1515 1520 Ile Pro Tyr Ser Leu Pro Arg Pro Asp Pro Pro Thr Gly Met Asp Phe 1525 1530 1535 Thr Pro Asp Gly Arg Arg Met Phe Leu Ser Thr Glu Asn Gly Ile Asp 1540 1545 1550 Gln Tyr Leu Leu Ser Glu Pro Phe Ala Val Thr Thr Ser Val Phe Leu 1555 1560 1565 Arg Thr Ile Pro Ile Asp Gly Gly Ala Glu Gly Ile Arg Phe Val Asp 1570 1575 1580 Asn Gly Arg Gly Leu Phe Val Pro Gly Ala Asp Gly Ile Ile Gln Arg 1585 1590 1595 1600 His Glu Leu Ile Tyr Pro Tyr Gly Ala Ser Thr Ser Leu Leu Glu Thr 1605 1610 1615 Val Arg Asp Gly Val Thr Asp Gly Gly Pro Gly Glu Asn Pro Ala Ala 1620 1625 1630 Gly Glu Ile Arg Leu Ala Gly Thr Phe Asn Ala Ser Asp Asn Val Gln 1635 1640 1645 Ser Pro Ser Gly Ile Glu Phe Ser Gly Asp Gly Thr Gly Met Phe Val 1650 1655 1660 Thr Gly Phe Gly Ala Ala Gly Val Asn Glu Phe Ser Leu Ser Ala Pro 1665 1670 1675 1680 Phe Asp Thr Thr Leu Pro Val His Val Glu Leu His Asp Ile Gly Gly 1685 1690 1695 Gln Pro Ala Val Asp Leu Ala Phe Ala Glu Asp Gly Arg Thr Leu Leu 1700 1705 1710 Leu Leu Ala Ala Asp Gly Thr Leu Asp Phe Tyr Ser Leu Ala Gly Asp 1715 1720 1725 Ala Tyr Asp Ile Gly Glu Ala Ser Arg Thr Phe Gln Val Pro Phe Glu 1730 1735 1740 Asp Ala Ala Gly Ala Val Pro Gly Ala Phe Tyr Gln Pro Pro Asp Gly 1745 1750 1755 1760 Ser Ser Ile Ile Ala Ala Phe Asp Gly Arg Ile Asp Gln Tyr Val Val 1765 1770 1775 Ile Pro Phe Glu Phe Val Ser Tyr Pro Leu Thr Arg Pro Gly Thr Pro 1780 1785 1790 Thr Gly Ile Asp Phe Ala Pro Asp Gly Arg Trp Met Phe Leu Ser Thr 1795 1800 1805 Glu Asn Gly Ile Asp Gln Tyr Leu Leu Ser Ile Pro Phe Asp Val Arg 1810 1815 1820 Ser Leu Thr Tyr Thr Gly Thr Ile Pro Val Asp Gly Val Glu Gly Met 1825 1830 1835 1840 Gln Phe Ala Asp Asn Gly Arg Ala Leu Phe Leu Ala Asp Ser Glu Gly 1845 1850 1855 Leu Ile Tyr Asn Tyr Asp Leu Glu Asp Pro Tyr Ala Leu Asp Gly Asn 1860 1865 1870 Thr Ile Ser Val Glu Phe Ser Phe Asp Gly Ser Val Met Tyr Val Leu 1875 1880 1885 Glu Tyr Asp Thr Lys Arg Val Val Ser Tyr Glu Leu Glu Phe Pro Phe 1890 1895 1900 Asp Val Ser Ser Arg Thr Arg Ala Asp Thr Leu Asp Ile Pro Gln Ile 1905 1910 1915 1920 Asp Ser Pro Arg His Val Ala Val Ser Met Pro Gly Asn His Leu Tyr 1925 1930 1935 Ile Thr Asn Ser Val Phe Gly Glu Asp Asp Thr Ile His Ser Tyr Gly 1940 1945 1950 Ile Ser Asn Asn Asp Ile Ser Ser Ala Ser Tyr Ile Gly Glu Glu Gly 1955 1960 1965 Ile Pro Glu Pro Val Ile Asn Gly Ile Asp Phe Ser Asn Asn Gly Arg 1970 1975 1980 Arg Met Phe Leu Ile Gly Gly Asn Gly Phe Asp Tyr Gln Val Ile His 1985 1990 1995 2000 Asp Tyr Met Leu Gly Thr Arg Tyr Asp Ile Ser Ser Arg Ser Leu Leu 2005 2010 2015 Asp Thr Tyr Ala Ile Pro Gly Pro Val Val Phe Pro Ala Gly Leu Asp 2020 2025 2030 Phe Ser Phe Asp Arg Leu Ser Met Phe Ile Ile Ser Thr Ala Gly Ser 2035 2040 2045 Val Tyr Arg Tyr Gly Leu Asp Asp Pro Phe Ile Val Glu Thr Met Asp 2050 2055 2060 Tyr Gln Glu Ser Phe Arg Leu Pro Val Pro Ser Ala Ala Asp Asn Ser 2065 2070 2075 2080 Ile Ser Asp Leu Ala Phe Gly Ser Ser Gly Leu Asn Ala Val Ile Ser 2085 2090 2095 His Glu Gly Leu Asp Thr Leu Tyr Ser Phe Val Leu Asp Ile Pro Tyr 2100 2105 2110 Gly Ala Glu Leu Asp Ile Asp Arg Leu Glu Leu Pro Leu Val Gly Val 2115 2120 2125 Pro Thr Gly Phe Glu Phe Ser Asp Asn Gly Arg Gln Leu Tyr Ile Gly 2130 2135 2140 Ala Phe Arg Asp Ser Gln Ser Ser Pro Gly Thr Leu Pro Ala Gly Leu 2145 2150 2155 2160 Gln Arg Tyr Glu Leu Gly Ile Pro Tyr Asp Leu Ala Ser Ala Val Phe 2165 2170 2175 Ala Gln Ser Leu Gly Ile Phe Asp Phe Pro Pro Phe Asn Gly Met Arg 2180 2185 2190 Ala Asn Gly Ser Leu Ala Gly Leu His Val Pro Pro Asp Gly Ser Ile 2195 2200 2205 Leu Phe Arg Ala Gly Asn Ala Glu Arg Thr Val Ile Ser Tyr Asp Met 2210 2215 2220 Asp Ser His Asp Leu Asp Thr Leu Ser Phe Arg Glu Ser Phe Lys Pro 2225 2230 2235 2240 Asp Val Gly Gln Ser Thr Pro Asn Ile Arg Asp Met Asp Ile Ser Pro 2245 2250 2255 Asp Gly Met Phe Leu Tyr Leu Leu Gln Gly Asp Val Leu Asp Met Tyr 2260 2265 2270 Asn Leu Thr Asp Ser Tyr Ser Leu Asp Ala Pro Ala Tyr Ala Gly Thr 2275 2280 2285 Leu Asp Leu Glu Pro Glu Asp Val Ile Pro Arg Gly Ile Ser Phe Ser 2290 2295 2300 Arg Asp Gly Thr Ser Leu Phe Met Thr Gly Glu Asp Val Asp His Ile 2305 2310 2315 2320 His Glu Tyr Ala Leu Asn Glu Pro Trp Asp Ile Arg Asn Ala Ile Leu 2325 2330 2335 Ala Gly Ser Leu Ser Ile Ser Ala Val Asn Gly Ala Pro Arg Gly Leu 2340 2345 2350 Asp Ile Ser Glu Asp Gly Thr Thr Ala His Thr Met Arg Gly Arg Asp 2355 2360 2365 Phe Asp Thr Gly Pro Ala Ser Leu Val Asn His Ile Leu Pro Gly Gln 2370 2375 2380 Tyr Ser Leu Leu Thr Asp Ala Pro Ala Phe Ala Tyr Pro Val Glu Glu 2385 2390 2395 2400 Glu Gly Ala Pro Gly Asp Leu Ala Phe Ser Asp Asp Gly Met Arg Met 2405 2410 2415 Phe Val Ala Gly Val Asn Asn His Leu Arg Gln Tyr Asn Leu Leu Ser 2420 2425 2430 Pro Tyr Asp Thr Glu Asn Ala Glu His Phe Ile Ser Thr Asp Leu Leu 2435 2440 2445 Thr Ala Asp Arg Gly Pro Thr Gly Leu Val Phe Ser Asp Glu Asn Asp 2450 2455 2460 Phe Phe Ser Thr Gly Ala Arg Ala Gln Phe Val Arg Gln Phe Thr Thr 2465 2470 2475 2480 Asn Arg Pro Tyr Asp Ala Ser Thr Ile Thr Leu Ser Asp Asn Gly Leu 2485 2490 2495 Tyr Lys Val Ser Val Asp Gly Leu Pro Ser Gly Ile Arg Phe Thr Pro 2500 2505 2510 Asp Gly Met Lys Met Phe Ile Ser Gly Gln Glu Thr Ala Met Ile Tyr 2515 2520 2525 Gln Tyr Ser Leu Pro Ser Pro Tyr Asp Thr Ser Gly Ala Val Arg Asp 2530 2535 2540 Arg Val Glu Ile Val Ala Gly Leu Phe Arg Asn Ala Gly Leu Ser Val 2545 2550 2555 2560 Gly Leu Asn Glu Pro Ser Pro Ser Gly Phe Asp Phe Ser Glu Asp Gly 2565 2570 2575 Met Glu Leu Tyr Val Thr Gly Ser Gly Leu Val His Arg Tyr Phe Leu 2580 2585 2590 Pro Ser Pro Tyr Gly Leu Glu Asp Ala Ala Tyr Gly Gly Ser Phe His 2595 2600 2605 Thr Phe Arg Glu Ser Thr Pro Leu Gly Val Val Val Arg Gly Asp Ala 2610 2615 2620 Met Phe Val Ala Gly Asp Ser Thr Asp Ser Ile Leu Lys Tyr Ser Leu 2625 2630 2635 2640 Asn Ala Gln Pro Val Gly Asn Ile Thr His Ala Asp Thr Arg Ala Gly 2645 2650 2655 Ile Ala Asp Arg Ala Glu Ile Val Phe Gly Ala Met Ala Asp Thr Arg 2660 2665 2670 Ala Glu Ile Leu Asp Gly Ala Asp Val Val His Lys Ser Val Lys Ile 2675 2680 2685 Asp Val Phe Pro Ile Ser Glu Gly Ile Thr Val Gly Arg Ala Leu Tyr 2690 2695 2700 Pro Glu Asp Ala Ala Ile Leu Asp Asp Gly Ala Asn Ala Thr His Asn 2705 2710 2715 2720 Arg Val Val Ile Ile Val His Asp Ile Thr Glu Gly Asp Ala Pro Ser 2725 2730 2735 Ile His Asp Glu Pro Ile Ala Val Gly Ile Tyr Ala Leu Gly Pro Met 2740 2745 2750 Asp Thr Ile Ala Val Val Asp Leu His Arg Leu Ala Val Ser Ala Ser 2755 2760 2765 Leu Ser Gly Gly Asp Ser Pro Ser Ala Ser Asp Ala Ser Gly Val Val 2770 2775 2780 Ala Glu Ser Arg Arg Asn Ala Val Asp Arg Pro Gly Val Glu Glu Arg 2785 2790 2795 2800 Ile Gly His Gly Val Ser Leu Glu Ala Ala Asp Arg Pro Ala Val Asp 2805 2810 2815 Asn Met Met Asp Thr Asp Ser Ala Gly Val Tyr Asp Arg Ser Pro Asp 2820 2825 2830 Asp Gly Pro Ala Val Ser Asp Arg Ser Ala Leu Gly Leu Ala Arg Met 2835 2840 2845 Ala Ala Asp Arg Pro Ala Val Asp Asp Met Met Asp Thr Asp Ser Ala 2850 2855 2860 Gly Val Tyr Asp Arg Ser Pro Asp Asp Gly Pro Ala Ile Ser Asp Arg 2865 2870 2875 2880 Ser Ala Leu Gly Leu Ala Arg Met Ala Ala Asp Arg Pro Ala Val Asp 2885 2890 2895 Asp Met Met Asp Thr Gly Ser Ala Gly Val Tyr Asp Arg Ser Pro Asp 2900 2905 2910 Asp Gly Pro Ala Ile Ser Asp Arg Ser Ala Leu Gly Leu Ala Arg Met 2915 2920 2925 Ala Ala Asp Arg Pro Ala Val Asp Asp Met Met Asp Thr Gly Ser Glu 2930 2935 2940 Ser Thr Ser Arg Leu Gly Pro Val Asp Arg Pro Glu Ile Val Glu Arg 2945 2950 2955 2960 His Ser Leu Ala Ala Ser Val Tyr Leu Ser Gly Gly Asp Ser Pro Ser 2965 2970 2975 Val Ala Asp Gly His Asp Val Glu Ser Glu Gly Arg Arg Asp Gly Gly 2980 2985 2990 Asp Arg Pro Gly Ile Asp Glu Arg Ile Val Ile Lys Ile Ser Tyr Ser 2995 3000 3005 Arg Gly Ala Ala Asp Ala Pro Arg Val Glu Asp Ala Met Glu Thr Ser 3010 3015 3020 Gly Val Thr Ala Tyr Ser Arg Gly Ala Ala Asp Ala Pro Arg Val Glu 3025 3030 3035 3040 Asp Ala Met Glu Thr Ser Gly Val Thr Val Pro Arg Arg Ser Thr Met 3045 3050 3055 Asp Ala Pro Thr Val Ala Asp Asp His Ser Leu Ala Arg Thr Ala Ser 3060 3065 3070 Ile Ser Glu Gly Asp Ser Pro Thr Phe Ala Glu Ala Arg Arg Ala Asp 3075 3080 3085 Thr Val Gly Asp Ile Asp Glu Val Asp Ala Pro Thr Val Ala Asp Asp 3090 3095 3100 His Ser Leu Ala Arg Ala Ala Ser Ile Ser Glu Gly Asp Ser Pro Thr 3105 3110 3115 3120 Phe Ala Glu Val Arg Arg Ala Asp Thr Val Gly Asp Ile Asp Glu Val 3125 3130 3135 Asp Ala Pro Ala Val Ala Glu Arg Leu Leu Ala Val Leu Gly Leu Gln 3140 3145 3150 Ala Pro Asp Ser Pro Gly Val Trp Asp Thr Val Gly Ile Asp His Ser 3155 3160 3165 Glu Ile Ser Gly Asp Pro Val Pro Glu Pro Arg Val Val Pro Arg Gly 3170 3175 3180 Gly Gly Gly Gly Gly Gly Gly Ser Ser Asn Arg Gly Leu Glu Pro His 3185 3190 3195 3200 Gly Gly Gly Tyr Glu Ile Asp Phe Glu Phe Arg Ile Asp Gly Arg Leu 3205 3210 3215 Val Leu Phe Asn Gly Thr Asp Val Leu Ala Glu Ser Gly Lys Asp Leu 3220 3225 3230 Leu Ile Arg Pro Val Phe Arg Pro Glu Gly Ser Phe Asn Ile Phe Asp 3235 3240 3245 Met Glu Val Leu Phe Thr Ala Pro Gly Gly Glu Ile Ser Thr Ala Tyr 3250 3255 3260 Tyr Asn Arg Ala Gly Ile Leu Met Gly Ile Asp Cys Gly Glu Leu Ile 3265 3270 3275 3280 Met Thr Asp Thr Thr Tyr Ser Cys Asp Met Leu Asp Ile Phe Gly Asp 3285 3290 3295 Glu Ile Tyr His Val Glu Arg Leu Asp Ala Phe Asn Gly Met Val Ile 3300 3305 3310 Ser Leu Asp Gly Pro Leu Asp Gly Thr Val Ser Val Ser Leu Arg Asp 3315 3320 3325 Asn His Gly Ile Pro Leu Ala Gln His Arg Leu His Lys Tyr Glu Ile 3330 3335 3340 Leu Ile Leu Asp Ala Ala Glu Asn Arg Pro Leu Ser Val Ser Thr Asp 3345 3350 3355 3360 Pro Lys Pro Val Glu Asp Pro Ser Pro Val Gln His Ile Glu Ser Leu 3365 3370 3375 Gln Met Asp Pro Glu Pro Val Glu Ser Glu Pro Leu Pro Met Asp Ser 3380 3385 3390 Glu Pro Val Glu Asp Leu Glu Pro Val Gln His Leu Glu Ser Leu Pro 3395 3400 3405 Met Asp Pro Glu Pro Val Glu Asp Leu Glu Pro Val Gln His Leu Glu 3410 3415 3420 Pro Val Gln Gly Ser Pro Pro Val Gln Gly Gly Pro Glu Ser Val Glu 3425 3430 3435 3440 Ser Gly Ile Ala Tyr Thr Leu Trp Gln Phe Leu Ser Gly Leu Leu Asp 3445 3450 3455 Ala Leu Gly Leu Ala Asp Pro Asp Val Gly Ser Val Gln Lys Thr Ser 3460 3465 3470 <210> SEQ ID NO 5 <211> LENGTH: 819 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(810) <400> SEQUENCE: 5 atg cat ggg atc gag ggc ggc cgg gga gat atg tcg gag aat ttt gtg 48 Met His Gly Ile Glu Gly Gly Arg Gly Asp Met Ser Glu Asn Phe Val 1 5 10 15 gcg ttt tgc gtg gcg tgc gcc agg gga gtc aca aag gac gag atg aag 96 Ala Phe Cys Val Ala Cys Ala Arg Gly Val Thr Lys Asp Glu Met Lys 20 25 30 tat gta gac ggg agg gtc ttc cac aaa gag tgc cat gca agg cac ggc 144 Tyr Val Asp Gly Arg Val Phe His Lys Glu Cys His Ala Arg His Gly 35 40 45 ggg cag atc cgc ttc ccc aac cca gag gtc gag cag cgc gtg gcc gag 192 Gly Gln Ile Arg Phe Pro Asn Pro Glu Val Glu Gln Arg Val Ala Glu 50 55 60 ctg aag gtg gac ctg ata cag atg aga aac cag ctg gcc gag atg aac 240 Leu Lys Val Asp Leu Ile Gln Met Arg Asn Gln Leu Ala Glu Met Asn 65 70 75 80 agg gcg tcg ggg gac gga ggg gtg cat tcc agc gcc acc tct gcg gcc 288 Arg Ala Ser Gly Asp Gly Gly Val His Ser Ser Ala Thr Ser Ala Ala 85 90 95 gag gcc gag cag cac agg gcc gag cta aag gta cag ctg gtg cag atg 336 Glu Ala Glu Gln His Arg Ala Glu Leu Lys Val Gln Leu Val Gln Met 100 105 110 aga aac cag ctg gcc gag atg aac agg aag gcc ccc gga aag ccg gca 384 Arg Asn Gln Leu Ala Glu Met Asn Arg Lys Ala Pro Gly Lys Pro Ala 115 120 125 cgg aaa aag gcc gca ggc aag act gca cgg aga aag agc ggc aag aag 432 Arg Lys Lys Ala Ala Gly Lys Thr Ala Arg Arg Lys Ser Gly Lys Lys 130 135 140 acg gtg cgc agg aag acc ggc aag agg act gcc ggt aag aag gcc ggg 480 Thr Val Arg Arg Lys Thr Gly Lys Arg Thr Ala Gly Lys Lys Ala Gly 145 150 155 160 gcg cgg agg aag act acg gtc aag agg acg gcg cgg agg aag acc acg 528 Ala Arg Arg Lys Thr Thr Val Lys Arg Thr Ala Arg Arg Lys Thr Thr 165 170 175 gca aag aag gca gcc ggc aga aag gcc ggg gcg cgc aga aag gcc aca 576 Ala Lys Lys Ala Ala Gly Arg Lys Ala Gly Ala Arg Arg Lys Ala Thr 180 185 190 gtc aag agg acg gtg cac aaa aag att gga gtg cgg agg aag act acg 624 Val Lys Arg Thr Val His Lys Lys Ile Gly Val Arg Arg Lys Thr Thr 195 200 205 gca agg agg acg gcc ggt aag agt acg gtg cgc agg aag agc aca gtc 672 Ala Arg Arg Thr Ala Gly Lys Ser Thr Val Arg Arg Lys Ser Thr Val 210 215 220 aag agg acg gtg cac agg aag acc ggc aag aag gca gta gta cgc agg 720 Lys Arg Thr Val His Arg Lys Thr Gly Lys Lys Ala Val Val Arg Arg 225 230 235 240 aag agc aca gtc aag agg acg gca cgg agg ccg gcc ggc aga aag acc 768 Lys Ser Thr Val Lys Arg Thr Ala Arg Arg Pro Ala Gly Arg Lys Thr 245 250 255 ccc gga agg gcc gcg cgc agg gcc ggc gca aag agg cgc tag 810 Pro Gly Arg Ala Ala Arg Arg Ala Gly Ala Lys Arg Arg 260 265 cctgctgat 819 <210> SEQ ID NO 6 <211> LENGTH: 269 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 6 Met His Gly Ile Glu Gly Gly Arg Gly Asp Met Ser Glu Asn Phe Val 1 5 10 15 Ala Phe Cys Val Ala Cys Ala Arg Gly Val Thr Lys Asp Glu Met Lys 20 25 30 Tyr Val Asp Gly Arg Val Phe His Lys Glu Cys His Ala Arg His Gly 35 40 45 Gly Gln Ile Arg Phe Pro Asn Pro Glu Val Glu Gln Arg Val Ala Glu 50 55 60 Leu Lys Val Asp Leu Ile Gln Met Arg Asn Gln Leu Ala Glu Met Asn 65 70 75 80 Arg Ala Ser Gly Asp Gly Gly Val His Ser Ser Ala Thr Ser Ala Ala 85 90 95 Glu Ala Glu Gln His Arg Ala Glu Leu Lys Val Gln Leu Val Gln Met 100 105 110 Arg Asn Gln Leu Ala Glu Met Asn Arg Lys Ala Pro Gly Lys Pro Ala 115 120 125 Arg Lys Lys Ala Ala Gly Lys Thr Ala Arg Arg Lys Ser Gly Lys Lys 130 135 140 Thr Val Arg Arg Lys Thr Gly Lys Arg Thr Ala Gly Lys Lys Ala Gly 145 150 155 160 Ala Arg Arg Lys Thr Thr Val Lys Arg Thr Ala Arg Arg Lys Thr Thr 165 170 175 Ala Lys Lys Ala Ala Gly Arg Lys Ala Gly Ala Arg Arg Lys Ala Thr 180 185 190 Val Lys Arg Thr Val His Lys Lys Ile Gly Val Arg Arg Lys Thr Thr 195 200 205 Ala Arg Arg Thr Ala Gly Lys Ser Thr Val Arg Arg Lys Ser Thr Val 210 215 220 Lys Arg Thr Val His Arg Lys Thr Gly Lys Lys Ala Val Val Arg Arg 225 230 235 240 Lys Ser Thr Val Lys Arg Thr Ala Arg Arg Pro Ala Gly Arg Lys Thr 245 250 255 Pro Gly Arg Ala Ala Arg Arg Ala Gly Ala Lys Arg Arg 260 265 <210> SEQ ID NO 7 <211> LENGTH: 1569 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1569) <400> SEQUENCE: 7 atg cag tcg ctt gga cgg cta gac gag gcg tgc gcg gag ata tcg cgc 48 Met Gln Ser Leu Gly Arg Leu Asp Glu Ala Cys Ala Glu Ile Ser Arg 1 5 10 15 agc ctg ctt gaa tac gag tcc ccc acc gcc ggt gat gtc cgg acg gag 96 Ser Leu Leu Glu Tyr Glu Ser Pro Thr Ala Gly Asp Val Arg Thr Glu 20 25 30 atc aga agg gca tgc aca aag tac tcg ctc cgg agg atc cca aag aac 144 Ile Arg Arg Ala Cys Thr Lys Tyr Ser Leu Arg Arg Ile Pro Lys Asn 35 40 45 cgc gag ata ctg gcc acc gcc agg ggt cag gac ttt gac agg ctg cgc 192 Arg Glu Ile Leu Ala Thr Ala Arg Gly Gln Asp Phe Asp Arg Leu Arg 50 55 60 ccc ctg ctg ctc aaa aag ccc gta aag acc gca tcc ggg gtg gcc gtg 240 Pro Leu Leu Leu Lys Lys Pro Val Lys Thr Ala Ser Gly Val Ala Val 65 70 75 80 ata gca gtc atg ccc atg ccg tac gcg tgc ccc cac ggc aga tgc aca 288 Ile Ala Val Met Pro Met Pro Tyr Ala Cys Pro His Gly Arg Cys Thr 85 90 95 tac tgc ccc ggc ggg gag gcg tcg aac aca ccc aac agc tat acc ggc 336 Tyr Cys Pro Gly Gly Glu Ala Ser Asn Thr Pro Asn Ser Tyr Thr Gly 100 105 110 ggc gag ccc ata gcg gcg ggc gcc atg aac agc ggg tac gac ccg gaa 384 Gly Glu Pro Ile Ala Ala Gly Ala Met Asn Ser Gly Tyr Asp Pro Glu 115 120 125 gag cag gtc cgc gcg ggt ctg gcc cgg ctg cgc gcg cac ggc cac gat 432 Glu Gln Val Arg Ala Gly Leu Ala Arg Leu Arg Ala His Gly His Asp 130 135 140 gta gcc aag ctg gag ata gta ata gtg ggc ggc aca ttc ctg ttc atg 480 Val Ala Lys Leu Glu Ile Val Ile Val Gly Gly Thr Phe Leu Phe Met 145 150 155 160 ccg cag gag tac cag gag tgg ttc gtc aag tcc tgt tat gac gcg ctc 528 Pro Gln Glu Tyr Gln Glu Trp Phe Val Lys Ser Cys Tyr Asp Ala Leu 165 170 175 aac ggg tcc gct tcc gcg ggg atg gag gag gcc aag cac cga aat gaa 576 Asn Gly Ser Ala Ser Ala Gly Met Glu Glu Ala Lys His Arg Asn Glu 180 185 190 act gcc gtg cac aga aac gtg ggc ctc acc ata gag acc aag ccg gac 624 Thr Ala Val His Arg Asn Val Gly Leu Thr Ile Glu Thr Lys Pro Asp 195 200 205 tat tgc agg aca gag cat gtg gac gcg atg ctc ggc ttt ggg gcc acg 672 Tyr Cys Arg Thr Glu His Val Asp Ala Met Leu Gly Phe Gly Ala Thr 210 215 220 cgc gtg gag ata ggc gtg cag agc ctc cgg gag gag gtc tac ttg agg 720 Arg Val Glu Ile Gly Val Gln Ser Leu Arg Glu Glu Val Tyr Leu Arg 225 230 235 240 gtc aac cgg ggg cac ggc tac cag gat gtg aca gag tcg ttt gcc gcc 768 Val Asn Arg Gly His Gly Tyr Gln Asp Val Thr Glu Ser Phe Ala Ala 245 250 255 gcc agg gat gca ggc tac aag gtg gct gcc cac atg atg cca gga ctc 816 Ala Arg Asp Ala Gly Tyr Lys Val Ala Ala His Met Met Pro Gly Leu 260 265 270 ccg ggg gcc acc ccg gaa ggc gac atc gag gat ctg cgc atg ctg ttt 864 Pro Gly Ala Thr Pro Glu Gly Asp Ile Glu Asp Leu Arg Met Leu Phe 275 280 285 gag gat ccc gcg ctc agg ccg gac atg ctc aag gtg tac ccc gcg cta 912 Glu Asp Pro Ala Leu Arg Pro Asp Met Leu Lys Val Tyr Pro Ala Leu 290 295 300 gta gta agg ggc acc ccc atg tat gag gag tat tcg agg ggc gag tat 960 Val Val Arg Gly Thr Pro Met Tyr Glu Glu Tyr Ser Arg Gly Glu Tyr 305 310 315 320 tcc ccg tat acg gaa gag gag gtc atc cgg gtg ctc tcc gag gcc aag 1008 Ser Pro Tyr Thr Glu Glu Glu Val Ile Arg Val Leu Ser Glu Ala Lys 325 330 335 gcg cgc gtg ccc agg tgg gcg agg ata atg cgc gtg cag cgc gag ata 1056 Ala Arg Val Pro Arg Trp Ala Arg Ile Met Arg Val Gln Arg Glu Ile 340 345 350 cac ccc gac gag ata gtg gcc ggg ccg agg agc ggc aac ctc cgc cag 1104 His Pro Asp Glu Ile Val Ala Gly Pro Arg Ser Gly Asn Leu Arg Gln 355 360 365 ctg gtg cac aag agg ctc caa gag cag ggc cgc cga tgc cgc tgc ata 1152 Leu Val His Lys Arg Leu Gln Glu Gln Gly Arg Arg Cys Arg Cys Ile 370 375 380 cgg tgc agg gag gcg ggg ctc gcg ggg agg acc gtg ccg cag aag ctc 1200 Arg Cys Arg Glu Ala Gly Leu Ala Gly Arg Thr Val Pro Gln Lys Leu 385 390 395 400 cgt att gac agg gcg gac tat tcg gcc tcg ggg ggg aga gaa tcg ttt 1248 Arg Ile Asp Arg Ala Asp Tyr Ser Ala Ser Gly Gly Arg Glu Ser Phe 405 410 415 atc tcg ctt gta gac ggg gat gat gcc atc tat ggc ttt gtg cgc ctg 1296 Ile Ser Leu Val Asp Gly Asp Asp Ala Ile Tyr Gly Phe Val Arg Leu 420 425 430 cgc aag ccc tcc gga gca gca cac agg ccg gag gtc aca ccg gaa tcc 1344 Arg Lys Pro Ser Gly Ala Ala His Arg Pro Glu Val Thr Pro Glu Ser 435 440 445 tgc ata ata cgc gag ctg cac gta tac ggc agg tcg ctt ggc ctc ggc 1392 Cys Ile Ile Arg Glu Leu His Val Tyr Gly Arg Ser Leu Gly Leu Gly 450 455 460 gag agg ggc ggc ata cag cac tcg ggt cta ggc aga agg ctc gtc tca 1440 Glu Arg Gly Gly Ile Gln His Ser Gly Leu Gly Arg Arg Leu Val Ser 465 470 475 480 gaa gca gag tct gcc gcc cgt gag ctt ggc gcg ggc agg ctc ctt gtg 1488 Glu Ala Glu Ser Ala Ala Arg Glu Leu Gly Ala Gly Arg Leu Leu Val 485 490 495 ata agc gcc gtc ggg aca agg ggt tac tat cgc agg ctc gga tat tca 1536 Ile Ser Ala Val Gly Thr Arg Gly Tyr Tyr Arg Arg Leu Gly Tyr Ser 500 505 510 cgc acg ggc ccc tac atg ggg aag gtg ctc tga 1569 Arg Thr Gly Pro Tyr Met Gly Lys Val Leu 515 520 <210> SEQ ID NO 8 <211> LENGTH: 522 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 8 Met Gln Ser Leu Gly Arg Leu Asp Glu Ala Cys Ala Glu Ile Ser Arg 1 5 10 15 Ser Leu Leu Glu Tyr Glu Ser Pro Thr Ala Gly Asp Val Arg Thr Glu 20 25 30 Ile Arg Arg Ala Cys Thr Lys Tyr Ser Leu Arg Arg Ile Pro Lys Asn 35 40 45 Arg Glu Ile Leu Ala Thr Ala Arg Gly Gln Asp Phe Asp Arg Leu Arg 50 55 60 Pro Leu Leu Leu Lys Lys Pro Val Lys Thr Ala Ser Gly Val Ala Val 65 70 75 80 Ile Ala Val Met Pro Met Pro Tyr Ala Cys Pro His Gly Arg Cys Thr 85 90 95 Tyr Cys Pro Gly Gly Glu Ala Ser Asn Thr Pro Asn Ser Tyr Thr Gly 100 105 110 Gly Glu Pro Ile Ala Ala Gly Ala Met Asn Ser Gly Tyr Asp Pro Glu 115 120 125 Glu Gln Val Arg Ala Gly Leu Ala Arg Leu Arg Ala His Gly His Asp 130 135 140 Val Ala Lys Leu Glu Ile Val Ile Val Gly Gly Thr Phe Leu Phe Met 145 150 155 160 Pro Gln Glu Tyr Gln Glu Trp Phe Val Lys Ser Cys Tyr Asp Ala Leu 165 170 175 Asn Gly Ser Ala Ser Ala Gly Met Glu Glu Ala Lys His Arg Asn Glu 180 185 190 Thr Ala Val His Arg Asn Val Gly Leu Thr Ile Glu Thr Lys Pro Asp 195 200 205 Tyr Cys Arg Thr Glu His Val Asp Ala Met Leu Gly Phe Gly Ala Thr 210 215 220 Arg Val Glu Ile Gly Val Gln Ser Leu Arg Glu Glu Val Tyr Leu Arg 225 230 235 240 Val Asn Arg Gly His Gly Tyr Gln Asp Val Thr Glu Ser Phe Ala Ala 245 250 255 Ala Arg Asp Ala Gly Tyr Lys Val Ala Ala His Met Met Pro Gly Leu 260 265 270 Pro Gly Ala Thr Pro Glu Gly Asp Ile Glu Asp Leu Arg Met Leu Phe 275 280 285 Glu Asp Pro Ala Leu Arg Pro Asp Met Leu Lys Val Tyr Pro Ala Leu 290 295 300 Val Val Arg Gly Thr Pro Met Tyr Glu Glu Tyr Ser Arg Gly Glu Tyr 305 310 315 320 Ser Pro Tyr Thr Glu Glu Glu Val Ile Arg Val Leu Ser Glu Ala Lys 325 330 335 Ala Arg Val Pro Arg Trp Ala Arg Ile Met Arg Val Gln Arg Glu Ile 340 345 350 His Pro Asp Glu Ile Val Ala Gly Pro Arg Ser Gly Asn Leu Arg Gln 355 360 365 Leu Val His Lys Arg Leu Gln Glu Gln Gly Arg Arg Cys Arg Cys Ile 370 375 380 Arg Cys Arg Glu Ala Gly Leu Ala Gly Arg Thr Val Pro Gln Lys Leu 385 390 395 400 Arg Ile Asp Arg Ala Asp Tyr Ser Ala Ser Gly Gly Arg Glu Ser Phe 405 410 415 Ile Ser Leu Val Asp Gly Asp Asp Ala Ile Tyr Gly Phe Val Arg Leu 420 425 430 Arg Lys Pro Ser Gly Ala Ala His Arg Pro Glu Val Thr Pro Glu Ser 435 440 445 Cys Ile Ile Arg Glu Leu His Val Tyr Gly Arg Ser Leu Gly Leu Gly 450 455 460 Glu Arg Gly Gly Ile Gln His Ser Gly Leu Gly Arg Arg Leu Val Ser 465 470 475 480 Glu Ala Glu Ser Ala Ala Arg Glu Leu Gly Ala Gly Arg Leu Leu Val 485 490 495 Ile Ser Ala Val Gly Thr Arg Gly Tyr Tyr Arg Arg Leu Gly Tyr Ser 500 505 510 Arg Thr Gly Pro Tyr Met Gly Lys Val Leu 515 520 <210> SEQ ID NO 9 <211> LENGTH: 1575 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1575) <400> SEQUENCE: 9 atg gag acg ata ggc cgc ggc acc tgg ata gac aag ctg gcg cat gaa 48 Met Glu Thr Ile Gly Arg Gly Thr Trp Ile Asp Lys Leu Ala His Glu 1 5 10 15 ctg gta gag cgc gaa gag gcc ctc ggc cgg gat aca gag atg ata aac 96 Leu Val Glu Arg Glu Glu Ala Leu Gly Arg Asp Thr Glu Met Ile Asn 20 25 30 gtc gag agc ggc ctt ggc gcg tcc ggg ata ccc cac atg ggg agc ctc 144 Val Glu Ser Gly Leu Gly Ala Ser Gly Ile Pro His Met Gly Ser Leu 35 40 45 ggg gat gca gtc agg gcg tac ggc gtg ggg ctc gcc gtc ggc gac atg 192 Gly Asp Ala Val Arg Ala Tyr Gly Val Gly Leu Ala Val Gly Asp Met 50 55 60 ggg cac agc ttc cgg ctc ata gcg tac ttt gac gac ctc gac ggg ctc 240 Gly His Ser Phe Arg Leu Ile Ala Tyr Phe Asp Asp Leu Asp Gly Leu 65 70 75 80 cgc aag gtc ccc gag ggc atg cca tcc tcg cta gaa gag cac ata gcc 288 Arg Lys Val Pro Glu Gly Met Pro Ser Ser Leu Glu Glu His Ile Ala 85 90 95 cgt ccc gtc tcg gcg ata ccc gac ccc tac ggg tgc cac gat tcc tac 336 Arg Pro Val Ser Ala Ile Pro Asp Pro Tyr Gly Cys His Asp Ser Tyr 100 105 110 ggc atg cac atg agc ggc ctg ctg cta gag ggg ctc gac gca ctg ggc 384 Gly Met His Met Ser Gly Leu Leu Leu Glu Gly Leu Asp Ala Leu Gly 115 120 125 ata gag tat gac ttt agg cgg gca agg gac acg tac cgc gac ggc ctg 432 Ile Glu Tyr Asp Phe Arg Arg Ala Arg Asp Thr Tyr Arg Asp Gly Leu 130 135 140 ctc gca gaa cag atc cac agg ata cta tcg aac agc tcg gta ata ggg 480 Leu Ala Glu Gln Ile His Arg Ile Leu Ser Asn Ser Ser Val Ile Gly 145 150 155 160 gag aag ata gcc gag atg gtg ggc cag gaa aag ttt cgc agc agc ctg 528 Glu Lys Ile Ala Glu Met Val Gly Gln Glu Lys Phe Arg Ser Ser Leu 165 170 175 ccg tac ttt gca gtc tgt gaa cag tgc ggg aag atg tac acg gcc gag 576 Pro Tyr Phe Ala Val Cys Glu Gln Cys Gly Lys Met Tyr Thr Ala Glu 180 185 190 tcc gtt gaa tac ctg gca gac agc cgc aag gtg cgg tac agg tgc ggc 624 Ser Val Glu Tyr Leu Ala Asp Ser Arg Lys Val Arg Tyr Arg Cys Gly 195 200 205 gac gcc gag gta ggc gga aga aag atc gcc ggc tgc ggg cac gag ggc 672 Asp Ala Glu Val Gly Gly Arg Lys Ile Ala Gly Cys Gly His Glu Gly 210 215 220 gag gcg gac acg ggc gga gcc ggc ggc aag ctc gcc tgg aag gtg gag 720 Glu Ala Asp Thr Gly Gly Ala Gly Gly Lys Leu Ala Trp Lys Val Glu 225 230 235 240 ttt gcc gca agg tgg cag gcg ttt gat gta cgc ttt gag gca tac ggc 768 Phe Ala Ala Arg Trp Gln Ala Phe Asp Val Arg Phe Glu Ala Tyr Gly 245 250 255 aag gac atc atg gac tct gta agg ata aac gac tgg gtc tcc gac gag 816 Lys Asp Ile Met Asp Ser Val Arg Ile Asn Asp Trp Val Ser Asp Glu 260 265 270 ata cta tcc agc ccg cac ccc cac cat aca agg tac gag atg ttc ctc 864 Ile Leu Ser Ser Pro His Pro His His Thr Arg Tyr Glu Met Phe Leu 275 280 285 gac aag ggc ggc aaa aag ata tca aag tcg tca gga aac gtg gtc acg 912 Asp Lys Gly Gly Lys Lys Ile Ser Lys Ser Ser Gly Asn Val Val Thr 290 295 300 ccg cag aaa tgg ctc agg tac ggc acc ccc cag tcg ata ctg ctc ctc 960 Pro Gln Lys Trp Leu Arg Tyr Gly Thr Pro Gln Ser Ile Leu Leu Leu 305 310 315 320 atg tac aag cgc atc acg ggg gcg cgg gag ctt ggc ctc gag gat gtg 1008 Met Tyr Lys Arg Ile Thr Gly Ala Arg Glu Leu Gly Leu Glu Asp Val 325 330 335 cca tcc ctg atg gac gag tac ggc gat ctt cag cgc gag tac ttt gcg 1056 Pro Ser Leu Met Asp Glu Tyr Gly Asp Leu Gln Arg Glu Tyr Phe Ala 340 345 350 gga ggg ggc agg ggc ggg aaa gcc cgc gag gcc aag aac agg ggg cta 1104 Gly Gly Gly Arg Gly Gly Lys Ala Arg Glu Ala Lys Asn Arg Gly Leu 355 360 365 ttc gag tat acg aac ctg ctg gag gca cag gag ggg ccg cgg ccg cat 1152 Phe Glu Tyr Thr Asn Leu Leu Glu Ala Gln Glu Gly Pro Arg Pro His 370 375 380 gcg ggc tac cgg ctg cta gtc gag ctc tcc agg ctg ttc agg gag aat 1200 Ala Gly Tyr Arg Leu Leu Val Glu Leu Ser Arg Leu Phe Arg Glu Asn 385 390 395 400 agg acc gag cgc gtc aca aaa aag ctc gtc gag tac ggg gta att gac 1248 Arg Thr Glu Arg Val Thr Lys Lys Leu Val Glu Tyr Gly Val Ile Asp 405 410 415 ggg ccc tcg ccc ggg atc gag cgg ctc ata gca ctg gcc gga aac tat 1296 Gly Pro Ser Pro Gly Ile Glu Arg Leu Ile Ala Leu Ala Gly Asn Tyr 420 425 430 gca gac gac atg tat tct gcc gag aga aca gag gtg gag ctt gac ggg 1344 Ala Asp Asp Met Tyr Ser Ala Glu Arg Thr Glu Val Glu Leu Asp Gly 435 440 445 gcc aca agg ggg gcc ctc tcg gag ctg gca gaa atg ctc ggt tcc gcc 1392 Ala Thr Arg Gly Ala Leu Ser Glu Leu Ala Glu Met Leu Gly Ser Ala 450 455 460 ccg gag ggc gga ctg cag gat gtc ata tac ggc gtg gcc aag tcc cac 1440 Pro Glu Gly Gly Leu Gln Asp Val Ile Tyr Gly Val Ala Lys Ser His 465 470 475 480 ggg gtg ccc ccg cgc gac ttt ttc aag gcg ctg tac agg ata ata ctg 1488 Gly Val Pro Pro Arg Asp Phe Phe Lys Ala Leu Tyr Arg Ile Ile Leu 485 490 495 gat gca tcc agc ggg ccg agg ata ggc ccc ttc ata gag gac ata ggc 1536 Asp Ala Ser Ser Gly Pro Arg Ile Gly Pro Phe Ile Glu Asp Ile Gly 500 505 510 agg gag aag gtg gca ggt atg ata cgg ggg cgc ctc tga 1575 Arg Glu Lys Val Ala Gly Met Ile Arg Gly Arg Leu 515 520 <210> SEQ ID NO 10 <211> LENGTH: 524 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 10 Met Glu Thr Ile Gly Arg Gly Thr Trp Ile Asp Lys Leu Ala His Glu 1 5 10 15 Leu Val Glu Arg Glu Glu Ala Leu Gly Arg Asp Thr Glu Met Ile Asn 20 25 30 Val Glu Ser Gly Leu Gly Ala Ser Gly Ile Pro His Met Gly Ser Leu 35 40 45 Gly Asp Ala Val Arg Ala Tyr Gly Val Gly Leu Ala Val Gly Asp Met 50 55 60 Gly His Ser Phe Arg Leu Ile Ala Tyr Phe Asp Asp Leu Asp Gly Leu 65 70 75 80 Arg Lys Val Pro Glu Gly Met Pro Ser Ser Leu Glu Glu His Ile Ala 85 90 95 Arg Pro Val Ser Ala Ile Pro Asp Pro Tyr Gly Cys His Asp Ser Tyr 100 105 110 Gly Met His Met Ser Gly Leu Leu Leu Glu Gly Leu Asp Ala Leu Gly 115 120 125 Ile Glu Tyr Asp Phe Arg Arg Ala Arg Asp Thr Tyr Arg Asp Gly Leu 130 135 140 Leu Ala Glu Gln Ile His Arg Ile Leu Ser Asn Ser Ser Val Ile Gly 145 150 155 160 Glu Lys Ile Ala Glu Met Val Gly Gln Glu Lys Phe Arg Ser Ser Leu 165 170 175 Pro Tyr Phe Ala Val Cys Glu Gln Cys Gly Lys Met Tyr Thr Ala Glu 180 185 190 Ser Val Glu Tyr Leu Ala Asp Ser Arg Lys Val Arg Tyr Arg Cys Gly 195 200 205 Asp Ala Glu Val Gly Gly Arg Lys Ile Ala Gly Cys Gly His Glu Gly 210 215 220 Glu Ala Asp Thr Gly Gly Ala Gly Gly Lys Leu Ala Trp Lys Val Glu 225 230 235 240 Phe Ala Ala Arg Trp Gln Ala Phe Asp Val Arg Phe Glu Ala Tyr Gly 245 250 255 Lys Asp Ile Met Asp Ser Val Arg Ile Asn Asp Trp Val Ser Asp Glu 260 265 270 Ile Leu Ser Ser Pro His Pro His His Thr Arg Tyr Glu Met Phe Leu 275 280 285 Asp Lys Gly Gly Lys Lys Ile Ser Lys Ser Ser Gly Asn Val Val Thr 290 295 300 Pro Gln Lys Trp Leu Arg Tyr Gly Thr Pro Gln Ser Ile Leu Leu Leu 305 310 315 320 Met Tyr Lys Arg Ile Thr Gly Ala Arg Glu Leu Gly Leu Glu Asp Val 325 330 335 Pro Ser Leu Met Asp Glu Tyr Gly Asp Leu Gln Arg Glu Tyr Phe Ala 340 345 350 Gly Gly Gly Arg Gly Gly Lys Ala Arg Glu Ala Lys Asn Arg Gly Leu 355 360 365 Phe Glu Tyr Thr Asn Leu Leu Glu Ala Gln Glu Gly Pro Arg Pro His 370 375 380 Ala Gly Tyr Arg Leu Leu Val Glu Leu Ser Arg Leu Phe Arg Glu Asn 385 390 395 400 Arg Thr Glu Arg Val Thr Lys Lys Leu Val Glu Tyr Gly Val Ile Asp 405 410 415 Gly Pro Ser Pro Gly Ile Glu Arg Leu Ile Ala Leu Ala Gly Asn Tyr 420 425 430 Ala Asp Asp Met Tyr Ser Ala Glu Arg Thr Glu Val Glu Leu Asp Gly 435 440 445 Ala Thr Arg Gly Ala Leu Ser Glu Leu Ala Glu Met Leu Gly Ser Ala 450 455 460 Pro Glu Gly Gly Leu Gln Asp Val Ile Tyr Gly Val Ala Lys Ser His 465 470 475 480 Gly Val Pro Pro Arg Asp Phe Phe Lys Ala Leu Tyr Arg Ile Ile Leu 485 490 495 Asp Ala Ser Ser Gly Pro Arg Ile Gly Pro Phe Ile Glu Asp Ile Gly 500 505 510 Arg Glu Lys Val Ala Gly Met Ile Arg Gly Arg Leu 515 520 <210> SEQ ID NO 11 <211> LENGTH: 885 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum sybiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(885) <400> SEQUENCE: 11 atg gag tca gcc ggt gag cag gca cct ggt gtg gta ctt cac gac tat 48 Met Glu Ser Ala Gly Glu Gln Ala Pro Gly Val Val Leu His Asp Tyr 1 5 10 15 ctt tca aaa ttg caa cag tat tcg ggg agg gac aca att cta tat gcg 96 Leu Ser Lys Leu Gln Gln Tyr Ser Gly Arg Asp Thr Ile Leu Tyr Ala 20 25 30 acc aac tgg atg acg gac gaa ccg cat acg cct aat gaa gct ctc ata 144 Thr Asn Trp Met Thr Asp Glu Pro His Thr Pro Asn Glu Ala Leu Ile 35 40 45 aca aat ggt gac ctg tat gga ttt atg agg atg atg cgt gat tta aag 192 Thr Asn Gly Asp Leu Tyr Gly Phe Met Arg Met Met Arg Asp Leu Lys 50 55 60 act aaa aaa ttg gat ctg ata ctc cac agt cct gga ggt tct gcc gag 240 Thr Lys Lys Leu Asp Leu Ile Leu His Ser Pro Gly Gly Ser Ala Glu 65 70 75 80 tct gca gaa tcg att gtc aca tac ctt cat gcg aaa tat gat gat att 288 Ser Ala Glu Ser Ile Val Thr Tyr Leu His Ala Lys Tyr Asp Asp Ile 85 90 95 cgg gtc atc ata ccg tat gcc gca atg tca gca gcc tcg atg ctt gct 336 Arg Val Ile Ile Pro Tyr Ala Ala Met Ser Ala Ala Ser Met Leu Ala 100 105 110 tgc gca tca aat tcc ctg gta atg ggc aaa cac tcg tct ata gga ccc 384 Cys Ala Ser Asn Ser Leu Val Met Gly Lys His Ser Ser Ile Gly Pro 115 120 125 gct gat ccc caa ttt att ttc cca acc aag att ggc atg caa ata atg 432 Ala Asp Pro Gln Phe Ile Phe Pro Thr Lys Ile Gly Met Gln Ile Met 130 135 140 tct gca cag ctt cta att gac gag ttg caa gaa gtg cag gtg gta tct 480 Ser Ala Gln Leu Leu Ile Asp Glu Leu Gln Glu Val Gln Val Val Ser 145 150 155 160 gaa aaa cat ccg ggc agg ctt ggc gca tgg ctt cca ttg tta gga caa 528 Glu Lys His Pro Gly Arg Leu Gly Ala Trp Leu Pro Leu Leu Gly Gln 165 170 175 tat cct cct gga ctg gtt caa aaa tgc att agc agc cag aaa cta gct 576 Tyr Pro Pro Gly Leu Val Gln Lys Cys Ile Ser Ser Gln Lys Leu Ala 180 185 190 gaa gtg ctt gta caa aaa tgg ctg gaa gac cac atg ttt gct ggc gag 624 Glu Val Leu Val Gln Lys Trp Leu Glu Asp His Met Phe Ala Gly Glu 195 200 205 tct gat gcg gca gaa aaa tca aaa aaa ata tct gga atg tta gct tct 672 Ser Asp Ala Ala Glu Lys Ser Lys Lys Ile Ser Gly Met Leu Ala Ser 210 215 220 cct gga aaa tat tac agt cat ggg aga tac ata tcg cga gag gag tgt 720 Pro Gly Lys Tyr Tyr Ser His Gly Arg Tyr Ile Ser Arg Glu Glu Cys 225 230 235 240 agg ggc atc ggt ttg aaa ata act gat cta gaa gcc gac caa gaa ttt 768 Arg Gly Ile Gly Leu Lys Ile Thr Asp Leu Glu Ala Asp Gln Glu Phe 245 250 255 cag gat ctg aca ttg tcg gta tct cat gca gcg gat atc ctg tct caa 816 Gln Asp Leu Thr Leu Ser Val Ser His Ala Ala Asp Ile Leu Ser Gln 260 265 270 ttt act cca atc aac aaa atc atc gcg aat cac ctc ggt aat tca gtt 864 Phe Thr Pro Ile Asn Lys Ile Ile Ala Asn His Leu Gly Asn Ser Val 275 280 285 atc agc aaa cca tca aca tag 885 Ile Ser Lys Pro Ser Thr 290 <210> SEQ ID NO 12 <211> LENGTH: 294 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum sybiosum <400> SEQUENCE: 12 Met Glu Ser Ala Gly Glu Gln Ala Pro Gly Val Val Leu His Asp Tyr 1 5 10 15 Leu Ser Lys Leu Gln Gln Tyr Ser Gly Arg Asp Thr Ile Leu Tyr Ala 20 25 30 Thr Asn Trp Met Thr Asp Glu Pro His Thr Pro Asn Glu Ala Leu Ile 35 40 45 Thr Asn Gly Asp Leu Tyr Gly Phe Met Arg Met Met Arg Asp Leu Lys 50 55 60 Thr Lys Lys Leu Asp Leu Ile Leu His Ser Pro Gly Gly Ser Ala Glu 65 70 75 80 Ser Ala Glu Ser Ile Val Thr Tyr Leu His Ala Lys Tyr Asp Asp Ile 85 90 95 Arg Val Ile Ile Pro Tyr Ala Ala Met Ser Ala Ala Ser Met Leu Ala 100 105 110 Cys Ala Ser Asn Ser Leu Val Met Gly Lys His Ser Ser Ile Gly Pro 115 120 125 Ala Asp Pro Gln Phe Ile Phe Pro Thr Lys Ile Gly Met Gln Ile Met 130 135 140 Ser Ala Gln Leu Leu Ile Asp Glu Leu Gln Glu Val Gln Val Val Ser 145 150 155 160 Glu Lys His Pro Gly Arg Leu Gly Ala Trp Leu Pro Leu Leu Gly Gln 165 170 175 Tyr Pro Pro Gly Leu Val Gln Lys Cys Ile Ser Ser Gln Lys Leu Ala 180 185 190 Glu Val Leu Val Gln Lys Trp Leu Glu Asp His Met Phe Ala Gly Glu 195 200 205 Ser Asp Ala Ala Glu Lys Ser Lys Lys Ile Ser Gly Met Leu Ala Ser 210 215 220 Pro Gly Lys Tyr Tyr Ser His Gly Arg Tyr Ile Ser Arg Glu Glu Cys 225 230 235 240 Arg Gly Ile Gly Leu Lys Ile Thr Asp Leu Glu Ala Asp Gln Glu Phe 245 250 255 Gln Asp Leu Thr Leu Ser Val Ser His Ala Ala Asp Ile Leu Ser Gln 260 265 270 Phe Thr Pro Ile Asn Lys Ile Ile Ala Asn His Leu Gly Asn Ser Val 275 280 285 Ile Ser Lys Pro Ser Thr 290 <210> SEQ ID NO 13 <211> LENGTH: 1305 <212> TYPE: DNA <213> ORGANISM: Cenarchaem symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1305) <400> SEQUENCE: 13 gtg gat cta gag cgc gag tac agg gca aag acc agg ggc tcg gcg ggg 48 Met Asp Leu Glu Arg Glu Tyr Arg Ala Lys Thr Arg Gly Ser Ala Gly 1 5 10 15 ata ttt gcc cgg tcg aga agg tac cat gta ggg ggg gtc agc cac aac 96 Ile Phe Ala Arg Ser Arg Arg Tyr His Val Gly Gly Val Ser His Asn 20 25 30 ata agg tac tat gag ccg tac ccg ttt gtt aca agg tcg gcg cgc ggc 144 Ile Arg Tyr Tyr Glu Pro Tyr Pro Phe Val Thr Arg Ser Ala Arg Gly 35 40 45 aag cac ctt gtg gac gtc gac ggg aac aag tat acc gac tat tgg atg 192 Lys His Leu Val Asp Val Asp Gly Asn Lys Tyr Thr Asp Tyr Trp Met 50 55 60 ggg cac tgg agc ctg ata ctc ggc cac gcg ccg gcg caa gta agg tcg 240 Gly His Trp Ser Leu Ile Leu Gly His Ala Pro Ala Gln Val Arg Ser 65 70 75 80 gca gtg gag ggg cag ctg cgc cgc ggc tgg ata cac ggg acc gca aac 288 Ala Val Glu Gly Gln Leu Arg Arg Gly Trp Ile His Gly Thr Ala Asn 85 90 95 gag ccc acc atg cgg ctc tcg gag atc ata cgc ggg gcg gta aag gcg 336 Glu Pro Thr Met Arg Leu Ser Glu Ile Ile Arg Gly Ala Val Lys Ala 100 105 110 gca gag aag ata agg tat gtt aca tcc ggc acg gag gcc gtc atg tat 384 Ala Glu Lys Ile Arg Tyr Val Thr Ser Gly Thr Glu Ala Val Met Tyr 115 120 125 gcg gca agg atg gcg cgc gca cgc acg gga aaa aaa gtg ata gca aag 432 Ala Ala Arg Met Ala Arg Ala Arg Thr Gly Lys Lys Val Ile Ala Lys 130 135 140 gtc gac ggc ggc tgg cac gga tac gcg tcg ggg ctg cta aag tcg gtc 480 Val Asp Gly Gly Trp His Gly Tyr Ala Ser Gly Leu Leu Lys Ser Val 145 150 155 160 aac tgg ccg tac gat gtg ccc gag agc ggg ggg ctc gtc gac gag gag 528 Asn Trp Pro Tyr Asp Val Pro Glu Ser Gly Gly Leu Val Asp Glu Glu 165 170 175 cac acc gtg tcc atc ccg tac aac aat ctg gag gga tcc ctg gag gcg 576 His Thr Val Ser Ile Pro Tyr Asn Asn Leu Glu Gly Ser Leu Glu Ala 180 185 190 cta agg cgc gca ggg ggc gac ctt gca tgt gtc ata gtc gag ccg atg 624 Leu Arg Arg Ala Gly Gly Asp Leu Ala Cys Val Ile Val Glu Pro Met 195 200 205 ctt ggc ggc ggc ggc tgc ata ccg gca gaa ccg gac tat ctc cgc ggc 672 Leu Gly Gly Gly Gly Cys Ile Pro Ala Glu Pro Asp Tyr Leu Arg Gly 210 215 220 ata cag gag ttt gtg cat tcg aag ggt gca ctg ttc att ctc gac gag 720 Ile Gln Glu Phe Val His Ser Lys Gly Ala Leu Phe Ile Leu Asp Glu 225 230 235 240 ata gtc acg ggg ttc cgg ttc gac ttt ggc tgc gcg tac aag aaa atg 768 Ile Val Thr Gly Phe Arg Phe Asp Phe Gly Cys Ala Tyr Lys Lys Met 245 250 255 ggg ctg gac ccc gac gtg gtg gcg ctg gga aag ata gtc ggg ggc gga 816 Gly Leu Asp Pro Asp Val Val Ala Leu Gly Lys Ile Val Gly Gly Gly 260 265 270 ttc ccc ata ggt gtg gtg tgc ggc aag gac gag gtg atg tgc atc tcc 864 Phe Pro Ile Gly Val Val Cys Gly Lys Asp Glu Val Met Cys Ile Ser 275 280 285 gat acc ggc gcg cat gca aga acc gag agg gcg tac att ggc ggc ggc 912 Asp Thr Gly Ala His Ala Arg Thr Glu Arg Ala Tyr Ile Gly Gly Gly 290 295 300 acc ttt tct gca aac ccc gcg acg atg act gcg ggt gcc gcg gca ctc 960 Thr Phe Ser Ala Asn Pro Ala Thr Met Thr Ala Gly Ala Ala Ala Leu 305 310 315 320 ggt gca ctc agg gag aga agg ggc aca cta tac ccc aga ata aac tcc 1008 Gly Ala Leu Arg Glu Arg Arg Gly Thr Leu Tyr Pro Arg Ile Asn Ser 325 330 335 atg ggg gac gac gca agg gcg cgg ctc tcg agg ata ttc gac ggc agg 1056 Met Gly Asp Asp Ala Arg Ala Arg Leu Ser Arg Ile Phe Asp Gly Arg 340 345 350 gtt gca gtg acc ggc agg ggc tcg ctg ttc atg acg cac ttt aca ccg 1104 Val Ala Val Thr Gly Arg Gly Ser Leu Phe Met Thr His Phe Thr Pro 355 360 365 gat ggg gcc cgc agg ata tcc agc gcg gca gat gct gcc gcc tgc gat 1152 Asp Gly Ala Arg Arg Ile Ser Ser Ala Ala Asp Ala Ala Ala Cys Asp 370 375 380 gtg cat ctg ctg cac agg tac cac ctg gac atg att aca agg gac ggc 1200 Val His Leu Leu His Arg Tyr His Leu Asp Met Ile Thr Arg Asp Gly 385 390 395 400 ata ttc ttt ctg cca ggc aag ctg ggg gcc ata tct gcc gcc cac tca 1248 Ile Phe Phe Leu Pro Gly Lys Leu Gly Ala Ile Ser Ala Ala His Ser 405 410 415 agg gcg gac ctt ggg gcc atg tat tcg gcg tct gag cgc ttt gcg ggg 1296 Arg Ala Asp Leu Gly Ala Met Tyr Ser Ala Ser Glu Arg Phe Ala Gly 420 425 430 gga ctg tga 1305 Gly Leu <210> SEQ ID NO 14 <211> LENGTH: 434 <212> TYPE: PRT <213> ORGANISM: Cenarchaem symbiosum <400> SEQUENCE: 14 Met Asp Leu Glu Arg Glu Tyr Arg Ala Lys Thr Arg Gly Ser Ala Gly 1 5 10 15 Ile Phe Ala Arg Ser Arg Arg Tyr His Val Gly Gly Val Ser His Asn 20 25 30 Ile Arg Tyr Tyr Glu Pro Tyr Pro Phe Val Thr Arg Ser Ala Arg Gly 35 40 45 Lys His Leu Val Asp Val Asp Gly Asn Lys Tyr Thr Asp Tyr Trp Met 50 55 60 Gly His Trp Ser Leu Ile Leu Gly His Ala Pro Ala Gln Val Arg Ser 65 70 75 80 Ala Val Glu Gly Gln Leu Arg Arg Gly Trp Ile His Gly Thr Ala Asn 85 90 95 Glu Pro Thr Met Arg Leu Ser Glu Ile Ile Arg Gly Ala Val Lys Ala 100 105 110 Ala Glu Lys Ile Arg Tyr Val Thr Ser Gly Thr Glu Ala Val Met Tyr 115 120 125 Ala Ala Arg Met Ala Arg Ala Arg Thr Gly Lys Lys Val Ile Ala Lys 130 135 140 Val Asp Gly Gly Trp His Gly Tyr Ala Ser Gly Leu Leu Lys Ser Val 145 150 155 160 Asn Trp Pro Tyr Asp Val Pro Glu Ser Gly Gly Leu Val Asp Glu Glu 165 170 175 His Thr Val Ser Ile Pro Tyr Asn Asn Leu Glu Gly Ser Leu Glu Ala 180 185 190 Leu Arg Arg Ala Gly Gly Asp Leu Ala Cys Val Ile Val Glu Pro Met 195 200 205 Leu Gly Gly Gly Gly Cys Ile Pro Ala Glu Pro Asp Tyr Leu Arg Gly 210 215 220 Ile Gln Glu Phe Val His Ser Lys Gly Ala Leu Phe Ile Leu Asp Glu 225 230 235 240 Ile Val Thr Gly Phe Arg Phe Asp Phe Gly Cys Ala Tyr Lys Lys Met 245 250 255 Gly Leu Asp Pro Asp Val Val Ala Leu Gly Lys Ile Val Gly Gly Gly 260 265 270 Phe Pro Ile Gly Val Val Cys Gly Lys Asp Glu Val Met Cys Ile Ser 275 280 285 Asp Thr Gly Ala His Ala Arg Thr Glu Arg Ala Tyr Ile Gly Gly Gly 290 295 300 Thr Phe Ser Ala Asn Pro Ala Thr Met Thr Ala Gly Ala Ala Ala Leu 305 310 315 320 Gly Ala Leu Arg Glu Arg Arg Gly Thr Leu Tyr Pro Arg Ile Asn Ser 325 330 335 Met Gly Asp Asp Ala Arg Ala Arg Leu Ser Arg Ile Phe Asp Gly Arg 340 345 350 Val Ala Val Thr Gly Arg Gly Ser Leu Phe Met Thr His Phe Thr Pro 355 360 365 Asp Gly Ala Arg Arg Ile Ser Ser Ala Ala Asp Ala Ala Ala Cys Asp 370 375 380 Val His Leu Leu His Arg Tyr His Leu Asp Met Ile Thr Arg Asp Gly 385 390 395 400 Ile Phe Phe Leu Pro Gly Lys Leu Gly Ala Ile Ser Ala Ala His Ser 405 410 415 Arg Ala Asp Leu Gly Ala Met Tyr Ser Ala Ser Glu Arg Phe Ala Gly 420 425 430 Gly Leu <210> SEQ ID NO 15 <211> LENGTH: 816 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(816) <400> SEQUENCE: 15 atg ata ctc ttc ggc aag agc gac ccc tcc gac ctg ctc cgc cag gcc 48 Met Ile Leu Phe Gly Lys Ser Asp Pro Ser Asp Leu Leu Arg Gln Ala 1 5 10 15 gat ctt ttg tgc agt ggg aac aag tac aag gcg gca gtg ggc ctg tac 96 Asp Leu Leu Cys Ser Gly Asn Lys Tyr Lys Ala Ala Val Gly Leu Tyr 20 25 30 agc agg ata ctc aag gac gac ccg cag aac agg atg gtc ctg cag aga 144 Ser Arg Ile Leu Lys Asp Asp Pro Gln Asn Arg Met Val Leu Gln Arg 35 40 45 aag ggc ctc gcc ctc aac agg ata aga agg tac tct gat gcc ata acg 192 Lys Gly Leu Ala Leu Asn Arg Ile Arg Arg Tyr Ser Asp Ala Ile Thr 50 55 60 tgc ttt gat ctg ctg ctc gag ctg gat gat ggc gac gcg cct gca tac 240 Cys Phe Asp Leu Leu Leu Glu Leu Asp Asp Gly Asp Ala Pro Ala Tyr 65 70 75 80 aac aac aag gcc ata gcc cag gcc gag ctg ggc gat acg gca tcc gcc 288 Asn Asn Lys Ala Ile Ala Gln Ala Glu Leu Gly Asp Thr Ala Ser Ala 85 90 95 ctg gag aac tat ggc agg gcc atc gaa gcc agc ccc agg tac gcg ccg 336 Leu Glu Asn Tyr Gly Arg Ala Ile Glu Ala Ser Pro Arg Tyr Ala Pro 100 105 110 gcg tac ttt aac agg gcc gtc ctg ctc gac agg ctc ggc gag cac gaa 384 Ala Tyr Phe Asn Arg Ala Val Leu Leu Asp Arg Leu Gly Glu His Glu 115 120 125 gac gcg ctg ccg gac ctc gac aag gcg aca agg ctg gac agg gac aag 432 Asp Ala Leu Pro Asp Leu Asp Lys Ala Thr Arg Leu Asp Arg Asp Lys 130 135 140 gcc aac ccg agg ttc tac aag ggg ata gtc ctg gga aag atg ggc cgg 480 Ala Asn Pro Arg Phe Tyr Lys Gly Ile Val Leu Gly Lys Met Gly Arg 145 150 155 160 cat gca gag gcg ctg tcc tgc ttc aag gag gtg tgc agg gcg gac cac 528 His Ala Glu Ala Leu Ser Cys Phe Lys Glu Val Cys Arg Ala Asp His 165 170 175 ggc cac gcc gac tca cag ttc cac gtg gcg ata gag gta gcc gag ctc 576 Gly His Ala Asp Ser Gln Phe His Val Ala Ile Glu Val Ala Glu Leu 180 185 190 ggc aaa cac gcc gaa gcc ctc ggt gag ctt gcg gca ctg ccc gca gag 624 Gly Lys His Ala Glu Ala Leu Gly Glu Leu Ala Ala Leu Pro Ala Glu 195 200 205 tac cgc gag aac gca aac gtt ctc tac gcc cgg gcg cgc agc ctc gcc 672 Tyr Arg Glu Asn Ala Asn Val Leu Tyr Ala Arg Ala Arg Ser Leu Ala 210 215 220 ggc ctg gac agg tac gac gag tcc att gca cac ctg caa aag gcc gcc 720 Gly Leu Asp Arg Tyr Asp Glu Ser Ile Ala His Leu Gln Lys Ala Ala 225 230 235 240 aga aag gac tcc aag aca ata aaa aag tgg gcc cgc gcc gag aag gcc 768 Arg Lys Asp Ser Lys Thr Ile Lys Lys Trp Ala Arg Ala Glu Lys Ala 245 250 255 ttt gat cat ata cgg gat gat ccc agg ttc aaa aag ata gcc ggg taa 816 Phe Asp His Ile Arg Asp Asp Pro Arg Phe Lys Lys Ile Ala Gly 260 265 270 <210> SEQ ID NO 16 <211> LENGTH: 271 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 16 Met Ile Leu Phe Gly Lys Ser Asp Pro Ser Asp Leu Leu Arg Gln Ala 1 5 10 15 Asp Leu Leu Cys Ser Gly Asn Lys Tyr Lys Ala Ala Val Gly Leu Tyr 20 25 30 Ser Arg Ile Leu Lys Asp Asp Pro Gln Asn Arg Met Val Leu Gln Arg 35 40 45 Lys Gly Leu Ala Leu Asn Arg Ile Arg Arg Tyr Ser Asp Ala Ile Thr 50 55 60 Cys Phe Asp Leu Leu Leu Glu Leu Asp Asp Gly Asp Ala Pro Ala Tyr 65 70 75 80 Asn Asn Lys Ala Ile Ala Gln Ala Glu Leu Gly Asp Thr Ala Ser Ala 85 90 95 Leu Glu Asn Tyr Gly Arg Ala Ile Glu Ala Ser Pro Arg Tyr Ala Pro 100 105 110 Ala Tyr Phe Asn Arg Ala Val Leu Leu Asp Arg Leu Gly Glu His Glu 115 120 125 Asp Ala Leu Pro Asp Leu Asp Lys Ala Thr Arg Leu Asp Arg Asp Lys 130 135 140 Ala Asn Pro Arg Phe Tyr Lys Gly Ile Val Leu Gly Lys Met Gly Arg 145 150 155 160 His Ala Glu Ala Leu Ser Cys Phe Lys Glu Val Cys Arg Ala Asp His 165 170 175 Gly His Ala Asp Ser Gln Phe His Val Ala Ile Glu Val Ala Glu Leu 180 185 190 Gly Lys His Ala Glu Ala Leu Gly Glu Leu Ala Ala Leu Pro Ala Glu 195 200 205 Tyr Arg Glu Asn Ala Asn Val Leu Tyr Ala Arg Ala Arg Ser Leu Ala 210 215 220 Gly Leu Asp Arg Tyr Asp Glu Ser Ile Ala His Leu Gln Lys Ala Ala 225 230 235 240 Arg Lys Asp Ser Lys Thr Ile Lys Lys Trp Ala Arg Ala Glu Lys Ala 245 250 255 Phe Asp His Ile Arg Asp Asp Pro Arg Phe Lys Lys Ile Ala Gly 260 265 270 <210> SEQ ID NO 17 <211> LENGTH: 696 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(696) <400> SEQUENCE: 17 gtg act gac aag aca agg atc atc gtc ctg cgc aac gcc atg act gaa 48 Met Thr Asp Lys Thr Arg Ile Ile Val Leu Arg Asn Ala Met Thr Glu 1 5 10 15 cag tcc gcc cgg gcc atg atc gag gca aaa aag acg ggg cca ttc agg 96 Gln Ser Ala Arg Ala Met Ile Glu Ala Lys Lys Thr Gly Pro Phe Arg 20 25 30 gcc atg atg agg gcg ccc cca aag gag gac gtc cat gta cat tcc gta 144 Ala Met Met Arg Ala Pro Pro Lys Glu Asp Val His Val His Ser Val 35 40 45 agg ctc gtc cac gag gcg ctc atc cgc gtc tcc gcc cgg tac tcg gcc 192 Arg Leu Val His Glu Ala Leu Ile Arg Val Ser Ala Arg Tyr Ser Ala 50 55 60 gac ttt ttc aga agg gcc gtg cac ccg atc aag gtg gat cag aac gtg 240 Asp Phe Phe Arg Arg Ala Val His Pro Ile Lys Val Asp Gln Asn Val 65 70 75 80 atc gag gtg gtg ctg ggc gac ggc gtc ttc ccg ata agg tca aag tcg 288 Ile Glu Val Val Leu Gly Asp Gly Val Phe Pro Ile Arg Ser Lys Ser 85 90 95 cgc ata cgc aag acc ctg tcc gcc ggg cgc ggc aag aac agg gtc gat 336 Arg Ile Arg Lys Thr Leu Ser Ala Gly Arg Gly Lys Asn Arg Val Asp 100 105 110 ctg gaa ctc gag gag cac gta tac gcg gaa tca gag ggc gtg atg tgc 384 Leu Glu Leu Glu Glu His Val Tyr Ala Glu Ser Glu Gly Val Met Cys 115 120 125 ctt gac cgg cac ggc ggg gag acc ggc ttt ccc tac aag acg ggg acc 432 Leu Asp Arg His Gly Gly Glu Thr Gly Phe Pro Tyr Lys Thr Gly Thr 130 135 140 ggc gcg gtc gag ccg tac ccg cgg cgc atg ctt gat tcg tcg gag aat 480 Gly Ala Val Glu Pro Tyr Pro Arg Arg Met Leu Asp Ser Ser Glu Asn 145 150 155 160 gtg cgg cgc ccg gag ata gac acc ggg gtg gcg ctg gaa aaa ctc cgg 528 Val Arg Arg Pro Glu Ile Asp Thr Gly Val Ala Leu Glu Lys Leu Arg 165 170 175 gta aag ctc cgc ggg ccc ccg cct gac ggc atg cgc gac ctc cgg gag 576 Val Lys Leu Arg Gly Pro Pro Pro Asp Gly Met Arg Asp Leu Arg Glu 180 185 190 gag ttt gca gtc aga tcg gtc gaa gaa gtg tat gcc cct gtc tac gag 624 Glu Phe Ala Val Arg Ser Val Glu Glu Val Tyr Ala Pro Val Tyr Glu 195 200 205 tcg cgg ctt gtg ggg ccc aaa aaa aag gtc cgg ata atg cgg ata gac 672 Ser Arg Leu Val Gly Pro Lys Lys Lys Val Arg Ile Met Arg Ile Asp 210 215 220 gcg gca aga aaa aag atg ctg tag 696 Ala Ala Arg Lys Lys Met Leu 225 230 <210> SEQ ID NO 18 <211> LENGTH: 231 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 18 Met Thr Asp Lys Thr Arg Ile Ile Val Leu Arg Asn Ala Met Thr Glu 1 5 10 15 Gln Ser Ala Arg Ala Met Ile Glu Ala Lys Lys Thr Gly Pro Phe Arg 20 25 30 Ala Met Met Arg Ala Pro Pro Lys Glu Asp Val His Val His Ser Val 35 40 45 Arg Leu Val His Glu Ala Leu Ile Arg Val Ser Ala Arg Tyr Ser Ala 50 55 60 Asp Phe Phe Arg Arg Ala Val His Pro Ile Lys Val Asp Gln Asn Val 65 70 75 80 Ile Glu Val Val Leu Gly Asp Gly Val Phe Pro Ile Arg Ser Lys Ser 85 90 95 Arg Ile Arg Lys Thr Leu Ser Ala Gly Arg Gly Lys Asn Arg Val Asp 100 105 110 Leu Glu Leu Glu Glu His Val Tyr Ala Glu Ser Glu Gly Val Met Cys 115 120 125 Leu Asp Arg His Gly Gly Glu Thr Gly Phe Pro Tyr Lys Thr Gly Thr 130 135 140 Gly Ala Val Glu Pro Tyr Pro Arg Arg Met Leu Asp Ser Ser Glu Asn 145 150 155 160 Val Arg Arg Pro Glu Ile Asp Thr Gly Val Ala Leu Glu Lys Leu Arg 165 170 175 Val Lys Leu Arg Gly Pro Pro Pro Asp Gly Met Arg Asp Leu Arg Glu 180 185 190 Glu Phe Ala Val Arg Ser Val Glu Glu Val Tyr Ala Pro Val Tyr Glu 195 200 205 Ser Arg Leu Val Gly Pro Lys Lys Lys Val Arg Ile Met Arg Ile Asp 210 215 220 Ala Ala Arg Lys Lys Met Leu 225 230 <210> SEQ ID NO 19 <211> LENGTH: 378 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(378) <400> SEQUENCE: 19 atg agg tca gaa gag agg ccg ggt cac att gaa aag ttc cta aag agg 48 Met Arg Ser Glu Glu Arg Pro Gly His Ile Glu Lys Phe Leu Lys Arg 1 5 10 15 gcg gac aag gcg atc gac agc gcg gtc gag cag ggc gtc aag agg gcc 96 Ala Asp Lys Ala Ile Asp Ser Ala Val Glu Gln Gly Val Lys Arg Ala 20 25 30 gac gag ata cta gac gat gca gtc gag ctc ggc aag att acg gtg ggc 144 Asp Glu Ile Leu Asp Asp Ala Val Glu Leu Gly Lys Ile Thr Val Gly 35 40 45 gag gcg cag agg agg agc gat gtg ctg ctc aaa cag gcc gag cgg gag 192 Glu Ala Gln Arg Arg Ser Asp Val Leu Leu Lys Gln Ala Glu Arg Glu 50 55 60 agc agg cgg ctc aag tcc aag ggc gcc aaa aag ctc gaa aag ggc ata 240 Ser Arg Arg Leu Lys Ser Lys Gly Ala Lys Lys Leu Glu Lys Gly Ile 65 70 75 80 ggc gcc gca aaa aag atg gca gca ggc aag ggc gac gcg ctc gag acg 288 Gly Ala Ala Lys Lys Met Ala Ala Gly Lys Gly Asp Ala Leu Glu Thr 85 90 95 ctc gca aag ctc ggc gag ctc aga aag gcg ggg atc ata acg gag aaa 336 Leu Ala Lys Leu Gly Glu Leu Arg Lys Ala Gly Ile Ile Thr Glu Lys 100 105 110 gag ttt cgc gcc aaa aag aaa aag ctc ctc gca gag atc tga 378 Glu Phe Arg Ala Lys Lys Lys Lys Leu Leu Ala Glu Ile 115 120 125 <210> SEQ ID NO 20 <211> LENGTH: 125 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 20 Met Arg Ser Glu Glu Arg Pro Gly His Ile Glu Lys Phe Leu Lys Arg 1 5 10 15 Ala Asp Lys Ala Ile Asp Ser Ala Val Glu Gln Gly Val Lys Arg Ala 20 25 30 Asp Glu Ile Leu Asp Asp Ala Val Glu Leu Gly Lys Ile Thr Val Gly 35 40 45 Glu Ala Gln Arg Arg Ser Asp Val Leu Leu Lys Gln Ala Glu Arg Glu 50 55 60 Ser Arg Arg Leu Lys Ser Lys Gly Ala Lys Lys Leu Glu Lys Gly Ile 65 70 75 80 Gly Ala Ala Lys Lys Met Ala Ala Gly Lys Gly Asp Ala Leu Glu Thr 85 90 95 Leu Ala Lys Leu Gly Glu Leu Arg Lys Ala Gly Ile Ile Thr Glu Lys 100 105 110 Glu Phe Arg Ala Lys Lys Lys Lys Leu Leu Ala Glu Ile 115 120 125 <210> SEQ ID NO 21 <211> LENGTH: 600 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(600) <400> SEQUENCE: 21 atg tcc cag acg ggg gcc ccg ggc ggg cat gcc tgc acg cca tac acg 48 Met Ser Gln Thr Gly Ala Pro Gly Gly His Ala Cys Thr Pro Tyr Thr 1 5 10 15 cac gat cac gcc tcg atc gag ctc aag gac gcg tgg gcc tcg tcg agg 96 His Asp His Ala Ser Ile Glu Leu Lys Asp Ala Trp Ala Ser Ser Arg 20 25 30 aac gtc cgc gag atg tac ttt gtg acc gcc acg ttc tcg tcc gag agc 144 Asn Val Arg Glu Met Tyr Phe Val Thr Ala Thr Phe Ser Ser Glu Ser 35 40 45 cag ccg tac ttt gca ccg cag gcc aac cac tac ctg ctg gca agg ttc 192 Gln Pro Tyr Phe Ala Pro Gln Ala Asn His Tyr Leu Leu Ala Arg Phe 50 55 60 aag gac gcc ccc aga atg atc aag gcg gtg ggc cgg ggg gag ggc gca 240 Lys Asp Ala Pro Arg Met Ile Lys Ala Val Gly Arg Gly Glu Gly Ala 65 70 75 80 tcc tat gtg ttt agc atg gac gag gac ata ttc gag agg gag tcc ccc 288 Ser Tyr Val Phe Ser Met Asp Glu Asp Ile Phe Glu Arg Glu Ser Pro 85 90 95 ggg gtg agc tat gta tcg gtg tac tat ctg gag tac ggc gat tcc gag 336 Gly Val Ser Tyr Val Ser Val Tyr Tyr Leu Glu Tyr Gly Asp Ser Glu 100 105 110 gag gac ata tgc gag gtg gcg tcc gtg gtg ggg aga aag gag aag ata 384 Glu Asp Ile Cys Glu Val Ala Ser Val Val Gly Arg Lys Glu Lys Ile 115 120 125 ggc agg gcg gga ata ggg cgc atg gac gtc tgc tcg agg gtg ccg cca 432 Gly Arg Ala Gly Ile Gly Arg Met Asp Val Cys Ser Arg Val Pro Pro 130 135 140 aag ttt gcc ttt ccg tac agc ggg aac ata ata gtc ctc gag gtc tcc 480 Lys Phe Ala Phe Pro Tyr Ser Gly Asn Ile Ile Val Leu Glu Val Ser 145 150 155 160 agc gag aag agc tac cag agc gtc aac aag tac tgc gag aag acg cgg 528 Ser Glu Lys Ser Tyr Gln Ser Val Asn Lys Tyr Cys Glu Lys Thr Arg 165 170 175 cgc gag gtc atc cgc aag ggg ata acg atg acc aac ctt gtg agc ctg 576 Arg Glu Val Ile Arg Lys Gly Ile Thr Met Thr Asn Leu Val Ser Leu 180 185 190 tcc ata ctg gag cgg cta aag tag 600 Ser Ile Leu Glu Arg Leu Lys 195 <210> SEQ ID NO 22 <211> LENGTH: 199 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 22 Met Ser Gln Thr Gly Ala Pro Gly Gly His Ala Cys Thr Pro Tyr Thr 1 5 10 15 His Asp His Ala Ser Ile Glu Leu Lys Asp Ala Trp Ala Ser Ser Arg 20 25 30 Asn Val Arg Glu Met Tyr Phe Val Thr Ala Thr Phe Ser Ser Glu Ser 35 40 45 Gln Pro Tyr Phe Ala Pro Gln Ala Asn His Tyr Leu Leu Ala Arg Phe 50 55 60 Lys Asp Ala Pro Arg Met Ile Lys Ala Val Gly Arg Gly Glu Gly Ala 65 70 75 80 Ser Tyr Val Phe Ser Met Asp Glu Asp Ile Phe Glu Arg Glu Ser Pro 85 90 95 Gly Val Ser Tyr Val Ser Val Tyr Tyr Leu Glu Tyr Gly Asp Ser Glu 100 105 110 Glu Asp Ile Cys Glu Val Ala Ser Val Val Gly Arg Lys Glu Lys Ile 115 120 125 Gly Arg Ala Gly Ile Gly Arg Met Asp Val Cys Ser Arg Val Pro Pro 130 135 140 Lys Phe Ala Phe Pro Tyr Ser Gly Asn Ile Ile Val Leu Glu Val Ser 145 150 155 160 Ser Glu Lys Ser Tyr Gln Ser Val Asn Lys Tyr Cys Glu Lys Thr Arg 165 170 175 Arg Glu Val Ile Arg Lys Gly Ile Thr Met Thr Asn Leu Val Ser Leu 180 185 190 Ser Ile Leu Glu Arg Leu Lys 195 <210> SEQ ID NO 23 <211> LENGTH: 810 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(810) <400> SEQUENCE: 23 ttg gct cgg cgc tac aag ccc cgg ata aag cag gtc cta cgc gag gtg 48 Met Ala Arg Arg Tyr Lys Pro Arg Ile Lys Gln Val Leu Arg Glu Val 1 5 10 15 ccc ctc aag aac gtg cac gtg tgg aag gac gcg cag gca agg agg ctg 96 Pro Leu Lys Asn Val His Val Trp Lys Asp Ala Gln Ala Arg Arg Leu 20 25 30 gac agg tcc agg gtg agg gag att gca aag tcg atc agg tcc gag ggc 144 Asp Arg Ser Arg Val Arg Glu Ile Ala Lys Ser Ile Arg Ser Glu Gly 35 40 45 ctg cag aac ccg ccc gta ata cag agg ggc ggc agg ggg ctg tac ctg 192 Leu Gln Asn Pro Pro Val Ile Gln Arg Gly Gly Arg Gly Leu Tyr Leu 50 55 60 ctc ata tcg ggg aac cac agg ctt gcg gcc cta aag cat ctg ggc gca 240 Leu Ile Ser Gly Asn His Arg Leu Ala Ala Leu Lys His Leu Gly Ala 65 70 75 80 aaa aag tcc aag ttt ctt gtg ata acc aag gat acg gag tac ggc ctg 288 Lys Lys Ser Lys Phe Leu Val Ile Thr Lys Asp Thr Glu Tyr Gly Leu 85 90 95 gag gac gca aag gcg gca tcg gtc gtg gag aac ctg cac cgg atg cag 336 Glu Asp Ala Lys Ala Ala Ser Val Val Glu Asn Leu His Arg Met Gln 100 105 110 atg agc ccc cgg gag ctc gcc gac gcg tgc agg ttt ctc gcc gag cag 384 Met Ser Pro Arg Glu Leu Ala Asp Ala Cys Arg Phe Leu Ala Glu Gln 115 120 125 atg acc cgc gcc gag gcc gca agg aag ctc ggc atg tcg atg ccc acg 432 Met Thr Arg Ala Glu Ala Ala Arg Lys Leu Gly Met Ser Met Pro Thr 130 135 140 ttc aaa aag tac cac ggc ttt gcg ggc gtg ccg gag aag atc aag gcg 480 Phe Lys Lys Tyr His Gly Phe Ala Gly Val Pro Glu Lys Ile Lys Ala 145 150 155 160 cta gtc ccc ggg acc ata tcc cgg gac gag gcg aca aag ctg tac cag 528 Leu Val Pro Gly Thr Ile Ser Arg Asp Glu Ala Thr Lys Leu Tyr Gln 165 170 175 gcc gtc ccg acc gtc tcc cag gcg ctc aag gtg gcg ctg aac ata tca 576 Ala Val Pro Thr Val Ser Gln Ala Leu Lys Val Ala Leu Asn Ile Ser 180 185 190 agg ctt gat cgg ccg tcg agg cgg atc tac ctg agg ctg cta gcc cag 624 Arg Leu Asp Arg Pro Ser Arg Arg Ile Tyr Leu Arg Leu Leu Ala Gln 195 200 205 agc ccc cgc tcg ggc cac agg atc ctg cta aag agg gtg cgc aag acg 672 Ser Pro Arg Ser Gly His Arg Ile Leu Leu Lys Arg Val Arg Lys Thr 210 215 220 ggc gtc agg aag aag atc ccc ata gag ctc ggc aag aac ggc gca aga 720 Gly Val Arg Lys Lys Ile Pro Ile Glu Leu Gly Lys Asn Gly Ala Arg 225 230 235 240 aag ctt gcc cgg gtg gcc gag cgc gag ggc acc gac gag acc cgg ctt 768 Lys Leu Ala Arg Val Ala Glu Arg Glu Gly Thr Asp Glu Thr Arg Leu 245 250 255 gcc aac agg ata gtc cgg gag tac ctg agg aag cag cga tga 810 Ala Asn Arg Ile Val Arg Glu Tyr Leu Arg Lys Gln Arg 260 265 <210> SEQ ID NO 24 <211> LENGTH: 269 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 24 Met Ala Arg Arg Tyr Lys Pro Arg Ile Lys Gln Val Leu Arg Glu Val 1 5 10 15 Pro Leu Lys Asn Val His Val Trp Lys Asp Ala Gln Ala Arg Arg Leu 20 25 30 Asp Arg Ser Arg Val Arg Glu Ile Ala Lys Ser Ile Arg Ser Glu Gly 35 40 45 Leu Gln Asn Pro Pro Val Ile Gln Arg Gly Gly Arg Gly Leu Tyr Leu 50 55 60 Leu Ile Ser Gly Asn His Arg Leu Ala Ala Leu Lys His Leu Gly Ala 65 70 75 80 Lys Lys Ser Lys Phe Leu Val Ile Thr Lys Asp Thr Glu Tyr Gly Leu 85 90 95 Glu Asp Ala Lys Ala Ala Ser Val Val Glu Asn Leu His Arg Met Gln 100 105 110 Met Ser Pro Arg Glu Leu Ala Asp Ala Cys Arg Phe Leu Ala Glu Gln 115 120 125 Met Thr Arg Ala Glu Ala Ala Arg Lys Leu Gly Met Ser Met Pro Thr 130 135 140 Phe Lys Lys Tyr His Gly Phe Ala Gly Val Pro Glu Lys Ile Lys Ala 145 150 155 160 Leu Val Pro Gly Thr Ile Ser Arg Asp Glu Ala Thr Lys Leu Tyr Gln 165 170 175 Ala Val Pro Thr Val Ser Gln Ala Leu Lys Val Ala Leu Asn Ile Ser 180 185 190 Arg Leu Asp Arg Pro Ser Arg Arg Ile Tyr Leu Arg Leu Leu Ala Gln 195 200 205 Ser Pro Arg Ser Gly His Arg Ile Leu Leu Lys Arg Val Arg Lys Thr 210 215 220 Gly Val Arg Lys Lys Ile Pro Ile Glu Leu Gly Lys Asn Gly Ala Arg 225 230 235 240 Lys Leu Ala Arg Val Ala Glu Arg Glu Gly Thr Asp Glu Thr Arg Leu 245 250 255 Ala Asn Arg Ile Val Arg Glu Tyr Leu Arg Lys Gln Arg 260 265 <210> SEQ ID NO 25 <211> LENGTH: 837 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(837) <400> SEQUENCE: 25 ttg tta act gtg ttt ggt aag ttt atc aca aca att agg tta gat aga 48 Met Leu Thr Val Phe Gly Lys Phe Ile Thr Thr Ile Arg Leu Asp Arg 1 5 10 15 gct gtt ccc ccg cag gcc ccc gtg cac gta ctc tat cgc gca gcc ccc 96 Ala Val Pro Pro Gln Ala Pro Val His Val Leu Tyr Arg Ala Ala Pro 20 25 30 cgg ggg aca gcc gga acc ggg ggc tgc cgg ggc ggg atc ccg ggc gtc 144 Arg Gly Thr Ala Gly Thr Gly Gly Cys Arg Gly Gly Ile Pro Gly Val 35 40 45 gat aga ata aat acg cgc ggg gcc gcg gtg cga tcg ccc gtg ctg ata 192 Asp Arg Ile Asn Thr Arg Gly Ala Ala Val Arg Ser Pro Val Leu Ile 50 55 60 ata aac tgc aaa aac tat gag gag gcc gcc ggc ggc agg atc cgc ggg 240 Ile Asn Cys Lys Asn Tyr Glu Glu Ala Ala Gly Gly Arg Ile Arg Gly 65 70 75 80 ctg gca gat gcc gcg gcc ggg gct gcc gcc agg tac ggc gtc agg ata 288 Leu Ala Asp Ala Ala Ala Gly Ala Ala Ala Arg Tyr Gly Val Arg Ile 85 90 95 gcg ata gcc ccg ccg cag cac ctg ctg ggc att ata gca ggc cgg gat 336 Ala Ile Ala Pro Pro Gln His Leu Leu Gly Ile Ile Ala Gly Arg Asp 100 105 110 ctt ggc gtg ctg gcc cag cat gtc gac gac aag ggg acg ggg agc acc 384 Leu Gly Val Leu Ala Gln His Val Asp Asp Lys Gly Thr Gly Ser Thr 115 120 125 aca ggg tat gtc gtc ccg gag ctg cta aaa cag tcg ggg gtc tcc ggg 432 Thr Gly Tyr Val Val Pro Glu Leu Leu Lys Gln Ser Gly Val Ser Gly 130 135 140 gcc ata atc aac cac agc gag cac cgc gta ccc gcg gac cag gtg gcg 480 Ala Ile Ile Asn His Ser Glu His Arg Val Pro Ala Asp Gln Val Ala 145 150 155 160 ggc ctg gta cca agg ctc agg ggc ctt ggc atg gtc tcg gtg gtc tgc 528 Gly Leu Val Pro Arg Leu Arg Gly Leu Gly Met Val Ser Val Val Cys 165 170 175 gtc agg gat ccc gcc gag gcc gcc gat ctc tcc cgg tat tgc ccc gac 576 Val Arg Asp Pro Ala Glu Ala Ala Asp Leu Ser Arg Tyr Cys Pro Asp 180 185 190 tac ata gcg ata gag cct ccc gag ctg ata ggt tcc ggc agg tcc gtc 624 Tyr Ile Ala Ile Glu Pro Pro Glu Leu Ile Gly Ser Gly Arg Ser Val 195 200 205 tcg aca gag agg ccc cag gtc ata caa gag gcc gca gag gcc atc agg 672 Ser Thr Glu Arg Pro Gln Val Ile Gln Glu Ala Ala Glu Ala Ile Arg 210 215 220 ggg gct ggc ggc gta aag ctg ctc tgc ggg gcg ggc ata acc tcc ggg 720 Gly Ala Gly Gly Val Lys Leu Leu Cys Gly Ala Gly Ile Thr Ser Gly 225 230 235 240 gcg gac gtg cgc agg gcc ctc gag ctt ggc tcc gag ggc att ctt gtg 768 Ala Asp Val Arg Arg Ala Leu Glu Leu Gly Ser Glu Gly Ile Leu Val 245 250 255 gca agc ggg gtc gta aag tcg gca gac ccc gca ggg gcc atc ggg gag 816 Ala Ser Gly Val Val Lys Ser Ala Asp Pro Ala Gly Ala Ile Gly Glu 260 265 270 ctt gcc cgg gcc atg tcc tga 837 Leu Ala Arg Ala Met Ser 275 <210> SEQ ID NO 26 <211> LENGTH: 278 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 26 Met Leu Thr Val Phe Gly Lys Phe Ile Thr Thr Ile Arg Leu Asp Arg 1 5 10 15 Ala Val Pro Pro Gln Ala Pro Val His Val Leu Tyr Arg Ala Ala Pro 20 25 30 Arg Gly Thr Ala Gly Thr Gly Gly Cys Arg Gly Gly Ile Pro Gly Val 35 40 45 Asp Arg Ile Asn Thr Arg Gly Ala Ala Val Arg Ser Pro Val Leu Ile 50 55 60 Ile Asn Cys Lys Asn Tyr Glu Glu Ala Ala Gly Gly Arg Ile Arg Gly 65 70 75 80 Leu Ala Asp Ala Ala Ala Gly Ala Ala Ala Arg Tyr Gly Val Arg Ile 85 90 95 Ala Ile Ala Pro Pro Gln His Leu Leu Gly Ile Ile Ala Gly Arg Asp 100 105 110 Leu Gly Val Leu Ala Gln His Val Asp Asp Lys Gly Thr Gly Ser Thr 115 120 125 Thr Gly Tyr Val Val Pro Glu Leu Leu Lys Gln Ser Gly Val Ser Gly 130 135 140 Ala Ile Ile Asn His Ser Glu His Arg Val Pro Ala Asp Gln Val Ala 145 150 155 160 Gly Leu Val Pro Arg Leu Arg Gly Leu Gly Met Val Ser Val Val Cys 165 170 175 Val Arg Asp Pro Ala Glu Ala Ala Asp Leu Ser Arg Tyr Cys Pro Asp 180 185 190 Tyr Ile Ala Ile Glu Pro Pro Glu Leu Ile Gly Ser Gly Arg Ser Val 195 200 205 Ser Thr Glu Arg Pro Gln Val Ile Gln Glu Ala Ala Glu Ala Ile Arg 210 215 220 Gly Ala Gly Gly Val Lys Leu Leu Cys Gly Ala Gly Ile Thr Ser Gly 225 230 235 240 Ala Asp Val Arg Arg Ala Leu Glu Leu Gly Ser Glu Gly Ile Leu Val 245 250 255 Ala Ser Gly Val Val Lys Ser Ala Asp Pro Ala Gly Ala Ile Gly Glu 260 265 270 Leu Ala Arg Ala Met Ser 275 <210> SEQ ID NO 27 <211> LENGTH: 549 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(549) <400> SEQUENCE: 27 atg ctg gat cca agg aaa cgg ccc agg gtg gtc aac gtt gtg agt acc 48 Met Leu Asp Pro Arg Lys Arg Pro Arg Val Val Asn Val Val Ser Thr 1 5 10 15 gcc gac ctg ggc cgg agg gtg ggc gca aaa aag atg gcc gcc atg cca 96 Ala Asp Leu Gly Arg Arg Val Gly Ala Lys Lys Met Ala Ala Met Pro 20 25 30 tgc tgc atg tac gac gag gcg gta tac ggc ggc agg tgc ggc tat atc 144 Cys Cys Met Tyr Asp Glu Ala Val Tyr Gly Gly Arg Cys Gly Tyr Ile 35 40 45 aaa aca ccc ggc atg cgg ggg cgc gtg acg gtg ttt ctc tcg ggc aag 192 Lys Thr Pro Gly Met Arg Gly Arg Val Thr Val Phe Leu Ser Gly Lys 50 55 60 atg ata tcc gtc ggc gcc agc tcc gtg agg gca tcg ttt gcg cag ctg 240 Met Ile Ser Val Gly Ala Ser Ser Val Arg Ala Ser Phe Ala Gln Leu 65 70 75 80 cac gag gcc cgg ctg cac ctg ttc cgg aac ggg gcg gcg gcc ggc ggg 288 His Glu Ala Arg Leu His Leu Phe Arg Asn Gly Ala Ala Ala Gly Gly 85 90 95 tgt aca agg ccc gtc gta cgc aat atg gtg gcg aca gtg gat gca gga 336 Cys Thr Arg Pro Val Val Arg Asn Met Val Ala Thr Val Asp Ala Gly 100 105 110 cgg act gtt ccc ata gac agg ata tcg tcg cgg ata ccc ggc gcg gtg 384 Arg Thr Val Pro Ile Asp Arg Ile Ser Ser Arg Ile Pro Gly Ala Val 115 120 125 tac gac ccg ggg tcg ttt ccc ggc atg ata cta aag ggg ctg ggc agc 432 Tyr Asp Pro Gly Ser Phe Pro Gly Met Ile Leu Lys Gly Leu Gly Ser 130 135 140 tgc agc ttc ctt gtg ttt gcg tcg gga aag gtg gtg ata gcg ggc gcc 480 Cys Ser Phe Leu Val Phe Ala Ser Gly Lys Val Val Ile Ala Gly Ala 145 150 155 160 cgg tcg cca ggc gag cta tac agg tcg tcg ttt gac ctg ctg gcg cgc 528 Arg Ser Pro Gly Glu Leu Tyr Arg Ser Ser Phe Asp Leu Leu Ala Arg 165 170 175 ctc aac ggc gcg ggc gcc tag 549 Leu Asn Gly Ala Gly Ala 180 <210> SEQ ID NO 28 <211> LENGTH: 182 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 28 Met Leu Asp Pro Arg Lys Arg Pro Arg Val Val Asn Val Val Ser Thr 1 5 10 15 Ala Asp Leu Gly Arg Arg Val Gly Ala Lys Lys Met Ala Ala Met Pro 20 25 30 Cys Cys Met Tyr Asp Glu Ala Val Tyr Gly Gly Arg Cys Gly Tyr Ile 35 40 45 Lys Thr Pro Gly Met Arg Gly Arg Val Thr Val Phe Leu Ser Gly Lys 50 55 60 Met Ile Ser Val Gly Ala Ser Ser Val Arg Ala Ser Phe Ala Gln Leu 65 70 75 80 His Glu Ala Arg Leu His Leu Phe Arg Asn Gly Ala Ala Ala Gly Gly 85 90 95 Cys Thr Arg Pro Val Val Arg Asn Met Val Ala Thr Val Asp Ala Gly 100 105 110 Arg Thr Val Pro Ile Asp Arg Ile Ser Ser Arg Ile Pro Gly Ala Val 115 120 125 Tyr Asp Pro Gly Ser Phe Pro Gly Met Ile Leu Lys Gly Leu Gly Ser 130 135 140 Cys Ser Phe Leu Val Phe Ala Ser Gly Lys Val Val Ile Ala Gly Ala 145 150 155 160 Arg Ser Pro Gly Glu Leu Tyr Arg Ser Ser Phe Asp Leu Leu Ala Arg 165 170 175 Leu Asn Gly Ala Gly Ala 180 <210> SEQ ID NO 29 <211> LENGTH: 2535 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(2535) <400> SEQUENCE: 29 gtg acg gtg caa gat gcc gta gag ata ccc ccg tcg ctg ctg gta tct 48 Met Thr Val Gln Asp Ala Val Glu Ile Pro Pro Ser Leu Leu Val Ser 1 5 10 15 gca aca tac gac agc cag gca ggg gcg gtc gtc ctc aag ttt tac gag 96 Ala Thr Tyr Asp Ser Gln Ala Gly Ala Val Val Leu Lys Phe Tyr Glu 20 25 30 ccg gaa tca caa aag atc gta cac tgg acg gac aat acg ggg cac aag 144 Pro Glu Ser Gln Lys Ile Val His Trp Thr Asp Asn Thr Gly His Lys 35 40 45 ccc tac tgc tat acg agg cag ccc ccc tcc gag ctt ggg gag ctt gaa 192 Pro Tyr Cys Tyr Thr Arg Gln Pro Pro Ser Glu Leu Gly Glu Leu Glu 50 55 60 ggc agg gag gat gtg cta gga acg gag cag gtc atg cgg cac gac ctg 240 Gly Arg Glu Asp Val Leu Gly Thr Glu Gln Val Met Arg His Asp Leu 65 70 75 80 ata gcc gac aag gat gtg ccc gtc acc aag ata act gtg gcc gac ccc 288 Ile Ala Asp Lys Asp Val Pro Val Thr Lys Ile Thr Val Ala Asp Pro 85 90 95 ctt gcc ata ggc ggg acc aac tcg gag aag agc atc cgc aac atc atg 336 Leu Ala Ile Gly Gly Thr Asn Ser Glu Lys Ser Ile Arg Asn Ile Met 100 105 110 gac acg tgg gaa tcc gac ata aag tac tat gag aac tat ctg tac gac 384 Asp Thr Trp Glu Ser Asp Ile Lys Tyr Tyr Glu Asn Tyr Leu Tyr Asp 115 120 125 aag agc ctg gtc gtg ggc agg tac tat tcg gta tcc ggc ggc aag gta 432 Lys Ser Leu Val Val Gly Arg Tyr Tyr Ser Val Ser Gly Gly Lys Val 130 135 140 atc ccg cat gac atg ccc ata tcc gac gag gta aag ctg gcc ctc aag 480 Ile Pro His Asp Met Pro Ile Ser Asp Glu Val Lys Leu Ala Leu Lys 145 150 155 160 agc ctc ctc tgg gac aag gtt gta gac gag ggc atg gcg gac aga aaa 528 Ser Leu Leu Trp Asp Lys Val Val Asp Glu Gly Met Ala Asp Arg Lys 165 170 175 gag ttc cgc gag ttc ata gcg ggg tgg gcg gac ctg ctc aac cag ccc 576 Glu Phe Arg Glu Phe Ile Ala Gly Trp Ala Asp Leu Leu Asn Gln Pro 180 185 190 ata ccc agg ata cgg cgc ctc agc ttt gat atc gag gtg gat tca gag 624 Ile Pro Arg Ile Arg Arg Leu Ser Phe Asp Ile Glu Val Asp Ser Glu 195 200 205 gag ggc agg atc ccc gac ccc aag ata tcc gac agg agg gtt acg gcg 672 Glu Gly Arg Ile Pro Asp Pro Lys Ile Ser Asp Arg Arg Val Thr Ala 210 215 220 gtg ggg ttt gcc gcc acc gac ggc cta aaa cag gta ttc gtc ctg agg 720 Val Gly Phe Ala Ala Thr Asp Gly Leu Lys Gln Val Phe Val Leu Arg 225 230 235 240 agc ggc gca gaa gag ggc gag aac ggc gtg acc ccc ggt gtc gag gtg 768 Ser Gly Ala Glu Glu Gly Glu Asn Gly Val Thr Pro Gly Val Glu Val 245 250 255 gta ttc tac gac aag gaa gct gac atg atc cgc gac gcg cta tcg gta 816 Val Phe Tyr Asp Lys Glu Ala Asp Met Ile Arg Asp Ala Leu Ser Val 260 265 270 ata ggc tcg tac ccg ttt gtt ctg acg tac aac ggc gac gac ttt gac 864 Ile Gly Ser Tyr Pro Phe Val Leu Thr Tyr Asn Gly Asp Asp Phe Asp 275 280 285 atg ccg tac atg ctc aac agg gca cgg cgc ctc gga gta tct gac tct 912 Met Pro Tyr Met Leu Asn Arg Ala Arg Arg Leu Gly Val Ser Asp Ser 290 295 300 gac att cct ttg tac atg atg cgg gat tct gcc acg ctc cgg cac gga 960 Asp Ile Pro Leu Tyr Met Met Arg Asp Ser Ala Thr Leu Arg His Gly 305 310 315 320 gtc cac ctg gac ctg tac agg acc ttc tcg aac agg tca ttc cag ctg 1008 Val His Leu Asp Leu Tyr Arg Thr Phe Ser Asn Arg Ser Phe Gln Leu 325 330 335 tac gcc ttt gcg gca aag tac acg gac tat tcc ctt aac agc gtc aca 1056 Tyr Ala Phe Ala Ala Lys Tyr Thr Asp Tyr Ser Leu Asn Ser Val Thr 340 345 350 aag gcg atg ctc ggc gag ggc aag gtc gac tat ggg gtc aaa ctg ggg 1104 Lys Ala Met Leu Gly Glu Gly Lys Val Asp Tyr Gly Val Lys Leu Gly 355 360 365 gat ctc acc tta tac cag act gca aac tat tgc tat cac gac gcg cgc 1152 Asp Leu Thr Leu Tyr Gln Thr Ala Asn Tyr Cys Tyr His Asp Ala Arg 370 375 380 ctg acg ctc gag ctt agc acc ttt ggc aac gag ata ctc atg gac ctg 1200 Leu Thr Leu Glu Leu Ser Thr Phe Gly Asn Glu Ile Leu Met Asp Leu 385 390 395 400 ctg gtg gtg acc agc aga ata gcc cgg atg ccc atc gat gac atg tcc 1248 Leu Val Val Thr Ser Arg Ile Ala Arg Met Pro Ile Asp Asp Met Ser 405 410 415 cgc atg ggc gtc tcg cag tgg ata cgc agc ctg ctg tac tat gag cac 1296 Arg Met Gly Val Ser Gln Trp Ile Arg Ser Leu Leu Tyr Tyr Glu His 420 425 430 aga cag cga aac gcg ctc ata ccg cgg agg gac gag ctg gag ggc agg 1344 Arg Gln Arg Asn Ala Leu Ile Pro Arg Arg Asp Glu Leu Glu Gly Arg 435 440 445 tcg cgc gag gtg agc aac gac gcg gta ata aag gat aaa aag ttc cgc 1392 Ser Arg Glu Val Ser Asn Asp Ala Val Ile Lys Asp Lys Lys Phe Arg 450 455 460 ggg ggc ctt gtc gtc gag cct gaa gag ggc ata cac ttt gat gtt acg 1440 Gly Gly Leu Val Val Glu Pro Glu Glu Gly Ile His Phe Asp Val Thr 465 470 475 480 gtg atg gac ttt gcg agc ctg tat ccc agt atc ata aag gtg agg aac 1488 Val Met Asp Phe Ala Ser Leu Tyr Pro Ser Ile Ile Lys Val Arg Asn 485 490 495 ctc tcg tac gag acc gtc cgg tgc gtg cat gca gaa tgc aaa aag aac 1536 Leu Ser Tyr Glu Thr Val Arg Cys Val His Ala Glu Cys Lys Lys Asn 500 505 510 acc atc ccc gat acc aac cac tgg gta tgt aca aaa aac aac ggc ctg 1584 Thr Ile Pro Asp Thr Asn His Trp Val Cys Thr Lys Asn Asn Gly Leu 515 520 525 aca tcg atg ata atc ggc tcg ctg cgg gac ctg cgc gtc aac tat tac 1632 Thr Ser Met Ile Ile Gly Ser Leu Arg Asp Leu Arg Val Asn Tyr Tyr 530 535 540 aag agc ctc tca aag agc aca tcc att acg gag gag cag cgg cag cag 1680 Lys Ser Leu Ser Lys Ser Thr Ser Ile Thr Glu Glu Gln Arg Gln Gln 545 550 555 560 tat acc gta atc agc cag gcc ctc aag gtc gtg ctc aac gca agc tac 1728 Tyr Thr Val Ile Ser Gln Ala Leu Lys Val Val Leu Asn Ala Ser Tyr 565 570 575 ggc gtg atg ggc gcc gag ata ttc ccg ctg tac ttt tta ccc gcg gca 1776 Gly Val Met Gly Ala Glu Ile Phe Pro Leu Tyr Phe Leu Pro Ala Ala 580 585 590 gag gcc acc act gct gtc ggg cgc tat atc atc atg cag acg ata tcg 1824 Glu Ala Thr Thr Ala Val Gly Arg Tyr Ile Ile Met Gln Thr Ile Ser 595 600 605 cac tgc gag cag atg gga gtg agg gtg ctg tac ggg gac acc gat tct 1872 His Cys Glu Gln Met Gly Val Arg Val Leu Tyr Gly Asp Thr Asp Ser 610 615 620 ctg ttc ata aag gat ccc gaa gag agg cag atc cac gag ata gtc gag 1920 Leu Phe Ile Lys Asp Pro Glu Glu Arg Gln Ile His Glu Ile Val Glu 625 630 635 640 cat gca aag aag gag cac ggt gtg gag ctc gaa gtg gac aaa gag tac 1968 His Ala Lys Lys Glu His Gly Val Glu Leu Glu Val Asp Lys Glu Tyr 645 650 655 agg tat gtc gtg cta tcc aac agg aaa aaa aac tat ttc ggg gtg acc 2016 Arg Tyr Val Val Leu Ser Asn Arg Lys Lys Asn Tyr Phe Gly Val Thr 660 665 670 cgg gca ggc aag gtc gac gtc aag ggg ctg acg ggc aaa aag tcg cac 2064 Arg Ala Gly Lys Val Asp Val Lys Gly Leu Thr Gly Lys Lys Ser His 675 680 685 acg ccc ccg ttc ata aag gag ctc ttc tac tcg ctg ctc gac ata ctc 2112 Thr Pro Pro Phe Ile Lys Glu Leu Phe Tyr Ser Leu Leu Asp Ile Leu 690 695 700 tca gga gtc gag agc gag gac gag ttc gag tca gcc aag atg agg atc 2160 Ser Gly Val Glu Ser Glu Asp Glu Phe Glu Ser Ala Lys Met Arg Ile 705 710 715 720 tca aag gcg atc gcc gcg tgc ggc aag agg ctc gag gag agg cag atc 2208 Ser Lys Ala Ile Ala Ala Cys Gly Lys Arg Leu Glu Glu Arg Gln Ile 725 730 735 ccc ctc gtg gac ctg gcg ttc aat gtg atg ata agc aag gcg ccc tcc 2256 Pro Leu Val Asp Leu Ala Phe Asn Val Met Ile Ser Lys Ala Pro Ser 740 745 750 gaa tat gtc aag acc gtc ccg cag cac ata cgg gcg gca agg ctg ctg 2304 Glu Tyr Val Lys Thr Val Pro Gln His Ile Arg Ala Ala Arg Leu Leu 755 760 765 gag aac gca agg gag gtc aaa aag ggc gac ata ata tcg tac gta aag 2352 Glu Asn Ala Arg Glu Val Lys Lys Gly Asp Ile Ile Ser Tyr Val Lys 770 775 780 gtg atg aac aag acc ggc gtc aag ccg gtg gag atg gcc cgg gca ggc 2400 Val Met Asn Lys Thr Gly Val Lys Pro Val Glu Met Ala Arg Ala Gly 785 790 795 800 gag gtg gac acg tca aag tac ctc gag ttc atg gag tcg acg ctc gac 2448 Glu Val Asp Thr Ser Lys Tyr Leu Glu Phe Met Glu Ser Thr Leu Asp 805 810 815 cag ctc acc tcg tcc atg ggc ctt gac ttt gac gag ata ctc ggc aag 2496 Gln Leu Thr Ser Ser Met Gly Leu Asp Phe Asp Glu Ile Leu Gly Lys 820 825 830 cca aag cag acc ggc atg gag cag ttc ttt ttc aaa tga 2535 Pro Lys Gln Thr Gly Met Glu Gln Phe Phe Phe Lys 835 840 <210> SEQ ID NO 30 <211> LENGTH: 844 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 30 Met Thr Val Gln Asp Ala Val Glu Ile Pro Pro Ser Leu Leu Val Ser 1 5 10 15 Ala Thr Tyr Asp Ser Gln Ala Gly Ala Val Val Leu Lys Phe Tyr Glu 20 25 30 Pro Glu Ser Gln Lys Ile Val His Trp Thr Asp Asn Thr Gly His Lys 35 40 45 Pro Tyr Cys Tyr Thr Arg Gln Pro Pro Ser Glu Leu Gly Glu Leu Glu 50 55 60 Gly Arg Glu Asp Val Leu Gly Thr Glu Gln Val Met Arg His Asp Leu 65 70 75 80 Ile Ala Asp Lys Asp Val Pro Val Thr Lys Ile Thr Val Ala Asp Pro 85 90 95 Leu Ala Ile Gly Gly Thr Asn Ser Glu Lys Ser Ile Arg Asn Ile Met 100 105 110 Asp Thr Trp Glu Ser Asp Ile Lys Tyr Tyr Glu Asn Tyr Leu Tyr Asp 115 120 125 Lys Ser Leu Val Val Gly Arg Tyr Tyr Ser Val Ser Gly Gly Lys Val 130 135 140 Ile Pro His Asp Met Pro Ile Ser Asp Glu Val Lys Leu Ala Leu Lys 145 150 155 160 Ser Leu Leu Trp Asp Lys Val Val Asp Glu Gly Met Ala Asp Arg Lys 165 170 175 Glu Phe Arg Glu Phe Ile Ala Gly Trp Ala Asp Leu Leu Asn Gln Pro 180 185 190 Ile Pro Arg Ile Arg Arg Leu Ser Phe Asp Ile Glu Val Asp Ser Glu 195 200 205 Glu Gly Arg Ile Pro Asp Pro Lys Ile Ser Asp Arg Arg Val Thr Ala 210 215 220 Val Gly Phe Ala Ala Thr Asp Gly Leu Lys Gln Val Phe Val Leu Arg 225 230 235 240 Ser Gly Ala Glu Glu Gly Glu Asn Gly Val Thr Pro Gly Val Glu Val 245 250 255 Val Phe Tyr Asp Lys Glu Ala Asp Met Ile Arg Asp Ala Leu Ser Val 260 265 270 Ile Gly Ser Tyr Pro Phe Val Leu Thr Tyr Asn Gly Asp Asp Phe Asp 275 280 285 Met Pro Tyr Met Leu Asn Arg Ala Arg Arg Leu Gly Val Ser Asp Ser 290 295 300 Asp Ile Pro Leu Tyr Met Met Arg Asp Ser Ala Thr Leu Arg His Gly 305 310 315 320 Val His Leu Asp Leu Tyr Arg Thr Phe Ser Asn Arg Ser Phe Gln Leu 325 330 335 Tyr Ala Phe Ala Ala Lys Tyr Thr Asp Tyr Ser Leu Asn Ser Val Thr 340 345 350 Lys Ala Met Leu Gly Glu Gly Lys Val Asp Tyr Gly Val Lys Leu Gly 355 360 365 Asp Leu Thr Leu Tyr Gln Thr Ala Asn Tyr Cys Tyr His Asp Ala Arg 370 375 380 Leu Thr Leu Glu Leu Ser Thr Phe Gly Asn Glu Ile Leu Met Asp Leu 385 390 395 400 Leu Val Val Thr Ser Arg Ile Ala Arg Met Pro Ile Asp Asp Met Ser 405 410 415 Arg Met Gly Val Ser Gln Trp Ile Arg Ser Leu Leu Tyr Tyr Glu His 420 425 430 Arg Gln Arg Asn Ala Leu Ile Pro Arg Arg Asp Glu Leu Glu Gly Arg 435 440 445 Ser Arg Glu Val Ser Asn Asp Ala Val Ile Lys Asp Lys Lys Phe Arg 450 455 460 Gly Gly Leu Val Val Glu Pro Glu Glu Gly Ile His Phe Asp Val Thr 465 470 475 480 Val Met Asp Phe Ala Ser Leu Tyr Pro Ser Ile Ile Lys Val Arg Asn 485 490 495 Leu Ser Tyr Glu Thr Val Arg Cys Val His Ala Glu Cys Lys Lys Asn 500 505 510 Thr Ile Pro Asp Thr Asn His Trp Val Cys Thr Lys Asn Asn Gly Leu 515 520 525 Thr Ser Met Ile Ile Gly Ser Leu Arg Asp Leu Arg Val Asn Tyr Tyr 530 535 540 Lys Ser Leu Ser Lys Ser Thr Ser Ile Thr Glu Glu Gln Arg Gln Gln 545 550 555 560 Tyr Thr Val Ile Ser Gln Ala Leu Lys Val Val Leu Asn Ala Ser Tyr 565 570 575 Gly Val Met Gly Ala Glu Ile Phe Pro Leu Tyr Phe Leu Pro Ala Ala 580 585 590 Glu Ala Thr Thr Ala Val Gly Arg Tyr Ile Ile Met Gln Thr Ile Ser 595 600 605 His Cys Glu Gln Met Gly Val Arg Val Leu Tyr Gly Asp Thr Asp Ser 610 615 620 Leu Phe Ile Lys Asp Pro Glu Glu Arg Gln Ile His Glu Ile Val Glu 625 630 635 640 His Ala Lys Lys Glu His Gly Val Glu Leu Glu Val Asp Lys Glu Tyr 645 650 655 Arg Tyr Val Val Leu Ser Asn Arg Lys Lys Asn Tyr Phe Gly Val Thr 660 665 670 Arg Ala Gly Lys Val Asp Val Lys Gly Leu Thr Gly Lys Lys Ser His 675 680 685 Thr Pro Pro Phe Ile Lys Glu Leu Phe Tyr Ser Leu Leu Asp Ile Leu 690 695 700 Ser Gly Val Glu Ser Glu Asp Glu Phe Glu Ser Ala Lys Met Arg Ile 705 710 715 720 Ser Lys Ala Ile Ala Ala Cys Gly Lys Arg Leu Glu Glu Arg Gln Ile 725 730 735 Pro Leu Val Asp Leu Ala Phe Asn Val Met Ile Ser Lys Ala Pro Ser 740 745 750 Glu Tyr Val Lys Thr Val Pro Gln His Ile Arg Ala Ala Arg Leu Leu 755 760 765 Glu Asn Ala Arg Glu Val Lys Lys Gly Asp Ile Ile Ser Tyr Val Lys 770 775 780 Val Met Asn Lys Thr Gly Val Lys Pro Val Glu Met Ala Arg Ala Gly 785 790 795 800 Glu Val Asp Thr Ser Lys Tyr Leu Glu Phe Met Glu Ser Thr Leu Asp 805 810 815 Gln Leu Thr Ser Ser Met Gly Leu Asp Phe Asp Glu Ile Leu Gly Lys 820 825 830 Pro Lys Gln Thr Gly Met Glu Gln Phe Phe Phe Lys 835 840 <210> SEQ ID NO 31 <211> LENGTH: 555 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(555) <400> SEQUENCE: 31 atg ccg ggc ggg ggc agg ctg ccc gtg agc ggc ttt gag cgc cct acc 48 Met Pro Gly Gly Gly Arg Leu Pro Val Ser Gly Phe Glu Arg Pro Thr 1 5 10 15 tgg gat gaa tat ttc atg ctg cag gcg gag ctt gca aag ctc cga tcc 96 Trp Asp Glu Tyr Phe Met Leu Gln Ala Glu Leu Ala Lys Leu Arg Ser 20 25 30 aac tgt ata gtc cgc aag gtg ggg gcc gta ata gtg agg gac cac cgg 144 Asn Cys Ile Val Arg Lys Val Gly Ala Val Ile Val Arg Asp His Arg 35 40 45 cag ctc gcc aca ggg tat aac ggg acg cct cct ggc gtc aag aac tgc 192 Gln Leu Ala Thr Gly Tyr Asn Gly Thr Pro Pro Gly Val Lys Asn Cys 50 55 60 tac gag ggc ggc tgc gag agg tgt gcc gag cgc atc gag ggc agg atc 240 Tyr Glu Gly Gly Cys Glu Arg Cys Ala Glu Arg Ile Glu Gly Arg Ile 65 70 75 80 aag tca ggc gag gcc ctg gac cgg tgc ctg tgc aac cat gca gag gcc 288 Lys Ser Gly Glu Ala Leu Asp Arg Cys Leu Cys Asn His Ala Glu Ala 85 90 95 aac gct ata atg cac tgt gcg ata ctc ggg ata ggc gcg ggg ggc ggg 336 Asn Ala Ile Met His Cys Ala Ile Leu Gly Ile Gly Ala Gly Gly Gly 100 105 110 ggg gcc acc atg tac acc acg ttc tcg ccg tgt ctg gag tgt acc aag 384 Gly Ala Thr Met Tyr Thr Thr Phe Ser Pro Cys Leu Glu Cys Thr Lys 115 120 125 atg gcc gta acg ata ggg atc agg cgg ttt gtc tgc ctt gat acc tac 432 Met Ala Val Thr Ile Gly Ile Arg Arg Phe Val Cys Leu Asp Thr Tyr 130 135 140 ccc gag aac acc tcc cgg ctg gta aaa gag aca tcc tcc gag ata acc 480 Pro Glu Asn Thr Ser Arg Leu Val Lys Glu Thr Ser Ser Glu Ile Thr 145 150 155 160 atg atg gac aag gaa aag atc tcg tac tgg gcg tca agg atg ccc gga 528 Met Met Asp Lys Glu Lys Ile Ser Tyr Trp Ala Ser Arg Met Pro Gly 165 170 175 ggc agc aag gag gtg ccg gtg cgg tga 555 Gly Ser Lys Glu Val Pro Val Arg 180 <210> SEQ ID NO 32 <211> LENGTH: 184 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 32 Met Pro Gly Gly Gly Arg Leu Pro Val Ser Gly Phe Glu Arg Pro Thr 1 5 10 15 Trp Asp Glu Tyr Phe Met Leu Gln Ala Glu Leu Ala Lys Leu Arg Ser 20 25 30 Asn Cys Ile Val Arg Lys Val Gly Ala Val Ile Val Arg Asp His Arg 35 40 45 Gln Leu Ala Thr Gly Tyr Asn Gly Thr Pro Pro Gly Val Lys Asn Cys 50 55 60 Tyr Glu Gly Gly Cys Glu Arg Cys Ala Glu Arg Ile Glu Gly Arg Ile 65 70 75 80 Lys Ser Gly Glu Ala Leu Asp Arg Cys Leu Cys Asn His Ala Glu Ala 85 90 95 Asn Ala Ile Met His Cys Ala Ile Leu Gly Ile Gly Ala Gly Gly Gly 100 105 110 Gly Ala Thr Met Tyr Thr Thr Phe Ser Pro Cys Leu Glu Cys Thr Lys 115 120 125 Met Ala Val Thr Ile Gly Ile Arg Arg Phe Val Cys Leu Asp Thr Tyr 130 135 140 Pro Glu Asn Thr Ser Arg Leu Val Lys Glu Thr Ser Ser Glu Ile Thr 145 150 155 160 Met Met Asp Lys Glu Lys Ile Ser Tyr Trp Ala Ser Arg Met Pro Gly 165 170 175 Gly Ser Lys Glu Val Pro Val Arg 180 <210> SEQ ID NO 33 <211> LENGTH: 1509 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1509) <400> SEQUENCE: 33 gtg gag act ggg cac ata acg ggc agg tac atc gag ccc ggt gcc gtc 48 Met Glu Thr Gly His Ile Thr Gly Arg Tyr Ile Glu Pro Gly Ala Val 1 5 10 15 gag agg cgc gac tac cag gtg ggc ctg gcg gaa cag gcc ata cgg gag 96 Glu Arg Arg Asp Tyr Gln Val Gly Leu Ala Glu Gln Ala Ile Arg Glu 20 25 30 aac tgt atc gtg gtg ctc ccg acg ggc ctc ggc aag act gcc gtc gcc 144 Asn Cys Ile Val Val Leu Pro Thr Gly Leu Gly Lys Thr Ala Val Ala 35 40 45 ctc cag gtg atc gcc cac tat ctc gac gag ggc cgc ggg gcg ctc ttc 192 Leu Gln Val Ile Ala His Tyr Leu Asp Glu Gly Arg Gly Ala Leu Phe 50 55 60 ctt gcc cct aca agg gtc ctg gta aac cag cac cgc cag ttc ctg ggc 240 Leu Ala Pro Thr Arg Val Leu Val Asn Gln His Arg Gln Phe Leu Gly 65 70 75 80 agg gcc ctt acc ata tcc gat att aca ctg gtc acg gga gag gac acc 288 Arg Ala Leu Thr Ile Ser Asp Ile Thr Leu Val Thr Gly Glu Asp Thr 85 90 95 att ccc cgg cgc aaa aag gcg tgg gga ggc agc gtg atc tgc gcc acg 336 Ile Pro Arg Arg Lys Lys Ala Trp Gly Gly Ser Val Ile Cys Ala Thr 100 105 110 ccc gag ata gca aga aat gat ata gag cgc ggc ctg gtc ccg ctc gaa 384 Pro Glu Ile Ala Arg Asn Asp Ile Glu Arg Gly Leu Val Pro Leu Glu 115 120 125 cag ttc ggc ctg gtc ata ttc gac gag gcc cac agg gcg gtg ggc gac 432 Gln Phe Gly Leu Val Ile Phe Asp Glu Ala His Arg Ala Val Gly Asp 130 135 140 tat gcc tat tct tcc ata gcg cgg gcg gta ggg gat aac tcc agg atg 480 Tyr Ala Tyr Ser Ser Ile Ala Arg Ala Val Gly Asp Asn Ser Arg Met 145 150 155 160 gtg ggc atg act gcg acg ctt ccc agc gag agg gag aag gca gac gag 528 Val Gly Met Thr Ala Thr Leu Pro Ser Glu Arg Glu Lys Ala Asp Glu 165 170 175 ata atg ggc acc ctg ctc tcc agg agc ata gcc cag agg aca gaa gac 576 Ile Met Gly Thr Leu Leu Ser Arg Ser Ile Ala Gln Arg Thr Glu Asp 180 185 190 gac ccg gac gta aag ccc tat gta cag gag act gcc acc gag tgg ata 624 Asp Pro Asp Val Lys Pro Tyr Val Gln Glu Thr Ala Thr Glu Trp Ile 195 200 205 aag gtg gat ctt ccc ccc gag atg aag gag ata cag agg ctc ctc aag 672 Lys Val Asp Leu Pro Pro Glu Met Lys Glu Ile Gln Arg Leu Leu Lys 210 215 220 ctg gcc ctc gac gag agg tat tcc tcc ctc aag agg tgc ggg tac gat 720 Leu Ala Leu Asp Glu Arg Tyr Ser Ser Leu Lys Arg Cys Gly Tyr Asp 225 230 235 240 ctt ggc tcg aac agg tcg ctc tcg gcg ctg ctc cgg ctg cgc atg gtg 768 Leu Gly Ser Asn Arg Ser Leu Ser Ala Leu Leu Arg Leu Arg Met Val 245 250 255 gtg ctt ggc ggc aac agg cgc gcg gcc aag ccg ctg ttc act gcg ata 816 Val Leu Gly Gly Asn Arg Arg Ala Ala Lys Pro Leu Phe Thr Ala Ile 260 265 270 cgc ata acg tac gcg cta aac ata ttc gag gcg cac ggg gtc acg ccc 864 Arg Ile Thr Tyr Ala Leu Asn Ile Phe Glu Ala His Gly Val Thr Pro 275 280 285 ttt cta aag ttc tgc gag agg acc tcc aag aaa aag ggc gtc ggc gtg 912 Phe Leu Lys Phe Cys Glu Arg Thr Ser Lys Lys Lys Gly Val Gly Val 290 295 300 gcg gag ctg ttc gaa cag gac cgg aac ttt aca ggg gcc atc gcg cgc 960 Ala Glu Leu Phe Glu Gln Asp Arg Asn Phe Thr Gly Ala Ile Ala Arg 305 310 315 320 gca aag gcc gcg cag gcg gca ggc atg gag cat ccc aag ata cca aag 1008 Ala Lys Ala Ala Gln Ala Ala Gly Met Glu His Pro Lys Ile Pro Lys 325 330 335 ctc gag gat gcc gtc cgc ggg gcc cgg gga aag gcg ctg gtc ttt acg 1056 Leu Glu Asp Ala Val Arg Gly Ala Arg Gly Lys Ala Leu Val Phe Thr 340 345 350 agc tat cgt gat tct gtc gac ctc ata cac tca aga ctc aag gcg gcc 1104 Ser Tyr Arg Asp Ser Val Asp Leu Ile His Ser Arg Leu Lys Ala Ala 355 360 365 ggg ata aac tcg ggc atc ctg ata gga aag gcg gga gaa aag ggc cta 1152 Gly Ile Asn Ser Gly Ile Leu Ile Gly Lys Ala Gly Glu Lys Gly Leu 370 375 380 aag cag aga aaa cag gtg gag act gtg gca aag ttc cgt gac ggc ggg 1200 Lys Gln Arg Lys Gln Val Glu Thr Val Ala Lys Phe Arg Asp Gly Gly 385 390 395 400 tac gac gtg ctg gta tcg acg agg gtc ggc gag gag ggg ctc gac ata 1248 Tyr Asp Val Leu Val Ser Thr Arg Val Gly Glu Glu Gly Leu Asp Ile 405 410 415 tcg gag gtc aac ctg gtg ata ttc tat gac aat gtg cca agc tcg atc 1296 Ser Glu Val Asn Leu Val Ile Phe Tyr Asp Asn Val Pro Ser Ser Ile 420 425 430 agg tac gtg cag agg agg ggg aga aca ggc aga aag gac gcc ggc agg 1344 Arg Tyr Val Gln Arg Arg Gly Arg Thr Gly Arg Lys Asp Ala Gly Arg 435 440 445 ctg ata gta ttg atg gca aag ggg acg ata gac gag gca tac tat tgg 1392 Leu Ile Val Leu Met Ala Lys Gly Thr Ile Asp Glu Ala Tyr Tyr Trp 450 455 460 att ggt cgg cgc aag atg agc gcc gcc aag ggc atg ggt gag agg atg 1440 Ile Gly Arg Arg Lys Met Ser Ala Ala Lys Gly Met Gly Glu Arg Met 465 470 475 480 aac cgg tcg ctg gcg gca ggc ggg gct gct gcc aag gcc gct cca aag 1488 Asn Arg Ser Leu Ala Ala Gly Gly Ala Ala Ala Lys Ala Ala Pro Lys 485 490 495 gga ctc gag ggg tac ttt tag 1509 Gly Leu Glu Gly Tyr Phe 500 <210> SEQ ID NO 34 <211> LENGTH: 502 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 34 Met Glu Thr Gly His Ile Thr Gly Arg Tyr Ile Glu Pro Gly Ala Val 1 5 10 15 Glu Arg Arg Asp Tyr Gln Val Gly Leu Ala Glu Gln Ala Ile Arg Glu 20 25 30 Asn Cys Ile Val Val Leu Pro Thr Gly Leu Gly Lys Thr Ala Val Ala 35 40 45 Leu Gln Val Ile Ala His Tyr Leu Asp Glu Gly Arg Gly Ala Leu Phe 50 55 60 Leu Ala Pro Thr Arg Val Leu Val Asn Gln His Arg Gln Phe Leu Gly 65 70 75 80 Arg Ala Leu Thr Ile Ser Asp Ile Thr Leu Val Thr Gly Glu Asp Thr 85 90 95 Ile Pro Arg Arg Lys Lys Ala Trp Gly Gly Ser Val Ile Cys Ala Thr 100 105 110 Pro Glu Ile Ala Arg Asn Asp Ile Glu Arg Gly Leu Val Pro Leu Glu 115 120 125 Gln Phe Gly Leu Val Ile Phe Asp Glu Ala His Arg Ala Val Gly Asp 130 135 140 Tyr Ala Tyr Ser Ser Ile Ala Arg Ala Val Gly Asp Asn Ser Arg Met 145 150 155 160 Val Gly Met Thr Ala Thr Leu Pro Ser Glu Arg Glu Lys Ala Asp Glu 165 170 175 Ile Met Gly Thr Leu Leu Ser Arg Ser Ile Ala Gln Arg Thr Glu Asp 180 185 190 Asp Pro Asp Val Lys Pro Tyr Val Gln Glu Thr Ala Thr Glu Trp Ile 195 200 205 Lys Val Asp Leu Pro Pro Glu Met Lys Glu Ile Gln Arg Leu Leu Lys 210 215 220 Leu Ala Leu Asp Glu Arg Tyr Ser Ser Leu Lys Arg Cys Gly Tyr Asp 225 230 235 240 Leu Gly Ser Asn Arg Ser Leu Ser Ala Leu Leu Arg Leu Arg Met Val 245 250 255 Val Leu Gly Gly Asn Arg Arg Ala Ala Lys Pro Leu Phe Thr Ala Ile 260 265 270 Arg Ile Thr Tyr Ala Leu Asn Ile Phe Glu Ala His Gly Val Thr Pro 275 280 285 Phe Leu Lys Phe Cys Glu Arg Thr Ser Lys Lys Lys Gly Val Gly Val 290 295 300 Ala Glu Leu Phe Glu Gln Asp Arg Asn Phe Thr Gly Ala Ile Ala Arg 305 310 315 320 Ala Lys Ala Ala Gln Ala Ala Gly Met Glu His Pro Lys Ile Pro Lys 325 330 335 Leu Glu Asp Ala Val Arg Gly Ala Arg Gly Lys Ala Leu Val Phe Thr 340 345 350 Ser Tyr Arg Asp Ser Val Asp Leu Ile His Ser Arg Leu Lys Ala Ala 355 360 365 Gly Ile Asn Ser Gly Ile Leu Ile Gly Lys Ala Gly Glu Lys Gly Leu 370 375 380 Lys Gln Arg Lys Gln Val Glu Thr Val Ala Lys Phe Arg Asp Gly Gly 385 390 395 400 Tyr Asp Val Leu Val Ser Thr Arg Val Gly Glu Glu Gly Leu Asp Ile 405 410 415 Ser Glu Val Asn Leu Val Ile Phe Tyr Asp Asn Val Pro Ser Ser Ile 420 425 430 Arg Tyr Val Gln Arg Arg Gly Arg Thr Gly Arg Lys Asp Ala Gly Arg 435 440 445 Leu Ile Val Leu Met Ala Lys Gly Thr Ile Asp Glu Ala Tyr Tyr Trp 450 455 460 Ile Gly Arg Arg Lys Met Ser Ala Ala Lys Gly Met Gly Glu Arg Met 465 470 475 480 Asn Arg Ser Leu Ala Ala Gly Gly Ala Ala Ala Lys Ala Ala Pro Lys 485 490 495 Gly Leu Glu Gly Tyr Phe 500 <210> SEQ ID NO 35 <211> LENGTH: 402 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(402) <400> SEQUENCE: 35 gtg tca tcg tac ttt acc ata aag acc gcc aac ctg gcc ctg ccc gac 48 Met Ser Ser Tyr Phe Thr Ile Lys Thr Ala Asn Leu Ala Leu Pro Asp 1 5 10 15 gtg gtc aaa aag tac aac cac gtc ctg gca tgc aag agc gag gtg atg 96 Val Val Lys Lys Tyr Asn His Val Leu Ala Cys Lys Ser Glu Val Met 20 25 30 agg gcc gag aag cag atc cag acg tcc atc tcc tcg tct agc ggg ctc 144 Arg Ala Glu Lys Gln Ile Gln Thr Ser Ile Ser Ser Ser Ser Gly Leu 35 40 45 gac aag tac tcg gag ctc aag caa cag ttc aac tcc cgg ata acc gag 192 Asp Lys Tyr Ser Glu Leu Lys Gln Gln Phe Asn Ser Arg Ile Thr Glu 50 55 60 ttc tac cgc tcg ata gaa gag ctg gaa aag acc ggt gcg gtg gtc aag 240 Phe Tyr Arg Ser Ile Glu Glu Leu Glu Lys Thr Gly Ala Val Val Lys 65 70 75 80 agc ata gac gag ggc ctg ctg gac ttt ccc gca aag cgc ttt ggg gac 288 Ser Ile Asp Glu Gly Leu Leu Asp Phe Pro Ala Lys Arg Phe Gly Asp 85 90 95 gac atc tgg ctg tgc tgg aag aca ggc gag cgc gag atc aag ttc tgg 336 Asp Ile Trp Leu Cys Trp Lys Thr Gly Glu Arg Glu Ile Lys Phe Trp 100 105 110 cat gaa aag gac tct ggt ttt ggc gga aga aag ccc ata gag gta agt 384 His Glu Lys Asp Ser Gly Phe Gly Gly Arg Lys Pro Ile Glu Val Ser 115 120 125 gac gag tca cta gtg tag 402 Asp Glu Ser Leu Val 130 <210> SEQ ID NO 36 <211> LENGTH: 133 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 36 Met Ser Ser Tyr Phe Thr Ile Lys Thr Ala Asn Leu Ala Leu Pro Asp 1 5 10 15 Val Val Lys Lys Tyr Asn His Val Leu Ala Cys Lys Ser Glu Val Met 20 25 30 Arg Ala Glu Lys Gln Ile Gln Thr Ser Ile Ser Ser Ser Ser Gly Leu 35 40 45 Asp Lys Tyr Ser Glu Leu Lys Gln Gln Phe Asn Ser Arg Ile Thr Glu 50 55 60 Phe Tyr Arg Ser Ile Glu Glu Leu Glu Lys Thr Gly Ala Val Val Lys 65 70 75 80 Ser Ile Asp Glu Gly Leu Leu Asp Phe Pro Ala Lys Arg Phe Gly Asp 85 90 95 Asp Ile Trp Leu Cys Trp Lys Thr Gly Glu Arg Glu Ile Lys Phe Trp 100 105 110 His Glu Lys Asp Ser Gly Phe Gly Gly Arg Lys Pro Ile Glu Val Ser 115 120 125 Asp Glu Ser Leu Val 130 <210> SEQ ID NO 37 <211> LENGTH: 879 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(879) <400> SEQUENCE: 37 atg ctc tcc gcc tgg ttg cgc gta ata cgc gtc cgc ttc ctg ctc gcg 48 Met Leu Ser Ala Trp Leu Arg Val Ile Arg Val Arg Phe Leu Leu Ala 1 5 10 15 tcg gtg ata gcc gtc tcg gcg ggc ctc gcc ctc tcc tgg tgg cac ggc 96 Ser Val Ile Ala Val Ser Ala Gly Leu Ala Leu Ser Trp Trp His Gly 20 25 30 cac gaa ata gac gca ttc tcc gcc gcg ctc acc atg gcc ggc gtg gcc 144 His Glu Ile Asp Ala Phe Ser Ala Ala Leu Thr Met Ala Gly Val Ala 35 40 45 gcg ctc cac gca agc gtg gac atg ctc aac gat tat tcg gac tac aag 192 Ala Leu His Ala Ser Val Asp Met Leu Asn Asp Tyr Ser Asp Tyr Lys 50 55 60 cgc ggc ata gat acc ata acc aag agg acc ccg atg agc ggc gga aca 240 Arg Gly Ile Asp Thr Ile Thr Lys Arg Thr Pro Met Ser Gly Gly Thr 65 70 75 80 ggg gtg ctg cca gaa ggc ctg ctt acc ccc ggc cag gtg cac cgc gcc 288 Gly Val Leu Pro Glu Gly Leu Leu Thr Pro Gly Gln Val His Arg Ala 85 90 95 ggc atc ata tcg ctg gtc ctg ggc tct gct gtc ggc gcg tac ttt gtg 336 Gly Ile Ile Ser Leu Val Leu Gly Ser Ala Val Gly Ala Tyr Phe Val 100 105 110 gtc aca acg ggg ccc gtc ata gcc atg ata ctc ggc ttt gcc gta gtc 384 Val Thr Thr Gly Pro Val Ile Ala Met Ile Leu Gly Phe Ala Val Val 115 120 125 tcg ata tac ttt tac tcg acg agg att gta gac tcg ggc ctc tcc gag 432 Ser Ile Tyr Phe Tyr Ser Thr Arg Ile Val Asp Ser Gly Leu Ser Glu 130 135 140 gtc ttt gtg gcc gtc aag ggg gcg atg atc gtc ctt ggc gcc tac tac 480 Val Phe Val Ala Val Lys Gly Ala Met Ile Val Leu Gly Ala Tyr Tyr 145 150 155 160 ata cag gcg ccc gag ata acg cct gcc gcc gtt ctg gtg ggg gcg gcc 528 Ile Gln Ala Pro Glu Ile Thr Pro Ala Ala Val Leu Val Gly Ala Ala 165 170 175 gtg ggc gcc ctc tcg tcg gcg gtc ctc ttt gtg gcg tcg ttt cca gac 576 Val Gly Ala Leu Ser Ser Ala Val Leu Phe Val Ala Ser Phe Pro Asp 180 185 190 cac gat gcg gac aag tcc cgc ggc aga aag acg ctt gtt ata atc ctg 624 His Asp Ala Asp Lys Ser Arg Gly Arg Lys Thr Leu Val Ile Ile Leu 195 200 205 ggc aag gag agg gcc tcg cgg atc ctc tgg gtg ttc ccc gca gtg gca 672 Gly Lys Glu Arg Ala Ser Arg Ile Leu Trp Val Phe Pro Ala Val Ala 210 215 220 tac tcg tcc gtt ata acg ggg gtc atc ctg cag ttc ctg ccg gtg cat 720 Tyr Ser Ser Val Ile Thr Gly Val Ile Leu Gln Phe Leu Pro Val His 225 230 235 240 gca cta acc atg ctg ctt gca gcc ccc ctt gca gta att gcg gca aaa 768 Ala Leu Thr Met Leu Leu Ala Ala Pro Leu Ala Val Ile Ala Ala Lys 245 250 255 ggc ctt gcc agg gag tac ggc ggg gac ggg atc ata cgg gtc atg cgc 816 Gly Leu Ala Arg Glu Tyr Gly Gly Asp Gly Ile Ile Arg Val Met Arg 260 265 270 ggc acg ctg cgg ttt agc agg gtt gca ggc gcc ctg ctg gtg ttg ggc 864 Gly Thr Leu Arg Phe Ser Arg Val Ala Gly Ala Leu Leu Val Leu Gly 275 280 285 att ctg ttg ggc tga 879 Ile Leu Leu Gly 290 <210> SEQ ID NO 38 <211> LENGTH: 292 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 38 Met Leu Ser Ala Trp Leu Arg Val Ile Arg Val Arg Phe Leu Leu Ala 1 5 10 15 Ser Val Ile Ala Val Ser Ala Gly Leu Ala Leu Ser Trp Trp His Gly 20 25 30 His Glu Ile Asp Ala Phe Ser Ala Ala Leu Thr Met Ala Gly Val Ala 35 40 45 Ala Leu His Ala Ser Val Asp Met Leu Asn Asp Tyr Ser Asp Tyr Lys 50 55 60 Arg Gly Ile Asp Thr Ile Thr Lys Arg Thr Pro Met Ser Gly Gly Thr 65 70 75 80 Gly Val Leu Pro Glu Gly Leu Leu Thr Pro Gly Gln Val His Arg Ala 85 90 95 Gly Ile Ile Ser Leu Val Leu Gly Ser Ala Val Gly Ala Tyr Phe Val 100 105 110 Val Thr Thr Gly Pro Val Ile Ala Met Ile Leu Gly Phe Ala Val Val 115 120 125 Ser Ile Tyr Phe Tyr Ser Thr Arg Ile Val Asp Ser Gly Leu Ser Glu 130 135 140 Val Phe Val Ala Val Lys Gly Ala Met Ile Val Leu Gly Ala Tyr Tyr 145 150 155 160 Ile Gln Ala Pro Glu Ile Thr Pro Ala Ala Val Leu Val Gly Ala Ala 165 170 175 Val Gly Ala Leu Ser Ser Ala Val Leu Phe Val Ala Ser Phe Pro Asp 180 185 190 His Asp Ala Asp Lys Ser Arg Gly Arg Lys Thr Leu Val Ile Ile Leu 195 200 205 Gly Lys Glu Arg Ala Ser Arg Ile Leu Trp Val Phe Pro Ala Val Ala 210 215 220 Tyr Ser Ser Val Ile Thr Gly Val Ile Leu Gln Phe Leu Pro Val His 225 230 235 240 Ala Leu Thr Met Leu Leu Ala Ala Pro Leu Ala Val Ile Ala Ala Lys 245 250 255 Gly Leu Ala Arg Glu Tyr Gly Gly Asp Gly Ile Ile Arg Val Met Arg 260 265 270 Gly Thr Leu Arg Phe Ser Arg Val Ala Gly Ala Leu Leu Val Leu Gly 275 280 285 Ile Leu Leu Gly 290 <210> SEQ ID NO 39 <211> LENGTH: 1119 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1119) <400> SEQUENCE: 39 atg atc agc ggg cac gcc acg gcc gag ggt aca cgc agg ata gcc gag 48 Met Ile Ser Gly His Ala Thr Ala Glu Gly Thr Arg Arg Ile Ala Glu 1 5 10 15 atg tcg ggc gcc cat atc gac aac tac aag atg gtc gac ggg ctg cac 96 Met Ser Gly Ala His Ile Asp Asn Tyr Lys Met Val Asp Gly Leu His 20 25 30 ctc tcc aac gtg ggg atg ggc acc tac ctt ggc gac gcg gat gac gcc 144 Leu Ser Asn Val Gly Met Gly Thr Tyr Leu Gly Asp Ala Asp Asp Ala 35 40 45 acc gac agg gcc gtc acg gac gca gtc aag agg tcc gtc aaa aca ggc 192 Thr Asp Arg Ala Val Thr Asp Ala Val Lys Arg Ser Val Lys Thr Gly 50 55 60 ata aac gtc ata gat acg gcg ata aac tac cgc ctc cag agg gcc gag 240 Ile Asn Val Ile Asp Thr Ala Ile Asn Tyr Arg Leu Gln Arg Ala Glu 65 70 75 80 cgc tct gtc ggc agg gcc gtc acg gag ctc tca gaa gag ggg ctc gta 288 Arg Ser Val Gly Arg Ala Val Thr Glu Leu Ser Glu Glu Gly Leu Val 85 90 95 tca agg gac caa ata ttc ata tcg aca aag gcg ggc tat gta aca aac 336 Ser Arg Asp Gln Ile Phe Ile Ser Thr Lys Ala Gly Tyr Val Thr Asn 100 105 110 gac tcc gag gtc tcg ctt gac ttt tgg gag tat gtg aaa aaa gag tac 384 Asp Ser Glu Val Ser Leu Asp Phe Trp Glu Tyr Val Lys Lys Glu Tyr 115 120 125 gtc ggg ggc ggc gtg atc cag gca ggc gac ata tcc tcc gga tac cac 432 Val Gly Gly Gly Val Ile Gln Ala Gly Asp Ile Ser Ser Gly Tyr His 130 135 140 tgc atg aag ccc gcc tat cta gag gac cag ctg aag agg agc ctt gca 480 Cys Met Lys Pro Ala Tyr Leu Glu Asp Gln Leu Lys Arg Ser Leu Ala 145 150 155 160 aac atg ggc ctc gac tgt atc gac ctt gtc tac gtg cac aac ccc gtc 528 Asn Met Gly Leu Asp Cys Ile Asp Leu Val Tyr Val His Asn Pro Val 165 170 175 gag ggg cag atc aag gac cgc ccc ata ccg gag atc ctc gac tgt ata 576 Glu Gly Gln Ile Lys Asp Arg Pro Ile Pro Glu Ile Leu Asp Cys Ile 180 185 190 gga gag gcc ttt gcc atg tac gag aag gca agg gag gat ggc cgc atc 624 Gly Glu Ala Phe Ala Met Tyr Glu Lys Ala Arg Glu Asp Gly Arg Ile 195 200 205 aga tac tat ggg ctc gcc acg tgg gag tgc ttt cgt gtt gca ggg gac 672 Arg Tyr Tyr Gly Leu Ala Thr Trp Glu Cys Phe Arg Val Ala Gly Asp 210 215 220 aac ccg cag aat gtc cag ctc gaa gac gtt gta aag aag gcc aaa gac 720 Asn Pro Gln Asn Val Gln Leu Glu Asp Val Val Lys Lys Ala Lys Asp 225 230 235 240 gca ggc ggg gac aac cac gga ttc aag ttc ata cag ctg ccc ttc aac 768 Ala Gly Gly Asp Asn His Gly Phe Lys Phe Ile Gln Leu Pro Phe Asn 245 250 255 cag tac ttt gac cag gct tac atg cta aag aac cag acg gtg gac ggc 816 Gln Tyr Phe Asp Gln Ala Tyr Met Leu Lys Asn Gln Thr Val Asp Gly 260 265 270 aga aag ctg tcc ata ctg gat gcg gca gta tcc ctt ggc gtc ggt gtg 864 Arg Lys Leu Ser Ile Leu Asp Ala Ala Val Ser Leu Gly Val Gly Val 275 280 285 ttc acg agt gtc ccg ttc atg caa ggc aag ctg ctc gag cct ggc ctg 912 Phe Thr Ser Val Pro Phe Met Gln Gly Lys Leu Leu Glu Pro Gly Leu 290 295 300 ctg ccg gag ttt ggc ggg ctc tcc ccc gcc ctg cga tcc ctg cag ttt 960 Leu Pro Glu Phe Gly Gly Leu Ser Pro Ala Leu Arg Ser Leu Gln Phe 305 310 315 320 atc agg tct aca cca ggc gtg ctt gcc ccc ctg ccg ggg cac aac tca 1008 Ile Arg Ser Thr Pro Gly Val Leu Ala Pro Leu Pro Gly His Asn Ser 325 330 335 gct gcg cat aca gac gag aac ctc aag atc atg ggc gtg ccc ccc atc 1056 Ala Ala His Thr Asp Glu Asn Leu Lys Ile Met Gly Val Pro Pro Ile 340 345 350 ccg cct gac aag ttc ggg gag ctt gtg gcc agc ctc acc tcg tgg tcg 1104 Pro Pro Asp Lys Phe Gly Glu Leu Val Ala Ser Leu Thr Ser Trp Ser 355 360 365 ccc ggt cag aaa tag 1119 Pro Gly Gln Lys 370 <210> SEQ ID NO 40 <211> LENGTH: 372 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 40 Met Ile Ser Gly His Ala Thr Ala Glu Gly Thr Arg Arg Ile Ala Glu 1 5 10 15 Met Ser Gly Ala His Ile Asp Asn Tyr Lys Met Val Asp Gly Leu His 20 25 30 Leu Ser Asn Val Gly Met Gly Thr Tyr Leu Gly Asp Ala Asp Asp Ala 35 40 45 Thr Asp Arg Ala Val Thr Asp Ala Val Lys Arg Ser Val Lys Thr Gly 50 55 60 Ile Asn Val Ile Asp Thr Ala Ile Asn Tyr Arg Leu Gln Arg Ala Glu 65 70 75 80 Arg Ser Val Gly Arg Ala Val Thr Glu Leu Ser Glu Glu Gly Leu Val 85 90 95 Ser Arg Asp Gln Ile Phe Ile Ser Thr Lys Ala Gly Tyr Val Thr Asn 100 105 110 Asp Ser Glu Val Ser Leu Asp Phe Trp Glu Tyr Val Lys Lys Glu Tyr 115 120 125 Val Gly Gly Gly Val Ile Gln Ala Gly Asp Ile Ser Ser Gly Tyr His 130 135 140 Cys Met Lys Pro Ala Tyr Leu Glu Asp Gln Leu Lys Arg Ser Leu Ala 145 150 155 160 Asn Met Gly Leu Asp Cys Ile Asp Leu Val Tyr Val His Asn Pro Val 165 170 175 Glu Gly Gln Ile Lys Asp Arg Pro Ile Pro Glu Ile Leu Asp Cys Ile 180 185 190 Gly Glu Ala Phe Ala Met Tyr Glu Lys Ala Arg Glu Asp Gly Arg Ile 195 200 205 Arg Tyr Tyr Gly Leu Ala Thr Trp Glu Cys Phe Arg Val Ala Gly Asp 210 215 220 Asn Pro Gln Asn Val Gln Leu Glu Asp Val Val Lys Lys Ala Lys Asp 225 230 235 240 Ala Gly Gly Asp Asn His Gly Phe Lys Phe Ile Gln Leu Pro Phe Asn 245 250 255 Gln Tyr Phe Asp Gln Ala Tyr Met Leu Lys Asn Gln Thr Val Asp Gly 260 265 270 Arg Lys Leu Ser Ile Leu Asp Ala Ala Val Ser Leu Gly Val Gly Val 275 280 285 Phe Thr Ser Val Pro Phe Met Gln Gly Lys Leu Leu Glu Pro Gly Leu 290 295 300 Leu Pro Glu Phe Gly Gly Leu Ser Pro Ala Leu Arg Ser Leu Gln Phe 305 310 315 320 Ile Arg Ser Thr Pro Gly Val Leu Ala Pro Leu Pro Gly His Asn Ser 325 330 335 Ala Ala His Thr Asp Glu Asn Leu Lys Ile Met Gly Val Pro Pro Ile 340 345 350 Pro Pro Asp Lys Phe Gly Glu Leu Val Ala Ser Leu Thr Ser Trp Ser 355 360 365 Pro Gly Gln Lys 370 <210> SEQ ID NO 41 <211> LENGTH: 1107 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1107) <400> SEQUENCE: 41 atg gca cgg ggg cct atc ttg agt gaa aag ttc cag ata ctg cag ggc 48 Met Ala Arg Gly Pro Ile Leu Ser Glu Lys Phe Gln Ile Leu Gln Gly 1 5 10 15 gac gcc cgg gag gtg ctg ccg cgg ctg gca aag aat aca gcc gag cgc 96 Asp Ala Arg Glu Val Leu Pro Arg Leu Ala Lys Asn Thr Ala Glu Arg 20 25 30 ggc agg tac aga ctg gcg gta aca tcc cct ccc tat tac ggg cac aga 144 Gly Arg Tyr Arg Leu Ala Val Thr Ser Pro Pro Tyr Tyr Gly His Arg 35 40 45 aag tac ggg tcg gag ccc tcc gag ctg ggc cag gaa aag acg cca gac 192 Lys Tyr Gly Ser Glu Pro Ser Glu Leu Gly Gln Glu Lys Thr Pro Asp 50 55 60 gag ttc atc gag gag ctg gca gga gta ttc aag agc tgc atg gac ctg 240 Glu Phe Ile Glu Glu Leu Ala Gly Val Phe Lys Ser Cys Met Asp Leu 65 70 75 80 cta aca gac gac ggg agc ctc ttc ata gtg ata ggt gat acc agg agg 288 Leu Thr Asp Asp Gly Ser Leu Phe Ile Val Ile Gly Asp Thr Arg Arg 85 90 95 cgg cgc cac aag ctg atg gtc ccg cac cgg ctc gcg cta agg ctg gtg 336 Arg Arg His Lys Leu Met Val Pro His Arg Leu Ala Leu Arg Leu Val 100 105 110 gat ctt ggg tac cat ttc cag gag gat ata atc tgg tac aag cga aac 384 Asp Leu Gly Tyr His Phe Gln Glu Asp Ile Ile Trp Tyr Lys Arg Asn 115 120 125 gcc atc tcg caa agc tcg cgg caa aac ctg acg cag gcg tac gag ttt 432 Ala Ile Ser Gln Ser Ser Arg Gln Asn Leu Thr Gln Ala Tyr Glu Phe 130 135 140 gtt ctg gtc ctc tca aag tcg gat acc ccc gcc tat gac ata aac ccg 480 Val Leu Val Leu Ser Lys Ser Asp Thr Pro Ala Tyr Asp Ile Asn Pro 145 150 155 160 ata cgc gtc cag ggc aac gag gcc ctg agc ggg ata aac agc aaa ccc 528 Ile Arg Val Gln Gly Asn Glu Ala Leu Ser Gly Ile Asn Ser Lys Pro 165 170 175 gca aat gac cgg ctg cag ttc gcc ccc ggg aag agg gat ccc gag gca 576 Ala Asn Asp Arg Leu Gln Phe Ala Pro Gly Lys Arg Asp Pro Glu Ala 180 185 190 ata ggg agg att gca gcc gtg ata cac ggc tca acg cct ggt acg ccg 624 Ile Gly Arg Ile Ala Ala Val Ile His Gly Ser Thr Pro Gly Thr Pro 195 200 205 ttt gac gag ctg cca acc acc ggg gaa ata tca tgg gcc cac ggc tat 672 Phe Asp Glu Leu Pro Thr Thr Gly Glu Ile Ser Trp Ala His Gly Tyr 210 215 220 gac ccc gaa aag tac tgc ccc acg tgc tat cgc aag ttc cgg agg cat 720 Asp Pro Glu Lys Tyr Cys Pro Thr Cys Tyr Arg Lys Phe Arg Arg His 225 230 235 240 gcg acg cgc aag agg ata ggg ggc cac gag cac tat ccg ata ttt gcc 768 Ala Thr Arg Lys Arg Ile Gly Gly His Glu His Tyr Pro Ile Phe Ala 245 250 255 gca tgc aac ccg cgg ggc aag aac ccg ggg aac gtc tgg gag ata tcc 816 Ala Cys Asn Pro Arg Gly Lys Asn Pro Gly Asn Val Trp Glu Ile Ser 260 265 270 aca aag gcg cac cat gga aac gag cac ttt gcg gta ttc cca gaa gac 864 Thr Lys Ala His His Gly Asn Glu His Phe Ala Val Phe Pro Glu Asp 275 280 285 ctt gta tcc agg ata gta aag ttt gcc aca aaa gag ggc gat tac gtg 912 Leu Val Ser Arg Ile Val Lys Phe Ala Thr Lys Glu Gly Asp Tyr Val 290 295 300 ctg gac ccg ttt gca ggc agg ggg acc acg gga ata gtc tct gca tgc 960 Leu Asp Pro Phe Ala Gly Arg Gly Thr Thr Gly Ile Val Ser Ala Cys 305 310 315 320 ctc aag agg ggc ttt acc ggg ata gac ctg tat cct gcc aac gtg gca 1008 Leu Lys Arg Gly Phe Thr Gly Ile Asp Leu Tyr Pro Ala Asn Val Ala 325 330 335 agg gcc cgg cgc aac gtg cag gat tcc gcc gat tca cgg ctc tca aaa 1056 Arg Ala Arg Arg Asn Val Gln Asp Ser Ala Asp Ser Arg Leu Ser Lys 340 345 350 aag gtg ctc gac cag ata atg ccc gag agg cag ctg acc ggc tat ttc 1104 Lys Val Leu Asp Gln Ile Met Pro Glu Arg Gln Leu Thr Gly Tyr Phe 355 360 365 tga 1107 <210> SEQ ID NO 42 <211> LENGTH: 368 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 42 Met Ala Arg Gly Pro Ile Leu Ser Glu Lys Phe Gln Ile Leu Gln Gly 1 5 10 15 Asp Ala Arg Glu Val Leu Pro Arg Leu Ala Lys Asn Thr Ala Glu Arg 20 25 30 Gly Arg Tyr Arg Leu Ala Val Thr Ser Pro Pro Tyr Tyr Gly His Arg 35 40 45 Lys Tyr Gly Ser Glu Pro Ser Glu Leu Gly Gln Glu Lys Thr Pro Asp 50 55 60 Glu Phe Ile Glu Glu Leu Ala Gly Val Phe Lys Ser Cys Met Asp Leu 65 70 75 80 Leu Thr Asp Asp Gly Ser Leu Phe Ile Val Ile Gly Asp Thr Arg Arg 85 90 95 Arg Arg His Lys Leu Met Val Pro His Arg Leu Ala Leu Arg Leu Val 100 105 110 Asp Leu Gly Tyr His Phe Gln Glu Asp Ile Ile Trp Tyr Lys Arg Asn 115 120 125 Ala Ile Ser Gln Ser Ser Arg Gln Asn Leu Thr Gln Ala Tyr Glu Phe 130 135 140 Val Leu Val Leu Ser Lys Ser Asp Thr Pro Ala Tyr Asp Ile Asn Pro 145 150 155 160 Ile Arg Val Gln Gly Asn Glu Ala Leu Ser Gly Ile Asn Ser Lys Pro 165 170 175 Ala Asn Asp Arg Leu Gln Phe Ala Pro Gly Lys Arg Asp Pro Glu Ala 180 185 190 Ile Gly Arg Ile Ala Ala Val Ile His Gly Ser Thr Pro Gly Thr Pro 195 200 205 Phe Asp Glu Leu Pro Thr Thr Gly Glu Ile Ser Trp Ala His Gly Tyr 210 215 220 Asp Pro Glu Lys Tyr Cys Pro Thr Cys Tyr Arg Lys Phe Arg Arg His 225 230 235 240 Ala Thr Arg Lys Arg Ile Gly Gly His Glu His Tyr Pro Ile Phe Ala 245 250 255 Ala Cys Asn Pro Arg Gly Lys Asn Pro Gly Asn Val Trp Glu Ile Ser 260 265 270 Thr Lys Ala His His Gly Asn Glu His Phe Ala Val Phe Pro Glu Asp 275 280 285 Leu Val Ser Arg Ile Val Lys Phe Ala Thr Lys Glu Gly Asp Tyr Val 290 295 300 Leu Asp Pro Phe Ala Gly Arg Gly Thr Thr Gly Ile Val Ser Ala Cys 305 310 315 320 Leu Lys Arg Gly Phe Thr Gly Ile Asp Leu Tyr Pro Ala Asn Val Ala 325 330 335 Arg Ala Arg Arg Asn Val Gln Asp Ser Ala Asp Ser Arg Leu Ser Lys 340 345 350 Lys Val Leu Asp Gln Ile Met Pro Glu Arg Gln Leu Thr Gly Tyr Phe 355 360 365 <210> SEQ ID NO 43 <211> LENGTH: 933 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(933) <400> SEQUENCE: 43 atg cct agt tac gca gaa ata gca aac gac gta ctt cga cta atg gag 48 Met Pro Ser Tyr Ala Glu Ile Ala Asn Asp Val Leu Arg Leu Met Glu 1 5 10 15 tca gtc ggt gag cag gca cct ggt gta gta ctt cac gac tat ctt tca 96 Ser Val Gly Glu Gln Ala Pro Gly Val Val Leu His Asp Tyr Leu Ser 20 25 30 aaa ttg caa cag tat tcg ggg agg gat aca ata ctg tat gcg acc aac 144 Lys Leu Gln Gln Tyr Ser Gly Arg Asp Thr Ile Leu Tyr Ala Thr Asn 35 40 45 tgg ata acg gac gaa gcg cat acg tct aat gaa gct ctc ata aca aat 192 Trp Ile Thr Asp Glu Ala His Thr Ser Asn Glu Ala Leu Ile Thr Asn 50 55 60 ggt gac ctg tat gga ttt atg agg atg atg cgt gat tta aag act aag 240 Gly Asp Leu Tyr Gly Phe Met Arg Met Met Arg Asp Leu Lys Thr Lys 65 70 75 80 aaa tta gat tta ata ctc cac agt ccg ggg ggc tcc gtc gag tcc acc 288 Lys Leu Asp Leu Ile Leu His Ser Pro Gly Gly Ser Val Glu Ser Thr 85 90 95 gaa gca atc gtc tca tac ata cgt gca aaa ttt aaa aat gtc cgg atc 336 Glu Ala Ile Val Ser Tyr Ile Arg Ala Lys Phe Lys Asn Val Arg Ile 100 105 110 att atc cca tat gcc gcg atg tcg gca gct gcg atg ctt gca tgc tca 384 Ile Ile Pro Tyr Ala Ala Met Ser Ala Ala Ala Met Leu Ala Cys Ser 115 120 125 tcg aat tgc ctg gta atg ggt aaa cac tca tcg ata ggt ccc acc gac 432 Ser Asn Cys Leu Val Met Gly Lys His Ser Ser Ile Gly Pro Thr Asp 130 135 140 ccc caa ttt att att cca acc agg acc ggc atg cac ata atg tct gca 480 Pro Gln Phe Ile Ile Pro Thr Arg Thr Gly Met His Ile Met Ser Ala 145 150 155 160 cag ttt cta att agc gag ttt caa gaa gca cag tcg gtg tca gaa aaa 528 Gln Phe Leu Ile Ser Glu Phe Gln Glu Ala Gln Ser Val Ser Glu Lys 165 170 175 cac ccg ggg agg ctc ggc gca tgg ctt cca ctg tta ggg caa tat cct 576 His Pro Gly Arg Leu Gly Ala Trp Leu Pro Leu Leu Gly Gln Tyr Pro 180 185 190 ccc ggg cta att caa aaa tgc att agc agc cag aag cta agt gtg gaa 624 Pro Gly Leu Ile Gln Lys Cys Ile Ser Ser Gln Lys Leu Ser Val Glu 195 200 205 ctt gta caa aaa tgg ctg gct aga tac atg ttt gag aac gag tct gca 672 Leu Val Gln Lys Trp Leu Ala Arg Tyr Met Phe Glu Asn Glu Ser Ala 210 215 220 gcg gta aaa aag tca aaa aaa ata tca gaa ata atg tct tcc tct aaa 720 Ala Val Lys Lys Ser Lys Lys Ile Ser Glu Ile Met Ser Ser Ser Lys 225 230 235 240 aaa tat cac agt cat gga agg cgc ata tcg aga gaa gaa tgt aaa agg 768 Lys Tyr His Ser His Gly Arg Arg Ile Ser Arg Glu Glu Cys Lys Arg 245 250 255 att ggc tta aaa gta act gat ctg gaa gat gaa caa gaa ttt caa gat 816 Ile Gly Leu Lys Val Thr Asp Leu Glu Asp Glu Gln Glu Phe Gln Asp 260 265 270 ctg gtg ctg tca gta ttt cat gcg gca aat acc atg ttt cag tat act 864 Leu Val Leu Ser Val Phe His Ala Ala Asn Thr Met Phe Gln Tyr Thr 275 280 285 cca gtc aac aaa att atc atg aat cac ctc ggt aat acc gtc gtt gag 912 Pro Val Asn Lys Ile Ile Met Asn His Leu Gly Asn Thr Val Val Glu 290 295 300 aca ctg cca aca cca cgg taa 933 Thr Leu Pro Thr Pro Arg 305 310 <210> SEQ ID NO 44 <211> LENGTH: 310 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 44 Met Pro Ser Tyr Ala Glu Ile Ala Asn Asp Val Leu Arg Leu Met Glu 1 5 10 15 Ser Val Gly Glu Gln Ala Pro Gly Val Val Leu His Asp Tyr Leu Ser 20 25 30 Lys Leu Gln Gln Tyr Ser Gly Arg Asp Thr Ile Leu Tyr Ala Thr Asn 35 40 45 Trp Ile Thr Asp Glu Ala His Thr Ser Asn Glu Ala Leu Ile Thr Asn 50 55 60 Gly Asp Leu Tyr Gly Phe Met Arg Met Met Arg Asp Leu Lys Thr Lys 65 70 75 80 Lys Leu Asp Leu Ile Leu His Ser Pro Gly Gly Ser Val Glu Ser Thr 85 90 95 Glu Ala Ile Val Ser Tyr Ile Arg Ala Lys Phe Lys Asn Val Arg Ile 100 105 110 Ile Ile Pro Tyr Ala Ala Met Ser Ala Ala Ala Met Leu Ala Cys Ser 115 120 125 Ser Asn Cys Leu Val Met Gly Lys His Ser Ser Ile Gly Pro Thr Asp 130 135 140 Pro Gln Phe Ile Ile Pro Thr Arg Thr Gly Met His Ile Met Ser Ala 145 150 155 160 Gln Phe Leu Ile Ser Glu Phe Gln Glu Ala Gln Ser Val Ser Glu Lys 165 170 175 His Pro Gly Arg Leu Gly Ala Trp Leu Pro Leu Leu Gly Gln Tyr Pro 180 185 190 Pro Gly Leu Ile Gln Lys Cys Ile Ser Ser Gln Lys Leu Ser Val Glu 195 200 205 Leu Val Gln Lys Trp Leu Ala Arg Tyr Met Phe Glu Asn Glu Ser Ala 210 215 220 Ala Val Lys Lys Ser Lys Lys Ile Ser Glu Ile Met Ser Ser Ser Lys 225 230 235 240 Lys Tyr His Ser His Gly Arg Arg Ile Ser Arg Glu Glu Cys Lys Arg 245 250 255 Ile Gly Leu Lys Val Thr Asp Leu Glu Asp Glu Gln Glu Phe Gln Asp 260 265 270 Leu Val Leu Ser Val Phe His Ala Ala Asn Thr Met Phe Gln Tyr Thr 275 280 285 Pro Val Asn Lys Ile Ile Met Asn His Leu Gly Asn Thr Val Val Glu 290 295 300 Thr Leu Pro Thr Pro Arg 305 310 <210> SEQ ID NO 45 <211> LENGTH: 1305 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1305) <400> SEQUENCE: 45 gtg gat ctg gaa cgc gag tac agg gca aag acc ggc ggc tcg gcc cgg 48 Met Asp Leu Glu Arg Glu Tyr Arg Ala Lys Thr Gly Gly Ser Ala Arg 1 5 10 15 atc ttt gcc agg tcg aaa aag tac cac gtc ggc ggg gtc agc cac aac 96 Ile Phe Ala Arg Ser Lys Lys Tyr His Val Gly Gly Val Ser His Asn 20 25 30 ata agg ttc tac gag ccg tat ccg ttt gtg aca agg tcc gcg agc ggc 144 Ile Arg Phe Tyr Glu Pro Tyr Pro Phe Val Thr Arg Ser Ala Ser Gly 35 40 45 aag cac ctc gtc gac gtg gac ggg aac aag tat gta gac tac tgg atg 192 Lys His Leu Val Asp Val Asp Gly Asn Lys Tyr Val Asp Tyr Trp Met 50 55 60 ggg cac tgg agc ctg ata ctg ggg cac gcg ccg gcg cca gtc agg tcg 240 Gly His Trp Ser Leu Ile Leu Gly His Ala Pro Ala Pro Val Arg Ser 65 70 75 80 gca gta gag ggg cag ctt cgc cgc ggc tgg atc cac ggg acc gtc aac 288 Ala Val Glu Gly Gln Leu Arg Arg Gly Trp Ile His Gly Thr Val Asn 85 90 95 gag cag acg atg aat ctc tcg gag ata ata cgc ggc gcg gta agc gtg 336 Glu Gln Thr Met Asn Leu Ser Glu Ile Ile Arg Gly Ala Val Ser Val 100 105 110 gca gaa aag aca agg tac gtc acg tcg ggg acg gag gcc gtc atg tat 384 Ala Glu Lys Thr Arg Tyr Val Thr Ser Gly Thr Glu Ala Val Met Tyr 115 120 125 gcg gca agg ctg gcg cgc gcg cat acg ggc aga aaa ata ata gca aag 432 Ala Ala Arg Leu Ala Arg Ala His Thr Gly Arg Lys Ile Ile Ala Lys 130 135 140 gcg gac ggc ggc tgg cac ggg tac gcg tcg ggg ctg ctc aag tcg gtc 480 Ala Asp Gly Gly Trp His Gly Tyr Ala Ser Gly Leu Leu Lys Ser Val 145 150 155 160 aac tgg ccg tat gat gtg ccc gag agc ggg ggg ctc gtc gac gaa gag 528 Asn Trp Pro Tyr Asp Val Pro Glu Ser Gly Gly Leu Val Asp Glu Glu 165 170 175 cac tct ata tcc att ccg tac aac gat ctt gaa ggt tcc ctg gat gtt 576 His Ser Ile Ser Ile Pro Tyr Asn Asp Leu Glu Gly Ser Leu Asp Val 180 185 190 ctt ggg cgc gca ggc gac gac ttg gca tgc gtg ata atc gag ccg ctg 624 Leu Gly Arg Ala Gly Asp Asp Leu Ala Cys Val Ile Ile Glu Pro Leu 195 200 205 ctg ggc ggc ggc ggc tgc ata ccg gcg gat gag gac tat ctg cgc ggc 672 Leu Gly Gly Gly Gly Cys Ile Pro Ala Asp Glu Asp Tyr Leu Arg Gly 210 215 220 ata cag gag ttt gtg cat tca agg ggc gcg ctg ctt gtc ctc gac gag 720 Ile Gln Glu Phe Val His Ser Arg Gly Ala Leu Leu Val Leu Asp Glu 225 230 235 240 ata gtg aca ggg ttc cgg ttt agg ttt ggc tgc gcg tat gct gca gca 768 Ile Val Thr Gly Phe Arg Phe Arg Phe Gly Cys Ala Tyr Ala Ala Ala 245 250 255 ggg ctg gac ccc gat ata gtg gcg ctc ggc aag ata gtc ggg ggc gga 816 Gly Leu Asp Pro Asp Ile Val Ala Leu Gly Lys Ile Val Gly Gly Gly 260 265 270 ttc ccc ata ggg gtg ata tgc ggc aag gac gag gtg atg gaa atc tcc 864 Phe Pro Ile Gly Val Ile Cys Gly Lys Asp Glu Val Met Glu Ile Ser 275 280 285 aac act ata tcg cat gca aag tcc gac agg gcg tac atc ggc ggc ggc 912 Asn Thr Ile Ser His Ala Lys Ser Asp Arg Ala Tyr Ile Gly Gly Gly 290 295 300 aca ttc tct gca aac ccc gcc acg atg aca gcg ggc gcg gca gcg ctc 960 Thr Phe Ser Ala Asn Pro Ala Thr Met Thr Ala Gly Ala Ala Ala Leu 305 310 315 320 ggg gag ctc aaa aag aga aag ggc aca ata tac ccg agg ata aac tcc 1008 Gly Glu Leu Lys Lys Arg Lys Gly Thr Ile Tyr Pro Arg Ile Asn Ser 325 330 335 atg ggg gac gac gca agg gac aag ctc tca aag ata ttt ggg aac agg 1056 Met Gly Asp Asp Ala Arg Asp Lys Leu Ser Lys Ile Phe Gly Asn Arg 340 345 350 gta tcc gtg acc gga agg ggc tcg ctg ttc atg act cac ttt gtt caa 1104 Val Ser Val Thr Gly Arg Gly Ser Leu Phe Met Thr His Phe Val Gln 355 360 365 gat ggc gcc ggc agg gtc tca aat gct gca gat gcg gca gcc tgc gat 1152 Asp Gly Ala Gly Arg Val Ser Asn Ala Ala Asp Ala Ala Ala Cys Asp 370 375 380 gtt gag ctg ctg cac agg tac cac ctg gac atg atc acc cgg gac ggc 1200 Val Glu Leu Leu His Arg Tyr His Leu Asp Met Ile Thr Arg Asp Gly 385 390 395 400 ata ttc ttt ctg ccg ggc aag ctg ggg gcc ata tcg gcg gcg cac tca 1248 Ile Phe Phe Leu Pro Gly Lys Leu Gly Ala Ile Ser Ala Ala His Ser 405 410 415 aag gcc gac ctc aag acc atg tat tcc gca tca gag cgc ttt gca gaa 1296 Lys Ala Asp Leu Lys Thr Met Tyr Ser Ala Ser Glu Arg Phe Ala Glu 420 425 430 ggc cta tga 1305 Gly Leu <210> SEQ ID NO 46 <211> LENGTH: 434 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 46 Met Asp Leu Glu Arg Glu Tyr Arg Ala Lys Thr Gly Gly Ser Ala Arg 1 5 10 15 Ile Phe Ala Arg Ser Lys Lys Tyr His Val Gly Gly Val Ser His Asn 20 25 30 Ile Arg Phe Tyr Glu Pro Tyr Pro Phe Val Thr Arg Ser Ala Ser Gly 35 40 45 Lys His Leu Val Asp Val Asp Gly Asn Lys Tyr Val Asp Tyr Trp Met 50 55 60 Gly His Trp Ser Leu Ile Leu Gly His Ala Pro Ala Pro Val Arg Ser 65 70 75 80 Ala Val Glu Gly Gln Leu Arg Arg Gly Trp Ile His Gly Thr Val Asn 85 90 95 Glu Gln Thr Met Asn Leu Ser Glu Ile Ile Arg Gly Ala Val Ser Val 100 105 110 Ala Glu Lys Thr Arg Tyr Val Thr Ser Gly Thr Glu Ala Val Met Tyr 115 120 125 Ala Ala Arg Leu Ala Arg Ala His Thr Gly Arg Lys Ile Ile Ala Lys 130 135 140 Ala Asp Gly Gly Trp His Gly Tyr Ala Ser Gly Leu Leu Lys Ser Val 145 150 155 160 Asn Trp Pro Tyr Asp Val Pro Glu Ser Gly Gly Leu Val Asp Glu Glu 165 170 175 His Ser Ile Ser Ile Pro Tyr Asn Asp Leu Glu Gly Ser Leu Asp Val 180 185 190 Leu Gly Arg Ala Gly Asp Asp Leu Ala Cys Val Ile Ile Glu Pro Leu 195 200 205 Leu Gly Gly Gly Gly Cys Ile Pro Ala Asp Glu Asp Tyr Leu Arg Gly 210 215 220 Ile Gln Glu Phe Val His Ser Arg Gly Ala Leu Leu Val Leu Asp Glu 225 230 235 240 Ile Val Thr Gly Phe Arg Phe Arg Phe Gly Cys Ala Tyr Ala Ala Ala 245 250 255 Gly Leu Asp Pro Asp Ile Val Ala Leu Gly Lys Ile Val Gly Gly Gly 260 265 270 Phe Pro Ile Gly Val Ile Cys Gly Lys Asp Glu Val Met Glu Ile Ser 275 280 285 Asn Thr Ile Ser His Ala Lys Ser Asp Arg Ala Tyr Ile Gly Gly Gly 290 295 300 Thr Phe Ser Ala Asn Pro Ala Thr Met Thr Ala Gly Ala Ala Ala Leu 305 310 315 320 Gly Glu Leu Lys Lys Arg Lys Gly Thr Ile Tyr Pro Arg Ile Asn Ser 325 330 335 Met Gly Asp Asp Ala Arg Asp Lys Leu Ser Lys Ile Phe Gly Asn Arg 340 345 350 Val Ser Val Thr Gly Arg Gly Ser Leu Phe Met Thr His Phe Val Gln 355 360 365 Asp Gly Ala Gly Arg Val Ser Asn Ala Ala Asp Ala Ala Ala Cys Asp 370 375 380 Val Glu Leu Leu His Arg Tyr His Leu Asp Met Ile Thr Arg Asp Gly 385 390 395 400 Ile Phe Phe Leu Pro Gly Lys Leu Gly Ala Ile Ser Ala Ala His Ser 405 410 415 Lys Ala Asp Leu Lys Thr Met Tyr Ser Ala Ser Glu Arg Phe Ala Glu 420 425 430 Gly Leu <210> SEQ ID NO 47 <211> LENGTH: 807 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(807) <400> SEQUENCE: 47 atg ata ctc ttc ggc aag agc gac ccc gcc gag ctg gtg cgc cag gcg 48 Met Ile Leu Phe Gly Lys Ser Asp Pro Ala Glu Leu Val Arg Gln Ala 1 5 10 15 gac ctc ctg tgc agc aag aac cag ttc agg gcg gca ata ggc ctg tac 96 Asp Leu Leu Cys Ser Lys Asn Gln Phe Arg Ala Ala Ile Gly Leu Tyr 20 25 30 ggg aaa atc ctc aag gac gac ccg cag aac agg ggc gtc ctg cac aaa 144 Gly Lys Ile Leu Lys Asp Asp Pro Gln Asn Arg Gly Val Leu His Lys 35 40 45 aag ggg ctg gcc cag aac agg gca aaa aag tac tct gat gcg atc acg 192 Lys Gly Leu Ala Gln Asn Arg Ala Lys Lys Tyr Ser Asp Ala Ile Thr 50 55 60 tgc ttt gac cgg ctg ctc gag ctt gac aac aag gac gcg ccc gcg tac 240 Cys Phe Asp Arg Leu Leu Glu Leu Asp Asn Lys Asp Ala Pro Ala Tyr 65 70 75 80 aac aac aag gcc ata gcc cag gcc gag ctc gga gac acg gca tcc gcg 288 Asn Asn Lys Ala Ile Ala Gln Ala Glu Leu Gly Asp Thr Ala Ser Ala 85 90 95 ctg gaa aac tac ggc agg gcc atc gag gcc gac ccg cgg tac gcg ccg 336 Leu Glu Asn Tyr Gly Arg Ala Ile Glu Ala Asp Pro Arg Tyr Ala Pro 100 105 110 gcg cgc ttc aac agg gcc gtg ctg ctc gac agg ctg ggc gag cat gag 384 Ala Arg Phe Asn Arg Ala Val Leu Leu Asp Arg Leu Gly Glu His Glu 115 120 125 gag gcg ctg ccg gac ctc gac agg gca gcc gag ctg gac cga cgc aag 432 Glu Ala Leu Pro Asp Leu Asp Arg Ala Ala Glu Leu Asp Arg Arg Lys 130 135 140 ccg aac ccg agg ttc tac aag ggg ata gtg ctc ggc aag atg ggc agg 480 Pro Asn Pro Arg Phe Tyr Lys Gly Ile Val Leu Gly Lys Met Gly Arg 145 150 155 160 cac gaa gag gcg ctg gcc tgc ttc aag ggc gtg tgc aag agg cat ccc 528 His Glu Glu Ala Leu Ala Cys Phe Lys Gly Val Cys Lys Arg His Pro 165 170 175 ggc cac gcc gac tca cag ttc cac gtg ggg ata gag ctt acc gag ctt 576 Gly His Ala Asp Ser Gln Phe His Val Gly Ile Glu Leu Thr Glu Leu 180 185 190 ggc agg cac gcc gag gcc ctc ggg gag ctt gca tca ctg ccc gcg gag 624 Gly Arg His Ala Glu Ala Leu Gly Glu Leu Ala Ser Leu Pro Ala Glu 195 200 205 cac cgc gag aac gcc aat gta ttg tat gcc agg gcg cgc agc ctc tcg 672 His Arg Glu Asn Ala Asn Val Leu Tyr Ala Arg Ala Arg Ser Leu Ser 210 215 220 ggc ctt ggc agg gag gac gaa tcc ata gcg cac ctg caa aag gcg gcc 720 Gly Leu Gly Arg Glu Asp Glu Ser Ile Ala His Leu Gln Lys Ala Ala 225 230 235 240 aaa aaa gat tcc aag acg ata aaa aag tgg gcc cgc gca gaa aag gcc 768 Lys Lys Asp Ser Lys Thr Ile Lys Lys Trp Ala Arg Ala Glu Lys Ala 245 250 255 ttt gac gga ata cgg gac gat ccc ggt tca aaa aga tag 807 Phe Asp Gly Ile Arg Asp Asp Pro Gly Ser Lys Arg 260 265 <210> SEQ ID NO 48 <211> LENGTH: 268 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 48 Met Ile Leu Phe Gly Lys Ser Asp Pro Ala Glu Leu Val Arg Gln Ala 1 5 10 15 Asp Leu Leu Cys Ser Lys Asn Gln Phe Arg Ala Ala Ile Gly Leu Tyr 20 25 30 Gly Lys Ile Leu Lys Asp Asp Pro Gln Asn Arg Gly Val Leu His Lys 35 40 45 Lys Gly Leu Ala Gln Asn Arg Ala Lys Lys Tyr Ser Asp Ala Ile Thr 50 55 60 Cys Phe Asp Arg Leu Leu Glu Leu Asp Asn Lys Asp Ala Pro Ala Tyr 65 70 75 80 Asn Asn Lys Ala Ile Ala Gln Ala Glu Leu Gly Asp Thr Ala Ser Ala 85 90 95 Leu Glu Asn Tyr Gly Arg Ala Ile Glu Ala Asp Pro Arg Tyr Ala Pro 100 105 110 Ala Arg Phe Asn Arg Ala Val Leu Leu Asp Arg Leu Gly Glu His Glu 115 120 125 Glu Ala Leu Pro Asp Leu Asp Arg Ala Ala Glu Leu Asp Arg Arg Lys 130 135 140 Pro Asn Pro Arg Phe Tyr Lys Gly Ile Val Leu Gly Lys Met Gly Arg 145 150 155 160 His Glu Glu Ala Leu Ala Cys Phe Lys Gly Val Cys Lys Arg His Pro 165 170 175 Gly His Ala Asp Ser Gln Phe His Val Gly Ile Glu Leu Thr Glu Leu 180 185 190 Gly Arg His Ala Glu Ala Leu Gly Glu Leu Ala Ser Leu Pro Ala Glu 195 200 205 His Arg Glu Asn Ala Asn Val Leu Tyr Ala Arg Ala Arg Ser Leu Ser 210 215 220 Gly Leu Gly Arg Glu Asp Glu Ser Ile Ala His Leu Gln Lys Ala Ala 225 230 235 240 Lys Lys Asp Ser Lys Thr Ile Lys Lys Trp Ala Arg Ala Glu Lys Ala 245 250 255 Phe Asp Gly Ile Arg Asp Asp Pro Gly Ser Lys Arg 260 265 <210> SEQ ID NO 49 <211> LENGTH: 708 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(708) <400> SEQUENCE: 49 gtg cgg cag ggg atg act gga aag acc agg acg gcg gtc ctg cgg aac 48 Met Arg Gln Gly Met Thr Gly Lys Thr Arg Thr Ala Val Leu Arg Asn 1 5 10 15 gcc atg act gag gag tcg gct cgg gcc atg ata gag gca aag aag acg 96 Ala Met Thr Glu Glu Ser Ala Arg Ala Met Ile Glu Ala Lys Lys Thr 20 25 30 ggt gcc ttt agg gcc ctt atg agg gcc ccg cgg aaa gaa gac gtc cat 144 Gly Ala Phe Arg Ala Leu Met Arg Ala Pro Arg Lys Glu Asp Val His 35 40 45 gtg cat tct gta aag ctg gtc cac gag gcg ctg atc cgg gtc tcc gcc 192 Val His Ser Val Lys Leu Val His Glu Ala Leu Ile Arg Val Ser Ala 50 55 60 agg tac tct gcg gat ttt ttc aga aag gcg gtt cac ccg atc aag gtg 240 Arg Tyr Ser Ala Asp Phe Phe Arg Lys Ala Val His Pro Ile Lys Val 65 70 75 80 gac cag aac gtg atc gag gtg gtg cta ggc gac ggc gtc ttt ccc ata 288 Asp Gln Asn Val Ile Glu Val Val Leu Gly Asp Gly Val Phe Pro Ile 85 90 95 agg tcc aag tcg cgc ata cac aag acg ctc tcg gca ggg ctc ggc aag 336 Arg Ser Lys Ser Arg Ile His Lys Thr Leu Ser Ala Gly Leu Gly Lys 100 105 110 aac agg gtc gac ctc gag cta gaa gag cat gtc ttt gcg gaa tca gaa 384 Asn Arg Val Asp Leu Glu Leu Glu Glu His Val Phe Ala Glu Ser Glu 115 120 125 ggg atg atg tgc ctt gac cgg cac ggc ggc gag acg gac ttt ccc tac 432 Gly Met Met Cys Leu Asp Arg His Gly Gly Glu Thr Asp Phe Pro Tyr 130 135 140 aag acg ggg ccc ggc gcg gtg gag ccg tac ccg cgg agg ata ctc gat 480 Lys Thr Gly Pro Gly Ala Val Glu Pro Tyr Pro Arg Arg Ile Leu Asp 145 150 155 160 gcg tca gag aat gtg cgg agc ccc gag gtg gag aca gaa gag gcg ctc 528 Ala Ser Glu Asn Val Arg Ser Pro Glu Val Glu Thr Glu Glu Ala Leu 165 170 175 tca aaa cta aaa gag aag ctg cgc ggg ccc ccg cct gac ggc atg cgc 576 Ser Lys Leu Lys Glu Lys Leu Arg Gly Pro Pro Pro Asp Gly Met Arg 180 185 190 gac ctg cgg gag gag ttt gcc gca aag gcg gtg gag gtg gtc tat gta 624 Asp Leu Arg Glu Glu Phe Ala Ala Lys Ala Val Glu Val Val Tyr Val 195 200 205 cca gtc tat gaa tcg cga ctt gtg ggg ccc aaa aaa aag gtc cgc atg 672 Pro Val Tyr Glu Ser Arg Leu Val Gly Pro Lys Lys Lys Val Arg Met 210 215 220 atg cgg att gac gcg gca aga aaa aag atc ctc tag 708 Met Arg Ile Asp Ala Ala Arg Lys Lys Ile Leu 225 230 235 <210> SEQ ID NO 50 <211> LENGTH: 235 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 50 Met Arg Gln Gly Met Thr Gly Lys Thr Arg Thr Ala Val Leu Arg Asn 1 5 10 15 Ala Met Thr Glu Glu Ser Ala Arg Ala Met Ile Glu Ala Lys Lys Thr 20 25 30 Gly Ala Phe Arg Ala Leu Met Arg Ala Pro Arg Lys Glu Asp Val His 35 40 45 Val His Ser Val Lys Leu Val His Glu Ala Leu Ile Arg Val Ser Ala 50 55 60 Arg Tyr Ser Ala Asp Phe Phe Arg Lys Ala Val His Pro Ile Lys Val 65 70 75 80 Asp Gln Asn Val Ile Glu Val Val Leu Gly Asp Gly Val Phe Pro Ile 85 90 95 Arg Ser Lys Ser Arg Ile His Lys Thr Leu Ser Ala Gly Leu Gly Lys 100 105 110 Asn Arg Val Asp Leu Glu Leu Glu Glu His Val Phe Ala Glu Ser Glu 115 120 125 Gly Met Met Cys Leu Asp Arg His Gly Gly Glu Thr Asp Phe Pro Tyr 130 135 140 Lys Thr Gly Pro Gly Ala Val Glu Pro Tyr Pro Arg Arg Ile Leu Asp 145 150 155 160 Ala Ser Glu Asn Val Arg Ser Pro Glu Val Glu Thr Glu Glu Ala Leu 165 170 175 Ser Lys Leu Lys Glu Lys Leu Arg Gly Pro Pro Pro Asp Gly Met Arg 180 185 190 Asp Leu Arg Glu Glu Phe Ala Ala Lys Ala Val Glu Val Val Tyr Val 195 200 205 Pro Val Tyr Glu Ser Arg Leu Val Gly Pro Lys Lys Lys Val Arg Met 210 215 220 Met Arg Ile Asp Ala Ala Arg Lys Lys Ile Leu 225 230 235 <210> SEQ ID NO 51 <211> LENGTH: 378 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(378) <400> SEQUENCE: 51 atg agg tcg gag ggc agg ccc gga tac atc gaa aag ttc cta aag agg 48 Met Arg Ser Glu Gly Arg Pro Gly Tyr Ile Glu Lys Phe Leu Lys Arg 1 5 10 15 gcg gac aag gcg ata gac aat gca gtc gag cag ggc gtc aag agg gca 96 Ala Asp Lys Ala Ile Asp Asn Ala Val Glu Gln Gly Val Lys Arg Ala 20 25 30 gac gag ata cta gat gac gca gtc gag ctc ggc aag atc acc gtg ggc 144 Asp Glu Ile Leu Asp Asp Ala Val Glu Leu Gly Lys Ile Thr Val Gly 35 40 45 gag gcg caa aaa aga agc gat gtg ctg ctc aag cag gcc gag cgg gag 192 Glu Ala Gln Lys Arg Ser Asp Val Leu Leu Lys Gln Ala Glu Arg Glu 50 55 60 agc aag cgg ctc aag tca agg ggc gcc aaa aag ctc gaa aag ggc ata 240 Ser Lys Arg Leu Lys Ser Arg Gly Ala Lys Lys Leu Glu Lys Gly Ile 65 70 75 80 ggg gcg gca aaa aag atg gca gcc ggc aag ggc gac gcg cta gag acc 288 Gly Ala Ala Lys Lys Met Ala Ala Gly Lys Gly Asp Ala Leu Glu Thr 85 90 95 ctg gca aag ctc ggc gag ctg aga aag gcg ggg atc ata acg gag aag 336 Leu Ala Lys Leu Gly Glu Leu Arg Lys Ala Gly Ile Ile Thr Glu Lys 100 105 110 gag ttt cgc gcc aag aaa aag aag ctt ctc gcg gag atc tga 378 Glu Phe Arg Ala Lys Lys Lys Lys Leu Leu Ala Glu Ile 115 120 125 <210> SEQ ID NO 52 <211> LENGTH: 125 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 52 Met Arg Ser Glu Gly Arg Pro Gly Tyr Ile Glu Lys Phe Leu Lys Arg 1 5 10 15 Ala Asp Lys Ala Ile Asp Asn Ala Val Glu Gln Gly Val Lys Arg Ala 20 25 30 Asp Glu Ile Leu Asp Asp Ala Val Glu Leu Gly Lys Ile Thr Val Gly 35 40 45 Glu Ala Gln Lys Arg Ser Asp Val Leu Leu Lys Gln Ala Glu Arg Glu 50 55 60 Ser Lys Arg Leu Lys Ser Arg Gly Ala Lys Lys Leu Glu Lys Gly Ile 65 70 75 80 Gly Ala Ala Lys Lys Met Ala Ala Gly Lys Gly Asp Ala Leu Glu Thr 85 90 95 Leu Ala Lys Leu Gly Glu Leu Arg Lys Ala Gly Ile Ile Thr Glu Lys 100 105 110 Glu Phe Arg Ala Lys Lys Lys Lys Leu Leu Ala Glu Ile 115 120 125 <210> SEQ ID NO 53 <211> LENGTH: 606 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(606) <400> SEQUENCE: 53 atg tcc aag acg gag gcc tcc ccg ggg gga tat gcc tgc acg cca tac 48 Met Ser Lys Thr Glu Ala Ser Pro Gly Gly Tyr Ala Cys Thr Pro Tyr 1 5 10 15 acg cac gac cat gcc tcg ata gag ctc aag gag gaa tgg tcc tcg tcg 96 Thr His Asp His Ala Ser Ile Glu Leu Lys Glu Glu Trp Ser Ser Ser 20 25 30 agg aac gta ggc gag atg tac ttt gtg acc gcc act ttc tcg tcc aaa 144 Arg Asn Val Gly Glu Met Tyr Phe Val Thr Ala Thr Phe Ser Ser Lys 35 40 45 agc aag ccg tac ttt gag cag cag gcc agc cac tac ctg ctg gca agg 192 Ser Lys Pro Tyr Phe Glu Gln Gln Ala Ser His Tyr Leu Leu Ala Arg 50 55 60 ttc aaa aac ggc ccc aaa atg ata aag gcg gtg gag ggc cgc ggg ggc 240 Phe Lys Asn Gly Pro Lys Met Ile Lys Ala Val Glu Gly Arg Gly Gly 65 70 75 80 ggc cct tcc tat tta ttc agc atg gac gag gag ata ttc gaa agg gaa 288 Gly Pro Ser Tyr Leu Phe Ser Met Asp Glu Glu Ile Phe Glu Arg Glu 85 90 95 tcc ccc ggg atg agc tat gta tcc atg tac tat ctg gaa tac gga gat 336 Ser Pro Gly Met Ser Tyr Val Ser Met Tyr Tyr Leu Glu Tyr Gly Asp 100 105 110 tcc gag gag gac ata cgc gag gtg gcg tcg gta gtg gca aga aag gag 384 Ser Glu Glu Asp Ile Arg Glu Val Ala Ser Val Val Ala Arg Lys Glu 115 120 125 aag ata ggc agg gcg gga ata ggg cgc atg gat gta tgc tcg agg att 432 Lys Ile Gly Arg Ala Gly Ile Gly Arg Met Asp Val Cys Ser Arg Ile 130 135 140 ccg cca aag ttt gcc ttc ccg tac agc ggg aac att gtg gtg ctc gag 480 Pro Pro Lys Phe Ala Phe Pro Tyr Ser Gly Asn Ile Val Val Leu Glu 145 150 155 160 gta tcc agc gaa aag agc cac cag agc gtc aac aag tac tgc gaa aag 528 Val Ser Ser Glu Lys Ser His Gln Ser Val Asn Lys Tyr Cys Glu Lys 165 170 175 act aga agg gaa gtg atc cgc aag ggg ata acg atg acc aac ctt gta 576 Thr Arg Arg Glu Val Ile Arg Lys Gly Ile Thr Met Thr Asn Leu Val 180 185 190 agc ctg tcg ata ctg gag agg ctc aaa taa 606 Ser Leu Ser Ile Leu Glu Arg Leu Lys 195 200 <210> SEQ ID NO 54 <211> LENGTH: 201 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 54 Met Ser Lys Thr Glu Ala Ser Pro Gly Gly Tyr Ala Cys Thr Pro Tyr 1 5 10 15 Thr His Asp His Ala Ser Ile Glu Leu Lys Glu Glu Trp Ser Ser Ser 20 25 30 Arg Asn Val Gly Glu Met Tyr Phe Val Thr Ala Thr Phe Ser Ser Lys 35 40 45 Ser Lys Pro Tyr Phe Glu Gln Gln Ala Ser His Tyr Leu Leu Ala Arg 50 55 60 Phe Lys Asn Gly Pro Lys Met Ile Lys Ala Val Glu Gly Arg Gly Gly 65 70 75 80 Gly Pro Ser Tyr Leu Phe Ser Met Asp Glu Glu Ile Phe Glu Arg Glu 85 90 95 Ser Pro Gly Met Ser Tyr Val Ser Met Tyr Tyr Leu Glu Tyr Gly Asp 100 105 110 Ser Glu Glu Asp Ile Arg Glu Val Ala Ser Val Val Ala Arg Lys Glu 115 120 125 Lys Ile Gly Arg Ala Gly Ile Gly Arg Met Asp Val Cys Ser Arg Ile 130 135 140 Pro Pro Lys Phe Ala Phe Pro Tyr Ser Gly Asn Ile Val Val Leu Glu 145 150 155 160 Val Ser Ser Glu Lys Ser His Gln Ser Val Asn Lys Tyr Cys Glu Lys 165 170 175 Thr Arg Arg Glu Val Ile Arg Lys Gly Ile Thr Met Thr Asn Leu Val 180 185 190 Ser Leu Ser Ile Leu Glu Arg Leu Lys 195 200 <210> SEQ ID NO 55 <211> LENGTH: 822 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(822) <400> SEQUENCE: 55 ttg aaa agt acg ttg gtt cgg cgc tac aag ccc aag ata aag cag acc 48 Met Lys Ser Thr Leu Val Arg Arg Tyr Lys Pro Lys Ile Lys Gln Thr 1 5 10 15 ctc cgc gag gtg ccc ctc aaa aat gtg cat gtg tgg aag gag gcg cag 96 Leu Arg Glu Val Pro Leu Lys Asn Val His Val Trp Lys Glu Ala Gln 20 25 30 gca agg agg ctg gac agg tcc cgg gtg cgg gat atc gca aag tcg atc 144 Ala Arg Arg Leu Asp Arg Ser Arg Val Arg Asp Ile Ala Lys Ser Ile 35 40 45 aga tca gag ggg ctg cag aac ccg ccc gtc ata cag agg ggc ggc agg 192 Arg Ser Glu Gly Leu Gln Asn Pro Pro Val Ile Gln Arg Gly Gly Arg 50 55 60 ggg ctg tac ctc ctc ata tcg ggg cac cac cgg ctt gcg gcc ctc aag 240 Gly Leu Tyr Leu Leu Ile Ser Gly His His Arg Leu Ala Ala Leu Lys 65 70 75 80 tac ctg ggc gca aaa aag tcc aag ttt ctg gtg ata acc aag gat aca 288 Tyr Leu Gly Ala Lys Lys Ser Lys Phe Leu Val Ile Thr Lys Asp Thr 85 90 95 gag tac ggc ctg gat gat gca aag gcc gca tcg gtt gta gag aac ctg 336 Glu Tyr Gly Leu Asp Asp Ala Lys Ala Ala Ser Val Val Glu Asn Leu 100 105 110 cac cgt ctc cag atg agc ccg cgg gag ctt gca gac gca tgc aag ttc 384 His Arg Leu Gln Met Ser Pro Arg Glu Leu Ala Asp Ala Cys Lys Phe 115 120 125 ctg gcc gag cag acg aca aaa tcc gag gcc gca aaa aag ctc ggc atg 432 Leu Ala Glu Gln Thr Thr Lys Ser Glu Ala Ala Lys Lys Leu Gly Met 130 135 140 tcg atg ccc acg ttc aag aaa tac cac ggc ttt gcg ggc gta ccg gac 480 Ser Met Pro Thr Phe Lys Lys Tyr His Gly Phe Ala Gly Val Pro Asp 145 150 155 160 aag atc aag gcg atg gta ccg ggc acc ata tcc cgg gac gag gcg aca 528 Lys Ile Lys Ala Met Val Pro Gly Thr Ile Ser Arg Asp Glu Ala Thr 165 170 175 agg ctc tac cag gcg gtg ccg acc ata tcc cag gcg ctc aag gtg gta 576 Arg Leu Tyr Gln Ala Val Pro Thr Ile Ser Gln Ala Leu Lys Val Val 180 185 190 tca aag ata gca aag ctc gac agg ccg tcg agg cgg atc tac ctg agg 624 Ser Lys Ile Ala Lys Leu Asp Arg Pro Ser Arg Arg Ile Tyr Leu Arg 195 200 205 ttg ctt gcc cag agc ccc cgc tcc ggc cac aag ata ata cta aag agg 672 Leu Leu Ala Gln Ser Pro Arg Ser Gly His Lys Ile Ile Leu Lys Arg 210 215 220 atg cgc aag gtg ggc atc aag aaa aag ata cca ata gag ctg ggc aag 720 Met Arg Lys Val Gly Ile Lys Lys Lys Ile Pro Ile Glu Leu Gly Lys 225 230 235 240 aac ggc gca aga aag ctc tcc agg ctg gcc gag cgc gag ggg aca gac 768 Asn Gly Ala Arg Lys Leu Ser Arg Leu Ala Glu Arg Glu Gly Thr Asp 245 250 255 gag acc cgg ctt gcc aac agg ata gtc cgg gaa tac ctg agg aag cgg 816 Glu Thr Arg Leu Ala Asn Arg Ile Val Arg Glu Tyr Leu Arg Lys Arg 260 265 270 cga tga 822 Arg <210> SEQ ID NO 56 <211> LENGTH: 273 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 56 Met Lys Ser Thr Leu Val Arg Arg Tyr Lys Pro Lys Ile Lys Gln Thr 1 5 10 15 Leu Arg Glu Val Pro Leu Lys Asn Val His Val Trp Lys Glu Ala Gln 20 25 30 Ala Arg Arg Leu Asp Arg Ser Arg Val Arg Asp Ile Ala Lys Ser Ile 35 40 45 Arg Ser Glu Gly Leu Gln Asn Pro Pro Val Ile Gln Arg Gly Gly Arg 50 55 60 Gly Leu Tyr Leu Leu Ile Ser Gly His His Arg Leu Ala Ala Leu Lys 65 70 75 80 Tyr Leu Gly Ala Lys Lys Ser Lys Phe Leu Val Ile Thr Lys Asp Thr 85 90 95 Glu Tyr Gly Leu Asp Asp Ala Lys Ala Ala Ser Val Val Glu Asn Leu 100 105 110 His Arg Leu Gln Met Ser Pro Arg Glu Leu Ala Asp Ala Cys Lys Phe 115 120 125 Leu Ala Glu Gln Thr Thr Lys Ser Glu Ala Ala Lys Lys Leu Gly Met 130 135 140 Ser Met Pro Thr Phe Lys Lys Tyr His Gly Phe Ala Gly Val Pro Asp 145 150 155 160 Lys Ile Lys Ala Met Val Pro Gly Thr Ile Ser Arg Asp Glu Ala Thr 165 170 175 Arg Leu Tyr Gln Ala Val Pro Thr Ile Ser Gln Ala Leu Lys Val Val 180 185 190 Ser Lys Ile Ala Lys Leu Asp Arg Pro Ser Arg Arg Ile Tyr Leu Arg 195 200 205 Leu Leu Ala Gln Ser Pro Arg Ser Gly His Lys Ile Ile Leu Lys Arg 210 215 220 Met Arg Lys Val Gly Ile Lys Lys Lys Ile Pro Ile Glu Leu Gly Lys 225 230 235 240 Asn Gly Ala Arg Lys Leu Ser Arg Leu Ala Glu Arg Glu Gly Thr Asp 245 250 255 Glu Thr Arg Leu Ala Asn Arg Ile Val Arg Glu Tyr Leu Arg Lys Arg 260 265 270 Arg <210> SEQ ID NO 57 <211> LENGTH: 669 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(669) <400> SEQUENCE: 57 gtg gcg cga tcg ccc gtg ctg ata ata aac tgc aaa aac tac aag gag 48 Met Ala Arg Ser Pro Val Leu Ile Ile Asn Cys Lys Asn Tyr Lys Glu 1 5 10 15 gcg gcc ggc ggc aga att gac agc cta gcg gcg gca gcc gcc ggg gcg 96 Ala Ala Gly Gly Arg Ile Asp Ser Leu Ala Ala Ala Ala Ala Gly Ala 20 25 30 gcc gca aaa tac ggc gtc agg ata gct ctt gcc ccg ccg cag cac ctg 144 Ala Ala Lys Tyr Gly Val Arg Ile Ala Leu Ala Pro Pro Gln His Leu 35 40 45 ctg ggc gca gta aag ggg gaa gat ctt aca gtt ctg gcg cag cat ata 192 Leu Gly Ala Val Lys Gly Glu Asp Leu Thr Val Leu Ala Gln His Ile 50 55 60 gac gac aag ggg gtt gga agc acc aca gga tat gtc gtg ccg gag ctg 240 Asp Asp Lys Gly Val Gly Ser Thr Thr Gly Tyr Val Val Pro Glu Leu 65 70 75 80 ctg gga gaa tcc ggc gtc tct ggc gcg ctc atc aac cac agc gag cac 288 Leu Gly Glu Ser Gly Val Ser Gly Ala Leu Ile Asn His Ser Glu His 85 90 95 cgc gta tca gct gac cag gtg gca agc ctt gtg ccc agg ctc agg ggt 336 Arg Val Ser Ala Asp Gln Val Ala Ser Leu Val Pro Arg Leu Arg Gly 100 105 110 ctg gat atg atc tcc gtg gtc tgt gta aag gat tcc gcc gag gcg gca 384 Leu Asp Met Ile Ser Val Val Cys Val Lys Asp Ser Ala Glu Ala Ala 115 120 125 aat ctc tcc cgg cac cgg ccc gac tac ata gct atc gag cct ccc gag 432 Asn Leu Ser Arg His Arg Pro Asp Tyr Ile Ala Ile Glu Pro Pro Glu 130 135 140 ctg ata ggc tcg ggc agg tcc gtc tca tcg gag agg ccc gag ctg ata 480 Leu Ile Gly Ser Gly Arg Ser Val Ser Ser Glu Arg Pro Glu Leu Ile 145 150 155 160 ggg gag gca gca gag gcc atc agg ggg gcg gat gga aca aag ctg ctc 528 Gly Glu Ala Ala Glu Ala Ile Arg Gly Ala Asp Gly Thr Lys Leu Leu 165 170 175 tgc ggg gcg ggc ata aca tca ggc gct gat gtg cgc aag gcc ctc gag 576 Cys Gly Ala Gly Ile Thr Ser Gly Ala Asp Val Arg Lys Ala Leu Glu 180 185 190 ctc ggc tcc aag ggg atc ctc gtg gca agc ggg gtg gta aaa tca tca 624 Leu Gly Ser Lys Gly Ile Leu Val Ala Ser Gly Val Val Lys Ser Ser 195 200 205 gac ccc gct gcg gcc ata gcc gag ctg gca cag gcc atg tcc tga 669 Asp Pro Ala Ala Ala Ile Ala Glu Leu Ala Gln Ala Met Ser 210 215 220 <210> SEQ ID NO 58 <211> LENGTH: 222 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 58 Met Ala Arg Ser Pro Val Leu Ile Ile Asn Cys Lys Asn Tyr Lys Glu 1 5 10 15 Ala Ala Gly Gly Arg Ile Asp Ser Leu Ala Ala Ala Ala Ala Gly Ala 20 25 30 Ala Ala Lys Tyr Gly Val Arg Ile Ala Leu Ala Pro Pro Gln His Leu 35 40 45 Leu Gly Ala Val Lys Gly Glu Asp Leu Thr Val Leu Ala Gln His Ile 50 55 60 Asp Asp Lys Gly Val Gly Ser Thr Thr Gly Tyr Val Val Pro Glu Leu 65 70 75 80 Leu Gly Glu Ser Gly Val Ser Gly Ala Leu Ile Asn His Ser Glu His 85 90 95 Arg Val Ser Ala Asp Gln Val Ala Ser Leu Val Pro Arg Leu Arg Gly 100 105 110 Leu Asp Met Ile Ser Val Val Cys Val Lys Asp Ser Ala Glu Ala Ala 115 120 125 Asn Leu Ser Arg His Arg Pro Asp Tyr Ile Ala Ile Glu Pro Pro Glu 130 135 140 Leu Ile Gly Ser Gly Arg Ser Val Ser Ser Glu Arg Pro Glu Leu Ile 145 150 155 160 Gly Glu Ala Ala Glu Ala Ile Arg Gly Ala Asp Gly Thr Lys Leu Leu 165 170 175 Cys Gly Ala Gly Ile Thr Ser Gly Ala Asp Val Arg Lys Ala Leu Glu 180 185 190 Leu Gly Ser Lys Gly Ile Leu Val Ala Ser Gly Val Val Lys Ser Ser 195 200 205 Asp Pro Ala Ala Ala Ile Ala Glu Leu Ala Gln Ala Met Ser 210 215 220 <210> SEQ ID NO 59 <211> LENGTH: 549 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(548) <400> SEQUENCE: 59 atg ctg gat ccc cgg acg cgg ccc cgg gtc gtc aat gtc gtc agc aca 48 Met Leu Asp Pro Arg Thr Arg Pro Arg Val Val Asn Val Val Ser Thr 1 5 10 15 tca gac ctt gta caa agg gtg agc gca aaa aag atg gcc gcc atg ccg 96 Ser Asp Leu Val Gln Arg Val Ser Ala Lys Lys Met Ala Ala Met Pro 20 25 30 tgc tgc atg tat gat gag gcc gta tac ggc ggc agg tgc ggc tac ata 144 Cys Cys Met Tyr Asp Glu Ala Val Tyr Gly Gly Arg Cys Gly Tyr Ile 35 40 45 aag acg ccc ggc atg cag ggg agg gtg act gta ttc att tct ggc aag 192 Lys Thr Pro Gly Met Gln Gly Arg Val Thr Val Phe Ile Ser Gly Lys 50 55 60 atg ata tcc gtc ggc gcc aga tcc gtg agg gcc tcg ttt ggg cag ctg 240 Met Ile Ser Val Gly Ala Arg Ser Val Arg Ala Ser Phe Gly Gln Leu 65 70 75 80 cac gag gcg cgg ctc cac ctg gtg cgc aac ggg gct gcc ggc gac tgc 288 His Glu Ala Arg Leu His Leu Val Arg Asn Gly Ala Ala Gly Asp Cys 85 90 95 aag ata agg ccc gtc gtg cgc aat att gta gcc acg gtg gat gcc ggt 336 Lys Ile Arg Pro Val Val Arg Asn Ile Val Ala Thr Val Asp Ala Gly 100 105 110 agg aat gtt ccc ata gac agg ata tcg tcg cgc atg cct ggc gct gta 384 Arg Asn Val Pro Ile Asp Arg Ile Ser Ser Arg Met Pro Gly Ala Val 115 120 125 tat gat ccc ggg tcg ttt ccc ggg atg ata ctc aag ggg ctg gac agc 432 Tyr Asp Pro Gly Ser Phe Pro Gly Met Ile Leu Lys Gly Leu Asp Ser 130 135 140 tgc agc ttt cta gtc ttt gcg tcg gga aag atg gtg ata gcg ggc gcc 480 Cys Ser Phe Leu Val Phe Ala Ser Gly Lys Met Val Ile Ala Gly Ala 145 150 155 160 aag tcg ccg gat gag ctg cgc agg tcg tcg ttt gac ctg ctg acg cgc 528 Lys Ser Pro Asp Glu Leu Arg Arg Ser Ser Phe Asp Leu Leu Thr Arg 165 170 175 ctc aat aac gcg ggg gcc ta g 549 Leu Asn Asn Ala Gly Ala 180 <210> SEQ ID NO 60 <211> LENGTH: 182 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 60 Met Leu Asp Pro Arg Thr Arg Pro Arg Val Val Asn Val Val Ser Thr 1 5 10 15 Ser Asp Leu Val Gln Arg Val Ser Ala Lys Lys Met Ala Ala Met Pro 20 25 30 Cys Cys Met Tyr Asp Glu Ala Val Tyr Gly Gly Arg Cys Gly Tyr Ile 35 40 45 Lys Thr Pro Gly Met Gln Gly Arg Val Thr Val Phe Ile Ser Gly Lys 50 55 60 Met Ile Ser Val Gly Ala Arg Ser Val Arg Ala Ser Phe Gly Gln Leu 65 70 75 80 His Glu Ala Arg Leu His Leu Val Arg Asn Gly Ala Ala Gly Asp Cys 85 90 95 Lys Ile Arg Pro Val Val Arg Asn Ile Val Ala Thr Val Asp Ala Gly 100 105 110 Arg Asn Val Pro Ile Asp Arg Ile Ser Ser Arg Met Pro Gly Ala Val 115 120 125 Tyr Asp Pro Gly Ser Phe Pro Gly Met Ile Leu Lys Gly Leu Asp Ser 130 135 140 Cys Ser Phe Leu Val Phe Ala Ser Gly Lys Met Val Ile Ala Gly Ala 145 150 155 160 Lys Ser Pro Asp Glu Leu Arg Arg Ser Ser Phe Asp Leu Leu Thr Arg 165 170 175 Leu Asn Asn Ala Gly Ala 180 <210> SEQ ID NO 61 <211> LENGTH: 2538 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(2538) <400> SEQUENCE: 61 ctg act gca cag gat gaa gag att ccc ccg tca ctg ctt gta tct gca 48 Met Thr Ala Gln Asp Glu Glu Ile Pro Pro Ser Leu Leu Val Ser Ala 1 5 10 15 acc tat gat ggc cag gca agg gcc gtg gtc ctc aag ttc tac gag tcg 96 Thr Tyr Asp Gly Gln Ala Arg Ala Val Val Leu Lys Phe Tyr Glu Ser 20 25 30 gaa tcg caa aag atc atc cac tgg acg gac aac acg ggg cac aag ccc 144 Glu Ser Gln Lys Ile Ile His Trp Thr Asp Asn Thr Gly His Lys Pro 35 40 45 tac tgt tat acg agg ctg ccg ccc tcc gag ctc ggc ttt ctt ggg ggc 192 Tyr Cys Tyr Thr Arg Leu Pro Pro Ser Glu Leu Gly Phe Leu Gly Gly 50 55 60 agg gag gac gtg ctc ggg ata gag cag gtc atg cgg cac gac ctg ata 240 Arg Glu Asp Val Leu Gly Ile Glu Gln Val Met Arg His Asp Leu Ile 65 70 75 80 gcc gac aag gag gtg ccc gtc tcc aag ata acc gtc tct gat cct ctt 288 Ala Asp Lys Glu Val Pro Val Ser Lys Ile Thr Val Ser Asp Pro Leu 85 90 95 gcg ata ggc ggg acc cac tcg gag aag agc atc aga aac gtg ata gac 336 Ala Ile Gly Gly Thr His Ser Glu Lys Ser Ile Arg Asn Val Ile Asp 100 105 110 acg tgg gaa tcc gac ata aag tat tac gag aac tat ctg tat gac gcg 384 Thr Trp Glu Ser Asp Ile Lys Tyr Tyr Glu Asn Tyr Leu Tyr Asp Ala 115 120 125 ggc ctg gta gtg ggc agg tac tat tcg gta tca ggc ggg gag gtg att 432 Gly Leu Val Val Gly Arg Tyr Tyr Ser Val Ser Gly Gly Glu Val Ile 130 135 140 ccg cat gac atg cca ata tcc gac gag gta aaa ctg gcc ctc aag agc 480 Pro His Asp Met Pro Ile Ser Asp Glu Val Lys Leu Ala Leu Lys Ser 145 150 155 160 ctt ctc tgg gac aag ctc ata gac gag ggc atg gcc gac agg aaa gag 528 Leu Leu Trp Asp Lys Leu Ile Asp Glu Gly Met Ala Asp Arg Lys Glu 165 170 175 ttc cgc gag ttc ata gcg ggg tgg gcg gac ctg ctc aac cag ccc ata 576 Phe Arg Glu Phe Ile Ala Gly Trp Ala Asp Leu Leu Asn Gln Pro Ile 180 185 190 ccc cgg ata agg cgc ctc agc ttt gac atc gag gtg gat tca gag gag 624 Pro Arg Ile Arg Arg Leu Ser Phe Asp Ile Glu Val Asp Ser Glu Glu 195 200 205 ggc agg atc ccc gat gcc aag atc tcg gac agg agg gtc aca gca gtg 672 Gly Arg Ile Pro Asp Ala Lys Ile Ser Asp Arg Arg Val Thr Ala Val 210 215 220 ggg ttt gcc gcc acc gac ggc ctc aga aag gtc ctt gtc ctg aag agc 720 Gly Phe Ala Ala Thr Asp Gly Leu Arg Lys Val Leu Val Leu Lys Ser 225 230 235 240 ggc gcg gac gag ggc gca aac gat gtg acc ccc ggg gtc gag gtg gtg 768 Gly Ala Asp Glu Gly Ala Asn Asp Val Thr Pro Gly Val Glu Val Val 245 250 255 ttc tac gac gag gac aag gag gcg gac atg atc cgc gac gcg cta gca 816 Phe Tyr Asp Glu Asp Lys Glu Ala Asp Met Ile Arg Asp Ala Leu Ala 260 265 270 ata ata ggc tcg tac ccg ttt gtg ctt aca tac aac ggg gac gac ttt 864 Ile Ile Gly Ser Tyr Pro Phe Val Leu Thr Tyr Asn Gly Asp Asp Phe 275 280 285 gac atg ccg tac atg tac aat cgg gcc cgg cgc ctc ggc gtg gcg gat 912 Asp Met Pro Tyr Met Tyr Asn Arg Ala Arg Arg Leu Gly Val Ala Asp 290 295 300 tcc gac ata ccc ctg tac atg atg cgg gat tcg gcc acg ctc cgg cac 960 Ser Asp Ile Pro Leu Tyr Met Met Arg Asp Ser Ala Thr Leu Arg His 305 310 315 320 ggc gtc cat ctg gac ctg tac agg acc ttc tcg aac agg tcg ttc cag 1008 Gly Val His Leu Asp Leu Tyr Arg Thr Phe Ser Asn Arg Ser Phe Gln 325 330 335 ctg tat gca ttt gcg gca aag tat aca gat tac tcc ctg aac agc gtg 1056 Leu Tyr Ala Phe Ala Ala Lys Tyr Thr Asp Tyr Ser Leu Asn Ser Val 340 345 350 tcc aag gcg atg ctc ggc gag ggc aag gtc gat tat ggc gtg tct ctc 1104 Ser Lys Ala Met Leu Gly Glu Gly Lys Val Asp Tyr Gly Val Ser Leu 355 360 365 ggg gat ctc act cta tac cag act gca aac tat tgc tat cat gac gcg 1152 Gly Asp Leu Thr Leu Tyr Gln Thr Ala Asn Tyr Cys Tyr His Asp Ala 370 375 380 cgc ctg acg ctg gag ctt agc acc ttt ggg aac gag ata ctg atg gac 1200 Arg Leu Thr Leu Glu Leu Ser Thr Phe Gly Asn Glu Ile Leu Met Asp 385 390 395 400 ctc ctg gtg gtg acc agc agg att gcc cgg atg ccc atc gat gat atg 1248 Leu Leu Val Val Thr Ser Arg Ile Ala Arg Met Pro Ile Asp Asp Met 405 410 415 tcc cgc atg ggc gtc tcg cag tgg ata agg agc ctg ctg tac tat gag 1296 Ser Arg Met Gly Val Ser Gln Trp Ile Arg Ser Leu Leu Tyr Tyr Glu 420 425 430 cac agg cag cgc aac gcg ctg ata ccc cgc agg gac gag ctg gaa aag 1344 His Arg Gln Arg Asn Ala Leu Ile Pro Arg Arg Asp Glu Leu Glu Lys 435 440 445 agg tct caa cag gta agc aac gac gcc gta atc aag gac aaa aag ttc 1392 Arg Ser Gln Gln Val Ser Asn Asp Ala Val Ile Lys Asp Lys Lys Phe 450 455 460 cgc ggt ggt ctc gta gtc gag cct gaa gag ggc ata cac ttt gat gtt 1440 Arg Gly Gly Leu Val Val Glu Pro Glu Glu Gly Ile His Phe Asp Val 465 470 475 480 aca gtt atg gat ttt gca agc ctg tat cct agc ata ata aag gtg cga 1488 Thr Val Met Asp Phe Ala Ser Leu Tyr Pro Ser Ile Ile Lys Val Arg 485 490 495 aac ctc tcg tac gag acc gtc agg tgc gtt cat ccc gaa tgc aga aag 1536 Asn Leu Ser Tyr Glu Thr Val Arg Cys Val His Pro Glu Cys Arg Lys 500 505 510 aac acc atc ccc gat acc aac cac tgg gta tgc acg aaa aac aac ggg 1584 Asn Thr Ile Pro Asp Thr Asn His Trp Val Cys Thr Lys Asn Asn Gly 515 520 525 ctt aca tcg atg ata ata gga tcg ctc cgc gac ctg cgc gtc aac tat 1632 Leu Thr Ser Met Ile Ile Gly Ser Leu Arg Asp Leu Arg Val Asn Tyr 530 535 540 tac aag agc ctc tca aag agc cag tct ata acg gag gag cag cgg cag 1680 Tyr Lys Ser Leu Ser Lys Ser Gln Ser Ile Thr Glu Glu Gln Arg Gln 545 550 555 560 cag tat act gtg atc agc cag gcc ctc aag gtg gtg cta aac gca agc 1728 Gln Tyr Thr Val Ile Ser Gln Ala Leu Lys Val Val Leu Asn Ala Ser 565 570 575 tac ggg gtg atg ggc gcc gag ata ttc ccg ctg tac ttt ctg cct gcc 1776 Tyr Gly Val Met Gly Ala Glu Ile Phe Pro Leu Tyr Phe Leu Pro Ala 580 585 590 gcc gag gcc acc acg gcg gtc ggg cgc tat atc atc atg cag acc ata 1824 Ala Glu Ala Thr Thr Ala Val Gly Arg Tyr Ile Ile Met Gln Thr Ile 595 600 605 tcg cac tgc gag cag atg ggc gta aag gtg ctg tac ggg gac acc gat 1872 Ser His Cys Glu Gln Met Gly Val Lys Val Leu Tyr Gly Asp Thr Asp 610 615 620 tcg ctg ttc ata aag aat cca gag gag cgg cag atc cat gat ata gtc 1920 Ser Leu Phe Ile Lys Asn Pro Glu Glu Arg Gln Ile His Asp Ile Val 625 630 635 640 gag cac gcc aaa aag gag cac ggc gtc gag ctc gag gtg gac aaa gag 1968 Glu His Ala Lys Lys Glu His Gly Val Glu Leu Glu Val Asp Lys Glu 645 650 655 tac agg tat gtc gtg cta tct aac agg aag aaa aac tat ttc ggg gtg 2016 Tyr Arg Tyr Val Val Leu Ser Asn Arg Lys Lys Asn Tyr Phe Gly Val 660 665 670 aca aag tcc ggc aag gtc gac gtc aag ggc ctg acg ggg aaa aag tcg 2064 Thr Lys Ser Gly Lys Val Asp Val Lys Gly Leu Thr Gly Lys Lys Ser 675 680 685 cac acg ccc ccg ttc ata aag gag ctg ttc tat tcg ctg ctc gac ata 2112 His Thr Pro Pro Phe Ile Lys Glu Leu Phe Tyr Ser Leu Leu Asp Ile 690 695 700 ctg tcg gct gta cag acc gag gac gag ttt gaa tcg gca aag cta aag 2160 Leu Ser Ala Val Gln Thr Glu Asp Glu Phe Glu Ser Ala Lys Leu Lys 705 710 715 720 atc tca aag gcc ata gcg gca tcc ggg aag agg ctg gag gag agg ggg 2208 Ile Ser Lys Ala Ile Ala Ala Ser Gly Lys Arg Leu Glu Glu Arg Gly 725 730 735 gtc ccg ctg gcg gat ctg gcg ttc aat gtg atg ata agc aag gcg ccc 2256 Val Pro Leu Ala Asp Leu Ala Phe Asn Val Met Ile Ser Lys Ala Pro 740 745 750 tct gaa tac gta aag acc gtc ccg cag cac ata cgg gcg gcc aga ctg 2304 Ser Glu Tyr Val Lys Thr Val Pro Gln His Ile Arg Ala Ala Arg Leu 755 760 765 ctc gag aac gca agg gag gtc aaa aaa ggc gac ata ata tcg tac gta 2352 Leu Glu Asn Ala Arg Glu Val Lys Lys Gly Asp Ile Ile Ser Tyr Val 770 775 780 aag gtg atg aac aag aca ggc gtc aag cct gtc gag atg gcc cag gca 2400 Lys Val Met Asn Lys Thr Gly Val Lys Pro Val Glu Met Ala Gln Ala 785 790 795 800 gga gag gtg gac acg tca aag tat cta gag ttc atg gag tct act ctg 2448 Gly Glu Val Asp Thr Ser Lys Tyr Leu Glu Phe Met Glu Ser Thr Leu 805 810 815 gac cag ctc acc tcg tcc atg ggc ctt gac ttt gac gag atg ctg ggc 2496 Asp Gln Leu Thr Ser Ser Met Gly Leu Asp Phe Asp Glu Met Leu Gly 820 825 830 aag cca aag cag act gga atg gag cag ttc ttt ttc aaa tga 2538 Lys Pro Lys Gln Thr Gly Met Glu Gln Phe Phe Phe Lys 835 840 845 <210> SEQ ID NO 62 <211> LENGTH: 845 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 62 Met Thr Ala Gln Asp Glu Glu Ile Pro Pro Ser Leu Leu Val Ser Ala 1 5 10 15 Thr Tyr Asp Gly Gln Ala Arg Ala Val Val Leu Lys Phe Tyr Glu Ser 20 25 30 Glu Ser Gln Lys Ile Ile His Trp Thr Asp Asn Thr Gly His Lys Pro 35 40 45 Tyr Cys Tyr Thr Arg Leu Pro Pro Ser Glu Leu Gly Phe Leu Gly Gly 50 55 60 Arg Glu Asp Val Leu Gly Ile Glu Gln Val Met Arg His Asp Leu Ile 65 70 75 80 Ala Asp Lys Glu Val Pro Val Ser Lys Ile Thr Val Ser Asp Pro Leu 85 90 95 Ala Ile Gly Gly Thr His Ser Glu Lys Ser Ile Arg Asn Val Ile Asp 100 105 110 Thr Trp Glu Ser Asp Ile Lys Tyr Tyr Glu Asn Tyr Leu Tyr Asp Ala 115 120 125 Gly Leu Val Val Gly Arg Tyr Tyr Ser Val Ser Gly Gly Glu Val Ile 130 135 140 Pro His Asp Met Pro Ile Ser Asp Glu Val Lys Leu Ala Leu Lys Ser 145 150 155 160 Leu Leu Trp Asp Lys Leu Ile Asp Glu Gly Met Ala Asp Arg Lys Glu 165 170 175 Phe Arg Glu Phe Ile Ala Gly Trp Ala Asp Leu Leu Asn Gln Pro Ile 180 185 190 Pro Arg Ile Arg Arg Leu Ser Phe Asp Ile Glu Val Asp Ser Glu Glu 195 200 205 Gly Arg Ile Pro Asp Ala Lys Ile Ser Asp Arg Arg Val Thr Ala Val 210 215 220 Gly Phe Ala Ala Thr Asp Gly Leu Arg Lys Val Leu Val Leu Lys Ser 225 230 235 240 Gly Ala Asp Glu Gly Ala Asn Asp Val Thr Pro Gly Val Glu Val Val 245 250 255 Phe Tyr Asp Glu Asp Lys Glu Ala Asp Met Ile Arg Asp Ala Leu Ala 260 265 270 Ile Ile Gly Ser Tyr Pro Phe Val Leu Thr Tyr Asn Gly Asp Asp Phe 275 280 285 Asp Met Pro Tyr Met Tyr Asn Arg Ala Arg Arg Leu Gly Val Ala Asp 290 295 300 Ser Asp Ile Pro Leu Tyr Met Met Arg Asp Ser Ala Thr Leu Arg His 305 310 315 320 Gly Val His Leu Asp Leu Tyr Arg Thr Phe Ser Asn Arg Ser Phe Gln 325 330 335 Leu Tyr Ala Phe Ala Ala Lys Tyr Thr Asp Tyr Ser Leu Asn Ser Val 340 345 350 Ser Lys Ala Met Leu Gly Glu Gly Lys Val Asp Tyr Gly Val Ser Leu 355 360 365 Gly Asp Leu Thr Leu Tyr Gln Thr Ala Asn Tyr Cys Tyr His Asp Ala 370 375 380 Arg Leu Thr Leu Glu Leu Ser Thr Phe Gly Asn Glu Ile Leu Met Asp 385 390 395 400 Leu Leu Val Val Thr Ser Arg Ile Ala Arg Met Pro Ile Asp Asp Met 405 410 415 Ser Arg Met Gly Val Ser Gln Trp Ile Arg Ser Leu Leu Tyr Tyr Glu 420 425 430 His Arg Gln Arg Asn Ala Leu Ile Pro Arg Arg Asp Glu Leu Glu Lys 435 440 445 Arg Ser Gln Gln Val Ser Asn Asp Ala Val Ile Lys Asp Lys Lys Phe 450 455 460 Arg Gly Gly Leu Val Val Glu Pro Glu Glu Gly Ile His Phe Asp Val 465 470 475 480 Thr Val Met Asp Phe Ala Ser Leu Tyr Pro Ser Ile Ile Lys Val Arg 485 490 495 Asn Leu Ser Tyr Glu Thr Val Arg Cys Val His Pro Glu Cys Arg Lys 500 505 510 Asn Thr Ile Pro Asp Thr Asn His Trp Val Cys Thr Lys Asn Asn Gly 515 520 525 Leu Thr Ser Met Ile Ile Gly Ser Leu Arg Asp Leu Arg Val Asn Tyr 530 535 540 Tyr Lys Ser Leu Ser Lys Ser Gln Ser Ile Thr Glu Glu Gln Arg Gln 545 550 555 560 Gln Tyr Thr Val Ile Ser Gln Ala Leu Lys Val Val Leu Asn Ala Ser 565 570 575 Tyr Gly Val Met Gly Ala Glu Ile Phe Pro Leu Tyr Phe Leu Pro Ala 580 585 590 Ala Glu Ala Thr Thr Ala Val Gly Arg Tyr Ile Ile Met Gln Thr Ile 595 600 605 Ser His Cys Glu Gln Met Gly Val Lys Val Leu Tyr Gly Asp Thr Asp 610 615 620 Ser Leu Phe Ile Lys Asn Pro Glu Glu Arg Gln Ile His Asp Ile Val 625 630 635 640 Glu His Ala Lys Lys Glu His Gly Val Glu Leu Glu Val Asp Lys Glu 645 650 655 Tyr Arg Tyr Val Val Leu Ser Asn Arg Lys Lys Asn Tyr Phe Gly Val 660 665 670 Thr Lys Ser Gly Lys Val Asp Val Lys Gly Leu Thr Gly Lys Lys Ser 675 680 685 His Thr Pro Pro Phe Ile Lys Glu Leu Phe Tyr Ser Leu Leu Asp Ile 690 695 700 Leu Ser Ala Val Gln Thr Glu Asp Glu Phe Glu Ser Ala Lys Leu Lys 705 710 715 720 Ile Ser Lys Ala Ile Ala Ala Ser Gly Lys Arg Leu Glu Glu Arg Gly 725 730 735 Val Pro Leu Ala Asp Leu Ala Phe Asn Val Met Ile Ser Lys Ala Pro 740 745 750 Ser Glu Tyr Val Lys Thr Val Pro Gln His Ile Arg Ala Ala Arg Leu 755 760 765 Leu Glu Asn Ala Arg Glu Val Lys Lys Gly Asp Ile Ile Ser Tyr Val 770 775 780 Lys Val Met Asn Lys Thr Gly Val Lys Pro Val Glu Met Ala Gln Ala 785 790 795 800 Gly Glu Val Asp Thr Ser Lys Tyr Leu Glu Phe Met Glu Ser Thr Leu 805 810 815 Asp Gln Leu Thr Ser Ser Met Gly Leu Asp Phe Asp Glu Met Leu Gly 820 825 830 Lys Pro Lys Gln Thr Gly Met Glu Gln Phe Phe Phe Lys 835 840 845 <210> SEQ ID NO 63 <211> LENGTH: 642 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(642) <400> SEQUENCE: 63 ttg ccc gtt atg tgt gcg gtc tcc acg cgc ggc cct gac gcg gcc tgt 48 Met Pro Val Met Cys Ala Val Ser Thr Arg Gly Pro Asp Ala Ala Cys 1 5 10 15 tgt ttt atg gtt tcg tac acc ggg gca tat acc ata ata tgc cgg gcg 96 Cys Phe Met Val Ser Tyr Thr Gly Ala Tyr Thr Ile Ile Cys Arg Ala 20 25 30 gtg gca cca tgg ccg ttg agc ggt ttt gag cgc ccg tcc tgg gac gaa 144 Val Ala Pro Trp Pro Leu Ser Gly Phe Glu Arg Pro Ser Trp Asp Glu 35 40 45 tat ttc atg ctg cag gcg gag ctg gca aag ctc cga tcc aac tgc atg 192 Tyr Phe Met Leu Gln Ala Glu Leu Ala Lys Leu Arg Ser Asn Cys Met 50 55 60 gtc aga aag gtg ggg gcc gtc ata gtc agg gat cac agg cag ctg gcc 240 Val Arg Lys Val Gly Ala Val Ile Val Arg Asp His Arg Gln Leu Ala 65 70 75 80 aca gga tac aac ggg acg ccc ccc ggc gta aag aac tgc ttc gag ggc 288 Thr Gly Tyr Asn Gly Thr Pro Pro Gly Val Lys Asn Cys Phe Glu Gly 85 90 95 ggg tgc gaa agg tgc ata gag cgc atg gag ggc aag atc cgc tca ggc 336 Gly Cys Glu Arg Cys Ile Glu Arg Met Glu Gly Lys Ile Arg Ser Gly 100 105 110 gag ggc ctg gac cgg tgc ctg tgc aac cat gca gag gcc aac gcg ata 384 Glu Gly Leu Asp Arg Cys Leu Cys Asn His Ala Glu Ala Asn Ala Ile 115 120 125 atg cac tgt gcg ata ctg gga ata ggc gca ggg gga ggc aac gcc acc 432 Met His Cys Ala Ile Leu Gly Ile Gly Ala Gly Gly Gly Asn Ala Thr 130 135 140 atg tat acg acg ttc tct ccg tgt tta gag tgc aca aag atg gcg gtg 480 Met Tyr Thr Thr Phe Ser Pro Cys Leu Glu Cys Thr Lys Met Ala Val 145 150 155 160 acc ata gga atc agg cgg ttt gtc tgc ctg gat aca tat ccg gag aac 528 Thr Ile Gly Ile Arg Arg Phe Val Cys Leu Asp Thr Tyr Pro Glu Asn 165 170 175 gcc tcc aag ctg gta aaa gat gca tcg gcc agc ata acc atg atg gac 576 Ala Ser Lys Leu Val Lys Asp Ala Ser Ala Ser Ile Thr Met Met Asp 180 185 190 aag gag aag atc aca tac tgg gcg tca agg atg ccc ggg gga aca aag 624 Lys Glu Lys Ile Thr Tyr Trp Ala Ser Arg Met Pro Gly Gly Thr Lys 195 200 205 gag gtg ccg gtg cgc tga 642 Glu Val Pro Val Arg 210 <210> SEQ ID NO 64 <211> LENGTH: 213 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 64 Met Pro Val Met Cys Ala Val Ser Thr Arg Gly Pro Asp Ala Ala Cys 1 5 10 15 Cys Phe Met Val Ser Tyr Thr Gly Ala Tyr Thr Ile Ile Cys Arg Ala 20 25 30 Val Ala Pro Trp Pro Leu Ser Gly Phe Glu Arg Pro Ser Trp Asp Glu 35 40 45 Tyr Phe Met Leu Gln Ala Glu Leu Ala Lys Leu Arg Ser Asn Cys Met 50 55 60 Val Arg Lys Val Gly Ala Val Ile Val Arg Asp His Arg Gln Leu Ala 65 70 75 80 Thr Gly Tyr Asn Gly Thr Pro Pro Gly Val Lys Asn Cys Phe Glu Gly 85 90 95 Gly Cys Glu Arg Cys Ile Glu Arg Met Glu Gly Lys Ile Arg Ser Gly 100 105 110 Glu Gly Leu Asp Arg Cys Leu Cys Asn His Ala Glu Ala Asn Ala Ile 115 120 125 Met His Cys Ala Ile Leu Gly Ile Gly Ala Gly Gly Gly Asn Ala Thr 130 135 140 Met Tyr Thr Thr Phe Ser Pro Cys Leu Glu Cys Thr Lys Met Ala Val 145 150 155 160 Thr Ile Gly Ile Arg Arg Phe Val Cys Leu Asp Thr Tyr Pro Glu Asn 165 170 175 Ala Ser Lys Leu Val Lys Asp Ala Ser Ala Ser Ile Thr Met Met Asp 180 185 190 Lys Glu Lys Ile Thr Tyr Trp Ala Ser Arg Met Pro Gly Gly Thr Lys 195 200 205 Glu Val Pro Val Arg 210 <210> SEQ ID NO 65 <211> LENGTH: 1512 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1512) <400> SEQUENCE: 65 gtg gag acc gca cac ata acg ggc aaa tac gta gag ccc ggc gcc gtc 48 Met Glu Thr Ala His Ile Thr Gly Lys Tyr Val Glu Pro Gly Ala Val 1 5 10 15 gag agg cgc gac tac cag gtg ggc ctt gcc gag cag gcc ata cgg gaa 96 Glu Arg Arg Asp Tyr Gln Val Gly Leu Ala Glu Gln Ala Ile Arg Glu 20 25 30 aac tgc ata gtg gtg ctg cct acc ggc ctc ggc aag acg gcc gtg gcc 144 Asn Cys Ile Val Val Leu Pro Thr Gly Leu Gly Lys Thr Ala Val Ala 35 40 45 ctg cag gtg atc tcc cac tat ttg gac gaa ggc agg ggg gct ctc ttc 192 Leu Gln Val Ile Ser His Tyr Leu Asp Glu Gly Arg Gly Ala Leu Phe 50 55 60 ctt gcg ccg aca agg gtg ctg gta aac cag cac cgc cag ttc ctg ggc 240 Leu Ala Pro Thr Arg Val Leu Val Asn Gln His Arg Gln Phe Leu Gly 65 70 75 80 agg gcc ctt acc ata tcc gat att acc ctg gtc aca ggc gag gac acc 288 Arg Ala Leu Thr Ile Ser Asp Ile Thr Leu Val Thr Gly Glu Asp Thr 85 90 95 gtc ccg agg cgc aaa aaa gct tgg ggc ggc agc gtg atc tgc gcc acc 336 Val Pro Arg Arg Lys Lys Ala Trp Gly Gly Ser Val Ile Cys Ala Thr 100 105 110 ccc gag ata aca aga aac gac ata gcg cgc gga atg gtc ccg ctc gaa 384 Pro Glu Ile Thr Arg Asn Asp Ile Ala Arg Gly Met Val Pro Leu Glu 115 120 125 cag ttc ggc ctg gtt gtg ttc gac gag gcc cac agg gcg gtg ggc gac 432 Gln Phe Gly Leu Val Val Phe Asp Glu Ala His Arg Ala Val Gly Asp 130 135 140 tat gcc tat tcc gca ata gcg cgt gca gtg ggg gag aac tct aga atg 480 Tyr Ala Tyr Ser Ala Ile Ala Arg Ala Val Gly Glu Asn Ser Arg Met 145 150 155 160 atc ggc atg act gcg acc ctt cca agc gag agg gag aaa gcc gac gag 528 Ile Gly Met Thr Ala Thr Leu Pro Ser Glu Arg Glu Lys Ala Asp Glu 165 170 175 ata atg ggc act ctt ctc tca aag agc ata gca caa agg acc gaa gac 576 Ile Met Gly Thr Leu Leu Ser Lys Ser Ile Ala Gln Arg Thr Glu Asp 180 185 190 gac ccg gat gta aag ccc tac gtg cag gag acc gaa act gaa tgg ata 624 Asp Pro Asp Val Lys Pro Tyr Val Gln Glu Thr Glu Thr Glu Trp Ile 195 200 205 aag gtg gag ctg ccc ccg gag atg aag gag atc caa aag ctc ctg aag 672 Lys Val Glu Leu Pro Pro Glu Met Lys Glu Ile Gln Lys Leu Leu Lys 210 215 220 atg gcc ctc gac gaa aga tat gcg gcc ctc aag agg tgc ggc tat gat 720 Met Ala Leu Asp Glu Arg Tyr Ala Ala Leu Lys Arg Cys Gly Tyr Asp 225 230 235 240 ctc ggc tcg aac agg tcg ctc tcg gct ctg ctc cgc ctt cgc atg gtc 768 Leu Gly Ser Asn Arg Ser Leu Ser Ala Leu Leu Arg Leu Arg Met Val 245 250 255 gtt cta agc ggc aac agg cgg gcg gca aag cct ttg ttt act gcg ata 816 Val Leu Ser Gly Asn Arg Arg Ala Ala Lys Pro Leu Phe Thr Ala Ile 260 265 270 cgc atc aca tac gcg ctc aac ata ttc gag gcc cac ggg gtc acg ccg 864 Arg Ile Thr Tyr Ala Leu Asn Ile Phe Glu Ala His Gly Val Thr Pro 275 280 285 ttt cta aag ttc tgc gag agg acc gtc aag aaa aag ggc gcc ggt gtt 912 Phe Leu Lys Phe Cys Glu Arg Thr Val Lys Lys Lys Gly Ala Gly Val 290 295 300 gca gag ctg ttc gag gag gac aga aac ttt aca ggg gcc atg gcg cgc 960 Ala Glu Leu Phe Glu Glu Asp Arg Asn Phe Thr Gly Ala Met Ala Arg 305 310 315 320 gca aag gcg gcg cag gca gcc ggc atg gag cat cca aag ata cca aag 1008 Ala Lys Ala Ala Gln Ala Ala Gly Met Glu His Pro Lys Ile Pro Lys 325 330 335 ttg gaa gag gct gtg cgc ggg gcc aaa ggg aag gcg ctg gtc ttt aca 1056 Leu Glu Glu Ala Val Arg Gly Ala Lys Gly Lys Ala Leu Val Phe Thr 340 345 350 agc tac agg gac tct gtc gat tta ata cac tca aag ctg cag gct gcc 1104 Ser Tyr Arg Asp Ser Val Asp Leu Ile His Ser Lys Leu Gln Ala Ala 355 360 365 ggg ata aac tcg ggg atc ctc ata gga aag gcg gga gaa aag ggc ctc 1152 Gly Ile Asn Ser Gly Ile Leu Ile Gly Lys Ala Gly Glu Lys Gly Leu 370 375 380 aag cag aaa aaa cag gta gag act gtc gcc aag ttc cgc gac ggg gga 1200 Lys Gln Lys Lys Gln Val Glu Thr Val Ala Lys Phe Arg Asp Gly Gly 385 390 395 400 tac gac gtg ctc gta tct aca aga gtg ggc gag gag ggc ctc gac ata 1248 Tyr Asp Val Leu Val Ser Thr Arg Val Gly Glu Glu Gly Leu Asp Ile 405 410 415 tcg gag gta aac ctt gtg gta ttc tat gac aat gtc cca agc tcg ata 1296 Ser Glu Val Asn Leu Val Val Phe Tyr Asp Asn Val Pro Ser Ser Ile 420 425 430 agg tat gtg cag aga agg ggc agg acc ggc agg aag gac gcg ggc aag 1344 Arg Tyr Val Gln Arg Arg Gly Arg Thr Gly Arg Lys Asp Ala Gly Lys 435 440 445 ctg gtg gta ctg atg gca aag ggg act ata gac gag gca tac tac tgg 1392 Leu Val Val Leu Met Ala Lys Gly Thr Ile Asp Glu Ala Tyr Tyr Trp 450 455 460 ata ggc cgg cgc aag att act gcc gcc agg ggc atg ggg gac agg atg 1440 Ile Gly Arg Arg Lys Ile Thr Ala Ala Arg Gly Met Gly Asp Arg Met 465 470 475 480 aac aag tcg ctt gca gcg ggg ggc cct gcg cca aag gca gcc cca aaa 1488 Asn Lys Ser Leu Ala Ala Gly Gly Pro Ala Pro Lys Ala Ala Pro Lys 485 490 495 aag ggg ctc gag ggc tat ttc tag 1512 Lys Gly Leu Glu Gly Tyr Phe 500 <210> SEQ ID NO 66 <211> LENGTH: 503 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 66 Met Glu Thr Ala His Ile Thr Gly Lys Tyr Val Glu Pro Gly Ala Val 1 5 10 15 Glu Arg Arg Asp Tyr Gln Val Gly Leu Ala Glu Gln Ala Ile Arg Glu 20 25 30 Asn Cys Ile Val Val Leu Pro Thr Gly Leu Gly Lys Thr Ala Val Ala 35 40 45 Leu Gln Val Ile Ser His Tyr Leu Asp Glu Gly Arg Gly Ala Leu Phe 50 55 60 Leu Ala Pro Thr Arg Val Leu Val Asn Gln His Arg Gln Phe Leu Gly 65 70 75 80 Arg Ala Leu Thr Ile Ser Asp Ile Thr Leu Val Thr Gly Glu Asp Thr 85 90 95 Val Pro Arg Arg Lys Lys Ala Trp Gly Gly Ser Val Ile Cys Ala Thr 100 105 110 Pro Glu Ile Thr Arg Asn Asp Ile Ala Arg Gly Met Val Pro Leu Glu 115 120 125 Gln Phe Gly Leu Val Val Phe Asp Glu Ala His Arg Ala Val Gly Asp 130 135 140 Tyr Ala Tyr Ser Ala Ile Ala Arg Ala Val Gly Glu Asn Ser Arg Met 145 150 155 160 Ile Gly Met Thr Ala Thr Leu Pro Ser Glu Arg Glu Lys Ala Asp Glu 165 170 175 Ile Met Gly Thr Leu Leu Ser Lys Ser Ile Ala Gln Arg Thr Glu Asp 180 185 190 Asp Pro Asp Val Lys Pro Tyr Val Gln Glu Thr Glu Thr Glu Trp Ile 195 200 205 Lys Val Glu Leu Pro Pro Glu Met Lys Glu Ile Gln Lys Leu Leu Lys 210 215 220 Met Ala Leu Asp Glu Arg Tyr Ala Ala Leu Lys Arg Cys Gly Tyr Asp 225 230 235 240 Leu Gly Ser Asn Arg Ser Leu Ser Ala Leu Leu Arg Leu Arg Met Val 245 250 255 Val Leu Ser Gly Asn Arg Arg Ala Ala Lys Pro Leu Phe Thr Ala Ile 260 265 270 Arg Ile Thr Tyr Ala Leu Asn Ile Phe Glu Ala His Gly Val Thr Pro 275 280 285 Phe Leu Lys Phe Cys Glu Arg Thr Val Lys Lys Lys Gly Ala Gly Val 290 295 300 Ala Glu Leu Phe Glu Glu Asp Arg Asn Phe Thr Gly Ala Met Ala Arg 305 310 315 320 Ala Lys Ala Ala Gln Ala Ala Gly Met Glu His Pro Lys Ile Pro Lys 325 330 335 Leu Glu Glu Ala Val Arg Gly Ala Lys Gly Lys Ala Leu Val Phe Thr 340 345 350 Ser Tyr Arg Asp Ser Val Asp Leu Ile His Ser Lys Leu Gln Ala Ala 355 360 365 Gly Ile Asn Ser Gly Ile Leu Ile Gly Lys Ala Gly Glu Lys Gly Leu 370 375 380 Lys Gln Lys Lys Gln Val Glu Thr Val Ala Lys Phe Arg Asp Gly Gly 385 390 395 400 Tyr Asp Val Leu Val Ser Thr Arg Val Gly Glu Glu Gly Leu Asp Ile 405 410 415 Ser Glu Val Asn Leu Val Val Phe Tyr Asp Asn Val Pro Ser Ser Ile 420 425 430 Arg Tyr Val Gln Arg Arg Gly Arg Thr Gly Arg Lys Asp Ala Gly Lys 435 440 445 Leu Val Val Leu Met Ala Lys Gly Thr Ile Asp Glu Ala Tyr Tyr Trp 450 455 460 Ile Gly Arg Arg Lys Ile Thr Ala Ala Arg Gly Met Gly Asp Arg Met 465 470 475 480 Asn Lys Ser Leu Ala Ala Gly Gly Pro Ala Pro Lys Ala Ala Pro Lys 485 490 495 Lys Gly Leu Glu Gly Tyr Phe 500 <210> SEQ ID NO 67 <211> LENGTH: 279 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(279) <400> SEQUENCE: 67 atg gcg gac aag ata aag tgc tcg cac ata ctg gta aaa aag cag ggc 48 Met Ala Asp Lys Ile Lys Cys Ser His Ile Leu Val Lys Lys Gln Gly 1 5 10 15 gag gcg ctc gca gtg caa gag cgc ctc aag gcg ggc gaa aag ttt gga 96 Glu Ala Leu Ala Val Gln Glu Arg Leu Lys Ala Gly Glu Lys Phe Gly 20 25 30 aag ctg gca aag gag ctc tcg ata gac ggg ggc agc gca aag agg gac 144 Lys Leu Ala Lys Glu Leu Ser Ile Asp Gly Gly Ser Ala Lys Arg Asp 35 40 45 ggc agc ttg ggc tac ttt ggc agg ggc aag atg gta aag ccg ttt gag 192 Gly Ser Leu Gly Tyr Phe Gly Arg Gly Lys Met Val Lys Pro Phe Glu 50 55 60 gat gcc gcg ttc cgc ctg cag gta ggc gag gta tcc gag ccg gta aaa 240 Asp Ala Ala Phe Arg Leu Gln Val Gly Glu Val Ser Glu Pro Val Lys 65 70 75 80 tcc gag ttt ggc tac cac gtg ata aag cgc ctg gga taa 279 Ser Glu Phe Gly Tyr His Val Ile Lys Arg Leu Gly 85 90 <210> SEQ ID NO 68 <211> LENGTH: 92 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 68 Met Ala Asp Lys Ile Lys Cys Ser His Ile Leu Val Lys Lys Gln Gly 1 5 10 15 Glu Ala Leu Ala Val Gln Glu Arg Leu Lys Ala Gly Glu Lys Phe Gly 20 25 30 Lys Leu Ala Lys Glu Leu Ser Ile Asp Gly Gly Ser Ala Lys Arg Asp 35 40 45 Gly Ser Leu Gly Tyr Phe Gly Arg Gly Lys Met Val Lys Pro Phe Glu 50 55 60 Asp Ala Ala Phe Arg Leu Gln Val Gly Glu Val Ser Glu Pro Val Lys 65 70 75 80 Ser Glu Phe Gly Tyr His Val Ile Lys Arg Leu Gly 85 90 <210> SEQ ID NO 69 <211> LENGTH: 402 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(402) <400> SEQUENCE: 69 atg tct ttg tat ttt acg ata aag acg gcc aac ctg gcc ctg ccc gac 48 Met Ser Leu Tyr Phe Thr Ile Lys Thr Ala Asn Leu Ala Leu Pro Asp 1 5 10 15 gtg gta aag agg tac aac cac gtc ctg gcg tgc aag agc gag gtg atg 96 Val Val Lys Arg Tyr Asn His Val Leu Ala Cys Lys Ser Glu Val Met 20 25 30 agg gcc gag aag cag atc cag gtg tcc atc tcg tcg tcg ggc ggt ctg 144 Arg Ala Glu Lys Gln Ile Gln Val Ser Ile Ser Ser Ser Gly Gly Leu 35 40 45 gac aag tac gcg gag ctc aag cag cag ttc aac tcg agg ata acc gag 192 Asp Lys Tyr Ala Glu Leu Lys Gln Gln Phe Asn Ser Arg Ile Thr Glu 50 55 60 ttc tac cgc tcg ata gag gag ctg gag aag acg ggc gtg gtg gtc aag 240 Phe Tyr Arg Ser Ile Glu Glu Leu Glu Lys Thr Gly Val Val Val Lys 65 70 75 80 agc ata gac gag ggg ctc ctg gac ttt ccc gca aag cgc ttt ggg gac 288 Ser Ile Asp Glu Gly Leu Leu Asp Phe Pro Ala Lys Arg Phe Gly Asp 85 90 95 gac atc tgg ctg tgc tgg aag gtg ggc gag cgc gag atc aag ttc tgg 336 Asp Ile Trp Leu Cys Trp Lys Val Gly Glu Arg Glu Ile Lys Phe Trp 100 105 110 cat gaa aag gac tcg ggg ttt gac gga aga aag ccc ata gag gta agt 384 His Glu Lys Asp Ser Gly Phe Asp Gly Arg Lys Pro Ile Glu Val Ser 115 120 125 gac gag tca cta gtg tag 402 Asp Glu Ser Leu Val 130 <210> SEQ ID NO 70 <211> LENGTH: 133 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 70 Met Ser Leu Tyr Phe Thr Ile Lys Thr Ala Asn Leu Ala Leu Pro Asp 1 5 10 15 Val Val Lys Arg Tyr Asn His Val Leu Ala Cys Lys Ser Glu Val Met 20 25 30 Arg Ala Glu Lys Gln Ile Gln Val Ser Ile Ser Ser Ser Gly Gly Leu 35 40 45 Asp Lys Tyr Ala Glu Leu Lys Gln Gln Phe Asn Ser Arg Ile Thr Glu 50 55 60 Phe Tyr Arg Ser Ile Glu Glu Leu Glu Lys Thr Gly Val Val Val Lys 65 70 75 80 Ser Ile Asp Glu Gly Leu Leu Asp Phe Pro Ala Lys Arg Phe Gly Asp 85 90 95 Asp Ile Trp Leu Cys Trp Lys Val Gly Glu Arg Glu Ile Lys Phe Trp 100 105 110 His Glu Lys Asp Ser Gly Phe Asp Gly Arg Lys Pro Ile Glu Val Ser 115 120 125 Asp Glu Ser Leu Val 130 <210> SEQ ID NO 71 <211> LENGTH: 879 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(879) <400> SEQUENCE: 71 atg ctc tcc tcc tgg ctg cgc gta ata cgc gtc cgg ttc ctg ctc gcg 48 Met Leu Ser Ser Trp Leu Arg Val Ile Arg Val Arg Phe Leu Leu Ala 1 5 10 15 tcg gtg ata gcc gta tca gcg ggc ctt gcc ctc tcc tgg tgg cac ggc 96 Ser Val Ile Ala Val Ser Ala Gly Leu Ala Leu Ser Trp Trp His Gly 20 25 30 cac gga ata gac gcg ctc aca gcg gca ctc acc atg gcc gga gtg gcc 144 His Gly Ile Asp Ala Leu Thr Ala Ala Leu Thr Met Ala Gly Val Ala 35 40 45 gct ctt cat gca agc gtg gac atg ctc aac gac tac tgg gac tac aag 192 Ala Leu His Ala Ser Val Asp Met Leu Asn Asp Tyr Trp Asp Tyr Lys 50 55 60 cgc ggc ata gat acg aga acc aag agg acc ccg atg agc ggg ggg aca 240 Arg Gly Ile Asp Thr Arg Thr Lys Arg Thr Pro Met Ser Gly Gly Thr 65 70 75 80 ggg gtg ctg cca gag ggc ctg ctg agc ccc cgc cag gtg tac cgc gcc 288 Gly Val Leu Pro Glu Gly Leu Leu Ser Pro Arg Gln Val Tyr Arg Ala 85 90 95 ggc atc ata tca ctg gtg ctc ggg act gcc gcc ggc gca tac ttt gtg 336 Gly Ile Ile Ser Leu Val Leu Gly Thr Ala Ala Gly Ala Tyr Phe Val 100 105 110 atc aca acg ggg ccc gtc ata gct gcg ata ctc ggc ttt gcg gtg gtc 384 Ile Thr Thr Gly Pro Val Ile Ala Ala Ile Leu Gly Phe Ala Val Val 115 120 125 tcg att tac ttt tac tcg aca agg att gtg gac tcg ggc ctc tcc gag 432 Ser Ile Tyr Phe Tyr Ser Thr Arg Ile Val Asp Ser Gly Leu Ser Glu 130 135 140 gtg ctc gtc ggg gtc aag ggg gcg atg atc gtc ctt ggc gcc tac tac 480 Val Leu Val Gly Val Lys Gly Ala Met Ile Val Leu Gly Ala Tyr Tyr 145 150 155 160 ata cag gcg ccc gag atc acg ccg gcc gcc ctc ctc gtc ggc gcg gca 528 Ile Gln Ala Pro Glu Ile Thr Pro Ala Ala Leu Leu Val Gly Ala Ala 165 170 175 gtg ggg gcg ctg tca tct gcg gtc ctc ttt gtg gcg tcg ttt ccg gac 576 Val Gly Ala Leu Ser Ser Ala Val Leu Phe Val Ala Ser Phe Pro Asp 180 185 190 cac gac gca gac aag gag cgc ggc aga aaa acg ctg gtg ata ata ctg 624 His Asp Ala Asp Lys Glu Arg Gly Arg Lys Thr Leu Val Ile Ile Leu 195 200 205 ggc aaa aag agg gcc tcg cgc ata ctc tgg gtc ttt cca gct gtg gcg 672 Gly Lys Lys Arg Ala Ser Arg Ile Leu Trp Val Phe Pro Ala Val Ala 210 215 220 tat tca tcc gtg ata gcg ggg gtg att atc cag gtg ctg cca gtg tac 720 Tyr Ser Ser Val Ile Ala Gly Val Ile Ile Gln Val Leu Pro Val Tyr 225 230 235 240 tcc ctc gcc atg ctg ctt gcc gcc ccc ctt gcg gca ata tcg gca agg 768 Ser Leu Ala Met Leu Leu Ala Ala Pro Leu Ala Ala Ile Ser Ala Arg 245 250 255 ggc ctt gcc aaa gag tat gac ggg gac agg atc ata cgg gtc atg cgc 816 Gly Leu Ala Lys Glu Tyr Asp Gly Asp Arg Ile Ile Arg Val Met Arg 260 265 270 ggc acg ctg cgg ttc agc agg act gca ggc gcg ctg ctg gtg ctg gga 864 Gly Thr Leu Arg Phe Ser Arg Thr Ala Gly Ala Leu Leu Val Leu Gly 275 280 285 ata ctg ctt ggt tga 879 Ile Leu Leu Gly 290 <210> SEQ ID NO 72 <211> LENGTH: 292 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 72 Met Leu Ser Ser Trp Leu Arg Val Ile Arg Val Arg Phe Leu Leu Ala 1 5 10 15 Ser Val Ile Ala Val Ser Ala Gly Leu Ala Leu Ser Trp Trp His Gly 20 25 30 His Gly Ile Asp Ala Leu Thr Ala Ala Leu Thr Met Ala Gly Val Ala 35 40 45 Ala Leu His Ala Ser Val Asp Met Leu Asn Asp Tyr Trp Asp Tyr Lys 50 55 60 Arg Gly Ile Asp Thr Arg Thr Lys Arg Thr Pro Met Ser Gly Gly Thr 65 70 75 80 Gly Val Leu Pro Glu Gly Leu Leu Ser Pro Arg Gln Val Tyr Arg Ala 85 90 95 Gly Ile Ile Ser Leu Val Leu Gly Thr Ala Ala Gly Ala Tyr Phe Val 100 105 110 Ile Thr Thr Gly Pro Val Ile Ala Ala Ile Leu Gly Phe Ala Val Val 115 120 125 Ser Ile Tyr Phe Tyr Ser Thr Arg Ile Val Asp Ser Gly Leu Ser Glu 130 135 140 Val Leu Val Gly Val Lys Gly Ala Met Ile Val Leu Gly Ala Tyr Tyr 145 150 155 160 Ile Gln Ala Pro Glu Ile Thr Pro Ala Ala Leu Leu Val Gly Ala Ala 165 170 175 Val Gly Ala Leu Ser Ser Ala Val Leu Phe Val Ala Ser Phe Pro Asp 180 185 190 His Asp Ala Asp Lys Glu Arg Gly Arg Lys Thr Leu Val Ile Ile Leu 195 200 205 Gly Lys Lys Arg Ala Ser Arg Ile Leu Trp Val Phe Pro Ala Val Ala 210 215 220 Tyr Ser Ser Val Ile Ala Gly Val Ile Ile Gln Val Leu Pro Val Tyr 225 230 235 240 Ser Leu Ala Met Leu Leu Ala Ala Pro Leu Ala Ala Ile Ser Ala Arg 245 250 255 Gly Leu Ala Lys Glu Tyr Asp Gly Asp Arg Ile Ile Arg Val Met Arg 260 265 270 Gly Thr Leu Arg Phe Ser Arg Thr Ala Gly Ala Leu Leu Val Leu Gly 275 280 285 Ile Leu Leu Gly 290 <210> SEQ ID NO 73 <211> LENGTH: 1227 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1227) <400> SEQUENCE: 73 ttg agg ccc gcg gct gtg cct aca gca cgg gat att ggc gca gaa cgg 48 Met Arg Pro Ala Ala Val Pro Thr Ala Arg Asp Ile Gly Ala Glu Arg 1 5 10 15 ggc aat ctc aca ctt tgt acc ctt cat aca cat aaa tcc cgc ttg gat 96 Gly Asn Leu Thr Leu Cys Thr Leu His Thr His Lys Ser Arg Leu Asp 20 25 30 gtg cgg ctg cgc atg atc agc ggg cat gcc acg gcc gag ggt aca cag 144 Val Arg Leu Arg Met Ile Ser Gly His Ala Thr Ala Glu Gly Thr Gln 35 40 45 agg ata gcc gag atg tcc ggc gca cac cat gac aac tac aag gtg gta 192 Arg Ile Ala Glu Met Ser Gly Ala His His Asp Asn Tyr Lys Val Val 50 55 60 gac ggg ctg cac ctc tcc aac gtg ggg atg ggc acc tac ctt ggc gac 240 Asp Gly Leu His Leu Ser Asn Val Gly Met Gly Thr Tyr Leu Gly Asp 65 70 75 80 gcg gat gac gcc acc gac agg gcc gtc aca gac gcg gtc aag agg tca 288 Ala Asp Asp Ala Thr Asp Arg Ala Val Thr Asp Ala Val Lys Arg Ser 85 90 95 atc aag tcg ggg ata aac gtc ata gat acc gcg ata aac tac cgc ctc 336 Ile Lys Ser Gly Ile Asn Val Ile Asp Thr Ala Ile Asn Tyr Arg Leu 100 105 110 cag agg gcc gag cgt tcc gtg ggc agg gcc gtt aca gag ctc tca gag 384 Gln Arg Ala Glu Arg Ser Val Gly Arg Ala Val Thr Glu Leu Ser Glu 115 120 125 gag ggg ctg gta tcc agg gac cag ata ttc ata tcc aca aag gcg gga 432 Glu Gly Leu Val Ser Arg Asp Gln Ile Phe Ile Ser Thr Lys Ala Gly 130 135 140 tac gtg acc aac gat tca gag gtc tcc ctc gac ttt tgg gag tat gta 480 Tyr Val Thr Asn Asp Ser Glu Val Ser Leu Asp Phe Trp Glu Tyr Val 145 150 155 160 aaa aag gaa tac gtc ggt ggc ggc gtc ata cag tcc ggg gac ata tcc 528 Lys Lys Glu Tyr Val Gly Gly Gly Val Ile Gln Ser Gly Asp Ile Ser 165 170 175 tcg gga tac cac tgc atg aag ccc gcg tat cta gag gac cag cta aag 576 Ser Gly Tyr His Cys Met Lys Pro Ala Tyr Leu Glu Asp Gln Leu Lys 180 185 190 aga agc ctt gca aac atg aac gtc gac tgc ata gat ctt gtc tac gtg 624 Arg Ser Leu Ala Asn Met Asn Val Asp Cys Ile Asp Leu Val Tyr Val 195 200 205 cac aac ccg gtg gag ggg cag atc aag gac cgc ccc gtg ccg gag atc 672 His Asn Pro Val Glu Gly Gln Ile Lys Asp Arg Pro Val Pro Glu Ile 210 215 220 ctc gag ggg ata ggc gag gcc ttt gcc atg tac gag aaa atg cgg gag 720 Leu Glu Gly Ile Gly Glu Ala Phe Ala Met Tyr Glu Lys Met Arg Glu 225 230 235 240 gct ggc cgc ata agg tat tac ggg ctc gcc acg tgg gag tgc ttc cgg 768 Ala Gly Arg Ile Arg Tyr Tyr Gly Leu Ala Thr Trp Glu Cys Phe Arg 245 250 255 gtc gca gag ggc gac ccg cag agc atg cag ctc gaa gca gtg gta aaa 816 Val Ala Glu Gly Asp Pro Gln Ser Met Gln Leu Glu Ala Val Val Lys 260 265 270 aag gcc aag gat gcc ggc ggg gag aac cac ggc ttt agg ttc ata cag 864 Lys Ala Lys Asp Ala Gly Gly Glu Asn His Gly Phe Arg Phe Ile Gln 275 280 285 ctg cca ttc aac cag tac ttt gac cag gcc tac atg gta aag aac cag 912 Leu Pro Phe Asn Gln Tyr Phe Asp Gln Ala Tyr Met Val Lys Asn Gln 290 295 300 ggg acg ggc ggc ggc aag tca tcc ata ctg gag gcg gca gcc gcg ctg 960 Gly Thr Gly Gly Gly Lys Ser Ser Ile Leu Glu Ala Ala Ala Ala Leu 305 310 315 320 gac att ggc gtg ttc aca agc gtc ccg ttc atg cag ggc aag ctg ctc 1008 Asp Ile Gly Val Phe Thr Ser Val Pro Phe Met Gln Gly Lys Leu Leu 325 330 335 gag cct ggc ctg ctg ccg gag ttt ggc ggg ctc tcg ccc gcc ctg cgg 1056 Glu Pro Gly Leu Leu Pro Glu Phe Gly Gly Leu Ser Pro Ala Leu Arg 340 345 350 tcc ctg cag ttc atc agg tct aca ccg gga gtg ctt gcc ccc ctg ccg 1104 Ser Leu Gln Phe Ile Arg Ser Thr Pro Gly Val Leu Ala Pro Leu Pro 355 360 365 ggg cac aag tcc agc ctg cat aca gac gag aac cta aag atc atg ggc 1152 Gly His Lys Ser Ser Leu His Thr Asp Glu Asn Leu Lys Ile Met Gly 370 375 380 gtg ccc ccc att cct cct gac aag ttc ggg gag ctt gtg gcc agc ctt 1200 Val Pro Pro Ile Pro Pro Asp Lys Phe Gly Glu Leu Val Ala Ser Leu 385 390 395 400 acc tca tgg tcg ccc ggc cag aaa tag 1227 Thr Ser Trp Ser Pro Gly Gln Lys 405 <210> SEQ ID NO 74 <211> LENGTH: 408 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 74 Met Arg Pro Ala Ala Val Pro Thr Ala Arg Asp Ile Gly Ala Glu Arg 1 5 10 15 Gly Asn Leu Thr Leu Cys Thr Leu His Thr His Lys Ser Arg Leu Asp 20 25 30 Val Arg Leu Arg Met Ile Ser Gly His Ala Thr Ala Glu Gly Thr Gln 35 40 45 Arg Ile Ala Glu Met Ser Gly Ala His His Asp Asn Tyr Lys Val Val 50 55 60 Asp Gly Leu His Leu Ser Asn Val Gly Met Gly Thr Tyr Leu Gly Asp 65 70 75 80 Ala Asp Asp Ala Thr Asp Arg Ala Val Thr Asp Ala Val Lys Arg Ser 85 90 95 Ile Lys Ser Gly Ile Asn Val Ile Asp Thr Ala Ile Asn Tyr Arg Leu 100 105 110 Gln Arg Ala Glu Arg Ser Val Gly Arg Ala Val Thr Glu Leu Ser Glu 115 120 125 Glu Gly Leu Val Ser Arg Asp Gln Ile Phe Ile Ser Thr Lys Ala Gly 130 135 140 Tyr Val Thr Asn Asp Ser Glu Val Ser Leu Asp Phe Trp Glu Tyr Val 145 150 155 160 Lys Lys Glu Tyr Val Gly Gly Gly Val Ile Gln Ser Gly Asp Ile Ser 165 170 175 Ser Gly Tyr His Cys Met Lys Pro Ala Tyr Leu Glu Asp Gln Leu Lys 180 185 190 Arg Ser Leu Ala Asn Met Asn Val Asp Cys Ile Asp Leu Val Tyr Val 195 200 205 His Asn Pro Val Glu Gly Gln Ile Lys Asp Arg Pro Val Pro Glu Ile 210 215 220 Leu Glu Gly Ile Gly Glu Ala Phe Ala Met Tyr Glu Lys Met Arg Glu 225 230 235 240 Ala Gly Arg Ile Arg Tyr Tyr Gly Leu Ala Thr Trp Glu Cys Phe Arg 245 250 255 Val Ala Glu Gly Asp Pro Gln Ser Met Gln Leu Glu Ala Val Val Lys 260 265 270 Lys Ala Lys Asp Ala Gly Gly Glu Asn His Gly Phe Arg Phe Ile Gln 275 280 285 Leu Pro Phe Asn Gln Tyr Phe Asp Gln Ala Tyr Met Val Lys Asn Gln 290 295 300 Gly Thr Gly Gly Gly Lys Ser Ser Ile Leu Glu Ala Ala Ala Ala Leu 305 310 315 320 Asp Ile Gly Val Phe Thr Ser Val Pro Phe Met Gln Gly Lys Leu Leu 325 330 335 Glu Pro Gly Leu Leu Pro Glu Phe Gly Gly Leu Ser Pro Ala Leu Arg 340 345 350 Ser Leu Gln Phe Ile Arg Ser Thr Pro Gly Val Leu Ala Pro Leu Pro 355 360 365 Gly His Lys Ser Ser Leu His Thr Asp Glu Asn Leu Lys Ile Met Gly 370 375 380 Val Pro Pro Ile Pro Pro Asp Lys Phe Gly Glu Leu Val Ala Ser Leu 385 390 395 400 Thr Ser Trp Ser Pro Gly Gln Lys 405 <210> SEQ ID NO 75 <211> LENGTH: 1077 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1077) <400> SEQUENCE: 75 atg aac aac cgg ttc cag gtt atc cgg ggg gat gcc cgg gcg gtg ctg 48 Met Asn Asn Arg Phe Gln Val Ile Arg Gly Asp Ala Arg Ala Val Leu 1 5 10 15 ccc agg ctt gca aaa aag aat ggc gag cgc ggc agg tac agg ctg gcc 96 Pro Arg Leu Ala Lys Lys Asn Gly Glu Arg Gly Arg Tyr Arg Leu Ala 20 25 30 gtc act tcc ccc ccg tat tac ggg cac aga aag tac ggg tcg gat ccc 144 Val Thr Ser Pro Pro Tyr Tyr Gly His Arg Lys Tyr Gly Ser Asp Pro 35 40 45 tcc gag ctg ggc cag gag ggg acg cct gat gag ttc gtc gag gag ctg 192 Ser Glu Leu Gly Gln Glu Gly Thr Pro Asp Glu Phe Val Glu Glu Leu 50 55 60 gca ggg gtg ttc aag agc tgc atg gac ctg ctt acc gac gac ggc agc 240 Ala Gly Val Phe Lys Ser Cys Met Asp Leu Leu Thr Asp Asp Gly Ser 65 70 75 80 ctc ttc ata gtg ata ggc gac acc cgg agg cgg cgc cgg aag ctg atg 288 Leu Phe Ile Val Ile Gly Asp Thr Arg Arg Arg Arg Arg Lys Leu Met 85 90 95 gtc ccg cac cgg ctc gcg ctc aga ctt gta gac ctt ggg tac cac ttt 336 Val Pro His Arg Leu Ala Leu Arg Leu Val Asp Leu Gly Tyr His Phe 100 105 110 caa gag gat ata gtc tgg tac aag aaa aac gcg cta tca cag agc tcg 384 Gln Glu Asp Ile Val Trp Tyr Lys Lys Asn Ala Leu Ser Gln Ser Ser 115 120 125 aag cag aac ctt acg cag gcg tac gag ttt gtg ctg gtg cta tca aag 432 Lys Gln Asn Leu Thr Gln Ala Tyr Glu Phe Val Leu Val Leu Ser Lys 130 135 140 tcg gaa tcc ccc gcc ttt gac ata gac ccg ata cgc gtc cag ggc aac 480 Ser Glu Ser Pro Ala Phe Asp Ile Asp Pro Ile Arg Val Gln Gly Asn 145 150 155 160 gag gcc ctg agc ggg gtc aac agg aag ccg gag cgc gac cgg ctg cag 528 Glu Ala Leu Ser Gly Val Asn Arg Lys Pro Glu Arg Asp Arg Leu Gln 165 170 175 ttc tcc ccc ggg agg agg gac cct gaa gcc ata ggg agg att gca gca 576 Phe Ser Pro Gly Arg Arg Asp Pro Glu Ala Ile Gly Arg Ile Ala Ala 180 185 190 gtg ata cac ggc tcg tcc ccc gag acg ccg ttt gac gag ctg cca acc 624 Val Ile His Gly Ser Ser Pro Glu Thr Pro Phe Asp Glu Leu Pro Thr 195 200 205 acc gag gag ata tcg cgg gcc cac ggg tat gac ccc gaa aag cac tgc 672 Thr Glu Glu Ile Ser Arg Ala His Gly Tyr Asp Pro Glu Lys His Cys 210 215 220 ccg aca tgc tac cgc aag ttc aaa agg cat gcg acg cgc aag cgg ata 720 Pro Thr Cys Tyr Arg Lys Phe Lys Arg His Ala Thr Arg Lys Arg Ile 225 230 235 240 ggg ggc cac gag cac tat ccg ata ttt gca gca tgc aac ccc cgg ggc 768 Gly Gly His Glu His Tyr Pro Ile Phe Ala Ala Cys Asn Pro Arg Gly 245 250 255 aag aac cct ggg aac gtc tgg gag ata tcc aca aag gcg cac cac ggc 816 Lys Asn Pro Gly Asn Val Trp Glu Ile Ser Thr Lys Ala His His Gly 260 265 270 aac gag cac ttt gcg gtg ttc cca gaa gac ctc gta tcc cgg ata gta 864 Asn Glu His Phe Ala Val Phe Pro Glu Asp Leu Val Ser Arg Ile Val 275 280 285 aag ttt gcc aca aga gag ggc gac tat gtg ctg gat ccg ttt gcg gga 912 Lys Phe Ala Thr Arg Glu Gly Asp Tyr Val Leu Asp Pro Phe Ala Gly 290 295 300 agg ggc aca acg ggg ata gtc tcg gcg tgc ctc aag agg ggc ttt acg 960 Arg Gly Thr Thr Gly Ile Val Ser Ala Cys Leu Lys Arg Gly Phe Thr 305 310 315 320 gga ata gac ctg tat cct gcc aac gtg gac agg acc cgg cgc aat gtg 1008 Gly Ile Asp Leu Tyr Pro Ala Asn Val Asp Arg Thr Arg Arg Asn Val 325 330 335 aaa gat tct gcg gac tcg aag ctg cca aaa aag gtg cta gac cag ata 1056 Lys Asp Ser Ala Asp Ser Lys Leu Pro Lys Lys Val Leu Asp Gln Ile 340 345 350 atg ccc gag gga aca cgc tga 1077 Met Pro Glu Gly Thr Arg 355 <210> SEQ ID NO 76 <211> LENGTH: 358 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 76 Met Asn Asn Arg Phe Gln Val Ile Arg Gly Asp Ala Arg Ala Val Leu 1 5 10 15 Pro Arg Leu Ala Lys Lys Asn Gly Glu Arg Gly Arg Tyr Arg Leu Ala 20 25 30 Val Thr Ser Pro Pro Tyr Tyr Gly His Arg Lys Tyr Gly Ser Asp Pro 35 40 45 Ser Glu Leu Gly Gln Glu Gly Thr Pro Asp Glu Phe Val Glu Glu Leu 50 55 60 Ala Gly Val Phe Lys Ser Cys Met Asp Leu Leu Thr Asp Asp Gly Ser 65 70 75 80 Leu Phe Ile Val Ile Gly Asp Thr Arg Arg Arg Arg Arg Lys Leu Met 85 90 95 Val Pro His Arg Leu Ala Leu Arg Leu Val Asp Leu Gly Tyr His Phe 100 105 110 Gln Glu Asp Ile Val Trp Tyr Lys Lys Asn Ala Leu Ser Gln Ser Ser 115 120 125 Lys Gln Asn Leu Thr Gln Ala Tyr Glu Phe Val Leu Val Leu Ser Lys 130 135 140 Ser Glu Ser Pro Ala Phe Asp Ile Asp Pro Ile Arg Val Gln Gly Asn 145 150 155 160 Glu Ala Leu Ser Gly Val Asn Arg Lys Pro Glu Arg Asp Arg Leu Gln 165 170 175 Phe Ser Pro Gly Arg Arg Asp Pro Glu Ala Ile Gly Arg Ile Ala Ala 180 185 190 Val Ile His Gly Ser Ser Pro Glu Thr Pro Phe Asp Glu Leu Pro Thr 195 200 205 Thr Glu Glu Ile Ser Arg Ala His Gly Tyr Asp Pro Glu Lys His Cys 210 215 220 Pro Thr Cys Tyr Arg Lys Phe Lys Arg His Ala Thr Arg Lys Arg Ile 225 230 235 240 Gly Gly His Glu His Tyr Pro Ile Phe Ala Ala Cys Asn Pro Arg Gly 245 250 255 Lys Asn Pro Gly Asn Val Trp Glu Ile Ser Thr Lys Ala His His Gly 260 265 270 Asn Glu His Phe Ala Val Phe Pro Glu Asp Leu Val Ser Arg Ile Val 275 280 285 Lys Phe Ala Thr Arg Glu Gly Asp Tyr Val Leu Asp Pro Phe Ala Gly 290 295 300 Arg Gly Thr Thr Gly Ile Val Ser Ala Cys Leu Lys Arg Gly Phe Thr 305 310 315 320 Gly Ile Asp Leu Tyr Pro Ala Asn Val Asp Arg Thr Arg Arg Asn Val 325 330 335 Lys Asp Ser Ala Asp Ser Lys Leu Pro Lys Lys Val Leu Asp Gln Ile 340 345 350 Met Pro Glu Gly Thr Arg 355 <210> SEQ ID NO 77 <211> LENGTH: 468 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(468) <400> SEQUENCE: 77 atg cgg ctg ccc cgg cgc cga ctt aaa atc gtt gta gga tgc ggc gcc 48 Met Arg Leu Pro Arg Arg Arg Leu Lys Ile Val Val Gly Cys Gly Ala 1 5 10 15 gca gat gca ttg ccc gcc tta tac acc gcc cgg gat cgg ccg cct tgc 96 Ala Asp Ala Leu Pro Ala Leu Tyr Thr Ala Arg Asp Arg Pro Pro Cys 20 25 30 agc aca cgc agt ata aac ggg ggc ccg ggc ggc gcg tat cac atg tgg 144 Ser Thr Arg Ser Ile Asn Gly Gly Pro Gly Gly Ala Tyr His Met Trp 35 40 45 ata aag gac gaa ttc ctc ggc ccg ggc aac aag atg agg ctg ctc tac 192 Ile Lys Asp Glu Phe Leu Gly Pro Gly Asn Lys Met Arg Leu Leu Tyr 50 55 60 ctg ata ctg ccc atc tat ggg tat atc ttt ctg gag tac tat ccg ttc 240 Leu Ile Leu Pro Ile Tyr Gly Tyr Ile Phe Leu Glu Tyr Tyr Pro Phe 65 70 75 80 ttt ccc tgg atg gcc acc tac tgg tgg tca gta gct ctc agc ccc ccg 288 Phe Pro Trp Met Ala Thr Tyr Trp Trp Ser Val Ala Leu Ser Pro Pro 85 90 95 ata gtg ccc acg cat tat gcc ggg gag gcc ctg ggg cgg ctg atc ggg 336 Ile Val Pro Thr His Tyr Ala Gly Glu Ala Leu Gly Arg Leu Ile Gly 100 105 110 gat cac gta ttg ttt ggc atc acc aca aag tac gtc tat gcg gca ata 384 Asp His Val Leu Phe Gly Ile Thr Thr Lys Tyr Val Tyr Ala Ala Ile 115 120 125 tgg ctc ggc atg gcc cat ggg ata atc ctg ctg gca ggg cgc ctc cgg 432 Trp Leu Gly Met Ala His Gly Ile Ile Leu Leu Ala Gly Arg Leu Arg 130 135 140 gga cct agg cag gcg cca cgg acg ggc atc cca tag 468 Gly Pro Arg Gln Ala Pro Arg Thr Gly Ile Pro 145 150 155 <210> SEQ ID NO 78 <211> LENGTH: 155 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 78 Met Arg Leu Pro Arg Arg Arg Leu Lys Ile Val Val Gly Cys Gly Ala 1 5 10 15 Ala Asp Ala Leu Pro Ala Leu Tyr Thr Ala Arg Asp Arg Pro Pro Cys 20 25 30 Ser Thr Arg Ser Ile Asn Gly Gly Pro Gly Gly Ala Tyr His Met Trp 35 40 45 Ile Lys Asp Glu Phe Leu Gly Pro Gly Asn Lys Met Arg Leu Leu Tyr 50 55 60 Leu Ile Leu Pro Ile Tyr Gly Tyr Ile Phe Leu Glu Tyr Tyr Pro Phe 65 70 75 80 Phe Pro Trp Met Ala Thr Tyr Trp Trp Ser Val Ala Leu Ser Pro Pro 85 90 95 Ile Val Pro Thr His Tyr Ala Gly Glu Ala Leu Gly Arg Leu Ile Gly 100 105 110 Asp His Val Leu Phe Gly Ile Thr Thr Lys Tyr Val Tyr Ala Ala Ile 115 120 125 Trp Leu Gly Met Ala His Gly Ile Ile Leu Leu Ala Gly Arg Leu Arg 130 135 140 Gly Pro Arg Gln Ala Pro Arg Thr Gly Ile Pro 145 150 155 <210> SEQ ID NO 79 <211> LENGTH: 1779 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(1779) <400> SEQUENCE: 79 ttg aag ctg caa ggc aag act gcc gtg atc acc ggc agt ggt acc ggg 48 Met Lys Leu Gln Gly Lys Thr Ala Val Ile Thr Gly Ser Gly Thr Gly 1 5 10 15 atc ggg ctg gcg gtg gca agg aaa ttt gcc gag aac ggg gcc agc gtg 96 Ile Gly Leu Ala Val Ala Arg Lys Phe Ala Glu Asn Gly Ala Ser Val 20 25 30 gta ata ctc gga agg aga aag gag ccc ctc gat gag gca gca gca gag 144 Val Ile Leu Gly Arg Arg Lys Glu Pro Leu Asp Glu Ala Ala Ala Glu 35 40 45 ctc aaa aag ata gcg gaa tct gca ggc tgc ggg gcc tcg atc agg ata 192 Leu Lys Lys Ile Ala Glu Ser Ala Gly Cys Gly Ala Ser Ile Arg Ile 50 55 60 ttc gcc ggg gtg gac gtg gcc gac gaa tcc gcg ata acg aaa atg ttc 240 Phe Ala Gly Val Asp Val Ala Asp Glu Ser Ala Ile Thr Lys Met Phe 65 70 75 80 gac gag ctg tcc agc tca ggt gta acc gtg gac ata ctg gtg aac aat 288 Asp Glu Leu Ser Ser Ser Gly Val Thr Val Asp Ile Leu Val Asn Asn 85 90 95 gcc ggc gtg tcg ggg ccc gtc acg tgc ttt gcc aac aat gat cta gaa 336 Ala Gly Val Ser Gly Pro Val Thr Cys Phe Ala Asn Asn Asp Leu Glu 100 105 110 gag ttc cgc ggg gca gtc gac ata cac ctg acc ggc tcc ttc tgg aca 384 Glu Phe Arg Gly Ala Val Asp Ile His Leu Thr Gly Ser Phe Trp Thr 115 120 125 tcg agg gag gcc ctc aag gtc atg aaa aag ggc tcc aag att gtc acc 432 Ser Arg Glu Ala Leu Lys Val Met Lys Lys Gly Ser Lys Ile Val Thr 130 135 140 atg act acg ttt ttt gca gaa gag agg cca ctc gag cag agg ccg tac 480 Met Thr Thr Phe Phe Ala Glu Glu Arg Pro Leu Glu Gln Arg Pro Tyr 145 150 155 160 agg ttc cgc gac ccg tat aca acc gca cag ggc gca aag aac agg ctc 528 Arg Phe Arg Asp Pro Tyr Thr Thr Ala Gln Gly Ala Lys Asn Arg Leu 165 170 175 gcc gag gcg atg tcg tgg gat ctt tta gac cgc ggg ata aca tcg ata 576 Ala Glu Ala Met Ser Trp Asp Leu Leu Asp Arg Gly Ile Thr Ser Ile 180 185 190 gcg acc aac ccc ggc ccc gtc cat tct gac agg ata tac aag acg gta 624 Ala Thr Asn Pro Gly Pro Val His Ser Asp Arg Ile Tyr Lys Thr Val 195 200 205 tac ccg agg gcg gca ctc gag ttt gtc agg gtt tca ggg ttt gag gac 672 Tyr Pro Arg Ala Ala Leu Glu Phe Val Arg Val Ser Gly Phe Glu Asp 210 215 220 ctg cag cca gaa gaa gtc gag gtg gca ggc ggc agg cta atc cac ctg 720 Leu Gln Pro Glu Glu Val Glu Val Ala Gly Gly Arg Leu Ile His Leu 225 230 235 240 ctc ggc gcg gac gac gat gca aga aaa aaa ggc ata gca gag gcc gca 768 Leu Gly Ala Asp Asp Asp Ala Arg Lys Lys Gly Ile Ala Glu Ala Ala 245 250 255 gag cac ttt gcc aag cta aag ccc gtg gat ccc gca aag cta gag gcc 816 Glu His Phe Ala Lys Leu Lys Pro Val Asp Pro Ala Lys Leu Glu Ala 260 265 270 acc ctt gat gcc ctg ctc gca aag atc aag ggg ata gcc gaa aag ata 864 Thr Leu Asp Ala Leu Leu Ala Lys Ile Lys Gly Ile Ala Glu Lys Ile 275 280 285 cag gcc aac act gca agg atg ata cca gac ggg gag ttt ctc tcc cag 912 Gln Ala Asn Thr Ala Arg Met Ile Pro Asp Gly Glu Phe Leu Ser Gln 290 295 300 gac cag gtg gcc gag acg gta ctc gcc ctc tgc gat gac aag atg gcc 960 Asp Gln Val Ala Glu Thr Val Leu Ala Leu Cys Asp Asp Lys Met Ala 305 310 315 320 aag acg gta aac ggc cgc gta atc ccc gcc gac agg gta ttc tac ccg 1008 Lys Thr Val Asn Gly Arg Val Ile Pro Ala Asp Arg Val Phe Tyr Pro 325 330 335 gta agg gcg cat gtg gcc aat gcc gct ccg cgc gtg ccc ccg cac gac 1056 Val Arg Ala His Val Ala Asn Ala Ala Pro Arg Val Pro Pro His Asp 340 345 350 tat tcc ggg gga tgc gtc cta ttc atg ata gat gca gca gac gac agg 1104 Tyr Ser Gly Gly Cys Val Leu Phe Met Ile Asp Ala Ala Asp Asp Arg 355 360 365 gat gta gaa agg gcg acc gcc ctg gca tcc cat gtg gaa agc cac ggg 1152 Asp Val Glu Arg Ala Thr Ala Leu Ala Ser His Val Glu Ser His Gly 370 375 380 ggc acg gca gtc tgc ata gtc tca gaa gac tcg ccc cgc gcg gca aag 1200 Gly Thr Ala Val Cys Ile Val Ser Glu Asp Ser Pro Arg Ala Ala Lys 385 390 395 400 gag atg ata gcg tca aag ttc cac tcg cat gcg agc cac ata gac aag 1248 Glu Met Ile Ala Ser Lys Phe His Ser His Ala Ser His Ile Asp Lys 405 410 415 gta gac gag ata aac agg tgg ctg agc gct gca tca aca aag ata ggc 1296 Val Asp Glu Ile Asn Arg Trp Leu Ser Ala Ala Ser Thr Lys Ile Gly 420 425 430 ccc ata tct gca gtg gtc cac ctg tcc ggc agg atg cca aaa tcc ggc 1344 Pro Ile Ser Ala Val Val His Leu Ser Gly Arg Met Pro Lys Ser Gly 435 440 445 agc cta atg gat ctc tcc aga aaa gaa tgg gac gcg ctg gtt gac agg 1392 Ser Leu Met Asp Leu Ser Arg Lys Glu Trp Asp Ala Leu Val Asp Arg 450 455 460 ttc ata ggg acg ccg gct gcc gtc ctg cac agg tcg ctt gag cac ttt 1440 Phe Ile Gly Thr Pro Ala Ala Val Leu His Arg Ser Leu Glu His Phe 465 470 475 480 gca ccc ggc ggg cgc aag gac ccc cgt ttg ttc aag ggc aag agc ggc 1488 Ala Pro Gly Gly Arg Lys Asp Pro Arg Leu Phe Lys Gly Lys Ser Gly 485 490 495 gtc atc gtg ata ata ggc ccc gac ctg ccc gcg ggg aaa aag gcc tcc 1536 Val Ile Val Ile Ile Gly Pro Asp Leu Pro Ala Gly Lys Lys Ala Ser 500 505 510 ggc gcc gag agg gca agg gcg gag atc ttc cgg ggt gcg ctc agg ccg 1584 Gly Ala Glu Arg Ala Arg Ala Glu Ile Phe Arg Gly Ala Leu Arg Pro 515 520 525 ctg acg act aca gtc aac cag gag ctc agc gat gtg cta aag tca aac 1632 Leu Thr Thr Thr Val Asn Gln Glu Leu Ser Asp Val Leu Lys Ser Asn 530 535 540 gtg cgc ctg ttt acc atc ctt ccc ggc agg gcg gac ggg ggc gag acc 1680 Val Arg Leu Phe Thr Ile Leu Pro Gly Arg Ala Asp Gly Gly Glu Thr 545 550 555 560 gat gat tcc cgc ata tct gct gca atc gac tac ttt ctg acc ccc gag 1728 Asp Asp Ser Arg Ile Ser Ala Ala Ile Asp Tyr Phe Leu Thr Pro Glu 565 570 575 gct gtc tcg tcc ggc gag gtc ata ttc tgc gta gac gag aac agg ggc 1776 Ala Val Ser Ser Gly Glu Val Ile Phe Cys Val Asp Glu Asn Arg Gly 580 585 590 tag 1779 <210> SEQ ID NO 80 <211> LENGTH: 592 <212> TYPE: PRT <213> ORGANISM: Cenarchaeum symbiosum <400> SEQUENCE: 80 Met Lys Leu Gln Gly Lys Thr Ala Val Ile Thr Gly Ser Gly Thr Gly 1 5 10 15 Ile Gly Leu Ala Val Ala Arg Lys Phe Ala Glu Asn Gly Ala Ser Val 20 25 30 Val Ile Leu Gly Arg Arg Lys Glu Pro Leu Asp Glu Ala Ala Ala Glu 35 40 45 Leu Lys Lys Ile Ala Glu Ser Ala Gly Cys Gly Ala Ser Ile Arg Ile 50 55 60 Phe Ala Gly Val Asp Val Ala Asp Glu Ser Ala Ile Thr Lys Met Phe 65 70 75 80 Asp Glu Leu Ser Ser Ser Gly Val Thr Val Asp Ile Leu Val Asn Asn 85 90 95 Ala Gly Val Ser Gly Pro Val Thr Cys Phe Ala Asn Asn Asp Leu Glu 100 105 110 Glu Phe Arg Gly Ala Val Asp Ile His Leu Thr Gly Ser Phe Trp Thr 115 120 125 Ser Arg Glu Ala Leu Lys Val Met Lys Lys Gly Ser Lys Ile Val Thr 130 135 140 Met Thr Thr Phe Phe Ala Glu Glu Arg Pro Leu Glu Gln Arg Pro Tyr 145 150 155 160 Arg Phe Arg Asp Pro Tyr Thr Thr Ala Gln Gly Ala Lys Asn Arg Leu 165 170 175 Ala Glu Ala Met Ser Trp Asp Leu Leu Asp Arg Gly Ile Thr Ser Ile 180 185 190 Ala Thr Asn Pro Gly Pro Val His Ser Asp Arg Ile Tyr Lys Thr Val 195 200 205 Tyr Pro Arg Ala Ala Leu Glu Phe Val Arg Val Ser Gly Phe Glu Asp 210 215 220 Leu Gln Pro Glu Glu Val Glu Val Ala Gly Gly Arg Leu Ile His Leu 225 230 235 240 Leu Gly Ala Asp Asp Asp Ala Arg Lys Lys Gly Ile Ala Glu Ala Ala 245 250 255 Glu His Phe Ala Lys Leu Lys Pro Val Asp Pro Ala Lys Leu Glu Ala 260 265 270 Thr Leu Asp Ala Leu Leu Ala Lys Ile Lys Gly Ile Ala Glu Lys Ile 275 280 285 Gln Ala Asn Thr Ala Arg Met Ile Pro Asp Gly Glu Phe Leu Ser Gln 290 295 300 Asp Gln Val Ala Glu Thr Val Leu Ala Leu Cys Asp Asp Lys Met Ala 305 310 315 320 Lys Thr Val Asn Gly Arg Val Ile Pro Ala Asp Arg Val Phe Tyr Pro 325 330 335 Val Arg Ala His Val Ala Asn Ala Ala Pro Arg Val Pro Pro His Asp 340 345 350 Tyr Ser Gly Gly Cys Val Leu Phe Met Ile Asp Ala Ala Asp Asp Arg 355 360 365 Asp Val Glu Arg Ala Thr Ala Leu Ala Ser His Val Glu Ser His Gly 370 375 380 Gly Thr Ala Val Cys Ile Val Ser Glu Asp Ser Pro Arg Ala Ala Lys 385 390 395 400 Glu Met Ile Ala Ser Lys Phe His Ser His Ala Ser His Ile Asp Lys 405 410 415 Val Asp Glu Ile Asn Arg Trp Leu Ser Ala Ala Ser Thr Lys Ile Gly 420 425 430 Pro Ile Ser Ala Val Val His Leu Ser Gly Arg Met Pro Lys Ser Gly 435 440 445 Ser Leu Met Asp Leu Ser Arg Lys Glu Trp Asp Ala Leu Val Asp Arg 450 455 460 Phe Ile Gly Thr Pro Ala Ala Val Leu His Arg Ser Leu Glu His Phe 465 470 475 480 Ala Pro Gly Gly Arg Lys Asp Pro Arg Leu Phe Lys Gly Lys Ser Gly 485 490 495 Val Ile Val Ile Ile Gly Pro Asp Leu Pro Ala Gly Lys Lys Ala Ser 500 505 510 Gly Ala Glu Arg Ala Arg Ala Glu Ile Phe Arg Gly Ala Leu Arg Pro 515 520 525 Leu Thr Thr Thr Val Asn Gln Glu Leu Ser Asp Val Leu Lys Ser Asn 530 535 540 Val Arg Leu Phe Thr Ile Leu Pro Gly Arg Ala Asp Gly Gly Glu Thr 545 550 555 560 Asp Asp Ser Arg Ile Ser Ala Ala Ile Asp Tyr Phe Leu Thr Pro Glu 565 570 575 Ala Val Ser Ser Gly Glu Val Ile Phe Cys Val Asp Glu Asn Arg Gly 580 585 590 <210> SEQ ID NO 81 <211> LENGTH: 40 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 81 aagctagact tttaattggg atccggcggg gcggcgcatg 40 <210> SEQ ID NO 82 <211> LENGTH: 40 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 82 aagctaaact tttaattggg atccggcgag ccggcgcgtg 40 <210> SEQ ID NO 83 <211> LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 83 ggaaactttg attatacggg cgtgctgccc cggggcccat g 41 <210> SEQ ID NO 84 <211> LENGTH: 41 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 84 ggaaactttg attatacggg cgtacattcc cggggcccat g 41 <210> SEQ ID NO 85 <211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 85 aaggcaaggt aataatagcc tgccgtctgt aacggccgta tg 42 <210> SEQ ID NO 86 <211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 86 acggcaaggt aataatagcc tgccgtccgt acctgccgta tg 42 <210> SEQ ID NO 87 <211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 87 catggaacta gatattaacc ggttccgcgg atcccatgca tg 42 <210> SEQ ID NO 88 <211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 88 catggaacta gataataacc ggtcccgcgg gtacaatgca tg 42 <210> SEQ ID NO 89 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 89 ataccgagaa gttatagcag ggtatggaat gtgcgcgcgc atg 43 <210> SEQ ID NO 90 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 90 agcacgacaa gttatagcag ggtacaaagg agcagcgcac atg 43 <210> SEQ ID NO 91 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarcheaum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 91 atccgccctg attaaattat ggggggagcg gcctgctgcc gtg 43 <210> SEQ ID NO 92 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 92 atccggcctc attaaattac ggggggtaca acctgctgcc gtg 43 <210> SEQ ID NO 93 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 93 ccttcataca cataaatccc gcttggatgt gcggctgcgc atg 43 <210> SEQ ID NO 94 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 94 acttcataca cataaatccc gcctgaacgg tcgtccgcgc atg 43 <210> SEQ ID NO 95 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (10)...(15) <400> SEQUENCE: 95 ggcatatacc ataatatgcc gggcggtggc accatggccg ttg 43 <210> SEQ ID NO 96 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 96 ccgcatatac cataatatgc cgggcggggg caggctgccc gtg 43 <210> SEQ ID NO 97 <211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 97 tgtacgaaac cataaaacaa caggccgcgt cagggccgcg cgtg 44 <210> SEQ ID NO 98 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 98 gggtagaaac cataaaacaa caggccgcgg cagggcgcgc gtg 43 <210> SEQ ID NO 99 <211> LENGTH: 42 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (9)...(14) <400> SEQUENCE: 99 acacgcagta taaacggggg cccgggcggc gcgtatcaca tg 42 <210> SEQ ID NO 100 <211> LENGTH: 43 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 100 atacacgtgg tataaacaga ggccggacgg cgcggaccac atg 43 <210> SEQ ID NO 101 <211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 101 gcgatagtta tttaaaacta ggatgccgat cacggatcgt ccca 44 <210> SEQ ID NO 102 <211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 102 gcgatagtta tttaaaacta ggatgccggg cacccgtcgt ccca 44 <210> SEQ ID NO 103 <211> LENGTH: 44 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 103 ccgggccccg gttaaaatag cgcacgggcg gatcctgacc aatg 44 <210> SEQ ID NO 104 <211> LENGTH: 45 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 104 ccgggccccg gttaaaatag agtgcggccg ggcaccggat caatg 45 <210> SEQ ID NO 105 <211> LENGTH: 51 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 105 gcgtcgatag aataaatacg cgcagggggc cccgtggcgc gatcgcccgt g 51 <210> SEQ ID NO 106 <211> LENGTH: 47 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 106 gcgtcgatag aataaatacg cgcggggccg cggtgcgatc gcccgtg 47 <210> SEQ ID NO 107 <211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 107 atttcaacta cataaatgcc tagttacgca gaaatagcaa acgacgtact tcgactaatg 60 <210> SEQ ID NO 108 <211> LENGTH: 60 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 108 acttcaacta cataaatgcc tagctacgca gaaatatcaa acaaagtact tcgactaatg 60 <210> SEQ ID NO 109 <211> LENGTH: 67 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 109 acggcaggct attattacct tgccttgcgt tgtatagtat gccttatgcg gggtgcggca 60 ggggatg 67 <210> SEQ ID NO 110 <211> LENGTH: 66 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 110 acggcaggct attattacct tgccgtgtgt acagggcatg ccggatgagg gggcctgccg 60 ggagtg 66 <210> SEQ ID NO 111 <211> LENGTH: 121 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 111 ctacaacgat tttaagtcgg cgccggggca gccgcataga atgtgtatga cccgtaggat 60 cgcgcggccc gcctgctgcg cagatctgtc cgtccagcct gatgtggggc aggcaacatg 20 a 21 <210> SEQ ID NO 112 <211> LENGTH: 98 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 112 ctacaaagat tttaagacgg cgcgggtgcc gcggtacaag atgaatacga cttgtcggat 60 cgcgcagggg cagatggatg gcacgggggc ctatcttg 98 <210> SEQ ID NO 113 <211> LENGTH: 236 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 113 tcggcgatgg tttatatgcc catggacggg ccgatccgat cgtacgtgac gcaagagcgg 60 cgcttgcgat gaatgcatgg tattgtacca tattgtgatt cgctggcctc cagttacgca 20 cacagaatga gggtatgatc gaagggtcat atctgagatg tgaagattat gtgcattctg 80 ttcaattcca aaagtacaag cgtacttaac aaaaaaaaaa taatccaatt atgaat 36 <210> SEQ ID NO 114 <211> LENGTH: 235 <212> TYPE: DNA <213> ORGANISM: Cenarchaeum symbiosum <220> FEATURE: <221> NAME/KEY: TATA_signal <222> LOCATION: (11)...(16) <400> SEQUENCE: 114 ccggcgatgg tttatatgcc catggacaag gcgatccgat cgtacgtgac gcaagagcgg 60 cgcttgcgat gaagccatgg tattgtacca ttttgtgatt cgcaggcctc cagttacgca 20 cacagaatga ggatctgatc gaagggtcat atctgagatg tgaagattat gtgcattccg 80 ttcaattcca aaagtacagg cgtactttga aaaaaaaaat aatccaaata agaat 35 <210> SEQ ID NO 115 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 115 gtgctccccc gccaattcct 20 <210> SEQ ID NO 116 <211> LENGTH: 15 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 116 ctttccctca cggta 15 <210> SEQ ID NO 117 <211> LENGTH: 19 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 117 ctattgccgt ctttacacc 19 <210> SEQ ID NO 118 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 118 gaatccgccc ccgactatct t 21 <210> SEQ ID NO 119 <211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 119 catggcttag tatcaatc 18 <210> SEQ ID NO 120 <211> LENGTH: 23 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <221> NAME/KEY: modified_base <222> LOCATION: (3)...(3) <223> OTHER INFORMATION: I <221> NAME/KEY: modified_base <222> LOCATION: (12)...(12) <223> OTHER INFORMATION: I <400> SEQUENCE: 120 acntacaacg gngacgaytt tga 23 <210> SEQ ID NO 121 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 121 caccccgaar tagttyttyt t 21 <210> SEQ ID NO 122 <211> LENGTH: 19 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 122 acacttcaac tatttcctg 19 <210> SEQ ID NO 123 <211> LENGTH: 19 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: Oligonucleotide <400> SEQUENCE: 123 acactttgac tatttcgtg 19 

What is claimed is:
 1. A computer readable medium having stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQID NOS. 1, 2, 5,9, 13,25,27,29, 31,33, 37,41,45, 57, 59, 61, 63, 65, 67,71, 75, 79, 3, 7, 11, 15, 17, 19,21,23, 35, 39, 43,47,49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOS. 6, 10, 14, 26,28, 30, 32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24,36,40,44,48, 50, 52, 54, 56, 70, 74, and
 78. 2. A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a sequence selected from the group consisting of a nucleic acid code of SEQID NOS. 1,2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39,43,47,49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOS. 6, 10, 14,26,28,30, 32, 34, 38, 42,46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and
 78. 3. The computer system of claim 2 further comprising a sequence comparer and a data storage device having reference sequences stored thereon.
 4. The computer system of claim 3 wherein said sequence comparer comprises a computer program which indicates polymorphisms.
 5. The computer system of claim 2 further comprising an identifier which identifies features in said sequence.
 6. A method for comparing a first sequence to a reference sequence wherein said first sequence is selected from the group consisting of a nucleic acid code of SEQID NOS. 1,2, 5,9, 13,25,27,29, 31,33, 37,41,45, 57, 59, 61,63,65,67,71,75, 79, 3,7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47, 49, 51, 53, 55, 69, 73 and 77 and a polypeptide code of SEQ ID NOS. 6, 10, 14,26,28, 30, 32, 34, 38, 42,46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising the steps of reading said first sequence and said reference sequence through use of a computer program which compares sequences; and determining differences between said first sequence and said reference sequence with said computer program.
 7. The method of claim 6, wherein said step of determining differences between the first sequence and the reference sequence comprises identifying polymorphisms.
 8. A method for identifying a feature in a sequence selected from the group consisting of a nucleic acid code of SEQID NOS. 1,2, 5, 9, 13, 25, 27, 29, 31, 33, 37, 41, 45, 57, 59, 61, 63, 65, 67, 71, 75, 79, 3, 7, 11, 15, 17, 19, 21, 23, 35, 39, 43, 47,49, 51, 53,55,69,73 and 77 and a polypeptide code of SEQ ID NOS. 6, 10, 14,26,28,30,32, 34, 38, 42, 46, 58, 60, 62, 64, 66, 68, 72, 76, 80, 4, 8, 12, 16, 18, 20, 22, 24, 36, 40, 44, 48, 50, 52, 54, 56, 70, 74, and 78 comprising the steps of: reading said sequence through the use of a computer program which identifies features in sequences; and identifying features in said sequence with said computer program. 